FR2422720A2 - Separation et purification de proteines par chromatographie - Google Patents

Separation et purification de proteines par chromatographie

Info

Publication number
FR2422720A2
FR2422720A2 FR7810769A FR7810769A FR2422720A2 FR 2422720 A2 FR2422720 A2 FR 2422720A2 FR 7810769 A FR7810769 A FR 7810769A FR 7810769 A FR7810769 A FR 7810769A FR 2422720 A2 FR2422720 A2 FR 2422720A2
Authority
FR
France
Prior art keywords
chromatography
purification
protein separation
separation
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7810769A
Other languages
English (en)
Other versions
FR2422720B2 (fr
Inventor
Charles Dulout
Andre Peyrouset
Rene Panaris
Claude Hannoun
Jean Vincent
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Societe National Elf Aquitaine
Societe Nationale Elf Aquitaine Production SA
Original Assignee
Societe National Elf Aquitaine
Societe Nationale Elf Aquitaine Production SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR7712518A external-priority patent/FR2388585A1/fr
Application filed by Societe National Elf Aquitaine, Societe Nationale Elf Aquitaine Production SA filed Critical Societe National Elf Aquitaine
Priority to FR7810769A priority Critical patent/FR2422720A2/fr
Priority to DE19782817871 priority patent/DE2817871A1/de
Priority to CA301,897A priority patent/CA1111415A/fr
Priority to GB16321/78A priority patent/GB1595378A/en
Priority to JP4916878A priority patent/JPS53140097A/ja
Priority to ES469156A priority patent/ES469156A1/es
Priority to SU782614452A priority patent/SU784784A3/ru
Priority to US05/900,081 priority patent/US4199450A/en
Publication of FR2422720A2 publication Critical patent/FR2422720A2/fr
Publication of FR2422720B2 publication Critical patent/FR2422720B2/fr
Application granted granted Critical
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/283—Porous sorbents based on silica
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/3085—Chemical treatments not covered by groups B01J20/3007 - B01J20/3078
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/826—Viruses

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Perfectionnement à la séparation de protéines, en particulier de virus, par des moyens physiques en présence d'un agent antiseptique, constitué par un ou plusieurs hydrocarbures inférieurs, halogénés (chloroforme particulièrement) ; la proportion de ces agents utilisés dans la solution à purifier est comprise dans la limite de solubilité de ces agents dans l'eau. Perfectionnement particulièrement intéressant dans la séparation des protéines par chromatogrqhie.
FR7810769A 1977-04-26 1978-04-12 Separation et purification de proteines par chromatographie Granted FR2422720A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FR7810769A FR2422720A2 (fr) 1977-04-26 1978-04-12 Separation et purification de proteines par chromatographie
DE19782817871 DE2817871A1 (de) 1977-04-26 1978-04-24 Verfahren und vorrichtung zur abtrennung und reinigung von proteinen durch chromatographie
JP4916878A JPS53140097A (en) 1977-04-26 1978-04-25 Separation and purification of protein by chromatography
GB16321/78A GB1595378A (en) 1977-04-26 1978-04-25 Chromatographic separation and purification of proteins and viruses
CA301,897A CA1111415A (fr) 1977-04-26 1978-04-25 Separation et purification de proteines par chromatographie
ES469156A ES469156A1 (es) 1977-04-26 1978-04-26 Procedimiento y dispositivo de separacion y purificacion de proteinas por cromatografia
SU782614452A SU784784A3 (ru) 1977-04-26 1978-04-26 Способ выделени протеина
US05/900,081 US4199450A (en) 1977-04-26 1978-04-26 Process of separating from an aqueous medium a protein or a morphologically organized unit by liquid exclusion chromatography

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR7712518A FR2388585A1 (fr) 1977-04-26 1977-04-26 Separation et purification de proteines et/ou d'unites morphologiquement organisees (virus) par la chromatographie d'exclusion
FR7810769A FR2422720A2 (fr) 1977-04-26 1978-04-12 Separation et purification de proteines par chromatographie

Publications (2)

Publication Number Publication Date
FR2422720A2 true FR2422720A2 (fr) 1979-11-09
FR2422720B2 FR2422720B2 (fr) 1983-02-04

Family

ID=26219979

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7810769A Granted FR2422720A2 (fr) 1977-04-26 1978-04-12 Separation et purification de proteines par chromatographie

Country Status (8)

Country Link
US (1) US4199450A (fr)
JP (1) JPS53140097A (fr)
CA (1) CA1111415A (fr)
DE (1) DE2817871A1 (fr)
ES (1) ES469156A1 (fr)
FR (1) FR2422720A2 (fr)
GB (1) GB1595378A (fr)
SU (1) SU784784A3 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2475572A1 (fr) * 1980-02-11 1981-08-14 Pasteur Institut Procede pour l'obtention de fragments de virus a enveloppe lipidique, en particulier d'antigenes utilisables comme vaccins, produits obtenus et applications
DE3005495A1 (de) * 1980-02-14 1981-08-20 Pasteur Institut Verfahren zur herstellung von fragmenten von viren mit lipidhuelle und sie enthaltende arzneimittel
US4344935A (en) 1980-06-05 1982-08-17 Synthelabo Process for the isolation of viral glycoproteic antigens and its application to the preparation of vaccines

