FI112949B - Proteiinien erottaminen - Google Patents

Proteiinien erottaminen Download PDF

Info

Publication number: FI112949B
Authority: FI; Finland
Prior art keywords: protein; peg; crystallization; enzyme; added
Prior art date: 1993-07-09

Application number

FI960073A

Other languages

English (en)

Finnish (fi)

Swedish (sv)

Other versions

FI960073L (fi

FI960073A0 (fi

Inventor

Birgitte Mahler Nilsson

Mads Aage Laustsen

Anders Rancke-Madsen

Original Assignee

Novozymes As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1993-07-09

Filing date

1996-01-08

Publication date

2004-02-13

1996-01-08 Application filed by Novozymes As filed Critical Novozymes As

1996-01-08 Publication of FI960073L publication Critical patent/FI960073L/fi

1996-01-08 Publication of FI960073A0 publication Critical patent/FI960073A0/fi

2004-02-13 Application granted granted Critical

2004-02-13 Publication of FI112949B publication Critical patent/FI112949B/fi

Links

108090000623 proteins and genes Proteins 0.000 title claims abstract description 49
102000004169 proteins and genes Human genes 0.000 title claims abstract description 47
238000000926 separation method Methods 0.000 title description 12
238000000034 method Methods 0.000 claims abstract description 46
150000003839 salts Chemical class 0.000 claims abstract description 18
239000000203 mixture Substances 0.000 claims abstract description 12
238000002425 crystallisation Methods 0.000 claims description 45
230000008025 crystallization Effects 0.000 claims description 44
229920001223 polyethylene glycol Polymers 0.000 claims description 35
108091005804 Peptidases Proteins 0.000 claims description 16
239000004365 Protease Substances 0.000 claims description 14
239000002202 Polyethylene glycol Substances 0.000 claims description 13
102000004316 Oxidoreductases Human genes 0.000 claims description 9
108090000854 Oxidoreductases Proteins 0.000 claims description 9
108010084185 Cellulases Proteins 0.000 claims description 6
102000005575 Cellulases Human genes 0.000 claims description 6
102000035195 Peptidases Human genes 0.000 claims description 5
239000001963 growth medium Substances 0.000 claims description 4
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 2
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 2
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 claims description 2
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 2
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 2
150000001450 anions Chemical class 0.000 claims description 2
239000011575 calcium Substances 0.000 claims description 2
229910052791 calcium Inorganic materials 0.000 claims description 2
239000011777 magnesium Substances 0.000 claims description 2
229910052749 magnesium Inorganic materials 0.000 claims description 2
229920001451 polypropylene glycol Polymers 0.000 claims description 2
239000011591 potassium Substances 0.000 claims description 2
229910052700 potassium Inorganic materials 0.000 claims description 2
239000011734 sodium Substances 0.000 claims description 2
229910052708 sodium Inorganic materials 0.000 claims description 2
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 10
WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 claims 5
150000001805 chlorine compounds Chemical group 0.000 claims 1
102000004190 Enzymes Human genes 0.000 abstract description 50
108090000790 Enzymes Proteins 0.000 abstract description 50
229920003169 water-soluble polymer Polymers 0.000 abstract description 9
238000011084 recovery Methods 0.000 abstract description 5
239000007864 aqueous solution Substances 0.000 abstract description 3
229940088598 enzyme Drugs 0.000 description 48
229920001030 Polyethylene Glycol 4000 Polymers 0.000 description 32
102000003992 Peroxidases Human genes 0.000 description 31
108040007629 peroxidase activity proteins Proteins 0.000 description 29
239000000243 solution Substances 0.000 description 25
102000016938 Catalase Human genes 0.000 description 18
108010053835 Catalase Proteins 0.000 description 18
239000013078 crystal Substances 0.000 description 16
239000012071 phase Substances 0.000 description 16
239000007788 liquid Substances 0.000 description 13
239000007787 solid Substances 0.000 description 13
239000012141 concentrate Substances 0.000 description 12
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 11
102000004882 Lipase Human genes 0.000 description 10
108090001060 Lipase Proteins 0.000 description 10
BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 10
239000004367 Lipase Substances 0.000 description 9
235000019421 lipase Nutrition 0.000 description 9
244000251987 Coprinus macrorhizus Species 0.000 description 7
