FR2501865B2 - Procede d'analyse, par sorption differentielle d'un colorant metachromatique, des divers leucocytes du sang - Google Patents

Procede d'analyse, par sorption differentielle d'un colorant metachromatique, des divers leucocytes du sang

Info

Publication number
FR2501865B2
FR2501865B2 FR8204053A FR8204053A FR2501865B2 FR 2501865 B2 FR2501865 B2 FR 2501865B2 FR 8204053 A FR8204053 A FR 8204053A FR 8204053 A FR8204053 A FR 8204053A FR 2501865 B2 FR2501865 B2 FR 2501865B2
Authority
FR
France
Prior art keywords
analysis
blood leukocytes
various blood
metachromatic dye
differential sorption
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
FR8204053A
Other languages
English (en)
Other versions
FR2501865A2 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of FR2501865A2 publication Critical patent/FR2501865A2/fr
Application granted granted Critical
Publication of FR2501865B2 publication Critical patent/FR2501865B2/fr
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Ecology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
FR8204053A 1981-03-11 1982-03-10 Procede d'analyse, par sorption differentielle d'un colorant metachromatique, des divers leucocytes du sang Expired FR2501865B2 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/242,662 US4400370A (en) 1980-03-12 1981-03-11 Metachromatic dye sorption means for differential determination of leukocytes

Publications (2)

Publication Number Publication Date
FR2501865A2 FR2501865A2 (fr) 1982-09-17
FR2501865B2 true FR2501865B2 (fr) 1985-11-15

Family

ID=22915693

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8204053A Expired FR2501865B2 (fr) 1981-03-11 1982-03-10 Procede d'analyse, par sorption differentielle d'un colorant metachromatique, des divers leucocytes du sang

Country Status (7)

Country Link
US (1) US4400370A (fr)
JP (1) JPS57161550A (fr)
CA (1) CA1191079A (fr)
DE (1) DE3208629A1 (fr)
FR (1) FR2501865B2 (fr)
GB (1) GB2095402B (fr)
IT (1) IT1148123B (fr)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4615878A (en) * 1980-03-12 1986-10-07 Lawrence Kass Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes
US4581223A (en) * 1980-03-12 1986-04-08 Lawrence Kass Individual leukocyte determination by means of differential metachromatic dye sorption
US4500509A (en) * 1981-03-11 1985-02-19 Lawrence Kass Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes
DE3238353A1 (de) * 1982-10-15 1984-04-19 Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Verfahren zur simultanen quantitativen bestimmung der blutzellen und reagenz hierfuer
US4508821A (en) * 1983-07-05 1985-04-02 Becton Dickinson And Company Detection of white cell associated bacteria with a fluorescent dye
WO1985005180A1 (fr) * 1984-04-27 1985-11-21 Merck Patent Gmbh Identification de myeloblastes et d'autres cellules granulocytiques immatures
DE3574408D1 (en) * 1984-05-15 1989-12-28 Lawrence Kass Single dye replacement for romanowsky plural dye variable compositions in human biopsy specimen constituent identifications
US4714606A (en) * 1984-05-15 1987-12-22 Cytocolor Incorporated Method of staining and identifying cells and compositions thereof
WO1986001293A1 (fr) * 1984-08-09 1986-02-27 Lawrence Kass Differenciation, identification et enumeration de neutrophiles, d'erythroblastes et de sous-populations de lymphocytes avec du bleu basique 141
US4810487A (en) * 1986-01-02 1989-03-07 Cytocolor, Inc. Method of identifying polymethine stained lymphocyte subpopulations and compositions thereof
JPH076979B2 (ja) * 1986-09-10 1995-01-30 東亜医用電子株式会社 フロ−サイトメトリ−による白血球分類に使用される試薬
US5175109A (en) * 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
US4783401A (en) * 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
US5179026A (en) * 1986-11-27 1993-01-12 Toa Medical Electronics Co., Ltd. Method of classifying leukocytes by flow cytometry and reagents used in the method
US5039613A (en) * 1986-11-27 1991-08-13 Toa Medical Electronics Co., Ltd. Reagents used in a method of classifying leukocytes by flow cytometry
US4882284A (en) * 1987-04-13 1989-11-21 Ortho Pharmaceutical Corporation Method for quantitating and differentiating white blood cells
DE68927479T2 (de) * 1988-05-02 1997-04-03 Phanos Tech Inc Verbindungen, zusammensetzungen und verfahren zum binden von bio-affektions-substanzen an oberflächenmembranen von bioteilchen
US5407794A (en) * 1992-09-08 1995-04-18 Cytocolor Inc. Oxazine stained lymphocytes and method
DE4309328C2 (de) * 1993-03-18 1998-03-12 Volker Ost Verfahren zur Differenzierung, Konzentrationsbestimmung und Sortierung von Erythrozyten, Thrombozyten und Leukozyten
US6136540A (en) 1994-10-03 2000-10-24 Ikonisys Inc. Automated fluorescence in situ hybridization detection of genetic abnormalities
US5352613A (en) * 1993-10-07 1994-10-04 Tafas Triantafillos P Cytological screening method
ES2156927T3 (es) * 1994-10-20 2001-08-01 Sysmex Corp Reactivo y metodo para analizar componentes solidos en orina.
US6235536B1 (en) * 1998-03-07 2001-05-22 Robert A. Levine Analysis of quiescent anticoagulated whole blood samples
US6197593B1 (en) 1998-10-20 2001-03-06 Coulter International Corp. Method for enumerating blood cells
CA2441878A1 (fr) * 2001-03-19 2002-09-26 Ikonisys, Inc. Systeme et procede pour accentuer le contraste d'une image produite par un microscope a epifluorescence
CN103977848B (zh) 2007-04-06 2016-08-24 加利福尼亚技术学院 微流体装置
EP2331992A4 (fr) 2008-10-02 2014-12-17 Pixcell Medical Technologies Ltd Imagerie optique fondée sur la focalisation viscoélastique
EP2450706B1 (fr) 2009-07-03 2017-07-26 Sysmex Corporation Analyseur de sang et procédé d'analyse de sang
WO2013082029A1 (fr) 2011-11-28 2013-06-06 California Institute Of Technology Compositions et procédés pour le comptage différentiel des leucocytes
US20140170678A1 (en) 2012-12-17 2014-06-19 Leukodx Ltd. Kits, compositions and methods for detecting a biological condition
US10610861B2 (en) 2012-12-17 2020-04-07 Accellix Ltd. Systems, compositions and methods for detecting a biological condition
EP3508848B1 (fr) 2012-12-17 2022-10-05 Accellix Ltd Systèmes et procédés pour déterminer un état chimique

