FR3134822B1 - milieu réactionnel et méthode associée pour la détection, d’un micro-organisme cible. - Google Patents

milieu réactionnel et méthode associée pour la détection, d’un micro-organisme cible.

Info

Publication number
FR3134822B1
FR3134822B1 FR2203888A FR2203888A FR3134822B1 FR 3134822 B1 FR3134822 B1 FR 3134822B1 FR 2203888 A FR2203888 A FR 2203888A FR 2203888 A FR2203888 A FR 2203888A FR 3134822 B1 FR3134822 B1 FR 3134822B1
Authority
FR
France
Prior art keywords
detection
reaction medium
target microorganism
associated method
microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR2203888A
Other languages
English (en)
Other versions
FR3134822A1 (fr
Inventor
Thomas Junillon
Benoît Mallen
Patrice Chablain
David Mosticone
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR2203888A priority Critical patent/FR3134822B1/fr
Application filed by Biomerieux SA filed Critical Biomerieux SA
Priority to AU2023262222A priority patent/AU2023262222A1/en
Priority to PCT/FR2023/000050 priority patent/WO2023209287A1/fr
Priority to JP2024563221A priority patent/JP2025513618A/ja
Priority to EP23724344.9A priority patent/EP4514991A1/fr
Priority to CA3250241A priority patent/CA3250241A1/fr
Priority to CN202380035526.0A priority patent/CN119137281A/zh
Priority to US18/858,962 priority patent/US20250277250A1/en
Publication of FR3134822A1 publication Critical patent/FR3134822A1/fr
Application granted granted Critical
Publication of FR3134822B1 publication Critical patent/FR3134822B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G01N33/5304—Reaction vessels, e.g. agglutination plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/50—Lipopolysaccharides; LPS

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Nanotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L’invention concerne un milieu réactionnel gélifié pour la détection, l’identification, le dénombrement et/ou l’isolement d’au moins un micro-organisme cible dans un échantillon susceptible de le contenir comprenant au moins un partenaire de liaison spécifique d’un composant d’un micro-organisme cible ou d’un composant issu dudit micro-organisme, couplé à au moins une nanoparticule pour former au moins un conjugué agglutinant.
FR2203888A 2022-04-26 2022-04-26 milieu réactionnel et méthode associée pour la détection, d’un micro-organisme cible. Active FR3134822B1 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FR2203888A FR3134822B1 (fr) 2022-04-26 2022-04-26 milieu réactionnel et méthode associée pour la détection, d’un micro-organisme cible.
PCT/FR2023/000050 WO2023209287A1 (fr) 2022-04-26 2023-04-25 Milieu réactionnel et méthode associée pour la détection, d'un micro-organisme cible
JP2024563221A JP2025513618A (ja) 2022-04-26 2023-04-25 標的微生物を検出するための反応媒体、及び関連する方法
EP23724344.9A EP4514991A1 (fr) 2022-04-26 2023-04-25 Milieu réactionnel et méthode associée pour la détection, d'un micro-organisme cible
AU2023262222A AU2023262222A1 (en) 2022-04-26 2023-04-25 Reaction medium and associated method for detection of a target microorganism
CA3250241A CA3250241A1 (fr) 2022-04-26 2023-04-25 Milieu réactionnel et méthode associée pour la détection, d’un micro-organisme cible
CN202380035526.0A CN119137281A (zh) 2022-04-26 2023-04-25 用于检测目标微生物的反应培养基和相关方法
US18/858,962 US20250277250A1 (en) 2022-04-26 2023-04-25 Reaction medium and associated method for detection of a target microorganism

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2203888A FR3134822B1 (fr) 2022-04-26 2022-04-26 milieu réactionnel et méthode associée pour la détection, d’un micro-organisme cible.
FR2203888 2022-04-26

Publications (2)

Publication Number Publication Date
FR3134822A1 FR3134822A1 (fr) 2023-10-27
FR3134822B1 true FR3134822B1 (fr) 2026-01-02

Family

ID=83439203

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2203888A Active FR3134822B1 (fr) 2022-04-26 2022-04-26 milieu réactionnel et méthode associée pour la détection, d’un micro-organisme cible.

