ES8506088A1 - Procedimiento de preparacion de un clono bacteriano produciendo 1-antitripsina humana - Google Patents
Procedimiento de preparacion de un clono bacteriano produciendo 1-antitripsina humanaInfo
- Publication number
- ES8506088A1 ES8506088A1 ES529882A ES529882A ES8506088A1 ES 8506088 A1 ES8506088 A1 ES 8506088A1 ES 529882 A ES529882 A ES 529882A ES 529882 A ES529882 A ES 529882A ES 8506088 A1 ES8506088 A1 ES 8506088A1
- Authority
- ES
- Spain
- Prior art keywords
- dna
- contg
- antitrypsin
- hybrids
- double
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000001580 bacterial effect Effects 0.000 title abstract 2
- 102000015395 alpha 1-Antitrypsin Human genes 0.000 title 1
- 108010050122 alpha 1-Antitrypsin Proteins 0.000 title 1
- 229940024142 alpha 1-antitrypsin Drugs 0.000 title 1
- 241000588724 Escherichia coli Species 0.000 abstract 2
- 239000002299 complementary DNA Substances 0.000 abstract 2
- 238000000338 in vitro Methods 0.000 abstract 2
- 108020004999 messenger RNA Proteins 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 206010014561 Emphysema Diseases 0.000 abstract 1
- 101000823116 Homo sapiens Alpha-1-antitrypsin Proteins 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000003344 environmental pollutant Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 102000051631 human SERPINA1 Human genes 0.000 abstract 1
- 210000004185 liver Anatomy 0.000 abstract 1
- 231100000516 lung damage Toxicity 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000013600 plasmid vector Substances 0.000 abstract 1
- 231100000719 pollutant Toxicity 0.000 abstract 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PROCEDIMIENTO DE PREPARACION DE UN CLONO BACTERIANO PRODUCIENDO BAB1-ANTITRIPSINA HUMANA.COMPRENDE: A) EXTRAER Y PURIFICAR LOS ARN MENSAJEROS TOTALES DE HIGADO HUMANO; B) ENRIQUECER ESTOS MENSAJEROS EN MOLECULAS CODIFICANTES PARA LABAB1-ANTITRIPSINA HUMANA; C) LA SINTESIS IN VITRO DEL ADN COMPLEMENTARIO DOBLE HEBRA AL SEPARARSE ESTA FRACCION MENSAJERA ENRIQUECIDA; D) ENRIQUECER LA PREPARACION DE ADN COMPLEMENTARIO DOBLE HEBRA EN MOLECULAS DE TAMAN/O CORRESPONDIENTE AL DEL ADN-AT; E) ADICIONAR EXTREMIDADES COHEISVAS AL ADN-AT; F) MEZCLAR IN VITRO LA FRACCION ENRIQUECIDA CON UN VECTOR PLASMIDA; G) TRANSFORMAR LAS BACTERIAS HUESPEDES ESCHERICHIA COLI POR LAS MOLECULAS HIBRIDAS; H) SELECCIONAR, ENTRE ESTAS BACTERIAS, PARA OBTENER UN BANCO DE CONOS; I) AISLAR DE ESTE BANCO LOS CLONOS PORTADORES DEL ADN-AT; Y J) IDENTIFICAR DE ENTRE LOS CLONOS LOS QUE EXPRESAN LA BAB1-ANTITRIPSINA HUMANA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BE895961 | 1983-02-21 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES8506088A1 true ES8506088A1 (es) | 1985-07-01 |
| ES529882A0 ES529882A0 (es) | 1985-07-01 |
Family
ID=3862060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES529882A Granted ES529882A0 (es) | 1983-02-21 | 1984-02-20 | Procedimiento de preparacion de un clono bacteriano produciendo 1-antitripsina humana |
Country Status (4)
| Country | Link |
|---|---|
| ES (1) | ES529882A0 (es) |
| GR (1) | GR79803B (es) |
| PT (1) | PT78130B (es) |
| ZA (1) | ZA841033B (es) |
-
1984
- 1984-02-13 ZA ZA841033A patent/ZA841033B/xx unknown
- 1984-02-20 ES ES529882A patent/ES529882A0/es active Granted
- 1984-02-20 GR GR73853A patent/GR79803B/el unknown
- 1984-02-20 PT PT7813084A patent/PT78130B/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PT78130B (fr) | 1986-05-19 |
| ZA841033B (en) | 1984-09-26 |
| GR79803B (es) | 1984-10-31 |
| ES529882A0 (es) | 1985-07-01 |
| PT78130A (fr) | 1984-03-01 |
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