238000005119 centrifugation Methods 0.000 description 7
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
241000193830 Bacillus <bacterium> Species 0.000 description 6
108010059892 Cellulase Proteins 0.000 description 6
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
230000000694 effects Effects 0.000 description 6
238000001914 filtration Methods 0.000 description 6
239000000126 substance Substances 0.000 description 6
OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 5
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 5
241000223255 Scytalidium Species 0.000 description 5
239000001110 calcium chloride Substances 0.000 description 5
235000011148 calcium chloride Nutrition 0.000 description 5
229910001628 calcium chloride Inorganic materials 0.000 description 5
229940106157 cellulase Drugs 0.000 description 5
238000005189 flocculation Methods 0.000 description 5
230000016615 flocculation Effects 0.000 description 5
235000019253 formic acid Nutrition 0.000 description 5
244000005700 microbiome Species 0.000 description 5
102000013142 Amylases Human genes 0.000 description 4
108010065511 Amylases Proteins 0.000 description 4
235000001673 Coprinus macrorhizus Nutrition 0.000 description 4
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
241000589516 Pseudomonas Species 0.000 description 4
235000019418 amylase Nutrition 0.000 description 4
238000001704 evaporation Methods 0.000 description 4
230000008020 evaporation Effects 0.000 description 4
239000000706 filtrate Substances 0.000 description 4
230000002538 fungal effect Effects 0.000 description 4
238000000108 ultra-filtration Methods 0.000 description 4
241000222511 Coprinus Species 0.000 description 3
108010029541 Laccase Proteins 0.000 description 3
108091028043 Nucleic acid sequence Proteins 0.000 description 3
229920002535 Polyethylene Glycol 1500 Polymers 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical group [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
241000205174 Thermofilum Species 0.000 description 3
102000004142 Trypsin Human genes 0.000 description 3
108090000631 Trypsin Proteins 0.000 description 3
239000002253 acid Substances 0.000 description 3
229940025131 amylases Drugs 0.000 description 3
230000001580 bacterial effect Effects 0.000 description 3
230000015572 biosynthetic process Effects 0.000 description 3
-1 carbohydrate compounds Chemical class 0.000 description 3
239000003960 organic solvent Substances 0.000 description 3
239000012460 protein solution Substances 0.000 description 3
VUYXVWGKCKTUMF-UHFFFAOYSA-N tetratriacontaethylene glycol monomethyl ether Chemical compound COCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO VUYXVWGKCKTUMF-UHFFFAOYSA-N 0.000 description 3
239000012588 trypsin Substances 0.000 description 3
238000005199 ultracentrifugation Methods 0.000 description 3
UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 2
241000228212 Aspergillus Species 0.000 description 2
241000283690 Bos taurus Species 0.000 description 2
101100083507 Caenorhabditis elegans acl-2 gene Proteins 0.000 description 2
241000222120 Candida <Saccharomycetales> Species 0.000 description 2
241000196324 Embryophyta Species 0.000 description 2
241000223218 Fusarium Species 0.000 description 2
108010001336 Horseradish Peroxidase Proteins 0.000 description 2
241000223198 Humicola Species 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
241001465754 Metazoa Species 0.000 description 2
108700020962 Peroxidase Proteins 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
239000008346 aqueous phase Substances 0.000 description 2
238000012258 culturing Methods 0.000 description 2
238000011026 diafiltration Methods 0.000 description 2
239000012535 impurity Substances 0.000 description 2
239000002609 medium Substances 0.000 description 2
230000000813 microbial effect Effects 0.000 description 2
239000011859 microparticle Substances 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
AWFYPPSBLUWMFQ-UHFFFAOYSA-N 2-[5-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-1,3,4-oxadiazol-2-yl]-1-(1,4,6,7-tetrahydropyrazolo[4,3-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C1=NN=C(O1)CC(=O)N1CC2=C(CC1)NN=C2 AWFYPPSBLUWMFQ-UHFFFAOYSA-N 0.000 description 1
ZTOJFFHGPLIVKC-UHFFFAOYSA-N 3-ethyl-2-[(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound S1C2=CC(S(O)(=O)=O)=CC=C2N(CC)C1=NN=C1SC2=CC(S(O)(=O)=O)=CC=C2N1CC ZTOJFFHGPLIVKC-UHFFFAOYSA-N 0.000 description 1
239000004382 Amylase Substances 0.000 description 1
240000006439 Aspergillus oryzae Species 0.000 description 1
235000002247 Aspergillus oryzae Nutrition 0.000 description 1
241000193422 Bacillus lentus Species 0.000 description 1