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE403179A (fr) * 1933-05-16
JPS5239055B1 (fr) * 1966-05-24 1977-10-03
US3617185A (en) * 1968-08-19 1971-11-02 Gaf Corp Stable highly concentrated solutions of basic dyes
DE2153473A1 (de) * 1971-10-27 1973-05-03 Reflecta G Junge Filmleuchte
US3883247A (en) * 1973-10-30 1975-05-13 Bio Physics Systems Inc Method for fluorescence analysis of white blood cells
US3985500A (en) * 1974-08-21 1976-10-12 Gaf Corporation Black dye formulation
DE2515966C3 (de) * 1975-04-11 1979-11-22 Boehringer Mannheim Gmbh, 6800 Mannheim Vorgefärbte Objektträger für die Blutunte rsuchung
FR2315251A1 (fr) * 1975-06-27 1977-01-21 Inst Nat Sante Rech Med Procede et composition metachromatique pour la numeration des leucocytes, plus particulierement des basophiles
DE2962866D1 (en) * 1978-03-09 1982-07-08 Merck Patent Gmbh Method for determining leukaemic cells
US4581223A (en) * 1980-03-12 1986-04-08 Lawrence Kass Individual leukocyte determination by means of differential metachromatic dye sorption

Also Published As

Publication number Publication date
JPH0415416B2 (fr) 1992-03-17
GB2095402B (en) 1984-10-10
US4400370A (en) 1983-08-23
DE3208629A1 (de) 1982-09-23
DE3208629C2 (fr) 1992-06-11
IT8247950A0 (it) 1982-03-09
GB2095402A (en) 1982-09-29
CA1191079A (fr) 1985-07-30
IT1148123B (it) 1986-11-26
JPS57161550A (en) 1982-10-05
FR2501865A2 (fr) 1982-09-17

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