FI117202B - Yhdistelmä-DNA-tekniikalla saadut solut, DNA-kontruktit, vektorit ja menetelmät fytaattia hajottavien entsyymien ilmentämiseksi toivotuissa suhteissa - Google Patents

Yhdistelmä-DNA-tekniikalla saadut solut, DNA-kontruktit, vektorit ja menetelmät fytaattia hajottavien entsyymien ilmentämiseksi toivotuissa suhteissa Download PDF

Info

Publication number: FI117202B
Authority: FI; Finland
Prior art keywords: sequence; ser; ala; leu; gly
Prior art date: 1992-07-31

Application number

FI950409A

Other languages

English (en)

Finnish (fi)

Swedish (sv)

Other versions

FI950409A0 (fi

FI950409L (fi

Inventor

John A Rambosek

Helena K M Nevalainen

Marja T Paloheimo

Richard B Fagerstroem

Arja S K Miettinen-Oinonen

Marja K Turunen

Christopher S Piddington

Christine S Houston

Michael A Cantrell

Original Assignee

Ab Enzymes Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-07-31

Filing date

1995-01-30

Publication date

2006-07-31

1995-01-30 Application filed by Ab Enzymes Gmbh filed Critical Ab Enzymes Gmbh

1995-01-30 Publication of FI950409A0 publication Critical patent/FI950409A0/fi

1995-03-30 Publication of FI950409L publication Critical patent/FI950409L/fi

2006-07-31 Application granted granted Critical

2006-07-31 Publication of FI117202B publication Critical patent/FI117202B/fi

Links

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BUDNAJYVCUHLSV-ZLUOBGJFSA-N Ala-Asp-Ser Chemical compound C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(O)=O BUDNAJYVCUHLSV-ZLUOBGJFSA-N 0.000 claims 1
JQDFGZKKXBEANU-IMJSIDKUSA-N Ala-Cys Chemical compound C[C@H](N)C(=O)N[C@@H](CS)C(O)=O JQDFGZKKXBEANU-IMJSIDKUSA-N 0.000 claims 1
HHRAXZAYZFFRAM-CIUDSAMLSA-N Ala-Leu-Asn Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O HHRAXZAYZFFRAM-CIUDSAMLSA-N 0.000 claims 1
MNZHHDPWDWQJCQ-YUMQZZPRSA-N Ala-Leu-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O MNZHHDPWDWQJCQ-YUMQZZPRSA-N 0.000 claims 1
DHBKYZYFEXXUAK-ONGXEEELSA-N Ala-Phe-Gly Chemical compound OC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](N)C)CC1=CC=CC=C1 DHBKYZYFEXXUAK-ONGXEEELSA-N 0.000 claims 1
DCVYRWFAMZFSDA-ZLUOBGJFSA-N Ala-Ser-Ala Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O DCVYRWFAMZFSDA-ZLUOBGJFSA-N 0.000 claims 1
RMAWDDRDTRSZIR-ZLUOBGJFSA-N Ala-Ser-Asp Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O RMAWDDRDTRSZIR-ZLUOBGJFSA-N 0.000 claims 1
PEEYDECOOVQKRZ-DLOVCJGASA-N Ala-Ser-Phe Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O PEEYDECOOVQKRZ-DLOVCJGASA-N 0.000 claims 1
NCQMBSJGJMYKCK-ZLUOBGJFSA-N Ala-Ser-Ser Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O NCQMBSJGJMYKCK-ZLUOBGJFSA-N 0.000 claims 1
SYIFFFHSXBNPMC-UWJYBYFXSA-N Ala-Ser-Tyr Chemical compound C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)O)N SYIFFFHSXBNPMC-UWJYBYFXSA-N 0.000 claims 1
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OEVCHROQUIVQFZ-YTLHQDLWSA-N Ala-Thr-Ala Chemical compound C[C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@@H](C)C(O)=O OEVCHROQUIVQFZ-YTLHQDLWSA-N 0.000 claims 1
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AOAKQKVICDWCLB-UWJYBYFXSA-N Ala-Tyr-Asn Chemical compound C[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)N AOAKQKVICDWCLB-UWJYBYFXSA-N 0.000 claims 1
VHAQSYHSDKERBS-XPUUQOCRSA-N Ala-Val-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)NCC(O)=O VHAQSYHSDKERBS-XPUUQOCRSA-N 0.000 claims 1
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ITYRYNUZHPNCIK-GUBZILKMSA-N Glu-Ala-Leu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(O)=O ITYRYNUZHPNCIK-GUBZILKMSA-N 0.000 claims 1
NCWOMXABNYEPLY-NRPADANISA-N Glu-Ala-Val Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(O)=O NCWOMXABNYEPLY-NRPADANISA-N 0.000 claims 1
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CAQXJMUDOLSBPF-SUSMZKCASA-N Glu-Thr-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O CAQXJMUDOLSBPF-SUSMZKCASA-N 0.000 claims 1
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MFYLRRCYBBJYPI-JYJNAYRXSA-N Glu-Tyr-Lys Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)O)N)O MFYLRRCYBBJYPI-JYJNAYRXSA-N 0.000 claims 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Polymers & Plastics (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Food Science & Technology (AREA)
Animal Husbandry (AREA)
Mycology (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Processing Of Solid Wastes (AREA)
Saccharide Compounds (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

