ES8605580A1 - Un procedimiento para manipular por ingenieria genetica ce- phalosporium acremonium productor de cefalosporina - Google Patents

Un procedimiento para manipular por ingenieria genetica ce- phalosporium acremonium productor de cefalosporina

Info

Publication number
ES8605580A1
ES8605580A1 ES85548663A ES548663A ES8605580A1 ES 8605580 A1 ES8605580 A1 ES 8605580A1 ES 85548663 A ES85548663 A ES 85548663A ES 548663 A ES548663 A ES 548663A ES 8605580 A1 ES8605580 A1 ES 8605580A1
Authority
ES
Spain
Prior art keywords
cephalosporium acremonium
transformed
resistance marker
aminoglycoside
aminoglycoside resistance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES85548663A
Other languages
English (en)
Other versions
ES548663A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB848428094A external-priority patent/GB8428094D0/en
Priority claimed from GB858503441A external-priority patent/GB8503441D0/en
Priority claimed from GB858508353A external-priority patent/GB8508353D0/en
Application filed by Antibioticos SA filed Critical Antibioticos SA
Publication of ES548663A0 publication Critical patent/ES548663A0/es
Publication of ES8605580A1 publication Critical patent/ES8605580A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/925—Cephalosporium
    • Y10S435/926—Cephalosporium acremonium

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cephalosporin Compounds (AREA)
  • Saccharide Compounds (AREA)

Abstract

METODO DE TRANSFORMACION DE CELULAS DE CEPHALOSPORIUM ACREMONIUM PARA LA OBTENCION DE CEPAS DE CEPHALOSPORIUM ACREMONIUM, QUE POSEEN EN SU GEMONA UN MARCADOR DE RESISTENCIA A AMINOGLICOXIDOS. COMPRENDE LA MEZCLA DE LOS ESFEROPLASTOS CON LA CANTIDAD ADECUADA DE DNA PLASMIDICO EN UN MEDIO DE PH REGULADO, A LA TEMPERATURA AMBIENTE; RECUPERACION DE LOS ESFEROPLASTOS Y CULTIUVARLOS EN UN MEDIO DE REGENERACION DE PROTOPLASTOS A LA TEMPERATURA DE 28JC; Y SELECCIONAR LOS CLONES RESISTENTES A LOS AMINOGLICOXIDOS. ESTE MICROORGANISMO TIENE APLICACIONES PARA LA PRODUCCION DE CEFALOSPORINAS SEMISINTETICAS.
ES85548663A 1984-11-07 1985-11-07 Un procedimiento para manipular por ingenieria genetica ce- phalosporium acremonium productor de cefalosporina Expired ES8605580A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB848428094A GB8428094D0 (en) 1984-11-07 1984-11-07 Cephalosporium acremonium
GB858503441A GB8503441D0 (en) 1985-02-11 1985-02-11 Cephalosporium acremonium
GB858508353A GB8508353D0 (en) 1985-03-29 1985-03-29 Cephalosporium acremonium

Publications (2)

Publication Number Publication Date
ES548663A0 ES548663A0 (es) 1986-03-16
ES8605580A1 true ES8605580A1 (es) 1986-03-16

Family

ID=27262507

Family Applications (1)

Application Number Title Priority Date Filing Date
ES85548663A Expired ES8605580A1 (es) 1984-11-07 1985-11-07 Un procedimiento para manipular por ingenieria genetica ce- phalosporium acremonium productor de cefalosporina

Country Status (4)

Country Link
US (1) US4753877A (es)
EP (1) EP0181213B1 (es)
DE (1) DE3579788D1 (es)
ES (1) ES8605580A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508800D0 (en) * 1985-04-04 1985-05-09 Glaxo Group Ltd Microbiological process
US5198345A (en) * 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi
DE3650783T2 (de) * 1985-04-15 2004-07-15 Dsm Ip Assets B.V. Verwendung des Glucoamylasepromotors aus Apergillus
US20010049612A1 (en) * 2000-05-10 2001-12-06 Corporate Compensation Plans, Inc. Survivor's benefit plan

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3121815A1 (de) * 1981-06-02 1983-02-24 Hoechst Ag, 6000 Frankfurt Hybridvektor, verfahren zu seiner herstellung und seine verwendung
IL66065A (en) * 1981-06-22 1989-06-30 Lilly Co Eli Recombinant dna cloning vectors and the e.coli transformants thereof
DE3141691A1 (de) * 1981-10-21 1983-05-19 Hoechst Ag, 6230 Frankfurt Plasmid pac 1, verfahren zu seiner gewinnung und seine verwendung
CA1207687A (en) * 1982-07-07 1986-07-15 Alan M. Lambowitz Filamentous fungi functional replicating extrachromosomal element
JPS59135895A (ja) * 1982-10-04 1984-08-04 M S C:Kk 抗生物質の生産を促進する機能を有する化合物
US4762786A (en) * 1984-09-27 1988-08-09 Eli Lilly And Company Vectors and conditions which allow genetic transformation of cephalosporium

Also Published As

Publication number Publication date
EP0181213B1 (en) 1990-09-19
ES548663A0 (es) 1986-03-16
EP0181213A2 (en) 1986-05-14
EP0181213A3 (en) 1986-10-29
US4753877A (en) 1988-06-28
DE3579788D1 (de) 1990-10-25

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