241000194103 Bacillus pumilus Species 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
108091005658 Basic proteases Proteins 0.000 description 1
241001157813 Cercospora Species 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
241000223208 Curvularia Species 0.000 description 1
108020005199 Dehydrogenases Proteins 0.000 description 1
241000233866 Fungi Species 0.000 description 1
241001480714 Humicola insolens Species 0.000 description 1
241001237944 Hygrophorus Species 0.000 description 1
241000235395 Mucor Species 0.000 description 1
241000226677 Myceliophthora Species 0.000 description 1
GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
108090000417 Oxygenases Proteins 0.000 description 1
102000004020 Oxygenases Human genes 0.000 description 1
241000228143 Penicillium Species 0.000 description 1
241000222385 Phanerochaete Species 0.000 description 1
239000004698 Polyethylene Substances 0.000 description 1
229920002556 Polyethylene Glycol 300 Polymers 0.000 description 1
241000222640 Polyporus Species 0.000 description 1
101710180012 Protease 7 Proteins 0.000 description 1
244000088415 Raphanus sativus Species 0.000 description 1
235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
241001361634 Rhizoctonia Species 0.000 description 1
102000012479 Serine Proteases Human genes 0.000 description 1
108010022999 Serine Proteases Proteins 0.000 description 1
241000187747 Streptomyces Species 0.000 description 1
102000005158 Subtilisins Human genes 0.000 description 1
108010056079 Subtilisins Proteins 0.000 description 1
241000223258 Thermomyces lanuginosus Species 0.000 description 1
241000222354 Trametes Species 0.000 description 1
241000223259 Trichoderma Species 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
102000004139 alpha-Amylases Human genes 0.000 description 1
108090000637 alpha-Amylases Proteins 0.000 description 1
229910000147 aluminium phosphate Inorganic materials 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
229920001222 biopolymer Polymers 0.000 description 1
230000003197 catalytic effect Effects 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
239000003638 chemical reducing agent Substances 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
238000010367 cloning Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
239000008367 deionised water Substances 0.000 description 1
229910021641 deionized water Inorganic materials 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
238000002050 diffraction method Methods 0.000 description 1
238000001035 drying Methods 0.000 description 1
210000002257 embryonic structure Anatomy 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
150000002334 glycols Chemical class 0.000 description 1
210000004185 liver Anatomy 0.000 description 1
238000000464 low-speed centrifugation Methods 0.000 description 1
210000001161 mammalian embryo Anatomy 0.000 description 1
239000000463 material Substances 0.000 description 1
238000001471 micro-filtration Methods 0.000 description 1
239000011325 microbead Substances 0.000 description 1
238000002156 mixing Methods 0.000 description 1
229910017604 nitric acid Inorganic materials 0.000 description 1
230000033116 oxidation-reduction process Effects 0.000 description 1
238000010979 pH adjustment Methods 0.000 description 1
230000006320 pegylation Effects 0.000 description 1
125000000864 peroxy group Chemical group O(O*)* 0.000 description 1
238000005191 phase separation Methods 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
229920000768 polyamine Polymers 0.000 description 1
229920000573 polyethylene Polymers 0.000 description 1
229920001184 polypeptide Polymers 0.000 description 1
230000003389 potentiating effect Effects 0.000 description 1
239000002243 precursor Substances 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
239000000047 product Substances 0.000 description 1
230000006920 protein precipitation Effects 0.000 description 1
238000001742 protein purification Methods 0.000 description 1
238000001953 recrystallisation Methods 0.000 description 1
238000010850 salt effect Methods 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
238000003756 stirring Methods 0.000 description 1
239000011550 stock solution Substances 0.000 description 1
238000013060 ultrafiltration and diafiltration Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Crystallography & Structural Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Analytical Chemistry (AREA)
Enzymes And Modification Thereof (AREA)
Peptides Or Proteins (AREA)
Separation Using Semi-Permeable Membranes (AREA)
Medicines Containing Plant Substances (AREA)
Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

