FR3131636B1 - Procédé et système pour détecter et éventuellement identifier un micro-organisme contenu dans un échantillon - Google Patents

Procédé et système pour détecter et éventuellement identifier un micro-organisme contenu dans un échantillon Download PDF

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Publication number
FR3131636B1
FR3131636B1 FR2114688A FR2114688A FR3131636B1 FR 3131636 B1 FR3131636 B1 FR 3131636B1 FR 2114688 A FR2114688 A FR 2114688A FR 2114688 A FR2114688 A FR 2114688A FR 3131636 B1 FR3131636 B1 FR 3131636B1
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France
Prior art keywords
sample
detecting
microorganism contained
measuring
derivative
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Active
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FR2114688A
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English (en)
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FR3131636A1 (fr
Inventor
Thibaut Babin
Maxime Gougis
Pascal Mailley
Pierre Marcoux
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
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Application filed by Commissariat a lEnergie Atomique CEA, Commissariat a lEnergie Atomique et aux Energies Alternatives CEA filed Critical Commissariat a lEnergie Atomique CEA
Priority to FR2114688A priority Critical patent/FR3131636B1/fr
Priority to EP22847619.8A priority patent/EP4457353A1/fr
Priority to US18/724,711 priority patent/US20250154551A1/en
Priority to PCT/FR2022/052517 priority patent/WO2023126618A1/fr
Publication of FR3131636A1 publication Critical patent/FR3131636A1/fr
Application granted granted Critical
Publication of FR3131636B1 publication Critical patent/FR3131636B1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/301—Reference electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé pour détecter et éventuellement identifier un micro-organisme contenu dans un échantillon comprenant les étapes consistant à (a) placer l’échantillon, dans un milieu de culture liquide, en présence d'une électrode de mesure et d’une électrode de référence et dans des conditions adaptées à la croissance des micro-organismes, (b) mesurer, au cours du temps, la différence de potentiel entre l’électrode de mesure et l’électrode de référence, (c) calculer la dérivée de la différence de potentiel à partir des mesures effectuées à l’étape b) moyennant quoi le profil de la dérivée du potentiel est obtenu, et (d) détecter et éventuellement identifier un micro-organisme contenu dans ledit échantillon à partir du profil obtenu à l’étape c). La présente invention concerne également un système susceptible d’être mis en œuvre lors de ce procédé.
FR2114688A 2021-12-30 2021-12-30 Procédé et système pour détecter et éventuellement identifier un micro-organisme contenu dans un échantillon Active FR3131636B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR2114688A FR3131636B1 (fr) 2021-12-30 2021-12-30 Procédé et système pour détecter et éventuellement identifier un micro-organisme contenu dans un échantillon
EP22847619.8A EP4457353A1 (fr) 2021-12-30 2022-12-29 Procédé et système pour détecter et identifier un micro-organisme contenu dans un échantillon
US18/724,711 US20250154551A1 (en) 2021-12-30 2022-12-29 Method and system for detecting and identifying a micro-organism contained in a sample
PCT/FR2022/052517 WO2023126618A1 (fr) 2021-12-30 2022-12-29 Procédé et système pour détecter et identifier un micro-organisme contenu dans un échantillon

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2114688 2021-12-30
FR2114688A FR3131636B1 (fr) 2021-12-30 2021-12-30 Procédé et système pour détecter et éventuellement identifier un micro-organisme contenu dans un échantillon

Publications (2)

Publication Number Publication Date
FR3131636A1 FR3131636A1 (fr) 2023-07-07
FR3131636B1 true FR3131636B1 (fr) 2025-01-03

Family

ID=81449001

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2114688A Active FR3131636B1 (fr) 2021-12-30 2021-12-30 Procédé et système pour détecter et éventuellement identifier un micro-organisme contenu dans un échantillon

Country Status (4)

Country Link
US (1) US20250154551A1 (fr)
EP (1) EP4457353A1 (fr)
FR (1) FR3131636B1 (fr)
WO (1) WO2023126618A1 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2335602A1 (fr) * 1975-12-16 1977-07-15 Univ Virginia Methode de detection et d'enumeration microbienne et appareil mis en oeuvre
JPS5663249A (en) * 1979-10-27 1981-05-29 Kyoto Daiichi Kagaku:Kk Measurement of multiplication of bacteria
US4528270A (en) * 1982-11-02 1985-07-09 Kabushiki Kaisya Advance Kaihatsu Kenkyujo Electrochemical method for detection and classification of microbial cell
FR3100034B1 (fr) 2019-08-22 2024-03-15 Commissariat Energie Atomique Conteneur instrumenté et système incluant un caisson et ledit conteneur pour la détection de présence de micro-organismes dans un échantillon liquide

Also Published As

Publication number Publication date
FR3131636A1 (fr) 2023-07-07
US20250154551A1 (en) 2025-05-15
EP4457353A1 (fr) 2024-11-06
WO2023126618A1 (fr) 2023-07-06

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