FR3146214B1 - Lame optique destinée à la microscopie par réflexion totale interne, dispositif de microscopie par réflexion totale interne comportant une telle lame et procédé de fabrication d’une telle lame - Google Patents
Lame optique destinée à la microscopie par réflexion totale interne, dispositif de microscopie par réflexion totale interne comportant une telle lame et procédé de fabrication d’une telle lame Download PDFInfo
- Publication number
- FR3146214B1 FR3146214B1 FR2301673A FR2301673A FR3146214B1 FR 3146214 B1 FR3146214 B1 FR 3146214B1 FR 2301673 A FR2301673 A FR 2301673A FR 2301673 A FR2301673 A FR 2301673A FR 3146214 B1 FR3146214 B1 FR 3146214B1
- Authority
- FR
- France
- Prior art keywords
- slide
- internal reflection
- total internal
- reflection microscopy
- called
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
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Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B1/00—Optical elements characterised by the material of which they are made; Optical coatings for optical elements
- G02B1/002—Optical elements characterised by the material of which they are made; Optical coatings for optical elements made of materials engineered to provide properties not available in nature, e.g. metamaterials
- G02B1/005—Optical elements characterised by the material of which they are made; Optical coatings for optical elements made of materials engineered to provide properties not available in nature, e.g. metamaterials made of photonic crystals or photonic band gap materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/56—Optics using evanescent waves, i.e. inhomogeneous waves
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/20—Filters
- G02B5/26—Reflecting filters
- G02B5/265—Reflecting filters involving total internal reflection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microscoopes, Condenser (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Lame optique (LO) destinée à recevoir un échantillon biologique (E) dans un dispositif de microscopie de fluorescence par réflexion totale interne, la lame optique comprenant : une couche dite substrat (SB) transparente dans une région spectrale prédéterminée un empilement dit optimisé disposé au dessus du substrat (SB) et comprenant un nombre de couches minces diélectriques successives et alternées d’un premier matériau diélectrique et d’un deuxième matériau diélectrique, chacune des couches présentant une épaisseur dite optimisée respective, les épaisseurs optimisées et le nombre de couches minces diélectriques étant adaptés de manière à ce que ladite lame optique présente au moins :une première absorption résonnante à une longueur d’onde dite d’illumination et à un premier angle d’incidence en régime de réflexion totale interne, et une deuxième absorption résonnante à la longueur d’onde d’illumination et à un deuxième angle d’incidence en régime de réflexion totale interne, avec et ou . Fig. 3
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR2301673A FR3146214B1 (fr) | 2023-02-23 | 2023-02-23 | Lame optique destinée à la microscopie par réflexion totale interne, dispositif de microscopie par réflexion totale interne comportant une telle lame et procédé de fabrication d’une telle lame |
| PCT/EP2024/054550 WO2024175729A1 (fr) | 2023-02-23 | 2024-02-22 | Lame optique pour un dispositif optique par réflexion totale interne, son procédé de fabrication et dispositif optique comportant une telle lame |
| EP24705703.7A EP4669990A1 (fr) | 2023-02-23 | 2024-02-22 | Lame optique pour un dispositif optique par réflexion totale interne, son procédé de fabrication et dispositif optique comportant une telle lame |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR2301673 | 2023-02-23 | ||
| FR2301673A FR3146214B1 (fr) | 2023-02-23 | 2023-02-23 | Lame optique destinée à la microscopie par réflexion totale interne, dispositif de microscopie par réflexion totale interne comportant une telle lame et procédé de fabrication d’une telle lame |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR3146214A1 FR3146214A1 (fr) | 2024-08-30 |
| FR3146214B1 true FR3146214B1 (fr) | 2025-03-14 |
Family
ID=86331845
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR2301673A Active FR3146214B1 (fr) | 2023-02-23 | 2023-02-23 | Lame optique destinée à la microscopie par réflexion totale interne, dispositif de microscopie par réflexion totale interne comportant une telle lame et procédé de fabrication d’une telle lame |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP4669990A1 (fr) |
| FR (1) | FR3146214B1 (fr) |
| WO (1) | WO2024175729A1 (fr) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2108879A1 (fr) | 1970-10-12 | 1972-05-26 | Goiffon Leon Ets | |
| US9733465B2 (en) | 2015-02-12 | 2017-08-15 | The Penn State Research Foundation | Waveguides for enhanced total internal reflection fluorescence microscopy |
| DE102019102604A1 (de) * | 2019-02-01 | 2020-08-06 | Carl Zeiss Jena Gmbh | Funktionalisierter Wellenleiter für ein Detektorsystem |
-
2023
- 2023-02-23 FR FR2301673A patent/FR3146214B1/fr active Active
-
2024
- 2024-02-22 WO PCT/EP2024/054550 patent/WO2024175729A1/fr not_active Ceased
- 2024-02-22 EP EP24705703.7A patent/EP4669990A1/fr active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4669990A1 (fr) | 2025-12-31 |
| WO2024175729A1 (fr) | 2024-08-29 |
| FR3146214A1 (fr) | 2024-08-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PLFP | Fee payment |
Year of fee payment: 2 |
|
| PLSC | Publication of the preliminary search report |
Effective date: 20240830 |
|
| PLFP | Fee payment |
Year of fee payment: 3 |
|
| PLFP | Fee payment |
Year of fee payment: 4 |