ES482205A1 - Metodo de medir colesterol del suero y perfeccionamientos en las disposiciones para su realizacion. - Google Patents
Metodo de medir colesterol del suero y perfeccionamientos en las disposiciones para su realizacion.Info
- Publication number
- ES482205A1 ES482205A1 ES482205A ES482205A ES482205A1 ES 482205 A1 ES482205 A1 ES 482205A1 ES 482205 A ES482205 A ES 482205A ES 482205 A ES482205 A ES 482205A ES 482205 A1 ES482205 A1 ES 482205A1
- Authority
- ES
- Spain
- Prior art keywords
- measuring
- reagents
- serum
- cholesterol content
- fractions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title abstract 6
- 235000012000 cholesterol Nutrition 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 210000002966 serum Anatomy 0.000 title 1
- 102000004895 Lipoproteins Human genes 0.000 abstract 2
- 108090001030 Lipoproteins Proteins 0.000 abstract 2
- 102000004856 Lectins Human genes 0.000 abstract 1
- 108090001090 Lectins Proteins 0.000 abstract 1
- 239000002523 lectin Substances 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/04—Endocrine or metabolic disorders
- G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S210/00—Liquid purification or separation
- Y10S210/902—Materials removed
- Y10S210/903—Nitrogenous
- Y10S210/905—Protein
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Endocrinology (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Método de medir colesterol del suero, que comprende la separación de las fracciones de lipoproteinas según su apoproteina y luego la determinación del contenido de colesterol de por lo menos una de estas fracciones, caracterizado porque la separación se efectúa a partir de una muestra de suero por precipitación selectiva de las fracciones LDL y VLDL que contienen la apoproteina beta por medio de una lectina capaz de formar complejos con ambas lipoproteinas.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB7828815 | 1978-07-04 | ||
| GB7902255 | 1979-01-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES482205A1 true ES482205A1 (es) | 1980-02-16 |
Family
ID=26268119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES482205A Expired ES482205A1 (es) | 1978-07-04 | 1979-07-04 | Metodo de medir colesterol del suero y perfeccionamientos en las disposiciones para su realizacion. |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US4366244A (es) |
| EP (1) | EP0006822B1 (es) |
| DE (1) | DE2963844D1 (es) |
| ES (1) | ES482205A1 (es) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4273867A (en) * | 1979-04-05 | 1981-06-16 | Mallinckrodt, Inc. | Method and reagent for counteracting lipemic interference |
| FR2513766B1 (fr) * | 1981-09-28 | 1985-09-13 | Biomerieux Sa | Procedes et reactifs de separation selective des lipoproteines de faible densite ldl et de quantification de leurs composants |
| DE3533288A1 (de) * | 1985-09-18 | 1987-03-26 | Boehringer Mannheim Gmbh | Verfahren und reagenz zur spezifischen bestimmung von hdl-cholesterin im serum |
| US5135716A (en) * | 1989-07-12 | 1992-08-04 | Kingston Diagnostics, L.P. | Direct measurement of HDL cholesterol via dry chemistry strips |
| US5783400A (en) * | 1990-04-27 | 1998-07-21 | Genzyme Corporation | Method for the isolation of lipoprotein allowing for the subsequent quantification of its mass and cholesterol content |
| WO1992021015A1 (en) * | 1991-05-23 | 1992-11-26 | Seman Leo J Jr | METHOD FOR DETECTING LIPOPROTEIN (a) AND ASSOCIATED CHOLESTEROL |
| US5286626A (en) * | 1991-12-13 | 1994-02-15 | Actimed Laboratories, Inc. | Process and apparatus for direct determination of low density lipoprotein |
| US5344571A (en) * | 1992-02-18 | 1994-09-06 | University Of Texas System Board Of Regents | Method for separating isoanalytes and measuring analytes in fluids |
| AU4924193A (en) * | 1992-09-24 | 1994-04-12 | Perseptive Biosystems, Inc. | Quantitative measurement of ldl |
| US5460974A (en) * | 1992-10-13 | 1995-10-24 | Miles Inc. | Method of assaying whole blood for HDL cholesterol |