FI950409A 1992-07-31 1995-01-30 Yhdistelmä-DNA-tekniikalla saadut solut, DNA-kontruktit, vektorit ja menetelmät fytaattia hajottavien entsyymien ilmentämiseksi toivotuissa suhteissa FI117202B (fi)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
US92540192A	1992-07-31	1992-07-31
US92540192		1992-07-31
US9307058		1993-07-27
PCT/US1993/007058 WO1994003072A1 (en)	1992-07-31	1993-07-27	Recombinant cells, dna constructs, vectors and methods for expressing phytate degrading enzymes in desired ratios

Publications (3)

Publication Number	Publication Date
FI950409A0 FI950409A0 (fi)	1995-01-30
FI950409L FI950409L (fi)	1995-03-30
FI117202B true FI117202B (fi)	2006-07-31

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FI950409A FI117202B (fi)	1992-07-31	1995-01-30	Yhdistelmä-DNA-tekniikalla saadut solut, DNA-kontruktit, vektorit ja menetelmät fytaattia hajottavien entsyymien ilmentämiseksi toivotuissa suhteissa

Country Status (12)

Country	Link
US (1)	US5834286A (de)
EP (1)	EP0655890B1 (de)
JP (1)	JPH09505201A (de)
AT (1)	ATE287962T1 (de)
AU (1)	AU4787993A (de)
CA (1)	CA2141431A1 (de)
DE (1)	DE69333747T2 (de)
DK (1)	DK0655890T3 (de)
ES (1)	ES2235157T3 (de)
FI (1)	FI117202B (de)
PT (1)	PT655890E (de)
WO (1)	WO1994003072A1 (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5780292A (en) *	1987-04-29	1998-07-14	Alko Group Ltd.	Production of phytate degrading enzymes in trichoderma
JPH09505201A (ja) *	1992-07-31	1997-05-27	パンラブスインコーポレイテッド	組換え細胞、ｄｎａ構造、ベクター及びフィチン酸塩分解酵素を任意の比率で発現する方法
US6358722B1 (en)	1994-04-25	2002-03-19	Roche Vitamins, Inc.	Heat tolerant phytases
US6291221B1 (en)	1994-04-25	2001-09-18	Roche Vitamins Inc.	Heat tolerant phytases
US6699704B1 (en)	1994-04-25	2004-03-02	Roche Vitamins Inc.	Heat tolerant phytases
US5830732A (en) *	1994-07-05	1998-11-03	Mitsui Toatsu Chemicals, Inc.	Phytase
US6635464B1 (en)	1995-12-18	2003-10-21	Rohm Enzyme Finland Oy	Xylanases, genes encoding them, and uses thereof
US5985605A (en) *	1996-06-14	1999-11-16	Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada	DNA sequences encoding phytases of ruminal microorganisms
US5939303A (en) *	1996-06-14	1999-08-17	Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada	Phytases of ruminal microorganisms
KR20000036143A (ko)	1996-09-25	2000-06-26	히라다 다다시	신규피틴산 분해효소 및 그의 제조방법
JP2001512306A (ja) *	1997-01-08	2001-08-21	ライフテクノロジーズ，インコーポレイテッド	タンパク質の産生方法
NZ501409A (en) *	1997-06-04	2002-02-01	Dsm N	High-activity phytase compositions containing a carbohydrate polymer
NZ330940A (en)	1997-07-24	2000-02-28	F	Production of consensus phytases from fungal origin using computer programmes