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5760261A (en) * 1980-09-29 1982-04-12 Sekisui Chem Co Ltd Manufacture of filler for liquid chromatograph
JPS58223062A (ja) * 1982-06-21 1983-12-24 Toyo Soda Mfg Co Ltd 生理活性物質及び／又は薬物の分離分析方法
US4468331A (en) * 1982-09-13 1984-08-28 E. I. Du Pont De Nemours And Company Method and system for liquid choromatography separations
HU192642B (en) * 1984-12-19 1987-06-29 Forte Fotokemiai Ipar Process for the extraction of nucleinic acids and purine bases from gelatine
WO1994000214A1 (fr) * 1992-06-19 1994-01-06 Sepracor Inc. Supports poreux stabilises et rendus passifs, et leur procede de preparation et d'utilisation
US5451660A (en) * 1993-12-13 1995-09-19 Genentech, Inc. Method for purifying polypeptides
DE19926041A1 (de) * 1999-06-08 2000-12-21 Octapharma Ag Lachen Verfahren zur Entfernung oder Abtrennung von Viren in potentiell Viren enthaltenden Proben
DE19964015A1 (de) * 1999-12-30 2001-08-09 Octapharma Ag Lachen Verfahren zur Entfernung oder Abtrennung von Viren in potentiell Viren enthaltenden Proben
SG190031A1 (en) * 2010-10-27 2013-06-28 Philip Morris Prod Methods for capturing virus like particles from plants using expanded bed chromatography
WO2014032285A1 (fr) * 2012-08-31 2014-03-06 西安奥岚科技开发有限责任公司 Procédé et système de séparation chromatographique en phase liquide multidimensionnelle mis en oeuvre dans la séparation de protéines
RU2015114330A (ru) 2012-09-17 2016-11-10 У.Р. Грейс Энд Ко.-Конн. Хроматографические среды и устройства
JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 官能化担体材料並びに官能化担体材料を作製及び使用する方法
CN107921407B (zh) 2015-06-05 2022-07-05 格雷斯公司 生物处理吸附澄清剂及其制备和使用方法
CN114894946B (zh) * 2022-06-20 2023-07-18 山东宏济堂制药集团股份有限公司 一种抗流感、解热抗炎的中药组合物的质量控制方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2103164A5 (fr) * 1970-07-23 1972-04-07 Ciba Geigy Ag
CA987230A (en) * 1972-12-05 1976-04-13 Institut Armand-Frappier Process for the filtration of a suspension containing a protein such as an influenza virus vaccine

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3549524A (en) * 1965-11-10 1970-12-22 Wolfgang Haller Material and method for performing steric separations
SE334862B (fr) * 1966-04-14 1971-05-10 Pharmacia Ab
SE333824B (fr) * 1966-06-22 1971-03-29 Pharmacia Ab
US3701609A (en) * 1971-05-13 1972-10-31 David G Bailey Apparatus for automatically adding preselected patterns of eluent solutions to a chromatographic column and monitoring and collecting eluted fractions
US3926800A (en) * 1974-01-25 1975-12-16 Beckman Instruments Inc Temperature stabilized water jacket chromatographic column
US4123931A (en) * 1976-08-03 1978-11-07 The Dow Chemical Company Method for uniformly coating liquid phases on glass beads and applications thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2103164A5 (fr) * 1970-07-23 1972-04-07 Ciba Geigy Ag
CA987230A (en) * 1972-12-05 1976-04-13 Institut Armand-Frappier Process for the filtration of a suspension containing a protein such as an influenza virus vaccine

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2475572A1 (fr) * 1980-02-11 1981-08-14 Pasteur Institut Procede pour l'obtention de fragments de virus a enveloppe lipidique, en particulier d'antigenes utilisables comme vaccins, produits obtenus et applications
US4522809A (en) * 1980-02-11 1985-06-11 Institut Pasteur Process for obtaining lipid envelope virus sub-units, notably antigens for use as vaccines, the products obtained and their applications
DE3005495A1 (de) * 1980-02-14 1981-08-20 Pasteur Institut Verfahren zur herstellung von fragmenten von viren mit lipidhuelle und sie enthaltende arzneimittel
US4344935A (en) 1980-06-05 1982-08-17 Synthelabo Process for the isolation of viral glycoproteic antigens and its application to the preparation of vaccines

Also Published As

Publication number Publication date
ES469156A1 (es) 1979-11-01
FR2422720B2 (fr) 1983-02-04
CA1111415A (fr) 1981-10-27
GB1595378A (en) 1981-08-12
DE2817871A1 (de) 1978-12-07
SU784784A3 (ru) 1980-11-30
US4199450A (en) 1980-04-22
JPS53140097A (en) 1978-12-06

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