FR2955334B1 - Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux - Google Patents

Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux

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Publication number
FR2955334B1
FR2955334B1 FR1150365A FR1150365A FR2955334B1 FR 2955334 B1 FR2955334 B1 FR 2955334B1 FR 1150365 A FR1150365 A FR 1150365A FR 1150365 A FR1150365 A FR 1150365A FR 2955334 B1 FR2955334 B1 FR 2955334B1
Authority
FR
France
Prior art keywords
support
cell culture
matrix
regeneration
cartilaginous tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1150365A
Other languages
English (en)
Other versions
FR2955334A1 (fr
Inventor
Dominique Vacher
Florence Heraud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ibsa Pharma Sas Fr
Original Assignee
Laboratoires Genevrier SAS
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Filing date
Publication date
Application filed by Laboratoires Genevrier SAS filed Critical Laboratoires Genevrier SAS
Priority to FR1150365A priority Critical patent/FR2955334B1/fr
Publication of FR2955334A1 publication Critical patent/FR2955334A1/fr
Application granted granted Critical
Publication of FR2955334B1 publication Critical patent/FR2955334B1/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)

Abstract

L'invention concerne l'utilisation d'une matrice de collagène réticulée poreuse, principalement composée de collagène de type II et de glycosaminoglycanes, et présentant une porosité ouverte sur toute ou la majeure partie de sa surface, comme support à la culture cellulaire ex vivo de chondrocytes, ainsi que sur le biomatériau obtenu par cette culture. Ce biomatériau est destiné à être implanté pour réparer les défauts localisés du cartilage.
FR1150365A 2010-01-15 2011-01-17 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux Active FR2955334B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR1150365A FR2955334B1 (fr) 2010-01-15 2011-01-17 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1000152A FR2955333B1 (fr) 2010-01-15 2010-01-15 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux;
FR1150365A FR2955334B1 (fr) 2010-01-15 2011-01-17 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux

Publications (2)

Publication Number Publication Date
FR2955334A1 FR2955334A1 (fr) 2011-07-22
FR2955334B1 true FR2955334B1 (fr) 2013-09-06

Family

ID=42340603

Family Applications (2)

Application Number Title Priority Date Filing Date
FR1000152A Active FR2955333B1 (fr) 2010-01-15 2010-01-15 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux;
FR1150365A Active FR2955334B1 (fr) 2010-01-15 2011-01-17 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux

Family Applications Before (1)

Application Number Title Priority Date Filing Date
FR1000152A Active FR2955333B1 (fr) 2010-01-15 2010-01-15 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux;

Country Status (1)

Country Link
FR (2) FR2955333B1 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2616318A1 (fr) * 1987-06-15 1988-12-16 Centre Nat Rech Scient Peau artificielle et son procede de preparation
GB9721585D0 (en) * 1997-10-10 1997-12-10 Geistlich Soehne Ag Chemical product
EP1254670A1 (fr) * 2001-05-03 2002-11-06 Stichting Katholieke Universiteit Matrices de collagène II pour la régéneration de cartilage

Also Published As

Publication number Publication date
FR2955333A1 (fr) 2011-07-22
FR2955334A1 (fr) 2011-07-22
FR2955333B1 (fr) 2014-03-21

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