FR2370792A1 - PERFECTED MICROSUPPORTS FOR CELL CULTURE, THEIR OBTAINING AND THEIR APPLICATIONS - Google Patents

PERFECTED MICROSUPPORTS FOR CELL CULTURE, THEIR OBTAINING AND THEIR APPLICATIONS

Info

Publication number
FR2370792A1
FR2370792A1 FR7733954A FR7733954A FR2370792A1 FR 2370792 A1 FR2370792 A1 FR 2370792A1 FR 7733954 A FR7733954 A FR 7733954A FR 7733954 A FR7733954 A FR 7733954A FR 2370792 A1 FR2370792 A1 FR 2370792A1
Authority
FR
France
Prior art keywords
microcarriers
produce
applications
microsupports
perfected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7733954A
Other languages
French (fr)
Other versions
FR2370792B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Publication of FR2370792A1 publication Critical patent/FR2370792A1/en
Application granted granted Critical
Publication of FR2370792B1 publication Critical patent/FR2370792B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention décrit des microsupports perfectionnés pour la croissance de cellules, des procédés pour produire ces microsupports et leurs applications. Ces microsupports ont des capacités de charge positive ajustées et/ou réglees dans un intervalle qui convient pour une bonne croissance des cellules. Pour produire de tels microsupports, on traite des perles (formées à partir de polymères comportant des groupes OH latéraux), comme des perles de dextrane, par une solution aqueuse d'une matière alcaline et d'une amine tertiaire chlorée ou bromée, dans des conditions précisément réglées pour produire la capacité d'échange voulue. Les microsupports à charge positive que l'on obtient ainsi ont servi à obtenir une croissance remarquable de cellules dépendant de leur fixation par ancrage. Ces cellules peuvent être récoltées elles-mêmes ou servir à la production de virus, de vaccins, d'hormones, etc.The invention describes improved microcarriers for cell growth, methods for producing these microcarriers and their applications. These microcarriers have positive charge capacities adjusted and / or regulated within a range which is suitable for good cell growth. To produce such microcarriers, beads (formed from polymers having side OH groups), such as dextran beads, are treated with an aqueous solution of an alkaline material and a chlorinated or brominated tertiary amine, in solutions. precisely tuned conditions to produce the desired exchange capacity. The positively charged microcarriers thus obtained served to achieve remarkable growth of cells dependent on their attachment by anchorage. These cells can be harvested on their own or used to produce viruses, vaccines, hormones, etc.

FR7733954A 1976-11-11 1977-11-10 PERFECTED MICROSUPPORTS FOR CELL CULTURE, THEIR OBTAINING AND THEIR APPLICATIONS Granted FR2370792A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US74099376A 1976-11-11 1976-11-11

Publications (2)

Publication Number Publication Date
FR2370792A1 true FR2370792A1 (en) 1978-06-09
FR2370792B1 FR2370792B1 (en) 1983-07-29

Family

ID=24978920

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7733954A Granted FR2370792A1 (en) 1976-11-11 1977-11-10 PERFECTED MICROSUPPORTS FOR CELL CULTURE, THEIR OBTAINING AND THEIR APPLICATIONS

Country Status (14)

Country Link
JP (3) JPS5362889A (en)
AU (1) AU508398B2 (en)
BE (1) BE860721A (en)
BR (1) BR7707551A (en)
CA (1) CA1063050A (en)
CH (1) CH615947A5 (en)
DE (2) DE2759579C2 (en)
FI (1) FI60407C (en)
FR (1) FR2370792A1 (en)
GB (1) GB1535150A (en)
IN (1) IN147612B (en)
NL (1) NL177926C (en)
SE (2) SE417108C (en)
ZA (1) ZA776251B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2470794A1 (en) * 1979-12-05 1981-06-12 Pasteur Institut NOVEL MICROPARTICLES, THEIR PREPARATION AND THEIR APPLICATIONS IN BIOLOGY, PARTICULARLY TO THE CULTURE OF HUMAN DIPLOID CELLS
FR2522678A1 (en) * 1982-03-03 1983-09-09 Kms Fusion Inc MICROSUPPORT WITH ITS GLASS SURFACE FOR GROWTH OF CELL CULTURE, AND PROCESS FOR ITS MANUFACTURE

