FR3068366B1 - Procede de production de progeniteurs erythroides - Google Patents

Procede de production de progeniteurs erythroides Download PDF

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Publication number
FR3068366B1
FR3068366B1 FR1756222A FR1756222A FR3068366B1 FR 3068366 B1 FR3068366 B1 FR 3068366B1 FR 1756222 A FR1756222 A FR 1756222A FR 1756222 A FR1756222 A FR 1756222A FR 3068366 B1 FR3068366 B1 FR 3068366B1
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FR
France
Prior art keywords
erythroid progenitors
producing erythroid
producing
bringing
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1756222A
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English (en)
Other versions
FR3068366A1 (fr
Inventor
Laurence Guyonneau-Harmand
Loic Garcon
Frédéric Aurade
Nicolas Rebergue
Frédéric Relaix
Luc Douay
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Santé et de la Recherche Médicale
Établissement Français du Sang
Sorbonne Université
Original Assignee
Université Pierre et Marie Curie
Institut National de la Santé et de la Recherche Médicale
Établissement Français du Sang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR1756222A priority Critical patent/FR3068366B1/fr
Application filed by Université Pierre et Marie Curie, Institut National de la Santé et de la Recherche Médicale, Établissement Français du Sang filed Critical Université Pierre et Marie Curie
Priority to KR1020207002966A priority patent/KR102744512B1/ko
Priority to JP2019572440A priority patent/JP7162625B2/ja
Priority to EP18733656.5A priority patent/EP3645707A1/fr
Priority to AU2018293389A priority patent/AU2018293389B2/en
Priority to CN201880044213.0A priority patent/CN111373029B/zh
Priority to CA3067551A priority patent/CA3067551A1/fr
Priority to US16/627,396 priority patent/US20200370015A1/en
Priority to PCT/EP2018/067833 priority patent/WO2019002625A1/fr
Publication of FR3068366A1 publication Critical patent/FR3068366A1/fr
Application granted granted Critical
Publication of FR3068366B1 publication Critical patent/FR3068366B1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0641—Erythrocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/45—Artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé de production in vitro de progéniteurs érythroïdes comprenant la mise en contact de cellules souches hématopoïétiques, génétiquement modifiées ou non, avec un milieu de culture cellulaire défini comprenant une hormone glucocorticoïde et un inducteur d'autophagie.
FR1756222A 2017-06-30 2017-06-30 Procede de production de progeniteurs erythroides Active FR3068366B1 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
FR1756222A FR3068366B1 (fr) 2017-06-30 2017-06-30 Procede de production de progeniteurs erythroides
JP2019572440A JP7162625B2 (ja) 2017-06-30 2018-07-02 赤血球系前駆細胞を作製する方法
EP18733656.5A EP3645707A1 (fr) 2017-06-30 2018-07-02 Procédé de production de progéniteurs érythroïdes
AU2018293389A AU2018293389B2 (en) 2017-06-30 2018-07-02 Method for producing erythroid progenitor cells
KR1020207002966A KR102744512B1 (ko) 2017-06-30 2018-07-02 적혈구 전구체 세포의 생산 방법
CN201880044213.0A CN111373029B (zh) 2017-06-30 2018-07-02 产生红系祖细胞的方法
CA3067551A CA3067551A1 (fr) 2017-06-30 2018-07-02 Procede de production de progeniteurs erythroides
US16/627,396 US20200370015A1 (en) 2017-06-30 2018-07-02 Method for producing erythroid progenitor cells
PCT/EP2018/067833 WO2019002625A1 (fr) 2017-06-30 2018-07-02 Procédé de production de progéniteurs érythroïdes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1756222 2017-06-30
FR1756222A FR3068366B1 (fr) 2017-06-30 2017-06-30 Procede de production de progeniteurs erythroides

Publications (2)

Publication Number Publication Date
FR3068366A1 FR3068366A1 (fr) 2019-01-04
FR3068366B1 true FR3068366B1 (fr) 2023-11-24

Family

ID=60450747

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1756222A Active FR3068366B1 (fr) 2017-06-30 2017-06-30 Procede de production de progeniteurs erythroides

Country Status (9)

