FR3105977B1 - Méthode de fabrication de plantes bioluminescentes - Google Patents

Méthode de fabrication de plantes bioluminescentes Download PDF

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Publication number
FR3105977B1
FR3105977B1 FR2000104A FR2000104A FR3105977B1 FR 3105977 B1 FR3105977 B1 FR 3105977B1 FR 2000104 A FR2000104 A FR 2000104A FR 2000104 A FR2000104 A FR 2000104A FR 3105977 B1 FR3105977 B1 FR 3105977B1
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France
Prior art keywords
making
plants
bioluminescent
bioluminescent plants
chloroplasts
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FR2000104A
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English (en)
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FR3105977A1 (fr
Inventor
Rose-Marie Vesin-Auclair
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Woodlight
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Woodlight
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Priority to FR2000104A priority Critical patent/FR3105977B1/fr
Priority to US17/791,253 priority patent/US20230416768A1/en
Priority to EP21700110.6A priority patent/EP4087935A1/fr
Priority to PCT/EP2021/050103 priority patent/WO2021140108A1/fr
Publication of FR3105977A1 publication Critical patent/FR3105977A1/fr
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/375Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Basidiomycetes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214Plastid transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention se rapporte à une méthode de fabrication de plantes bioluminescentes par introduction des gènes de la luciférase et ceux de la biosynthèse de luciférine dans les chloroplastes de plantes.
FR2000104A 2020-01-07 2020-01-07 Méthode de fabrication de plantes bioluminescentes Active FR3105977B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR2000104A FR3105977B1 (fr) 2020-01-07 2020-01-07 Méthode de fabrication de plantes bioluminescentes
US17/791,253 US20230416768A1 (en) 2020-01-07 2021-01-06 Method for producing bioluminescent plants
EP21700110.6A EP4087935A1 (fr) 2020-01-07 2021-01-06 Méthode de fabrication de plantes bioluminescentes
PCT/EP2021/050103 WO2021140108A1 (fr) 2020-01-07 2021-01-06 Méthode de fabrication de plantes bioluminescentes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2000104A FR3105977B1 (fr) 2020-01-07 2020-01-07 Méthode de fabrication de plantes bioluminescentes
FR2000104 2020-01-07

Publications (2)

Publication Number Publication Date
FR3105977A1 FR3105977A1 (fr) 2021-07-09
FR3105977B1 true FR3105977B1 (fr) 2022-02-11

Family

ID=70008858

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2000104A Active FR3105977B1 (fr) 2020-01-07 2020-01-07 Méthode de fabrication de plantes bioluminescentes

Country Status (4)

Country Link
US (1) US20230416768A1 (fr)
EP (1) EP4087935A1 (fr)
FR (1) FR3105977B1 (fr)
WO (1) WO2021140108A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114015703B (zh) * 2021-10-21 2023-05-26 浙江大学杭州国际科创中心 油菜BnC3H基因在提高植物生物自发光强度中的应用
CN116732084A (zh) * 2023-05-05 2023-09-12 浙江大学 真菌荧光素酶截短体在提高真菌或植物生物发光强度中的应用
CN119242698B (zh) * 2024-10-25 2026-01-06 合肥神笔生物科技有限公司 一种引入外源咖啡酸合成通路以提高植物发光强度的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
WO1997047771A1 (fr) * 1996-06-14 1997-12-18 Rutgers, The State University Of New Jersey Genes marqueurs plastidiaux selectionnables fondes sur l'edition
US6416973B1 (en) * 1997-08-01 2002-07-09 Schering Corporation Nucleic acids encoding mammalian cell membrane protein MDL-1
US6512162B2 (en) * 1998-07-10 2003-01-28 Calgene Llc Expression of eukaryotic peptides in plant plastids
US6492578B1 (en) * 1998-07-10 2002-12-10 Calgene Llc Expression of herbicide tolerance genes in plant plastids
US8147840B2 (en) * 2004-05-14 2012-04-03 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Human immunodeficiency virus (HIV) immunization strategies employing conformationally-stabilized, surface-occluded peptides comprising a gp41 2F5 epitope in association with lipid
US8865185B2 (en) * 2006-09-08 2014-10-21 The Trustees Of The University Of Pennsylvania Methods of use for HSV-1 and HSV-2 vaccines
WO2009017821A1 (fr) * 2007-08-01 2009-02-05 Bioglow Inc. Plantes bioluminescentes comprenant l'opéron lux bactérien et leurs procédés de fabrication
US20130074221A1 (en) * 2010-02-25 2013-03-21 Bioglow, L.L.C. Autoluminescent plants including the bacterial lux operon and methods of making same
RU2730038C2 (ru) * 2018-06-28 2020-08-14 Общество с ограниченной ответственностью "ПЛАНТА" Ферменты биосинтеза люциферина и их применение

Also Published As

Publication number Publication date
FR3105977A1 (fr) 2021-07-09
WO2021140108A1 (fr) 2021-07-15
US20230416768A1 (en) 2023-12-28
EP4087935A1 (fr) 2022-11-16

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