ES8501444A1 - Un metodo para la determinacion cuantitativa de bilirrubina conjugada en fluidos biologicos. - Google Patents
Un metodo para la determinacion cuantitativa de bilirrubina conjugada en fluidos biologicos.Info
- Publication number
- ES8501444A1 ES8501444A1 ES524270A ES524270A ES8501444A1 ES 8501444 A1 ES8501444 A1 ES 8501444A1 ES 524270 A ES524270 A ES 524270A ES 524270 A ES524270 A ES 524270A ES 8501444 A1 ES8501444 A1 ES 8501444A1
- Authority
- ES
- Spain
- Prior art keywords
- bilirubin
- oxidase
- conjugated
- concn
- surfactant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 title abstract 14
- 108010015428 Bilirubin oxidase Proteins 0.000 title abstract 4
- 108010029541 Laccase Proteins 0.000 title abstract 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 title abstract 2
- 239000003814 drug Substances 0.000 title abstract 2
- 229940079593 drug Drugs 0.000 title abstract 2
- 239000004094 surface-active agent Substances 0.000 title abstract 2
- 101100295848 Drosophila melanogaster Optix gene Proteins 0.000 title 1
- 108091005804 Peptidases Proteins 0.000 title 1
- 239000004365 Protease Substances 0.000 title 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title 1
- 101150001027 Suco gene Proteins 0.000 title 1
- 238000010521 absorption reaction Methods 0.000 abstract 3
- 239000013060 biological fluid Substances 0.000 abstract 3
- 206010023126 Jaundice Diseases 0.000 abstract 2
- 241000223251 Myrothecium Species 0.000 abstract 2
- 238000011088 calibration curve Methods 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 2
- BPYKTIZUTYGOLE-UHFFFAOYSA-N billirubin-IXalpha Natural products N1C(=O)C(C)=C(C=C)C1=CC1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(C=C3C(=C(C=C)C(=O)N3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-UHFFFAOYSA-N 0.000 abstract 1
- 238000003759 clinical diagnosis Methods 0.000 abstract 1
- 230000003902 lesion Effects 0.000 abstract 1
- 210000005229 liver cell Anatomy 0.000 abstract 1
- 208000019423 liver disease Diseases 0.000 abstract 1
- 230000005976 liver dysfunction Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- -1 sulpho Chemical class 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METODO PARA LA DETERMINACION CUANTITATIVA DE BILIRRUBINA EN FLUIDOS BIOLOGICOS.CONSISTE EN: A) HACER REACCIONAR UNA SERIE DE SOLUCIONES ACUOSAS DE BILIRRUBINA DE CONCENTRACIONES VARIABLES CON UNA ENZIMA OXIDANTE DE BILIRRUBINA (BILIRRUBINA OXIDASA O LACASA), PARA CONSTRUIR UNA CURVA DE CALIBRACION QUE REPRESENTA LA REACCION ENTRE LA ABSORBANCIA DE LA MEZCLA DE REACCION Y LA CONCENTRACION DE BILIRRUBINA; B) HACER REACCIONAR UN FLUIDO BIOLOGICO DONDE LA CONCENTRACION DE BILIRRUBINA ES DESCONOCIDA CON DICHA ENZIMA DE OXIDACION DE BILIRRUBINA Y MEDIR LA ABSORBANCIA DE LA MEZCLA DE REACCION RESULTANTE, Y C) CALCULAR LA CONCENTRACION DE BILIRRUBINA EN EL FLUIDO BIOLOGICO POR COMPARACION DEL VALOR MEDIO EN LA ETAPA (B), CON LA CURVA DE CALIBRACION.SE UTILIZA PARA DETERMINAR CUANTITATIVAMENTE EL CONTENIDO DE BILIRRUBINA EN FLUIDOS BIOLOGICOS, COMO POR EJEMPLO EL SUERO SANGUINEO.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12763382A JPS5918000A (ja) | 1982-07-23 | 1982-07-23 | 生体成分の測定法及びそれに用いる試薬組成物 |
| JP12763282A JPS5917999A (ja) | 1982-07-23 | 1982-07-23 | ビリルビンの定量法及びその定量用試薬組成物 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES524270A0 ES524270A0 (es) | 1984-12-01 |
| ES8501444A1 true ES8501444A1 (es) | 1984-12-01 |
Family
ID=26463537
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES524270A Expired ES8501444A1 (es) | 1982-07-23 | 1983-07-19 | Un metodo para la determinacion cuantitativa de bilirrubina conjugada en fluidos biologicos. |
| ES534138A Expired ES8601482A1 (es) | 1982-07-23 | 1984-07-09 | Un metodo para la determinacion cuantitativa de componentes fisiologicos en fluidos biologicos. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES534138A Expired ES8601482A1 (es) | 1982-07-23 | 1984-07-09 | Un metodo para la determinacion cuantitativa de componentes fisiologicos en fluidos biologicos. |
Country Status (2)
| Country | Link |
|---|---|
| ES (2) | ES8501444A1 (es) |
| IT (1) | IT1203658B (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111424070B (zh) * | 2020-03-03 | 2023-01-20 | 天津大学 | 含有枯草芽孢杆菌漆酶的总胆红素检测试剂盒 |
-
1983
- 1983-06-21 IT IT48544/83A patent/IT1203658B/it active
- 1983-07-19 ES ES524270A patent/ES8501444A1/es not_active Expired
-
1984
- 1984-07-09 ES ES534138A patent/ES8601482A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| ES524270A0 (es) | 1984-12-01 |
| ES534138A0 (es) | 1985-10-16 |
| IT8348544A0 (it) | 1983-06-21 |
| IT1203658B (it) | 1989-02-15 |
| ES8601482A1 (es) | 1985-10-16 |
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