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA776251B (en) * 1976-11-11 1978-07-26 Massachusetts Inst Technology Improved cell culture microcarriers
JPS592735U (en) * 1982-06-28 1984-01-09 シャープ株式会社 Remaining paper amount indicator in paper cassette
JPS60126453U (en) * 1984-02-02 1985-08-26 富士ゼロックス株式会社 Paper remaining amount display device such as facsimile
IL74259A0 (en) * 1984-02-06 1985-05-31 Surface Concepts Pty Ltd Improved method for cell culture
JPS60145139U (en) * 1984-03-07 1985-09-26 日本電信電話株式会社 Remaining amount display device for sheet recording paper
SE454518B (en) * 1984-05-21 1988-05-09 Statens Bakteriologiska Lab PROCEDURE FOR CULTURING DIPLOID CELLS IN THE PRESENTATION OF CELLULOSA FIBERS
JP2606213B2 (en) * 1986-04-22 1997-04-30 味の素株式会社 Complexes of Modified Microbial Cellulose with Gels and Animal Cell Membrane
DE3633891A1 (en) * 1986-10-04 1988-04-07 Akzo Gmbh METHOD AND DEVICE FOR CULTIVATING ANIMAL CELLS
US20140170748A1 (en) * 2012-12-14 2014-06-19 DePuy Synthes Products, LLC Nutrient Enriched Media for hUTC Growth
CN113249311B (en) * 2020-05-25 2022-06-28 北京唐颐惠康生物医学技术有限公司 Preparation method of degradable microcarrier for mesenchymal stem cell culture and its product and application

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1436323A (en) * 1972-04-26 1976-05-19 Wellcome Found Cell and virus culture systems
US3901095A (en) * 1974-06-17 1975-08-26 Joseph W Wechsler Bicycle gear shift
US3935067A (en) * 1974-11-22 1976-01-27 Wyo-Ben Products, Inc. Inorganic support for culture media
ZA776251B (en) * 1976-11-11 1978-07-26 Massachusetts Inst Technology Improved cell culture microcarriers
JPS5614270A (en) * 1979-07-14 1981-02-12 Ricoh Co Ltd Wet type development after treating device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2470794A1 (en) * 1979-12-05 1981-06-12 Pasteur Institut NOVEL MICROPARTICLES, THEIR PREPARATION AND THEIR APPLICATIONS IN BIOLOGY, PARTICULARLY TO THE CULTURE OF HUMAN DIPLOID CELLS
EP0030885A3 (en) * 1979-12-05 1981-07-15 Institut Pasteur Microparticles, their preparation and their biological uses, especially in the culture of diploid cells
FR2522678A1 (en) * 1982-03-03 1983-09-09 Kms Fusion Inc MICROSUPPORT WITH ITS GLASS SURFACE FOR GROWTH OF CELL CULTURE, AND PROCESS FOR ITS MANUFACTURE

Also Published As

Publication number Publication date
JPS5362889A (en) 1978-06-05
JPS5699790A (en) 1981-08-11
SE7712700L (en) 1978-05-12
CH615947A5 (en) 1980-02-29
NL7712202A (en) 1978-05-16
IN147612B (en) 1980-05-03
JPS5735953B2 (en) 1982-07-31
FR2370792B1 (en) 1983-07-29
BE860721A (en) 1978-03-01
BR7707551A (en) 1978-06-20
FI60407B (en) 1981-09-30
NL177926C (en) 1985-12-16
DE2749989A1 (en) 1978-05-18
AU3049177A (en) 1979-06-28
FI60407C (en) 1982-01-11
JPS5699789A (en) 1981-08-11
ZA776251B (en) 1978-07-26
AU508398B2 (en) 1980-03-20
CA1063050A (en) 1979-09-25
SE417108B (en) 1981-02-23
GB1535150A (en) 1978-12-06
NL177926B (en) 1985-07-16
JPS5614270B2 (en) 1981-04-03
DE2749989C3 (en) 1980-07-03
SE417108C (en) 1989-04-17
DE2749989B2 (en) 1979-10-25
SE460906B (en) 1989-12-04
FI773377A7 (en) 1978-05-12
DE2759579C2 (en) 1983-08-25
JPS575514B2 (en) 1982-01-30
SE8006993L (en) 1980-10-07

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