ES8205465A1 - Procedimiento para la fijacion de un antigeno fijado especi-ficamente a un primer anticuerpo - Google Patents

Procedimiento para la fijacion de un antigeno fijado especi-ficamente a un primer anticuerpo

Info

Publication number
ES8205465A1
ES8205465A1 ES503763A ES503763A ES8205465A1 ES 8205465 A1 ES8205465 A1 ES 8205465A1 ES 503763 A ES503763 A ES 503763A ES 503763 A ES503763 A ES 503763A ES 8205465 A1 ES8205465 A1 ES 8205465A1
Authority
ES
Spain
Prior art keywords
antibody
fixed
antigen
procedure
attaching
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES503763A
Other languages
English (en)
Other versions
ES503763A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES8205465A1 publication Critical patent/ES8205465A1/es
Publication of ES503763A0 publication Critical patent/ES503763A0/es
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
    • G01N33/541—Double or second antibody, i.e. precipitating antibody

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Steroid Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Measurement Of Radiation (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Adhesive Tapes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA LA FIJACION DE UN ANTIGENO FIJADO ESPECIFICAMENTE A UN PRIMER ANTICUERPO, EN UNA DISOLUCION ACUOSA, Y PARA LA SEPARACION DEL ANTIGENO FIJADO RESPECTO DEL ANTIGENO NO FIJADO, QUE TIENE APLICACIONES PARA LA DETERMINACION DE LA CONCENTRACION DE ANTIGENO EN UN LIQUIDO CORPORAL EN RADIOINMUNOANALISIS COMPETITIVO. CONSISTE EN SEPARAR DEL ANTIGENO LIBRE EL ANTICUERPO CON EL ANTIGENO FIJADO ESPECIFICAMENTE POR MWEDIO DE UN SEGUNDO ANTOCUERPO, QUE ESTA FIJADO POR UNA PARTE A UNA CELULA DE "STAPHYLOCOCCUS AUREAUS" Y POR OTRA ESPECIFICAMENTE AL PRIMER ANTICUERPO. LA SEPARACION SE LLEVA A CABO AÑADIENDO A LA DISOLUCION ACUOSA DEL ANTICUERPO UN ACELERADOR DE REACCION, EN MEDIO TAMPONADO CON FOSFATO SODICO.
ES503763A 1980-07-14 1981-07-08 Procedimiento para la fijacion de un antigeno fijado especi-ficamente a un primer anticuerpo Granted ES503763A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19803026665 DE3026665A1 (de) 1980-07-14 1980-07-14 Verfahren zum binden und trennen fuer den kompetitiven radioimmunoassay und reagenz hierzu

Publications (2)

Publication Number Publication Date
ES8205465A1 true ES8205465A1 (es) 1982-06-01
ES503763A0 ES503763A0 (es) 1982-06-01

Family

ID=6107171

Family Applications (1)

Application Number Title Priority Date Filing Date
ES503763A Granted ES503763A0 (es) 1980-07-14 1981-07-08 Procedimiento para la fijacion de un antigeno fijado especi-ficamente a un primer anticuerpo

Country Status (15)

Country Link
EP (1) EP0044041B1 (es)
JP (1) JPS5749861A (es)
AT (1) ATE12316T1 (es)
AU (1) AU7281281A (es)
CA (1) CA1172959A (es)
DD (1) DD202073A5 (es)
DE (2) DE3026665A1 (es)
DK (1) DK310781A (es)
ES (1) ES503763A0 (es)
FI (1) FI812187L (es)
IL (1) IL63291A0 (es)
NO (1) NO812397L (es)
PL (1) PL232148A1 (es)
PT (1) PT73359B (es)
ZA (1) ZA814741B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU647428B2 (en) * 1989-12-01 1994-03-24 Dade Behring Marburg Gmbh Immunoassay for antibodies to infectious disease agents
DE4202924A1 (de) * 1992-02-01 1993-08-05 Behringwerke Ag Verfahren zur bestimmung von rheumafaktoren und mittel zur durchfuehrung des verfahrens

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3981982A (en) * 1974-09-11 1976-09-21 Abbott Laboratories Radioimmunoassay for determining the digoxin content of a sample
US3995019A (en) * 1975-03-04 1976-11-30 Baxter Travenol Laboratories, Inc. Diagnostic reagent system
US4018883A (en) * 1975-03-10 1977-04-19 Nichols Institute For Endocrinology Thyroxine (T4) radioimmunoassay
US4139604A (en) * 1977-09-16 1979-02-13 Becton, Dickinson And Company Separation technique for assays
FI792576A7 (fi) * 1979-08-20 1981-01-01 Orion Yhtymae Oy Parannettu entsyymi-immunologinen määritysmenetelmä.

Also Published As

Publication number Publication date
JPS5749861A (en) 1982-03-24
ATE12316T1 (de) 1985-04-15
DD202073A5 (de) 1983-08-24
ZA814741B (en) 1982-07-28
DE3026665A1 (de) 1982-02-11
EP0044041B1 (de) 1985-03-20
IL63291A0 (en) 1981-10-30
CA1172959A (en) 1984-08-21
PT73359A (en) 1981-08-01
DK310781A (da) 1982-01-15
EP0044041A1 (de) 1982-01-20
PL232148A1 (es) 1982-03-15
PT73359B (de) 1983-01-13
DE3169371D1 (en) 1985-04-25
FI812187A7 (fi) 1982-01-15
FI812187L (fi) 1982-01-15
NO812397L (no) 1982-01-15
AU7281281A (en) 1982-01-21
ES503763A0 (es) 1982-06-01

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