ES8609725A1 - Procedimiento para la determinacion de una sustancia capaz de fijarse inmunologicamente - Google Patents

Procedimiento para la determinacion de una sustancia capaz de fijarse inmunologicamente

Info

Publication number
ES8609725A1
ES8609725A1 ES546338A ES546338A ES8609725A1 ES 8609725 A1 ES8609725 A1 ES 8609725A1 ES 546338 A ES546338 A ES 546338A ES 546338 A ES546338 A ES 546338A ES 8609725 A1 ES8609725 A1 ES 8609725A1
Authority
ES
Spain
Prior art keywords
determined
conjugate
enzyme
determination
bindable substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES546338A
Other languages
English (en)
Other versions
ES546338A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of ES8609725A1 publication Critical patent/ES8609725A1/es
Publication of ES546338A0 publication Critical patent/ES546338A0/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO PARA DETERMINAR UNA SUSTANCIA CAPAZ DE FIJARSE INMUNOLOGICAMENTE. COMPRENDE: A) INCUBAR A (A) CON (B, C Y D); B) SEPARAR LAS FASES Y C) DETERMINAR LA ACTIVIDAD ENZIMATICA DE AMBAS FASES, PARA DETERMINAR LA PRESENCIA DE HAPTENOS. SIENDO: (A) UNA SOLUCION DE MUESTRA QUE CONTIENE LA SUSTANCIA A DETERMINAR; (B) UN ANTICUERPO FIJADO A UNA FASE SOLIDA CONTRA LA SUSTANCIA CAPAZ DE FIJARSE Y QUE HA DE SER DETERMINADA Y ELEGIDO ENTRE COMPLEJOS, POLICLONALES O MONOCLONALES; (C) UN CONJUGADO A BASE DE LA SUSTANCIA CAPAZ DE FIJARSE QUE HA DE SER DETERMINADA Y DE UN FRAGMENTO FAB MONOCLONAL DE UN ANTICUERPO DIRIGIDO CONTRA UNA ENZIMA DETERMINABLE EN UNA CANTIDAD DETERMINADA Y OBTENIDO POR CROMATOGRAFIA DE AFINIDAD Y DISOCIACION; (D) UNA ENZIMA QUE CORRESPONDE AL ANTICUERPO DEL CONJUGADO Y SE ELIGE ENTRE PEROXIDASA, FOSFATASA ALCALINA, CATALASA, LISOZIMA Y GLUCOSA-6-FOSFATO-DESHIDROGENASA Y LA FASE SOLIDA O DE SOPORTE SE ELIGE ENTRE VIDRIO, MATERIALES SINTETICOS COMO CELULOSA, DEXTRANO Y POLIMEROS DE ESTIRENO.
ES546338A 1984-08-22 1985-08-22 Procedimiento para la determinacion de una sustancia capaz de fijarse inmunologicamente Expired ES8609725A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843430905 DE3430905A1 (de) 1984-08-22 1984-08-22 Verfahren zur bestimmung einer immunologisch bindefaehigen substanz

Publications (2)

Publication Number Publication Date
ES8609725A1 true ES8609725A1 (es) 1986-08-01
ES546338A0 ES546338A0 (es) 1986-08-01

Family

ID=6243646

Family Applications (1)

Application Number Title Priority Date Filing Date
ES546338A Expired ES8609725A1 (es) 1984-08-22 1985-08-22 Procedimiento para la determinacion de una sustancia capaz de fijarse inmunologicamente

Country Status (7)

Country Link
US (1) US4732848A (es)
EP (1) EP0174528B1 (es)
JP (1) JPS6170462A (es)
AT (1) ATE41242T1 (es)
CA (1) CA1254133A (es)
DE (2) DE3430905A1 (es)
ES (1) ES8609725A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3706718A1 (de) * 1987-03-02 1988-09-15 Boehringer Mannheim Gmbh Vorrichtung zur durchfuehrung einer heterogenen reaktion
US4891313A (en) * 1988-01-21 1990-01-02 Boehringer Manheim Corporation Method for determination of a component of a sample
US4983529A (en) * 1988-06-10 1991-01-08 Abbott Laboratories Immunoassay for HIV-I antigens using F(AB')2 fragments as probe
DE19739218A1 (de) * 1997-09-08 1999-03-11 Boehringer Mannheim Gmbh Aufreinigung von Substanzen aus einer biologischen Probe
WO2002103171A1 (en) * 2001-05-25 2002-12-27 Ibiden Co., Ltd. Alumina-silica-based fiber, ceramic fiber, ceramic fiber complex, retaining seal material, production method thereof, and alumina fiber complex production method

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3940475A (en) * 1970-06-11 1976-02-24 Biological Developments, Inc. Radioimmune method of assaying quantitatively for a hapten
NL171930C (nl) * 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
JPS5344622A (en) * 1976-09-30 1978-04-21 Mochida Pharm Co Ltd Immunologically measuring method
JPS53115314A (en) * 1977-03-17 1978-10-07 Nippon Toki Kk Decorative separating transfer sheet and method of transfer
US4235869A (en) * 1978-05-16 1980-11-25 Syva Company Assay employing a labeled Fab-fragment ligand complex
JPS5537949A (en) * 1978-09-12 1980-03-17 Asahi Chem Ind Co Ltd Measuring method for substance
US4298593A (en) * 1979-08-21 1981-11-03 Abbott Laboratories Reagents and methods utilizing labeled Fab bound to antigens
DE3006709A1 (de) * 1980-02-22 1981-08-27 Hans A. Dipl.-Chem. Dr. 8000 München Thoma Homogenes verfahren zur kompetitiven bestimmung von liganden
US4361647A (en) * 1980-05-22 1982-11-30 Palo Alto Medical Research Foundation Sandwich immunoassay and compositions for use therein
US4423143A (en) * 1980-12-30 1983-12-27 Syva Company β-D-Galactosidase conjugate for enzyme immunoassays
GB2102946B (en) * 1981-06-30 1984-11-28 Wellcome Found Enzyme immunoassay
US4444878A (en) * 1981-12-21 1984-04-24 Boston Biomedical Research Institute, Inc. Bispecific antibody determinants
DE3364347D1 (en) * 1982-04-09 1986-08-07 Fujirebio Kk Anti immune complex antibody and preparation thereof

Also Published As

Publication number Publication date
DE3568639D1 (en) 1989-04-13
ES546338A0 (es) 1986-08-01
DE3430905A1 (de) 1986-02-27
JPS6170462A (ja) 1986-04-11
EP0174528B1 (de) 1989-03-08
ATE41242T1 (de) 1989-03-15
CA1254133A (en) 1989-05-16
EP0174528A1 (de) 1986-03-19
US4732848A (en) 1988-03-22

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