| US5618683A (en) * | 1993-04-13 | 1997-04-08 | Brocia; Robert W. | Diagnostic kit for cholesteryl ester transfer protein (CETP) activity measurement and a new synthetic particle used therein |
| US5585235A (en) * | 1993-04-13 | 1996-12-17 | Diagnescent Technologies, Inc. | Fluorescent assay and method that corrects for spectral interference |
| US5766552A (en) * | 1993-04-20 | 1998-06-16 | Actimed Laboratories, Inc. | Apparatus for red blood cell separation |
| US5660798A (en) * | 1993-04-20 | 1997-08-26 | Actimed Laboratories, Inc. | Apparatus for red blood cell separation |
| KR980010429A (ko) * | 1996-07-18 | 1998-04-30 | 다나까 모또아끼 | 콜레스테롤 측량용 시약 |
| DE60207196T2 (de) | 2002-04-09 | 2006-07-20 | Cholestech Corp., Hayward | Verfahren und Vorrichtung zur Quantifizierung von Lipoprotein-Cholesterol hoher Dichte |
| DE10258134B4 (de) * | 2002-11-29 | 2006-02-09 | Universität Potsdam | Verfahren zum Nachweis, zur Differenzierung und/oder zur Anreicherung von Stoffen mittels Ausfällung |
| EP2126587B1 (en) | 2007-01-09 | 2010-09-29 | Cholestech Corporation | Device and method for measuring ldl-associated cholesterol |
| CA3054541C (en) * | 2007-06-08 | 2022-11-29 | Quest Diagnostics Investments Incorporated | Lipoprotein analysis by differential charged-particle mobility |
| GB0711236D0 (en) * | 2007-06-11 | 2007-07-18 | Oxford Biosensors Ltd | Lipoprotein surfactant |
| CN112941144B (zh) * | 2021-03-01 | 2022-02-15 | 江西英大生物技术有限公司 | 小而密低密度脂蛋白胆固醇测定试剂盒 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL35169A (en) * | 1970-08-26 | 1974-11-29 | Yeda Res & Dev | Process for preparing insolubilized crystalline concanavalin a |
| US3839314A (en) * | 1971-06-29 | 1974-10-01 | Baxter Laboratories Inc | Clarification of blood serum and plasma using block copolymers of ethylene oxide and polyoxypropylene |
| US3902964A (en) * | 1971-12-13 | 1975-09-02 | U S Medical Research And Dev I | Method of and apparatus for chemically separating plasma or serum from formed elements of blood |
| US3884638A (en) * | 1973-08-01 | 1975-05-20 | Damon Corp | Method of determining cholesterol |
| US3884764A (en) * | 1974-03-25 | 1975-05-20 | Eastman Kodak Co | Method and composition for blood serum cholesterol analysis |
| US4148869A (en) * | 1975-03-06 | 1979-04-10 | Baxter Travenol Laboratories, Inc. | Immunological reagent and method of using same |
| US3955125A (en) * | 1975-02-21 | 1976-05-04 | General Signal Corporation | Fail-safe active timing circuit |
| US4045176A (en) * | 1975-06-13 | 1977-08-30 | Proksch Gary J | Preparation of optically clear serum |
| US4039285A (en) * | 1975-10-10 | 1977-08-02 | Ortho Diagnostics, Inc. | Single sample method for determination of lipoprotein concentrations in blood |
| DE2600664C3 (de) * | 1976-01-09 | 1980-10-23 | Claus-Christian Dr.Rer.Nat. Dr.Med. 6901 Wilhelmsfeld Heuck | Verfahren zur direkten Bestimmung des ß -Cholesteringehaltes im Blutserum |
| NZ180199A (en) * | 1976-03-04 | 1978-11-13 | New Zealand Dev Finance | Method of testing for the presence of elevated plasma liprotein concentration |
| US4110077A (en) * | 1976-10-08 | 1978-08-29 | Hoffmann-La Roche Inc. | Determination of β-lipoproteins in blood serum with polyanethole sulfonate |
| US4126416A (en) * | 1977-11-21 | 1978-11-21 | The Trustees Of Boston University | Method for determining the level of LDL cholesterol in blood plasma |
| US4190628A (en) * | 1977-11-21 | 1980-02-26 | Trustees Of Boston University | Kit for determining the level of LDL cholesterol in body fluids |
| US4188188A (en) * | 1978-09-27 | 1980-02-12 | Bio-Rad Laboratories, Inc. | High density lipoprotein cholesterol assay |
-
1979
- 1979-07-02 EP EP79400450A patent/EP0006822B1/fr not_active Expired
- 1979-07-02 DE DE7979400450T patent/DE2963844D1/de not_active Expired
- 1979-07-04 ES ES482205A patent/ES482205A1/es not_active Expired
-
1981
- 1981-03-16 US US06/243,690 patent/US4366244A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0006822B1 (fr) | 1982-10-13 |
| US4366244A (en) | 1982-12-28 |
| DE2963844D1 (en) | 1982-11-18 |
| EP0006822A1 (fr) | 1980-01-09 |
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