ES478806A1 - Un metodo de preparacion de eritrocitos c3-c4-sensibilizados. - Google Patents
Un metodo de preparacion de eritrocitos c3-c4-sensibilizados.Info
- Publication number
- ES478806A1 ES478806A1 ES478806A ES478806A ES478806A1 ES 478806 A1 ES478806 A1 ES 478806A1 ES 478806 A ES478806 A ES 478806A ES 478806 A ES478806 A ES 478806A ES 478806 A1 ES478806 A1 ES 478806A1
- Authority
- ES
- Spain
- Prior art keywords
- cells
- sensitized
- igg
- igm
- erythrocytes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000003743 erythrocyte Anatomy 0.000 title abstract 4
- 230000000295 complement effect Effects 0.000 title abstract 2
- 108060003951 Immunoglobulin Proteins 0.000 title 1
- 102000018358 immunoglobulin Human genes 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 4
- 241001465754 Metazoa Species 0.000 abstract 2
- 102000004506 Blood Proteins Human genes 0.000 abstract 1
- 108010017384 Blood Proteins Proteins 0.000 abstract 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 abstract 1
- 102000003839 Human Proteins Human genes 0.000 abstract 1
- 108090000144 Human Proteins Proteins 0.000 abstract 1
- 206010070834 Sensitisation Diseases 0.000 abstract 1
- 230000004520 agglutination Effects 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 239000006285 cell suspension Substances 0.000 abstract 1
- 230000000415 inactivating effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000008313 sensitization Effects 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- G01N33/555—Red blood cell
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2496/00—Reference solutions for assays of biological material
- G01N2496/05—Reference solutions for assays of biological material containing blood cells or plasma
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/821—Chemistry: analytical and immunological testing involving complement factors or complement systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/825—Pretreatment for removal of interfering factors from sample
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/826—Additives, e.g. buffers, diluents, preservatives
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Mycology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Un surtido de cuatro suspensiones celulares que contiene eritrocitos mono-específicamente sensibilizados con IgG, Img, C3 y C4. Las suspensiones proporcionan células de control para determinar la pureza y concentración de los anticuerpos a estas inmunoglobulina o componentes complementarios contenidos en antisueros utilizados para ensayos clínicos. Un estricto control del pH, temperatura y concentración de EDTA de una solución a la que se agrega sangre permite la producción selectiva de células C3 o C4 sensibilizadas. La selección de un suero con anticuerpos IgG o IgM y la inactivación de las proteínas indeseadas permite la producción de células IgG o igM sensibilizadas. Las células resultantes tienen un poder de reacción de aglutinación de 2-4+ aproximadamente, Los eritrocitos del hombre y de otros animales pueden experimentar la sensibilización mono-específica. Otras proteínas humanas, como IgA o C5 , y proteínas de plasma de otros animales pueden ser análogamente combinadas mono-específicamente a los glóbulos rojos de la sangre
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/890,558 US4247536A (en) | 1978-03-20 | 1978-03-20 | Method of preparing C3-sensitized erythrocytes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES478806A1 true ES478806A1 (es) | 1979-12-01 |
Family
ID=25396832
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES478806A Expired ES478806A1 (es) | 1978-03-20 | 1979-03-20 | Un metodo de preparacion de eritrocitos c3-c4-sensibilizados. |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4247536A (es) |
| EP (1) | EP0014214A4 (es) |
| CA (1) | CA1114743A (es) |
| ES (1) | ES478806A1 (es) |
| WO (1) | WO1979000796A1 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1980002459A1 (en) * | 1979-04-30 | 1980-11-13 | Immunology Dev Corp | New and improved methodology for the immunodiagnosis of multiple sclerosis and/or malignant diseases from blood sample analysis |
| EP0039195B1 (en) * | 1980-04-28 | 1986-06-18 | Montefiore Hospital and Medical Center | Antibody detection process |
| US4375459A (en) * | 1980-06-20 | 1983-03-01 | The Regents Of The University Of California | Cytoreceptor assay |
| US4693967A (en) * | 1981-02-18 | 1987-09-15 | Research Corporation | Monitoring therapy results in body samples of receptor cells |
| FR2687788B1 (fr) * | 1992-02-24 | 1994-12-30 | Aetsrn | Materiel pour la mise en evidence en phase solide, de complexes antigenes-anticorps par des hematies indicatrices sa methode de preparation et son utilisation. |
| FR2899337B1 (fr) * | 2006-03-31 | 2008-07-04 | Univ Reims Champagne Ardenne | Standard c4d / c4b pour cytometrie de flux quantitative du rejet humoral de transplantation. |
| CN115267181A (zh) * | 2022-07-26 | 2022-11-01 | 广州市第一人民医院(广州消化疾病中心、广州医科大学附属市一人民医院、华南理工大学附属第二医院) | 一种用于冷凝集血常规标本检测的试剂及其检测方法和应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3808189A (en) * | 1973-03-15 | 1974-04-30 | American Cyanamid Co | Isolation of gamma globulin preparations enriched in iga and igm using polyethylene glycol |
| US4130634A (en) * | 1974-03-15 | 1978-12-19 | University Of Illinois Foundation | Method for detecting and quantifying antigens |
| US4092114A (en) * | 1976-10-20 | 1978-05-30 | Fisher Scientific Company | Indirect latex test for determination of immunoglobulins |
-
1978
- 1978-03-20 US US05/890,558 patent/US4247536A/en not_active Expired - Lifetime
-
1979
- 1979-02-28 WO PCT/US1979/000121 patent/WO1979000796A1/en not_active Ceased
- 1979-03-12 CA CA323,176A patent/CA1114743A/en not_active Expired
- 1979-03-20 ES ES478806A patent/ES478806A1/es not_active Expired
- 1979-10-23 EP EP19790901191 patent/EP0014214A4/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP0014214A1 (en) | 1980-08-20 |
| EP0014214A4 (en) | 1980-09-29 |
| WO1979000796A1 (en) | 1979-10-18 |
| US4247536A (en) | 1981-01-27 |
| CA1114743A (en) | 1981-12-22 |
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