FR2393006A1 - Procede de purification de l'urokinase - Google Patents

Procede de purification de l'urokinase

Info

Publication number
FR2393006A1
FR2393006A1 FR7816192A FR7816192A FR2393006A1 FR 2393006 A1 FR2393006 A1 FR 2393006A1 FR 7816192 A FR7816192 A FR 7816192A FR 7816192 A FR7816192 A FR 7816192A FR 2393006 A1 FR2393006 A1 FR 2393006A1
Authority
FR
France
Prior art keywords
urokinase
solution containing
crude
urea
purified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7816192A
Other languages
English (en)
Other versions
FR2393006B1 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sumitomo Chemical Co Ltd
Original Assignee
Sumitomo Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sumitomo Chemical Co Ltd filed Critical Sumitomo Chemical Co Ltd
Publication of FR2393006A1 publication Critical patent/FR2393006A1/fr
Application granted granted Critical
Publication of FR2393006B1 publication Critical patent/FR2393006B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'urokinase est purifiée par la mise en contact d'une solution contenant de l'urokinase brute avec un échangeur de cations fortement acide qui contient un groupe acide sulfonique et est réglé sur le type NH 4 **+ afin d'adsorber l'urokinase sur cet échangeur de cations, puis par l'extraction de l'urokinase à partir de l'échangeur par élution. On ajoute au préalable de l'urée à la solution contenant l'urokinase brute dans une proportion telle que la concentration finale d'urée dans la solution se situe entre 3 et 8 M. Ce procédé est propre à fournir une urokinase purifiée qui présente une activité pratiquement constante et très spécifique, à partir d'une solution contenant de l'urokinase brute, avec un rendement élevé, quel que soit le degré de pureté de l'urokinase brute. L'urokinase est un enzyme qui est capable de produire la plasmine, enzyme fibrinolytique, à partir de plasminogène, et l'on sait que cette substance est non seulement utile pour le traitement de diverses sortes de thrombose, mais qu'elle est également capable de renforcer remarquablement le pouvoir des agents carcinostatiques lorsqu'elle est utilisée en association avec ces agents.
FR7816192A 1977-06-03 1978-05-31 Procede de purification de l'urokinase Granted FR2393006A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP52065975A JPS5935B2 (ja) 1977-06-03 1977-06-03 ウロキナ−ゼの精製法

Publications (2)

Publication Number Publication Date
FR2393006A1 true FR2393006A1 (fr) 1978-12-29
FR2393006B1 FR2393006B1 (fr) 1981-05-08

Family

ID=13302500

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7816192A Granted FR2393006A1 (fr) 1977-06-03 1978-05-31 Procede de purification de l'urokinase

Country Status (8)

Country Link
US (1) US4189350A (fr)
JP (1) JPS5935B2 (fr)
CA (1) CA1102263A (fr)
DE (1) DE2823353A1 (fr)
DK (1) DK147266C (fr)
FR (1) FR2393006A1 (fr)
GB (1) GB1556652A (fr)
IT (1) IT7849692A0 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988000615A1 (fr) * 1986-07-16 1988-01-28 Celltech Limited Procede de purification d'un activateur de plasminogene

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2121050B (en) * 1979-07-05 1986-03-26 Genentech Inc Preparation of functional human urokinase proteins
US5112755A (en) * 1982-04-15 1992-05-12 Genentech, Inc. Preparation of functional human urokinase proteins
JPS60183797U (ja) * 1984-05-17 1985-12-05 三菱重工業株式会社 フオ−クリフトの信号伝送装置
JPS6186400A (ja) * 1984-09-29 1986-05-01 新明和工業株式会社 高所作業車の操作情報制御装置
DE3439980A1 (de) * 1984-11-02 1986-05-07 Behringwerke Ag, 3550 Marburg Verfahren zur reinigung sowie pasteurisierung von urokinase
JPS61115492A (ja) * 1984-11-07 1986-06-03 Green Cross Corp:The ウロキナ−ゼの精製方法
KR100419448B1 (ko) * 2001-03-09 2004-02-19 주)녹십자 고정화 유로키나아제 칼럼의 재생방법
US11982045B2 (en) * 2022-05-11 2024-05-14 Imam Abdulrahman Bin Faisal University System and methods for recycling heat and water in a steam press machine

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1289809B (de) * 1965-10-01 1969-02-27 Green Cross Corp Verfahren zur Gewinnung von Urokinase fuer Injektionszwecke
JPS5212984A (en) * 1975-07-22 1977-01-31 Kanebo Ltd Purification of urokinase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3957582A (en) * 1974-11-20 1976-05-18 Abbott Laboratories Purification of urokinase
JPS51123885A (en) * 1975-04-18 1976-10-28 Hitachi Chem Co Ltd Process for purifying urokinase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1289809B (de) * 1965-10-01 1969-02-27 Green Cross Corp Verfahren zur Gewinnung von Urokinase fuer Injektionszwecke
JPS5212984A (en) * 1975-07-22 1977-01-31 Kanebo Ltd Purification of urokinase

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CA1975 *
CA1977 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988000615A1 (fr) * 1986-07-16 1988-01-28 Celltech Limited Procede de purification d'un activateur de plasminogene

Also Published As

Publication number Publication date
DE2823353A1 (de) 1978-12-21
JPS542396A (en) 1979-01-09
US4189350A (en) 1980-02-19
IT7849692A0 (it) 1978-06-02
FR2393006B1 (fr) 1981-05-08
JPS5935B2 (ja) 1984-01-05
DK147266B (da) 1984-05-28
CA1102263A (fr) 1981-06-02
DK147266C (da) 1984-12-10
GB1556652A (en) 1979-11-28
DK248678A (da) 1978-12-04

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Legal Events

Date Code Title Description
ST Notification of lapse