FI960073A 1993-07-09 1996-01-08 Proteiinien erottaminen FI112949B (fi)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
DK83093		1993-07-09
DK93830A DK83093D0 (da)	1993-07-09	1993-07-09	Fremgangsmaade
DK9400256		1994-06-23
PCT/DK1994/000256 WO1995001989A1 (en)	1993-07-09	1994-06-23	Separation of proteins

Publications (3)

Publication Number	Publication Date
FI960073L FI960073L (fi)	1996-01-08
FI960073A0 FI960073A0 (fi)	1996-01-08
FI112949B true FI112949B (fi)	2004-02-13

Family

ID=8098038

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
FI960073A FI112949B (fi)	1993-07-09	1996-01-08	Proteiinien erottaminen

Country Status (13)

Country	Link
US (1)	US5728559A (de)
EP (1)	EP0707594B1 (de)
JP (1)	JPH08512294A (de)
CN (1)	CN1054159C (de)
AT (1)	ATE209214T1 (de)
AU (1)	AU7121494A (de)
BR (1)	BR9407047A (de)
DE (1)	DE69429172T2 (de)
DK (2)	DK83093D0 (de)
ES (1)	ES2168300T3 (de)
FI (1)	FI112949B (de)
PT (1)	PT707594E (de)
WO (1)	WO1995001989A1 (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE69734708T2 (de) *	1996-03-08	2006-07-27	Novozymes A/S	Kristallisation eines proteins mit einem schwefelsalz
US6207437B1 (en)	1996-03-11	2001-03-27	Genencor International, Inc.	Crystalline protease and method for producing same
JP2000506176A (ja) *	1996-03-14	2000-05-23	ノボノルディスクアクティーゼルスカブ	固体吸着物質の使用による結晶化タンパク質の高収量化
US6031082A (en) *	1996-03-14	2000-02-29	Novo Nordisk A/S	Increased yields of a crystallized protein by using a solid adsorption material
WO1997034919A1 (en) *	1996-03-15	1997-09-25	Novo Nordisk A/S	Method for purification of a protein from a protein containing solution
NZ514732A (en) *	1999-04-06	2004-01-30	Uab Research Foundation	Method for screening crystallization conditions in solution crystal growth
US7247490B2 (en) *	1999-04-06	2007-07-24	Uab Research Foundation	Method for screening crystallization conditions in solution crystal growth
US20030022383A1 (en) *	1999-04-06	2003-01-30	Uab Research Foundation	Method for screening crystallization conditions in solution crystal growth
US7244396B2 (en) *	1999-04-06	2007-07-17	Uab Research Foundation	Method for preparation of microarrays for screening of crystal growth conditions
US7214540B2 (en) *	1999-04-06	2007-05-08	Uab Research Foundation	Method for screening crystallization conditions in solution crystal growth
US7250305B2 (en) *	2001-07-30	2007-07-31	Uab Research Foundation	Use of dye to distinguish salt and protein crystals under microcrystallization conditions
EP1289629B1 (de) *	2000-05-18	2004-08-04	Novozymes A/S	Mikrofiltration unter verwendung von aktivkohle
US6663602B2 (en) *	2000-06-16	2003-12-16	Novo Nordisk A/S	Injection device
US7670429B2 (en) *	2001-04-05	2010-03-02	The California Institute Of Technology	High throughput screening of crystallization of materials
US7118891B2 (en)	2001-12-11	2006-10-10	Novozymes A/S	Crystal harvest from fermentation broth
CN100487114C (zh) *	2001-12-11	2009-05-13	诺维信公司	从发酵液收获晶体
US20070026528A1 (en) *	2002-05-30	2007-02-01	Delucas Lawrence J	Method for screening crystallization conditions in solution crystal growth
WO2004005898A1 (en) *	2002-07-10	2004-01-15	Uab Research Foundation	Method for distinguishing between biomolecule and non-biomolecule crystals