Country Status (8)

Country Link
US (1) US20250277250A1 (fr)
EP (1) EP4514991A1 (fr)
JP (1) JP2025513618A (fr)
CN (1) CN119137281A (fr)
AU (1) AU2023262222A1 (fr)
CA (1) CA3250241A1 (fr)
FR (1) FR3134822B1 (fr)
WO (1) WO2023209287A1 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3773633B2 (ja) * 1997-10-31 2006-05-10 株式会社三菱化学ヤトロン 大腸菌ｏ１５７の分析方法及び分析用試薬
FR2928656B1 (fr) * 2008-03-14 2011-08-26 Biomerieux Sa Procede de detection en temps reel de microorganismes dans un milieu de culture liquide par agglutination.
FR2962445B1 (fr) * 2010-07-08 2013-06-28 Biomerieux Sa Procede de detection et d'identification directe d'un microorganisme dans un echantillon biologique dilue dans un bouillon d'enrichissement
KR101444424B1 (ko) * 2011-10-14 2014-09-29 한국생명공학연구원 두 종 입자를 이용한 다기능 생체물질 접합체의 제조방법 및 이로부터 제조된 다기능 생체물질 접합체

Also Published As

Publication number Publication date
EP4514991A1 (fr) 2025-03-05
US20250277250A1 (en) 2025-09-04
WO2023209287A1 (fr) 2023-11-02
FR3134822A1 (fr) 2023-10-27
CA3250241A1 (fr) 2023-11-02
JP2025513618A (ja) 2025-04-24
AU2023262222A1 (en) 2024-12-12
CN119137281A (zh) 2024-12-13

Similar Documents

Publication Publication Date Title
Lin et al. Tracing tumor‐derived exosomal PD‐L1 by dual‐aptamer activated proximity‐induced droplet digital PCR
Lehrer et al. Functional characterization and membrane topology of Escherichia coli WecA, a sugar-phosphate transferase initiating the biosynthesis of enterobacterial common antigen and O-antigen lipopolysaccharide
Cabantous et al. Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein
US20170115299A1 (en) High throughput detection of fusion proteins
CN112105743A (zh) 复用型催化报告分子沉积
Zhang et al. Development of a highly sensitive lateral flow immunoassay based on receptor-antibody-amorphous carbon nanoparticles to detect 22 β-lactams in milk
CN105431575A (zh) 磁性免疫数字pcr试验
Peng et al. Development of a direct ELISA based on carboxy-terminal of penicillin-binding protein BlaR for the detection of β-lactam antibiotics in foods
RS58212B1 (sr) Testovi za otkrivanje prisustva ili količine anti-lek antitela
US20070281367A1 (en) Methods, Compositions, and Kits for Quantitating Antibodies
Gehring et al. Antibody Microarray Detection of Escherichia c oli O157: H7: Quantification, Assay Limitations, and Capture Efficiency
Liu et al. Rapid and multi-target genotyping of Helicobacter pylori with digital microfluidics
US20240044882A1 (en) Tethered detection assays
US11300567B2 (en) Methods and compositions for protein purification and enzyme reaction
Santos et al. LUMINEX®: a new technology for the simultaneous identification of five Entamoeba spp. commonly found in human stools
US8663942B2 (en) Means and methods for the determination of the amount of neurotoxin polypeptide and of its catalytic and proteolytic activities
FR3134822B1 (fr) milieu réactionnel et méthode associée pour la détection, d’un micro-organisme cible.
He et al. Immuno-PCR assay for sensitive detection of proteins in real time
Kinoshita‐Kikuta et al. Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos‐tag Magenta/Cyan fluorescent dyes
Heuer et al. Paving the way to overcome antifungal drug resistance: current practices and novel developments for rapid and reliable antifungal susceptibility testing
CN106233144A (zh) 使用热移位测定用于鉴定指示药物应答减少的生物标记物的方法
WO2021211969A3 (fr) Immunoessai d'extinction de fluorescence
US20180095076A1 (en) Linked Peptide Fluorogenic Biosensors
Yuan et al. Digital droplet RT‐LAMP increases speed of SARS‐CoV‐2 viral RNA detection
Grate et al. Renewable surface fluorescence sandwich immunoassay biosensor for rapid sensitive botulinum toxin detection in an automated fluidic format

Legal Events

Date Code Title Description
PLFP Fee payment

Year of fee payment: 2

PLSC Publication of the preliminary search report

Effective date: 20231027

PLFP Fee payment

Year of fee payment: 3

PLFP Fee payment

Year of fee payment: 4