FR3138211B1 - Dispositif pour le comptage et la différenciation de particules d’un flux d’échantillon - Google Patents

Dispositif pour le comptage et la différenciation de particules d’un flux d’échantillon

Info

Publication number
FR3138211B1
FR3138211B1 FR2207619A FR2207619A FR3138211B1 FR 3138211 B1 FR3138211 B1 FR 3138211B1 FR 2207619 A FR2207619 A FR 2207619A FR 2207619 A FR2207619 A FR 2207619A FR 3138211 B1 FR3138211 B1 FR 3138211B1
Authority
FR
France
Prior art keywords
sample stream
sleeved
particles
counting
stream
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR2207619A
Other languages
English (en)
Other versions
FR3138211A1 (fr
Inventor
Patrick Brunel
Didier Cremien
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Horiba ABX SAS
Original Assignee
Horiba ABX SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Horiba ABX SAS filed Critical Horiba ABX SAS
Priority to FR2207619A priority Critical patent/FR3138211B1/fr
Priority to EP23755135.3A priority patent/EP4562399A1/fr
Priority to PCT/FR2023/051146 priority patent/WO2024023430A1/fr
Publication of FR3138211A1 publication Critical patent/FR3138211A1/fr
Application granted granted Critical
Publication of FR3138211B1 publication Critical patent/FR3138211B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1019—Associating Coulter-counter and optical flow cytometer [OFC]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1024—Counting particles by non-optical means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L’invention concerne un dispositif (1) pour le comptage et la différenciation de particules comprenant : - une embase chauffante (3) au sein de laquelle sont ménagés un conduit d’injection (9) d’un flux d’échantillon et un conduit d’injection (11) d’un flux de manchonnage, et - une cellule de mesure (5) comprenant une cuve optique (17) agencée pour être traversée par un flux d’échantillon manchonné, une source lumineuse (33) agencée pour éclairer le flux d’échantillon manchonné, un détecteur (41) agencé pour recevoir un signal de fluorescence émis par une particule après absorption de la lumière, et un conduit d’évacuation (23) du flux d’échantillon manchonné. Le détecteur (41) est agencé pour mesurer une fluorescence afin de différencier les particules. L’embase chauffante (3) et le conduit d’évacuation (23) forment des électrodes de mesure agencées pour mesurer une résistivité afin de compter les particules. [Fig. 1]
FR2207619A 2022-07-25 2022-07-25 Dispositif pour le comptage et la différenciation de particules d’un flux d’échantillon Active FR3138211B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR2207619A FR3138211B1 (fr) 2022-07-25 2022-07-25 Dispositif pour le comptage et la différenciation de particules d’un flux d’échantillon
EP23755135.3A EP4562399A1 (fr) 2022-07-25 2023-07-24 Dispositif pour le comptage et la différenciation de particules d?un flux d?échantillon
PCT/FR2023/051146 WO2024023430A1 (fr) 2022-07-25 2023-07-24 Dispositif pour le comptage et la différenciation de particules d'un flux d'échantillon

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2207619A FR3138211B1 (fr) 2022-07-25 2022-07-25 Dispositif pour le comptage et la différenciation de particules d’un flux d’échantillon
FR2207619 2022-07-25

Publications (2)

Publication Number Publication Date
FR3138211A1 FR3138211A1 (fr) 2024-01-26
FR3138211B1 true FR3138211B1 (fr) 2026-03-13

Family

ID=83506128

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2207619A Active FR3138211B1 (fr) 2022-07-25 2022-07-25 Dispositif pour le comptage et la différenciation de particules d’un flux d’échantillon

Country Status (3)

Country Link
EP (1) EP4562399A1 (fr)
FR (1) FR3138211B1 (fr)
WO (1) WO2024023430A1 (fr)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59184841A (ja) * 1983-04-05 1984-10-20 ベクトン・デイツキンソン・アンド・カンパニ− サンプル中の白血球のサブクラスを識別する方法および装置
FR2653885B1 (fr) 1989-10-27 1994-01-14 Abx Appareil pour le comptage et la determination d'au moins une sous-population leucocytaire.
KR102095617B1 (ko) * 2013-03-15 2020-03-31 아이리스 인터내셔널 인크. 혈액 샘플에서의 입자 분석을 위한 오토포커스 시스템 및 방법
US9857361B2 (en) * 2013-03-15 2018-01-02 Iris International, Inc. Flowcell, sheath fluid, and autofocus systems and methods for particle analysis in urine samples
FR3106408B1 (fr) 2020-01-17 2024-06-07 Horiba Abx Sas Dispositif électro-optique de mesures en flux

Also Published As

Publication number Publication date
EP4562399A1 (fr) 2025-06-04
WO2024023430A1 (fr) 2024-02-01
FR3138211A1 (fr) 2024-01-26

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