US20040185449A1 (en) *	2003-03-20	2004-09-23	Quinn John J.	Method for preparing assay samples
AU2004269196B2 (en) *	2003-09-03	2010-03-04	Shmuel Bukshpan	Methods and apparatus for rapid crystallization of biomolecules
US7507552B1 (en)	2004-04-16	2009-03-24	Takeda San Diego, Inc.	Crystallization of histone deacetylase 2
US7686786B2 (en)	2004-10-21	2010-03-30	Novo Nordiks A/S	Dial-down mechanism for wind-up pen
CN101163514B (zh) *	2005-04-24	2012-01-25	诺和诺德公司	注射装置
WO2007104697A2 (en) *	2006-03-10	2007-09-20	Novo Nordisk A/S	An injection device having a gearing arrangement
WO2007104636A1 (en) *	2006-03-10	2007-09-20	Novo Nordisk A/S	An injection device and a method of changing a cartridge in the device
EP2004671B1 (de) *	2006-03-30	2015-12-09	Novo Nordisk A/S	Zwei-phasen fällung von proteinen
ATE458517T1 (de)	2006-05-16	2010-03-15	Novo Nordisk As	Getriebemechanismus für ein injektionsgerät
PL2023982T3 (pl) *	2006-05-18	2012-11-30	Novo Nordisk As	Urządzenie wstrzykujące ze środkami blokującymi
FR2902799B1 (fr)	2006-06-27	2012-10-26	Millipore Corp	Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8569464B2 (en)	2006-12-21	2013-10-29	Emd Millipore Corporation	Purification of proteins
US8362217B2 (en)	2006-12-21	2013-01-29	Emd Millipore Corporation	Purification of proteins
WO2008079280A1 (en)	2006-12-21	2008-07-03	Millipore Corporation	Purification of proteins
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1993
- 1993-07-09 DK DK93830A patent/DK83093D0/da not_active Application Discontinuation
1994
- 1994-06-23 CN CN94192657A patent/CN1054159C/zh not_active Expired - Fee Related
- 1994-06-23 ES ES94920412T patent/ES2168300T3/es not_active Expired - Lifetime
- 1994-06-23 EP EP94920412A patent/EP0707594B1/de not_active Expired - Lifetime
- 1994-06-23 BR BR9407047A patent/BR9407047A/pt not_active IP Right Cessation
- 1994-06-23 JP JP7503762A patent/JPH08512294A/ja not_active Ceased
- 1994-06-23 US US08/557,056 patent/US5728559A/en not_active Expired - Lifetime
- 1994-06-23 WO PCT/DK1994/000256 patent/WO1995001989A1/en not_active Ceased
- 1994-06-23 DK DK94920412T patent/DK0707594T3/da active
- 1994-06-23 AT AT94920412T patent/ATE209214T1/de not_active IP Right Cessation
- 1994-06-23 DE DE69429172T patent/DE69429172T2/de not_active Expired - Fee Related
- 1994-06-23 PT PT94920412T patent/PT707594E/pt unknown
- 1994-06-23 AU AU71214/94A patent/AU7121494A/en not_active Abandoned
1996
- 1996-01-08 FI FI960073A patent/FI112949B/fi active

Also Published As

Publication number	Publication date
DE69429172D1 (de)	2002-01-03
FI960073L (fi)	1996-01-08
EP0707594B1 (de)	2001-11-21
US5728559A (en)	1998-03-17
FI960073A0 (fi)	1996-01-08
AU7121494A (en)	1995-02-06
ATE209214T1 (de)	2001-12-15
EP0707594A1 (de)	1996-04-24
DK0707594T3 (da)	2002-05-21
ES2168300T3 (es)	2002-06-16
DK83093D0 (da)	1993-07-09
CN1126477A (zh)	1996-07-10
WO1995001989A1 (en)	1995-01-19
JPH08512294A (ja)	1996-12-24
PT707594E (pt)	2002-05-31
DE69429172T2 (de)	2002-07-11
BR9407047A (pt)	1996-08-13
CN1054159C (zh)	2000-07-05

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