ES8503955A1 - Procedimiento para separar y purificar interferon por cromatografia - Google Patents

Procedimiento para separar y purificar interferon por cromatografia

Info

Publication number
ES8503955A1
ES8503955A1 ES534686A ES534686A ES8503955A1 ES 8503955 A1 ES8503955 A1 ES 8503955A1 ES 534686 A ES534686 A ES 534686A ES 534686 A ES534686 A ES 534686A ES 8503955 A1 ES8503955 A1 ES 8503955A1
Authority
ES
Spain
Prior art keywords
interferon
chromatography
silicone gel
mixture
isolating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES534686A
Other languages
English (en)
Other versions
ES534686A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES534686A0 publication Critical patent/ES534686A0/es
Publication of ES8503955A1 publication Critical patent/ES8503955A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA LA SEPARACION Y PURIFICACION DE INTERFERON POR CROMATOGRAFIA.EL INTERFERON A SEPARAR O PURIFICAR ES CROMATOGRAFIADO CON UNA MEZCLA DE AGENTES ELUYENTES ORGANICOS EN GEL DE SILICE. UNA MEZCLA PREFERENTE DE ELUYENTES ES, EN PARTES VOLUMETRICAS, DE 300/800 PARA EL CLOROFORMO O CLORURO DE METILENO; 400/700 DE METANOL; 100/300 DE AGUA; 0/150 DE ETILENGLICOLMONOMETILETER; 1/15 DE ACIDO ACETICO; 3/8 DE TRIELANINA; Y 1/10 DE ACETATO AMONICO. LOS MATERIALES DE PARTIDA SON, ESENCIALMENTE, CULTIVOS DE BACTERIAS O LEVADURAS GENETICAMENTE MODIFICADAS Y PORCIONES SOBRENADANTES DE CULTIVOS DE CELULAS INDUCIDAS DE ANIMALES MAMIFEROS. EL GEL DE SILICE ES EL HABITUAL PARA CROMATOGRAFIA, CON GRANOS ENTRE 0,035 Y 0,10 MM.
ES534686A 1983-07-29 1984-07-27 Procedimiento para separar y purificar interferon por cromatografia Expired ES8503955A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19833327710 DE3327710A1 (de) 1983-07-29 1983-07-29 Verfahren zur isolierung und reinigung von interferon

Publications (2)

Publication Number Publication Date
ES534686A0 ES534686A0 (es) 1985-04-01
ES8503955A1 true ES8503955A1 (es) 1985-04-01

Family

ID=6205464

Family Applications (1)

Application Number Title Priority Date Filing Date
ES534686A Expired ES8503955A1 (es) 1983-07-29 1984-07-27 Procedimiento para separar y purificar interferon por cromatografia

Country Status (14)

Country Link
EP (1) EP0133284A2 (es)
JP (1) JPS6069099A (es)
KR (1) KR850001289A (es)
AU (1) AU3127384A (es)
DE (1) DE3327710A1 (es)
DK (1) DK369084A (es)
ES (1) ES8503955A1 (es)
FI (1) FI842982A7 (es)
GR (1) GR82439B (es)
HU (1) HUT35003A (es)
IL (1) IL72511A0 (es)
NO (1) NO843049L (es)
PT (1) PT78981A (es)
ZA (1) ZA845818B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3500681A1 (de) * 1985-01-11 1986-07-17 Hoechst Ag, 6230 Frankfurt Verfahren zur isolierung und reinigung von lymphokinen
US4675387A (en) * 1985-07-26 1987-06-23 E. I. Du Pont De Nemours And Company Method for extracting protein with organic acid

Also Published As

Publication number Publication date
HUT35003A (en) 1985-05-28
GR82439B (es) 1984-12-13
FI842982L (fi) 1985-01-30
DE3327710A1 (de) 1985-02-07
KR850001289A (ko) 1985-03-18
PT78981A (de) 1984-08-01
ZA845818B (en) 1985-03-27
DK369084D0 (da) 1984-07-27
FI842982A0 (fi) 1984-07-26
FI842982A7 (fi) 1985-01-30
NO843049L (no) 1985-01-30
AU3127384A (en) 1985-01-31
ES534686A0 (es) 1985-04-01
JPS6069099A (ja) 1985-04-19
IL72511A0 (en) 1984-11-30
DK369084A (da) 1985-01-30
EP0133284A2 (de) 1985-02-20

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19971201