ES8603952A1 - Un metodo de aislar mutantes por supresion de vibrio cholerae - Google Patents

Un metodo de aislar mutantes por supresion de vibrio cholerae

Info

Publication number
ES8603952A1
ES8603952A1 ES530249A ES530249A ES8603952A1 ES 8603952 A1 ES8603952 A1 ES 8603952A1 ES 530249 A ES530249 A ES 530249A ES 530249 A ES530249 A ES 530249A ES 8603952 A1 ES8603952 A1 ES 8603952A1
Authority
ES
Spain
Prior art keywords
suppression
vibrio cholerae
isolating mutants
cholerae
strain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES530249A
Other languages
English (en)
Other versions
ES530249A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Maryland Baltimore
University of Maryland College Park
Original Assignee
University of Maryland Baltimore
University of Maryland College Park
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Maryland Baltimore, University of Maryland College Park filed Critical University of Maryland Baltimore
Publication of ES530249A0 publication Critical patent/ES530249A0/es
Publication of ES8603952A1 publication Critical patent/ES8603952A1/es
Expired legal-status Critical Current

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

METODO DE AISLAR MUTANTES POR SUPRESION DE VIBRIO CHOLERAE. COMPRENDE: A) CONSTRUIR UN PLASMIDO CON SECUENCIAS FLANQUEANTES DE A DE UN FRAGMENTO DE ENDONUCLEASA DE RESTRICCION SUPRIMIDO Y UN GEN PARA UN MARCADOR SELECCIONABLE LIGADO A LAS SECUENCIAS FLANQUEANTES; B) APAREAR UNA CEPA VIRULENTA DE A, CON UN MICROORGANISMO QUE LLEVA EL PLASMIDO; C) SELECCIONAR A QUE EXPRESA EL PRIMER MARCADOR SELECCIONABLE; D) APAREAR EL PRODUCTO OBTENIDO EN (C) CON UN SGUNDO MICROORGANISMO QUE LLEVA UN SEGUNDO PLASMIDO CON UN SEGUNDO MARCADOR SELECCIONABLE; E) SELECCIONAR A PARA AISLAR UN RECOMBINANTE IN VIVO; F) APAREAR EL RECOMBINANTE IN VIVO CON UN TERCER MICROORGANISMO QUE LLEVA OTRO PLASMIDO, LLEVANDO ESTE UN TERCER MARCADOR SELECCIONABLE; G) SELECCIONAR A QUE EXPRESA EL TERCER MARCADOR SELECCIONABLE; Y H) CURAR DE PLASMIDOS EL PRODUCTO OBTENIDO EN (G). SIENDO A VIBRIO CHOLERAE.
ES530249A 1983-03-04 1984-03-02 Un metodo de aislar mutantes por supresion de vibrio cholerae Expired ES8603952A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US47227683A 1983-03-04 1983-03-04

Publications (2)

Publication Number Publication Date
ES530249A0 ES530249A0 (es) 1986-01-01
ES8603952A1 true ES8603952A1 (es) 1986-01-01

Family

ID=23874842

Family Applications (1)

Application Number Title Priority Date Filing Date
ES530249A Expired ES8603952A1 (es) 1983-03-04 1984-03-02 Un metodo de aislar mutantes por supresion de vibrio cholerae

Country Status (4)

Country Link
JP (1) JPH0630569B2 (es)
ES (1) ES8603952A1 (es)
PT (1) PT78190B (es)
ZA (1) ZA841557B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4882278A (en) * 1983-04-29 1989-11-21 President And Fellows Of Harvard College Non-toxinogenic vibrio cholerae mutants

Also Published As

Publication number Publication date
ZA841557B (en) 1984-10-31
JPH0630569B2 (ja) 1994-04-27
PT78190A (en) 1984-04-01
ES530249A0 (es) 1986-01-01
JPS60110286A (ja) 1985-06-15
PT78190B (en) 1986-04-24

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20050910