ES8101618A1 - Un metodo para la manufactura de un producto enzimatico mi- crobiano de b-galacto sidasa. - Google Patents

Un metodo para la manufactura de un producto enzimatico mi- crobiano de b-galacto sidasa.

Info

Publication number
ES8101618A1
ES8101618A1 ES487503A ES487503A ES8101618A1 ES 8101618 A1 ES8101618 A1 ES 8101618A1 ES 487503 A ES487503 A ES 487503A ES 487503 A ES487503 A ES 487503A ES 8101618 A1 ES8101618 A1 ES 8101618A1
Authority
ES
Spain
Prior art keywords
galactosidase
beta
lactose
production
hydrolysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES487503A
Other languages
English (en)
Other versions
ES487503A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Industri AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK10379A external-priority patent/DK10379A/da
Application filed by Novo Industri AS filed Critical Novo Industri AS
Publication of ES487503A0 publication Critical patent/ES487503A0/es
Publication of ES8101618A1 publication Critical patent/ES8101618A1/es
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C21/00—Whey; Whey preparations
    • A23C21/02—Whey; Whey preparations containing, or treated with, microorganisms or enzymes
    • A23C21/023—Lactose hydrolysing enzymes, e.g. lactase, B-galactosidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO DE OBTENCION DE UNA BETA-GALACTOSIDASA. CONSTA DE LAS SIGUIENTES ETAPAS: 1) CULTIVO, EN UN MEDIO DE FERMENTACION, CON FUENTES DE NITROGENO, CARBONO Y OXIGENO, ASIMILABLES; UN MICROORGANISMO PERTENECIENTE AL GRUPO TAXONOMICA, REPRESENTADO POR EL CULTIVO TIPO NRRL B-11, 229. 2) RECOGIDA DE LAS CELULAS. 3) AISLADO DEL PRODUCTO INTRACELULAR DE BETA-GALACTOSIDASA, INMOVILIZADO. EL PRODUCTO SE OBTIENE INTRACELULARMENTE, PERO SE PUEDE DESPRENDER DE LAS CELULAS POR TRATAMIENTO CON TOLUENO, BUTANOL O LISOZIMA, TRATAMIENTO ULTRASONICO O DESINTEGRACION MECANICA, DESPUES DE LO CUAL SE SEPARA LOS RESIDUOS CELULARES Y SE PURIFICA LA ENZIMA, POR EJEMPLO, POR PRECIPITACION SALINA O CON UN DISOLVENTE ORGANICO.
ES487503A 1979-01-10 1980-01-07 Un metodo para la manufactura de un producto enzimatico mi- crobiano de b-galacto sidasa. Expired ES8101618A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK10379A DK10379A (da) 1979-01-10 1979-01-10 Mikrobiel beta-galactosidase mikroorganisme til fremstilling deraf fremgangsmaade til fremstilling deraf og anvendelse deraf
DK519079A DK146325C (da) 1979-01-10 1979-12-06 Beta-galactosidase, fremgangsmaade til fremstilling deraf og anvendelse deraf

Publications (2)

Publication Number Publication Date
ES487503A0 ES487503A0 (es) 1980-12-16
ES8101618A1 true ES8101618A1 (es) 1980-12-16

Family

ID=26063306

Family Applications (1)

Application Number Title Priority Date Filing Date
ES487503A Expired ES8101618A1 (es) 1979-01-10 1980-01-07 Un metodo para la manufactura de un producto enzimatico mi- crobiano de b-galacto sidasa.

Country Status (9)

Country Link
AU (1) AU5438480A (es)
DE (1) DE3000598A1 (es)
DK (1) DK146325C (es)
ES (1) ES8101618A1 (es)
FR (1) FR2453214A1 (es)
GB (1) GB2038837A (es)
IT (1) IT8047556A0 (es)
NL (1) NL8000033A (es)
NZ (1) NZ192552A (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3811964A1 (de) * 1988-04-11 1989-10-19 Biodyn Ag Getraenk fuer menschliche ernaehrung
FR2749307B1 (fr) * 1996-05-29 1998-09-04 Roquette Freres Procede de preparation de d-arabitol

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1493542A (en) * 1974-12-04 1977-11-30 Reynolds Tobacco Co R Production of thermostable lactose
NZ190603A (en) * 1978-06-07 1982-03-23 Nat Res Dev Heat-stable -galactosidase derived from bacillus stearothermophilus hydrolysis of lactose

Also Published As

Publication number Publication date
NL8000033A (nl) 1980-07-14
DK519079A (da) 1980-07-11
DK146325C (da) 1984-02-20
DE3000598A1 (de) 1980-07-31
ES487503A0 (es) 1980-12-16
NZ192552A (en) 1982-03-30
FR2453214A1 (fr) 1980-10-31
AU5438480A (en) 1980-07-17
DK146325B (da) 1983-09-05
GB2038837A (en) 1980-07-30
IT8047556A0 (it) 1980-01-09

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19971001