GB1237268A - Proteinaceous reagent and process for producing same - Google Patents
Proteinaceous reagent and process for producing sameInfo
- Publication number
- GB1237268A GB1237268A GB48249/68A GB4824968A GB1237268A GB 1237268 A GB1237268 A GB 1237268A GB 48249/68 A GB48249/68 A GB 48249/68A GB 4824968 A GB4824968 A GB 4824968A GB 1237268 A GB1237268 A GB 1237268A
- Authority
- GB
- United Kingdom
- Prior art keywords
- reagent
- compound
- proteinaceous
- protein
- exemplified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 8
- 239000000463 material Substances 0.000 abstract 3
- 239000002245 particle Substances 0.000 abstract 3
- SMNDYUVBFMFKNZ-UHFFFAOYSA-N 2-furoic acid Chemical compound OC(=O)C1=CC=CO1 SMNDYUVBFMFKNZ-UHFFFAOYSA-N 0.000 abstract 2
- MWOOKDULMBMMPN-UHFFFAOYSA-N 3-(2-ethyl-1,2-oxazol-2-ium-5-yl)benzenesulfonate Chemical compound O1[N+](CC)=CC=C1C1=CC=CC(S([O-])(=O)=O)=C1 MWOOKDULMBMMPN-UHFFFAOYSA-N 0.000 abstract 2
- 239000000427 antigen Substances 0.000 abstract 2
- 102000036639 antigens Human genes 0.000 abstract 2
- 108091007433 antigens Proteins 0.000 abstract 2
- DMBHHRLKUKUOEG-UHFFFAOYSA-N diphenylamine Chemical compound C=1C=CC=CC=1NC1=CC=CC=C1 DMBHHRLKUKUOEG-UHFFFAOYSA-N 0.000 abstract 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- LLPCTHRRXMFBHO-UHFFFAOYSA-N 1h-pyrrole-2-carbonyl azide Chemical compound [N-]=[N+]=NC(=O)C1=CC=CN1 LLPCTHRRXMFBHO-UHFFFAOYSA-N 0.000 abstract 1
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 abstract 1
- 241000270728 Alligator Species 0.000 abstract 1
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 241000194103 Bacillus pumilus Species 0.000 abstract 1
- 241000283690 Bos taurus Species 0.000 abstract 1
- 241000589567 Brucella abortus Species 0.000 abstract 1
- 241000283707 Capra Species 0.000 abstract 1
- 241000700199 Cavia porcellus Species 0.000 abstract 1
- 241000289427 Didelphidae Species 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 241000287828 Gallus gallus Species 0.000 abstract 1
- 108010010803 Gelatin Proteins 0.000 abstract 1
- 241000282414 Homo sapiens Species 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 241000186660 Lactobacillus Species 0.000 abstract 1
- 102000036675 Myoglobin Human genes 0.000 abstract 1
- 108010062374 Myoglobin Proteins 0.000 abstract 1
- 241000283973 Oryctolagus cuniculus Species 0.000 abstract 1
- 241001494479 Pecora Species 0.000 abstract 1
- 239000004793 Polystyrene Substances 0.000 abstract 1
- 241000589538 Pseudomonas fragi Species 0.000 abstract 1
- 241000700159 Rattus Species 0.000 abstract 1
- 241000270666 Testudines Species 0.000 abstract 1
- 239000012190 activator Substances 0.000 abstract 1
- 150000001491 aromatic compounds Chemical class 0.000 abstract 1
- 125000003118 aryl group Chemical group 0.000 abstract 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 abstract 1
- 229940056450 brucella abortus Drugs 0.000 abstract 1
- 239000002775 capsule Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000008878 coupling Effects 0.000 abstract 1
- 238000010168 coupling process Methods 0.000 abstract 1
- 238000005859 coupling reaction Methods 0.000 abstract 1
- 125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 abstract 1
- 238000010790 dilution Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 abstract 1
- 238000007336 electrophilic substitution reaction Methods 0.000 abstract 1
- 210000003743 erythrocyte Anatomy 0.000 abstract 1
- 108010074605 gamma-Globulins Proteins 0.000 abstract 1
- 229920000159 gelatin Polymers 0.000 abstract 1
- 239000008273 gelatin Substances 0.000 abstract 1
- 235000019322 gelatine Nutrition 0.000 abstract 1
- 235000011852 gelatine desserts Nutrition 0.000 abstract 1
- -1 haemoglobin Proteins 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- 229940039696 lactobacillus Drugs 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 229920002223 polystyrene Polymers 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- CTBMQGOJWHCMGI-UHFFFAOYSA-N thiophene-2-carbonyl azide Chemical compound [N-]=[N+]=NC(=O)C1=CC=CS1 CTBMQGOJWHCMGI-UHFFFAOYSA-N 0.000 abstract 1
- VNNLHYZDXIBHKZ-UHFFFAOYSA-N thiophene-2-sulfonyl chloride Chemical compound ClS(=O)(=O)C1=CC=CS1 VNNLHYZDXIBHKZ-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