US5977435A (en) *	1997-10-21	1999-11-02	Performance Plants, Inc.	Plant phosphatases
US6451572B1 (en)	1998-06-25	2002-09-17	Cornell Research Foundation, Inc.	Overexpression of phytase genes in yeast systems
MXPA01009809A (es) *	1999-03-31	2002-07-30	Cornell Res Foundation Inc	Fosfatasas con actividad de fitasa mejorada.
US6303766B1 (en)	1999-05-14	2001-10-16	Virginia Tech Intellectual Properties, Inc.	Soybean phytase and nucleic acid encoding the same
US6841370B1 (en)	1999-11-18	2005-01-11	Cornell Research Foundation, Inc.	Site-directed mutagenesis of Escherichia coli phytase
AU2001267869A1 (en) *	2000-06-28	2002-01-08	Kyowa Hakko Kogyo Co. Ltd.	Transformation system of fungus belonging to the genus monascus
ES2597503T3 (es)	2001-10-31	2017-01-19	Huvepharma Eood	Alimento para animales que contiene fitasa y método
JP3779600B2 (ja) *	2001-11-27	2006-05-31	株式会社日立製作所	Ｐｃｒ用プライマーおよびｐｃｒ用プライマーの塩基配列決定法
SE0200911D0 (sv) *	2002-03-22	2002-03-22	Chalmers Technology Licensing	Phytase active yeast
KR100472082B1 (ko) *	2002-04-13	2005-02-21	주식회사 에이스바이오텍	재조합 단백질의 생산성 증가를 위한 재조합 효모의 배양방법
US7309505B2 (en)	2002-09-13	2007-12-18	Cornell Research Foundation, Inc.	Using mutations to improve Aspergillus phytases
CA2413240A1 (en) *	2002-11-29	2004-05-29	Mcn Bioproducts Inc.	Purification of inositol from plant materials
US7781173B2 (en)	2003-09-25	2010-08-24	Life Technologies Corporation	Homogeneous populations of molecules
WO2006063588A1 (en) *	2004-12-13	2006-06-22	Novozymes A/S	Polypeptides having acid phosphatase activity and polynucleotides encoding same
US7919297B2 (en) *	2006-02-21	2011-04-05	Cornell Research Foundation, Inc.	Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
US20090087873A1 (en)	2006-07-21	2009-04-02	Invitrogen Corporation	Sharply resolving labeled protein molecular weight standards
WO2008017066A2 (en)	2006-08-03	2008-02-07	Cornell Research Foundation, Inc.	Phytases with improved thermal stability
US8192734B2 (en)	2007-07-09	2012-06-05	Cornell University	Compositions and methods for bone strengthening
US9493790B2 (en)	2011-08-24	2016-11-15	Novozymes, Inc.	Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
EP2748321A2 (de) *	2011-08-24	2014-07-02	Novozymes, Inc.	Verfahren zur gewinnung von positiven transformanten einer fadenpilzwirtszelle
KR20190048732A (ko)	2017-10-31	2019-05-09	일동제약(주)	염증의 예방 및 치료 효과를 갖는 락토바실러스 존소니 아이디씨씨 9203 및 이의 용도
KR20190048739A (ko)	2017-10-31	2019-05-09	일동제약(주)	염증의 예방 또는 개선 효능을 갖는 락토바실러스 존소니 아디씨씨 9203 및 이의 용도
EP3670653A1 (de)	2018-12-21	2020-06-24	AB Enzymes Oy	Polypeptide mit phytaseaktivität

