ES483376A1 - Procedimiento para la obtencion de un preparado de enzimas inmovilizadas. - Google Patents

Procedimiento para la obtencion de un preparado de enzimas inmovilizadas.

Info

Publication number
ES483376A1
ES483376A1 ES483376A ES483376A ES483376A1 ES 483376 A1 ES483376 A1 ES 483376A1 ES 483376 A ES483376 A ES 483376A ES 483376 A ES483376 A ES 483376A ES 483376 A1 ES483376 A1 ES 483376A1
Authority
ES
Spain
Prior art keywords
particles
hydrocarbon
dispersion
liquid hydrocarbon
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES483376A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Imperial Chemical Industries Ltd
Original Assignee
Imperial Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial Chemical Industries Ltd filed Critical Imperial Chemical Industries Ltd
Publication of ES483376A1 publication Critical patent/ES483376A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/091—Phenol resins; Amino resins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Procedimiento para la obtención de un preparado de enzimas inmovilizadas en donde las moléculas de por lo menos una enzima se adhieren a grupos amínicos sobre la superficie de material de soporte en partículas, comprendiendo el material de soporte partículas que tienen grupos amínicos en sus superficies; caracterizado porque comprende las etapas siguientes: (1) producir una dispersión de partículas que tienen grupos amínicos en sus superficies mediante un proceso de preparación de dispersiones para la preparación de una dispersión estable en un hidrocarburo líquido, por polimerización en dicho hidrocarburo líquido de uno o más reactivos formadores de poliamidas; siendo por lo menos uno de dichos reactivos insoluble en el hidrocarburo líquido a la temperatura de polimerización, y constando el proceso de preparación de dispersiones de los siguientes pasos: A. Llevar dicho(s) reactivo(s) insoluble(s) a un estado de sispresión estable de partículas finamente divididas en el hidrocarburo líquido con ayuda de un medio dispersante soluble en el hidrocarburo y que incorpora en la molécula (a) un componente polimérico que es solvatizable por el hidrocarburo y (b) un agrupamiento que es capaz de asociarse con las partículas del (de los) reactante(s) insoluble(s), y B. Calentar la dispersión de reactivo(s) insoluble(s), junto con cualquiera otros reactivos solubles en el hidrocarburo líquido que sean necesarios, a la temperatura de polimerización en presencia de un medio estabilizador de polímeros solubles en el hidrocarburo y que incorpore en la molécula (i) un componente polimérico solvatizable por el hidrocarburo y (ii) un agrupamiento capaz de asociarse con las partículas de la poliamida resultante a medida que se forma ese polímero; (2) separar las partículas de la dispersión; y (3) poner en contacto las partículas con una enzima, por lo menos, por el tiempo y bajo las condiciones necesarias para que las moléculas de la enzima se adhieran a grupos amínicos sobre las superficiesde las partículas.
ES483376A 1978-08-15 1979-08-14 Procedimiento para la obtencion de un preparado de enzimas inmovilizadas. Expired ES483376A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB7833374 1978-08-15

Publications (1)

Publication Number Publication Date
ES483376A1 true ES483376A1 (es) 1980-09-01

Family

ID=10499060

Family Applications (1)

Application Number Title Priority Date Filing Date
ES483376A Expired ES483376A1 (es) 1978-08-15 1979-08-14 Procedimiento para la obtencion de un preparado de enzimas inmovilizadas.

Country Status (7)

Country Link
EP (1) EP0008498A1 (es)
JP (1) JPS5526900A (es)
BR (1) BR7905209A (es)
DK (1) DK330779A (es)
ES (1) ES483376A1 (es)
RO (1) RO77085A (es)
YU (1) YU198479A (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0164889A3 (en) * 1984-05-07 1986-12-17 HSC Research Development Corporation Surface-treated polymeric substrates
GB8701706D0 (en) * 1987-01-27 1987-03-04 Ici Plc Immobilisation of enzymes
CN105996061A (zh) * 2016-05-12 2016-10-12 河南泰利杰生物科技有限公司 一种难消化糊精的制备工艺
CN113512153B (zh) * 2021-04-15 2022-06-10 陕西科技大学 一种ucst型温敏性聚合物固定化酶的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2450132A1 (de) * 1974-10-22 1976-04-29 Ici Ltd Traegerenzym
NL185520C (es) * 1976-02-19 Ici Ltd

Also Published As

Publication number Publication date
JPS5526900A (en) 1980-02-26
BR7905209A (pt) 1980-07-29
EP0008498A1 (en) 1980-03-05
RO77085A (ro) 1981-06-22
YU198479A (en) 1982-10-31
DK330779A (da) 1980-02-16

Similar Documents

Publication Publication Date Title
Prucker et al. Surface-attached hydrogel coatings via C, H-insertion crosslinking for biomedical and bioanalytical applications
Oishi et al. Dynamically programmed switchable DNA hydrogels based on a DNA circuit mechanism
DE60027309D1 (de) Polymerverzweigungen zur immobilisierung von molekülen auf oberflächen oder substraten, wobei die polymere wasserlösliche oder wasserdispergierbare segmente und sonden aufweisen
US6602692B1 (en) Method for immobilizing biomolecules and affinity ligands on polymer carriers
ATE48018T1 (de) Hybridisierungstest fuer nukleinsaeure.
Lilge et al. Block copolymer brushes for completely decoupled control of determinants of cell–surface interactions
BR8402533A (pt) Polimero,processo para sua preparacao,e sua aplicacao
ES2132392T3 (es) Inmovilizacion de reactivos de ensayo de enlace especifico.
Kobel et al. Micropatterning of hydrogels by soft embossing
NO912565D0 (no) Fremgangsmaate til fremstilling av fortynnede opploesningerav hoeymolekylaere polymerer.
ATE508793T1 (de) Herstellung und verwendung von substraten
JP2021506319A (ja) ヒドロゲル型の生体機能チップマイクロ流体デバイス
Wancura et al. Fabrication, chemical modification, and topographical patterning of reactive gels assembled from azlactone‐functionalized polymers and a diamine
Osaki et al. Material adhesion through direct covalent bond formation assisted by noncovalent interactions
DK256088D0 (da) Polymerpartikler med implanterede amfiphile molekyler og fremgangsmaade til fremstilling af saadanne partikler
SE7907035L (sv) Forfarande for framstellning av flytande brensle ur biologiska ravaror
Tian et al. Engineering tough supramolecular hydrogels with structured micropillars for tunable wetting and adhesion properties
Heffernan et al. Temperature responsive hydrogels enable transient three‐dimensional tumor cultures via rapid cell recovery
ES2125986T3 (es) Sensor para ensayo optico.
EP0351950A3 (en) Use of plasma to immobilize protein on polymeric surfaces
Paschke et al. Poly (oxanorbornene)‐based polyzwitterions with systematically increasing hydrophobicity: synthesis, physical characterization, and biological properties
ATE135745T1 (de) Immobilisierung von organischen makromolekülen oder biopolymeren in einer polymermembran
Jun et al. Preparation of biomimetic hydrogels with controlled cell adhesive properties and topographical features for the study of muscle cell adhesion and proliferation
EP0366241A3 (en) Device with adsorbent surface and method
Kühlmeyer et al. Stabilisation of enzymes with polyvinylsaccharides I: physical stabilisation of horseradish peroxidase