1,237,268. Proteinaceous reagent. MILES LABORATORIES Inc. 11 Oct., 1968 [1 Nov., 1967], No. 48249/68. Heading B1X. [Also in Divisions C3 and C6] A proteinaceous reagent is prepared by a process comprising (a) treating an antigen, antibody or enzyme with either (i) a bis-diazotised aromatic compound capable of coupling with protein, or (ii) a linking compound (defined below), (b) treating protein-surfaced insoluble particles with whichever of compounds (i) or (ii) is not used in step (a), and (c) reacting the two treated materials together so that the unreacted diazo groups of compound (i) couple with the linking compound (ii) to form the proteinaceous reagent. Compound (i) may be bis-diazotised benzidine, dianisidine, or 4, 4', diphenylamine. The linking compound (ii) is a compound reactive with proteinaceous material and containing an aromatic radical which is more susceptible to electrophilic substitution than any aromatic material occurring in protein. Compounds (ii) exemplified are pyrrole-2-carboxylic acid azide, thiophene-2-carboxylic acid azide, 2-furoic acid with N-ethyl-5-phenylisoxazolium-3'- sulphonate (Woodward's reagent K) as activator, 2-thiophenesulphonyl chloride; others are mentioned. The protein-surfaced insoluble particles may be red blood cells (opossum, rat, rabbit, guinea pig, human, goat, sheep, chicken, alligator, turtle), microbial cells (preferably Brucella abortus, Escherichia coli (exemplified), Bacillus substilis, Bacillus pumilis, Lactobacillus kichmannii, Pseudomonas fragi, or Saccharonyees cerevisiae), or 0.1 to 10 microns size particles of for example polystyrene coated with gelatin. Antigens &c exemplified are myoglobin, HCG, haemoglobin, BSA, bovine gamma globulin, insulin; others are mentioned. Unit pack capsules may be prepared each carrying in different areas the lyophilized residues of a drop of a suspension of the reagent and of a dilution of the appropriate antiserum.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67962867A | 1967-11-01 | 1967-11-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1237268A true GB1237268A (en) | 1971-06-30 |
Family
ID=24727678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB48249/68A Expired GB1237268A (en) | 1967-11-01 | 1968-10-11 | Proteinaceous reagent and process for producing same |
Country Status (6)
| Country | Link |
|---|---|
| DE (1) | DE1806418A1 (en) |
| ES (1) | ES359805A1 (en) |
| FR (1) | FR1590466A (en) |
| GB (1) | GB1237268A (en) |
| IL (1) | IL30997A (en) |
| NL (1) | NL6815548A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0023431A1 (en) * | 1979-07-30 | 1981-02-04 | Brocades (Great Britain) Limited | Processes for the production of carrier particles from yeast cells and for producing diagnostic reagents therefrom; carrier particles and diagnostic reagent produced thereby and test kit containing such reagents |
| WO2006063592A1 (en) * | 2004-12-14 | 2006-06-22 | Alk Abelló A/S | Pharmaceutical composition comprising a bacterial cell displaying a heterologous proteinaceous compound |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4166767A (en) * | 1976-03-25 | 1979-09-04 | Dainippon Pharmaceutical Co., Ltd. | Insolubilized antibody |
| CH628738A5 (en) * | 1977-08-03 | 1982-03-15 | Hoffmann La Roche | IMMUNOLOGICAL DIAGNOSTIC REAGENT. |
| EP0015473A1 (en) * | 1979-02-28 | 1980-09-17 | F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft | Process for immobilizing cells |
| US4452886A (en) * | 1981-09-21 | 1984-06-05 | Henry Robert P | Novel photon absorbing or emitting polymers and their use as reagents in immunoassay systems |
-
1968
- 1968-10-11 GB GB48249/68A patent/GB1237268A/en not_active Expired
- 1968-10-31 FR FR1590466D patent/FR1590466A/fr not_active Expired
- 1968-10-31 ES ES359805A patent/ES359805A1/en not_active Expired
- 1968-10-31 NL NL6815548A patent/NL6815548A/xx unknown
- 1968-10-31 DE DE19681806418 patent/DE1806418A1/en active Pending
- 1968-11-01 IL IL30997A patent/IL30997A/en unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0023431A1 (en) * | 1979-07-30 | 1981-02-04 | Brocades (Great Britain) Limited | Processes for the production of carrier particles from yeast cells and for producing diagnostic reagents therefrom; carrier particles and diagnostic reagent produced thereby and test kit containing such reagents |
| WO2006063592A1 (en) * | 2004-12-14 | 2006-06-22 | Alk Abelló A/S | Pharmaceutical composition comprising a bacterial cell displaying a heterologous proteinaceous compound |
Also Published As
| Publication number | Publication date |
|---|---|
| DE1806418A1 (en) | 1969-06-26 |
| ES359805A1 (en) | 1970-06-16 |
| IL30997A (en) | 1973-03-30 |
| NL6815548A (en) | 1969-05-05 |
| IL30997A0 (en) | 1969-01-29 |
| FR1590466A (en) | 1970-04-13 |
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