Country Link
US (1) US20200370015A1 (fr)
EP (1) EP3645707A1 (fr)
JP (1) JP7162625B2 (fr)
KR (1) KR102744512B1 (fr)
CN (1) CN111373029B (fr)
AU (1) AU2018293389B2 (fr)
CA (1) CA3067551A1 (fr)
FR (1) FR3068366B1 (fr)
WO (1) WO2019002625A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7194030B2 (ja) * 2019-01-30 2022-12-21 株式会社エビデント 赤血球モニタリング装置
CN116568802A (zh) * 2020-10-30 2023-08-08 西湖生物医药科技(上海)有限公司 制备成熟红细胞的体外方法
EP4365283A4 (fr) * 2021-06-30 2025-06-11 National University Corporation Chiba University Procédé d'amélioration des propriétés prolifératives de cellules myéloïdes progénitrices communes (cmp) ou de cellules progénitrices myélocytiques
WO2024010317A1 (fr) * 2022-07-04 2024-01-11 주식회사 아트블러드 Établissement d'une lignée cellulaire progénitrice érythroïde immortalisée ayant une capacité supérieure de différenciation en globules rouges par l'utilisation d'une combinaison de surexpression génétique, son procédé de préparation et son utilisation
WO2024018018A1 (fr) * 2022-07-21 2024-01-25 Etablissement Francais Du Sang Utilisation d'un composé vitisine pour la production de cellules hématopoïétiques
KR20240167593A (ko) * 2023-05-18 2024-11-27 주식회사 레드진 적혈구 생산용 형질전환 세포 및 이의 용도
CN116640728B (zh) * 2023-07-24 2023-10-20 呈诺再生医学科技(北京)有限公司 RO8191和AS2863619在诱导产生表达成人型β珠蛋白的脱核红细胞中的应用
KR20260060511A (ko) * 2024-10-24 2026-05-06 부산대학교 산학협력단 Ut2 유전자 결손 적혈구 전구세포 및 이의 적혈구 분화 방법
WO2026092339A1 (fr) * 2024-10-28 2026-05-07 苏州血霁生物科技有限公司 Procédé pour la prolifération et la différenciation des cellules mkp et utilisation associée
WO2026132339A1 (fr) 2024-12-19 2026-06-25 Etablissement Francais Du Sang Procédés et supports pour la production de cellules hématopoïétiques

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883507B2 (en) * 2005-10-18 2014-11-11 The Regents Of The University Of Colorado Conditionally immortalized long-term hematopoietic stem cells and methods of making and using such cells
WO2007056423A1 (fr) 2005-11-07 2007-05-18 The General Hospital Corporation Procedes et compositions de modulation du vieillissement des cellules souches
CN101045914A (zh) 2006-03-29 2007-10-03 中国人民解放军军事医学科学院野战输血研究所 体外诱导造血干/祖细胞分化为成熟红细胞的方法与应用
DK2313496T3 (en) 2008-07-21 2014-12-15 Taiga Biotechnologies Inc Anukleære differentiated cells, and method for preparation thereof
US20120252697A1 (en) 2009-09-01 2012-10-04 Mcmaster University Transformed human pluripotent stem cells and associated methods
BR122013008030B8 (pt) 2009-09-15 2021-07-27 Univ Tokyo método para produzir células eritroides e método para produzir um produto sanguíneo
PL2539441T3 (pl) 2010-02-22 2017-06-30 Université Pierre Et Marie Curie (Paris 6) Podłoże do hodowli komórkowej do wzrostu i różnicowania komórek linii hematopoetycznych
EP2832851B1 (fr) * 2012-03-28 2019-04-17 Quarrymen & Co. Inc. Cellules souches immortalisées et composition médicinale et préparation médicinale comprenant un produit associé en tant que principe actif
JP2016135100A (ja) 2013-05-07 2016-07-28 学校法人金沢医科大学 赤芽球の脱核方法及び脱核赤血球の維持方法
CN111876387A (zh) 2013-05-15 2020-11-03 罗切斯特大学 人广泛自我更新型成红细胞(esre)
US11519901B2 (en) 2015-04-03 2022-12-06 The University Of Tokyo Method for screening for cancer therapeutic agent
US11242375B2 (en) * 2015-09-04 2022-02-08 Memorial Sloan Kettering Cancer Center Immune cell compositions and methods of use

Also Published As

Publication number Publication date
JP7162625B2 (ja) 2022-10-28
AU2018293389B2 (en) 2024-10-10
CA3067551A1 (fr) 2019-01-03
CN111373029A (zh) 2020-07-03
CN111373029B (zh) 2025-01-03
AU2018293389A1 (en) 2020-01-16
WO2019002625A1 (fr) 2019-01-03
EP3645707A1 (fr) 2020-05-06
KR102744512B1 (ko) 2024-12-19
FR3068366A1 (fr) 2019-01-04
KR20200060343A (ko) 2020-05-29
JP2020525031A (ja) 2020-08-27
US20200370015A1 (en) 2020-11-26

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