ES8706207A1 - Un procedimiento para la preparacion del plasmido pcz101 - Google Patents

Un procedimiento para la preparacion del plasmido pcz101

Info

Publication number
ES8706207A1
ES8706207A1 ES550012A ES550012A ES8706207A1 ES 8706207 A1 ES8706207 A1 ES 8706207A1 ES 550012 A ES550012 A ES 550012A ES 550012 A ES550012 A ES 550012A ES 8706207 A1 ES8706207 A1 ES 8706207A1
Authority
ES
Spain
Prior art keywords
recombinant dna
translational
expression vectors
codes
reading frame
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES550012A
Other languages
English (en)
Other versions
ES550012A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of ES550012A0 publication Critical patent/ES550012A0/es
Publication of ES8706207A1 publication Critical patent/ES8706207A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

METODO DE PREPARACION DEL PLASMIDO PCZ101. CONSISTE EN: INCUBAR EL DNA OBTENIDO POR DIGESTION DE CADA UNO DE LOS PLASMIDOS PIM-IK-A3 Y PNM789 B CON LAS ENZIMAS DE RESTRICCION XBA I Y BAM HI, EN PRESENCIA DE TRIFOSFATO DE ADENOSINA (ATP) Y DNA LIGASA EN UNA MEZCLA DE LGACION CONVENCIONAL A LA TEMPERATURA AMBIENTE DURANTE DOS HORAS; ELEVAR LA TEMPERATURA DE LA MEZCLA DE INCUBACION DURANTE DOS MINUTOS HASTA UNA TEMPERATURA NECESARIA PARA DESNATURALIZAR CUALQUIER EXTREMO COHESIVO NO LIGADO EN EL DNA INCUBADO; Y RAPIDAMENTE, ENFRIAR LA MEZCLA DE INCUBACION PARA FINALIZAR LA REACCION DE LIGACION. TIENE UTILIDAD PARA PREPARAR COMPOSICIONES FARMACEUTICAS.
ES550012A 1984-03-06 1985-12-16 Un procedimiento para la preparacion del plasmido pcz101 Expired ES8706207A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US58659284A 1984-03-06 1984-03-06

Publications (2)

Publication Number Publication Date
ES550012A0 ES550012A0 (es) 1987-06-01
ES8706207A1 true ES8706207A1 (es) 1987-06-01

Family

ID=24346371

Family Applications (3)

Application Number Title Priority Date Filing Date
ES540936A Expired ES8802322A1 (es) 1984-03-06 1985-03-05 Un procedimiento para la preparacion de un plasmido de expresion de dna recombinante.
ES550013A Expired ES8702495A1 (es) 1984-03-06 1985-12-16 Un metodo de produccion de un polipeptido funcional
ES550012A Expired ES8706207A1 (es) 1984-03-06 1985-12-16 Un procedimiento para la preparacion del plasmido pcz101

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ES540936A Expired ES8802322A1 (es) 1984-03-06 1985-03-05 Un procedimiento para la preparacion de un plasmido de expresion de dna recombinante.
ES550013A Expired ES8702495A1 (es) 1984-03-06 1985-12-16 Un metodo de produccion de un polipeptido funcional

Country Status (17)

Country Link
EP (1) EP0154539A3 (es)
JP (1) JPS611387A (es)
KR (1) KR900001015B1 (es)
CN (1) CN85101555A (es)
AU (1) AU589355B2 (es)
CA (1) CA1283374C (es)
DK (1) DK99985A (es)
DO (1) DOP1985004337A (es)
EG (1) EG17786A (es)
ES (3) ES8802322A1 (es)
GR (1) GR850571B (es)
HU (1) HU202587B (es)
IL (1) IL74507A0 (es)
NZ (1) NZ211313A (es)
PT (1) PT80051B (es)
SU (1) SU1491346A3 (es)
ZA (1) ZA851626B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0159123B1 (en) * 1984-03-06 1992-01-15 Eli Lilly And Company Vectors for expressing bovine growth hormone derivatives
US5618920A (en) 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
AU582288B2 (en) * 1986-03-07 1989-03-16 Damon Biotech Inc. Vector and method for achieving high level expression in eukaryotic cells
FR2599753B1 (fr) * 1986-06-05 1989-12-08 Sanofi Sa Sequence d'adn composite et son application pour la production de l'hormone de croissance
DE3843894A1 (de) * 1988-12-24 1990-06-28 Behringwerke Ag Plasmide mit translations-start-stop-start codon-konfiguration zur expression von proteinen in e.coli

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE55244B1 (en) * 1982-05-25 1990-07-18 Lilly Co Eli Cloning vectors for expression of exogenous protein
US4559300A (en) * 1983-01-18 1985-12-17 Eli Lilly And Company Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
US4713339A (en) * 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
IE56720B1 (en) * 1983-02-07 1991-11-20 Battelle Memorial Institute Methods and compositions for expression of competent eukaryotic gene products
DE3578852D1 (de) * 1984-09-26 1990-08-30 Lilly Co Eli Verfahren zur expression und sekretion in bacillus.
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces

Also Published As

Publication number Publication date
SU1491346A3 (ru) 1989-06-30
ES550012A0 (es) 1987-06-01
NZ211313A (en) 1988-05-30
ES8802322A1 (es) 1988-05-01
DK99985A (da) 1985-09-07
DOP1985004337A (es) 1990-09-17
JPS611387A (ja) 1986-01-07
CN85101555A (zh) 1987-01-24
ES8702495A1 (es) 1987-01-01
EP0154539A2 (en) 1985-09-11
ZA851626B (en) 1986-10-29
PT80051A (en) 1985-04-01
KR850006703A (ko) 1985-10-16
GR850571B (es) 1985-07-08
AU589355B2 (en) 1989-10-12
ES540936A0 (es) 1988-05-01
KR900001015B1 (ko) 1990-02-24
ES550013A0 (es) 1987-01-01
IL74507A0 (en) 1985-06-30
EP0154539A3 (en) 1986-12-30
DK99985D0 (da) 1985-03-05
HUT40162A (en) 1986-11-28
AU3950285A (en) 1985-09-12
HU202587B (en) 1991-03-28
CA1283374C (en) 1991-04-23
EG17786A (en) 1990-10-30
PT80051B (en) 1987-03-24

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Legal Events

Date Code Title Description
MM4A Patent lapsed

Effective date: 19960401