FR3136479B1 - Dispositif d’isolement de cellules de sperme et procédé de sélection de cellules de sperme de haute qualité - Google Patents

Dispositif d’isolement de cellules de sperme et procédé de sélection de cellules de sperme de haute qualité

Info

Publication number
FR3136479B1
FR3136479B1 FR2205675A FR2205675A FR3136479B1 FR 3136479 B1 FR3136479 B1 FR 3136479B1 FR 2205675 A FR2205675 A FR 2205675A FR 2205675 A FR2205675 A FR 2205675A FR 3136479 B1 FR3136479 B1 FR 3136479B1
Authority
FR
France
Prior art keywords
area
outlet
inlet
sperm
sperm cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR2205675A
Other languages
English (en)
Other versions
FR3136479A1 (fr
Inventor
Shiva Kant Shukla
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beez Biotech
Original Assignee
Beez Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beez Biotech filed Critical Beez Biotech
Priority to FR2205675A priority Critical patent/FR3136479B1/fr
Priority to EP23732542.8A priority patent/EP4536800A1/fr
Priority to CN202380058914.0A priority patent/CN119630781A/zh
Priority to US18/874,183 priority patent/US20250367664A1/en
Priority to PCT/EP2023/065838 priority patent/WO2023242209A1/fr
Publication of FR3136479A1 publication Critical patent/FR3136479A1/fr
Application granted granted Critical
Publication of FR3136479B1 publication Critical patent/FR3136479B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Reproductive Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Public Health (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un dispositif de sélection de cellules de sperme ayant une plaque de support comprenant une entrée pour recevoir un échantillon de cellules de sperme, une sortie pour collecter au moins une partie des cellules de sperme comprises dans ledit échantillon, et un système microfluidique entre lesdites entrée et sortie, dans lequel le système microfluidique comprend une cuvette agencée pour recevoir un milieu et ayant une zone d'entrée et une zone de sortie respectivement à proximité de l'entrée et de la sortie, et dans lequel l'entrée et la sortie sont agencées pour fournir une différence de pression hydrostatique entre ladite zone d'entrée et ladite zone de sortie lorsqu'un milieu est ajouté à la cuvette par l'intermédiaire de la sortie, de manière à provoquer un courant dudit milieu depuis la zone de sortie en direction de la zone d'entrée pour initier la migration de cellules de sperme des cellules de sperme de l'échantillon de cellules de sperme depuis la zone d'entrée en direction de la zone de sortie, et dans lequel la zone d'entrée et la zone de sortie sont en communication fluidique l'une avec l'autre par l’intermédiaire d’un canal principal agencé dans la cuvette, le canal ayant une zone de largeur réduite agencée entre la zone d'entrée et la zone de sortie de manière à former une zone de rhéotaxie, moyennant quoi ledit échantillon de cellules de sperme subit une sélection en passant à travers ladite zone de rhéotaxie durant ladite migration de cellules de sperme permettant ainsi de collecter un échantillon de cellules de sperme de qualité de la zone de sortie au niveau de la sortie. Figure pour l’abrégé : Fig. 1
FR2205675A 2022-06-13 2022-06-13 Dispositif d’isolement de cellules de sperme et procédé de sélection de cellules de sperme de haute qualité Active FR3136479B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR2205675A FR3136479B1 (fr) 2022-06-13 2022-06-13 Dispositif d’isolement de cellules de sperme et procédé de sélection de cellules de sperme de haute qualité
EP23732542.8A EP4536800A1 (fr) 2022-06-13 2023-06-13 Dispositif pour i'isolement de spermatozoïdes et procédé pour la sélection de spermatozoïdes de haute qualité
CN202380058914.0A CN119630781A (zh) 2022-06-13 2023-06-13 精子细胞分离装置及优质精子细胞的选择方法
US18/874,183 US20250367664A1 (en) 2022-06-13 2023-06-13 Device for sperm cell isolation and method for selection of high quality sperm cells
PCT/EP2023/065838 WO2023242209A1 (fr) 2022-06-13 2023-06-13 Dispositif pour i'isolement de spermatozoïdes et procédé pour la sélection de spermatozoïdes de haute qualité