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3297548A (en) *	1964-07-28	1967-01-10	Int Minerals & Chem Corp	Preparation of acid phytase
US3966971A (en) *	1975-01-02	1976-06-29	Grain Processing Corporation	Separation of protein from vegetable sources
DE3650202T3 (de) *	1985-08-29	2004-06-03	Genencor International, Inc., South San Francisco	Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung.
US5298405A (en) *	1986-04-30	1994-03-29	Alko Limited	Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production
GB8610600D0 (en) *	1986-04-30	1986-06-04	Novo Industri As	Transformation of trichoderma
FI881496A7 (fi) *	1987-04-06	1988-10-07	Gist Brocades Nv	Menetelmä aminohappojen valmistamiseksi tai uuttamiseksi lannasta
NL8702735A (nl) *	1987-11-17	1989-06-16	Dorr Oliver Inc	Werkwijze voor het weken van granen met een nieuw enzympreparaat.
CA2044240A1 (en) *	1989-01-25	1990-07-26	Maarit Simell	Method for production of phytate-free or low-phytate soy protein isolate and concentrate
UA27702C2 (uk) *	1989-09-27	2000-10-16	Гіст-Брокейдс Н.В.	Фрагмент геномної днк, що кодує фітазу aspergillus niger,фрагмент кднк, що кодує фітазу aspergillus niger, рекомбінантна плазмідна днк для експресії фітази в aspergillus (варіанти), штам aspergillus-продуцент фітази aspergillus (варіанти), спосіб одержання фітази, рекомбінанта фітаза aspergillus niger
GB8921745D0 (en) *	1989-09-27	1989-11-08	Aerosol Inventions Dev	Pressurised dispensers
CZ289014B6 (cs) *	1989-09-27	2001-10-17	Dsm N. V.	Vyčiątěná a izolovaná sekvence DNA kódující fungální fytázu, konstrukt pro expresi, vektory a transformované hostitelské buňky a způsob produkce fytázy
US5593963A (en) *	1990-09-21	1997-01-14	Mogen International	Expression of phytase in plants
KR100225087B1 (ko) *	1990-03-23	1999-10-15	한스 발터라벤	피타아제의 식물내 발현
CA2084189A1 (en) *	1990-07-16	1992-01-17	Alko Group Limited	Immunoglobulin production by trichoderma
US5217959A (en) *	1990-09-06	1993-06-08	Robert Sabin	Method of treating multiple sclerosis with phytic acid
JPH09505201A (ja) *	1992-07-31	1997-05-27	パンラブスインコーポレイテッド	組換え細胞、ｄｎａ構造、ベクター及びフィチン酸塩分解酵素を任意の比率で発現する方法
DE69333071T2 (de) *	1993-04-05	2004-05-06	Aveve N.V.	Phytate-Hydrolyse und enzymatische Zusammensetzung für die Hydrolyse von Phytat

1993
- 1993-07-27 JP JP6505394A patent/JPH09505201A/ja active Pending
- 1993-07-27 PT PT93918426T patent/PT655890E/pt unknown
- 1993-07-27 CA CA002141431A patent/CA2141431A1/en not_active Abandoned
- 1993-07-27 DK DK93918426T patent/DK0655890T3/da active
- 1993-07-27 AU AU47879/93A patent/AU4787993A/en not_active Abandoned
- 1993-07-27 EP EP93918426A patent/EP0655890B1/de not_active Expired - Lifetime
- 1993-07-27 DE DE69333747T patent/DE69333747T2/de not_active Expired - Lifetime
- 1993-07-27 ES ES93918426T patent/ES2235157T3/es not_active Expired - Lifetime
- 1993-07-27 WO PCT/US1993/007058 patent/WO1994003072A1/en not_active Ceased
- 1993-07-27 AT AT93918426T patent/ATE287962T1/de not_active IP Right Cessation
- 1993-07-27 US US08/374,652 patent/US5834286A/en not_active Expired - Lifetime
1995
- 1995-01-30 FI FI950409A patent/FI117202B/fi active IP Right Grant

Also Published As

Publication number	Publication date
US5834286A (en)	1998-11-10
AU4787993A (en)	1994-03-03
PT655890E (pt)	2005-05-31
ATE287962T1 (de)	2005-02-15
EP0655890A4 (de)	1997-04-09
EP0655890A1 (de)	1995-06-07
CA2141431A1 (en)	1994-02-17
WO1994003072A1 (en)	1994-02-17
DE69333747T2 (de)	2005-12-29
FI950409A0 (fi)	1995-01-30
ES2235157T3 (es)	2005-07-01
DE69333747D1 (de)	2005-03-03
JPH09505201A (ja)	1997-05-27
FI950409L (fi)	1995-03-30
DK0655890T3 (da)	2005-06-06
EP0655890B1 (de)	2005-01-26

Legal Events

Date	Code	Title	Description
1995-08-03	GB	Transfer or assigment of application	Owner name: R!HM ENZYME FINLAND OY
1998-04-28	GB	Transfer or assigment of application	Owner name: R!HM ENZYME FINLAND OY
2005-07-27	PC	Transfer of assignment of patent	Owner name: AB ENZYMES GMBH Free format text: AB ENZYMES GMBH
2006-07-31	FG	Patent granted	Ref document number: 117202 Country of ref document: FI

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