FR3076294B1 - Procede de purification d'anticorps a partir de lait brut - Google Patents

Procede de purification d'anticorps a partir de lait brut Download PDF

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Publication number
FR3076294B1
FR3076294B1 FR1763368A FR1763368A FR3076294B1 FR 3076294 B1 FR3076294 B1 FR 3076294B1 FR 1763368 A FR1763368 A FR 1763368A FR 1763368 A FR1763368 A FR 1763368A FR 3076294 B1 FR3076294 B1 FR 3076294B1
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France
Prior art keywords
raw milk
purifying antibodies
milk
filtration
depth filter
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FR1763368A
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English (en)
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FR3076294A1 (fr
Inventor
Philippe Paolantonacci
Beatrice Claudel
Abdessatar Chtourou
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LFB SA
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LFB SA
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Publication date
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Priority to FR1763368A priority Critical patent/FR3076294B1/fr
Priority to EP18830873.8A priority patent/EP3732188A1/fr
Priority to PCT/EP2018/097077 priority patent/WO2019129848A1/fr
Priority to US16/956,845 priority patent/US20210024616A1/en
Publication of FR3076294A1 publication Critical patent/FR3076294A1/fr
Application granted granted Critical
Publication of FR3076294B1 publication Critical patent/FR3076294B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/04—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from milk
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente demande concerne un procédé de préparation d'une composition d'anticorps à partir de lait brut d'un mammifère non humain exprimant ledit anticorps dans son lait, comprenant les étapes a) de précipitation du lait brut à l'acide caprylique, b) de séparation consistant en une centrifugation ou une filtration à travers un filtre en profondeur, et optionnellement c) de filtration à travers un filtre en profondeur à charbon actif.
FR1763368A 2017-12-29 2017-12-29 Procede de purification d'anticorps a partir de lait brut Active FR3076294B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR1763368A FR3076294B1 (fr) 2017-12-29 2017-12-29 Procede de purification d'anticorps a partir de lait brut
EP18830873.8A EP3732188A1 (fr) 2017-12-29 2018-12-28 Procédé de purification d'anticorps à partir de lait brut
PCT/EP2018/097077 WO2019129848A1 (fr) 2017-12-29 2018-12-28 Procédé de purification d'anticorps à partir de lait brut
US16/956,845 US20210024616A1 (en) 2017-12-29 2018-12-28 Method for Purifying Antibodies from Raw Milk

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1763368A FR3076294B1 (fr) 2017-12-29 2017-12-29 Procede de purification d'anticorps a partir de lait brut
FR1763368 2017-12-29

Publications (2)

Publication Number Publication Date
FR3076294A1 FR3076294A1 (fr) 2019-07-05
FR3076294B1 true FR3076294B1 (fr) 2022-01-28

Family

ID=62528506

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1763368A Active FR3076294B1 (fr) 2017-12-29 2017-12-29 Procede de purification d'anticorps a partir de lait brut

Country Status (4)

Country Link
US (1) US20210024616A1 (fr)
EP (1) EP3732188A1 (fr)
FR (1) FR3076294B1 (fr)
WO (1) WO2019129848A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024110444A1 (fr) 2022-11-21 2024-05-30 Chreto Aps Procédé de purification d'un produit cible à l'aide d'une technologie de purification par affinité

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* Cited by examiner, † Cited by third party
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US3053869A (en) 1959-12-31 1962-09-11 Standard Oil Co Carboxylic acids
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US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5827690A (en) 1993-12-20 1998-10-27 Genzyme Transgenics Corporatiion Transgenic production of antibodies in milk
EP0690452A3 (fr) 1994-06-28 1999-01-07 Advanced Micro Devices, Inc. Mémoire électriquement effaçable et procédé d'effacement
AU7256596A (en) 1995-10-05 1997-04-28 Immucell Corporation Process for isolating immunoglobulins in whey
IL138608A0 (en) 1998-04-02 2001-10-31 Genentech Inc Antibody variants and fragments thereof
WO2000026357A2 (fr) 1998-11-02 2000-05-11 Genzyme Transgenic Corp. Mammiferes transgeniques et clones
KR100940380B1 (ko) 1999-01-15 2010-02-02 제넨테크, 인크. 효과기 기능이 변화된 폴리펩티드 변이체
EP1220606A4 (fr) 1999-10-14 2004-04-14 Gtc Biotherapeutics Inc Methodes de production d'une molecule cible chez un animal transgenique et de purification de la molecule cible
JP4336498B2 (ja) 2000-12-12 2009-09-30 メディミューン，エルエルシー 延長した半減期を有する分子ならびにその組成物および用途
US20040002587A1 (en) 2002-02-20 2004-01-01 Watkins Jeffry D. Fc region variants
WO2004029207A2 (fr) 2002-09-27 2004-04-08 Xencor Inc. Variants fc optimises et methodes destinees a leur generation
AU2003298787A1 (en) 2002-11-27 2004-06-23 Gtc Biotherapeutics, Inc. Modified antibodies stably produced in milk and methods of producing same
EP2368578A1 (fr) 2003-01-09 2011-09-28 Macrogenics, Inc. Identification et ingénierie d'anticorps avec régions FC de variante et procédés d'utilisation associés
AR047720A1 (es) 2003-09-30 2006-02-15 Bio Sidus S A Un proceso para la obtencion de mamiferos transgenicos que produzcan proteinas exogenas en leche y los mamiferos producidos por dicho proceso
WO2006064373A2 (fr) 2004-12-16 2006-06-22 Genzyme Polyclonals S.A.S. Procedes permettant de purifier des immunoglobulines
ES2417147T3 (es) 2005-10-21 2013-08-06 Revo Biologics, Inc. Anticuerpos con actividad de citotoxicidad celular dependiente de anticuerpos mejorada, métodos para su producción y uso
FR2895263B1 (fr) 2005-12-26 2008-05-30 Lab Francais Du Fractionnement Concentre d'immunoglobines g (lg) appauvri en anticorps anti-a et anti-b, et en igg polyreactives
FR2910786B1 (fr) 2006-12-29 2017-08-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) "procede d'extraction d'une proteine presente dans du lait"
KR101834999B1 (ko) 2008-10-14 2018-03-06 제넨테크, 인크. 이뮤노글로불린 변이체 및 그의 용도
EP2233500A1 (fr) 2009-03-20 2010-09-29 LFB Biotechnologies Variantes Fc optimisées
US20120101262A1 (en) 2009-06-25 2012-04-26 Bristol-Myers Squibb Company Protein purification by caprylic acid (octanoic acid) precipitation
KR20150118103A (ko) 2013-02-06 2015-10-21 에이전시 포 사이언스, 테크놀로지 앤드 리서치 단백질 정제 방법
EP3057987B1 (fr) * 2013-10-18 2020-12-23 Novasep Process Purification de protéines
CN106164086A (zh) * 2014-02-27 2016-11-23 新加坡科技研究局 抗体纯化方法
FR3025515B1 (fr) 2014-09-05 2016-09-09 Lab Francais Du Fractionnement Procede de purification d'un anticorps monoclonal
FR3034419B1 (fr) * 2015-04-02 2017-12-15 Lab Francais Du Fractionnement Procede de purification d'une proteine recombinante therapeutique a partir d'un lait transgenique

Also Published As

Publication number Publication date
WO2019129848A1 (fr) 2019-07-04
FR3076294A1 (fr) 2019-07-05
US20210024616A1 (en) 2021-01-28
EP3732188A1 (fr) 2020-11-04

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