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2205675 2022-06-13
FR2205675A FR3136479B1 (fr) 2022-06-13 2022-06-13 Dispositif d’isolement de cellules de sperme et procédé de sélection de cellules de sperme de haute qualité

Publications (2)

Publication Number Publication Date
FR3136479A1 FR3136479A1 (fr) 2023-12-15
FR3136479B1 true FR3136479B1 (fr) 2025-08-29

Family

ID=84359861

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2205675A Active FR3136479B1 (fr) 2022-06-13 2022-06-13 Dispositif d’isolement de cellules de sperme et procédé de sélection de cellules de sperme de haute qualité

Country Status (5)

Country Link
US (1) US20250367664A1 (fr)
EP (1) EP4536800A1 (fr)
CN (1) CN119630781A (fr)
FR (1) FR3136479B1 (fr)
WO (1) WO2023242209A1 (fr)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2421360B1 (fr) 2009-04-22 2014-05-07 Lotus Bio (Nymphaea) Ltd. Systeme de separation du sperme
WO2012126478A1 (fr) * 2011-03-21 2012-09-27 Motilitycount Aps Dispositif d'analyse de la motilité cellulaire
TR201819543T4 (tr) 2011-05-20 2019-01-21 Brigham & Womens Hospital Inc Hareketli hücrelerin analizi ve ayıklanması.
US10450545B2 (en) * 2011-09-14 2019-10-22 National Tsing Hua University Microfluidic chips for acquiring sperms with high motility, productions and applications thereof
WO2020041303A1 (fr) 2018-08-21 2020-02-27 Florida Atlantic University Board Of Trustees Systèmes et procédés de sélection de sperme

Also Published As

Publication number Publication date
US20250367664A1 (en) 2025-12-04
FR3136479A1 (fr) 2023-12-15
EP4536800A1 (fr) 2025-04-16
WO2023242209A1 (fr) 2023-12-21
CN119630781A (zh) 2025-03-14

Similar Documents

Publication Publication Date Title
FR3136479B1 (fr) Dispositif d’isolement de cellules de sperme et procédé de sélection de cellules de sperme de haute qualité
CN111549893B (zh) 一种自动排水截流井
FR2593921A1 (fr) Appareil automatique portable pour l'analyse thermodynamique de fluides souterrains
CN103994902A (zh) 一种近岸海底抽吸式采泥器
FR3086388B1 (fr) Moyen de mesure de fluide presentant un boitier de fluide, et procede de fabrication de boitier de fluide
CN111665343A (zh) 坡沟系统土壤侵蚀试验装置及试验方法
CN114561916A (zh) 一种水利灌溉用闸门传送装置
CN218212175U (zh) 一种流量测量装置
CN115711613A (zh) 一种水文测验用泥沙流量监测装置
CN205979152U (zh) 集成式疏水装置
CN204563948U (zh) 一种膜片测试单元
CN120036838A (zh) 一种检验科用尿液取样装置
JPH06331056A (ja) 可変定流量弁
CN113049522B (zh) 能够消除气泡的近红外分析装置
CN109724835B (zh) 含天然气水合物的沉积物孔隙水现场快速采集装置及方法
CN209727715U (zh) 一种耦合作用下微尺度流动可视化测试装置
CN107511192B (zh) 一种气液压力平衡调节器及包括该调节器的排水集气装置
CA1252740A (fr) Procede et dispositif permettant de realiser un ensemble de fonctions hydrodynamiques dans un ecoulement compose d'au moins deux phases
RU80240U1 (ru) Проточная спектрофотометрическая кювета
CN221945663U (zh) 一种水质采样器
FR2645705A1 (fr) Appareil d'echantillonnage d'un liquide et machine automatique de traite
CN215414555U (zh) 一种连续取样装置
CN114646504A (zh) 一种水文水资源取样装置
CN207351727U (zh) 一种土壤微生物样品采集工具
NL2038017B1 (en) In-situ sampling device for heavy metal released from river and lake sediment

Legal Events

Date Code Title Description
PLFP Fee payment

Year of fee payment: 2

PLSC Publication of the preliminary search report

Effective date: 20231215

PLFP Fee payment

Year of fee payment: 3

PLFP Fee payment

Year of fee payment: 4