GEP20033036B - Method and Kit for Extracting Prion Protein - Google Patents

Method and Kit for Extracting Prion Protein

Info

Publication number
GEP20033036B
GEP20033036B GEAP20006037A GEAP2000006037A GEP20033036B GE P20033036 B GEP20033036 B GE P20033036B GE AP20006037 A GEAP20006037 A GE AP20006037A GE AP2000006037 A GEAP2000006037 A GE AP2000006037A GE P20033036 B GEP20033036 B GE P20033036B
Authority
GE
Georgia
Prior art keywords
prion protein
abnormal
protein
hexafluoro
propanol
Prior art date
Application number
GEAP20006037A
Inventor
Andrew J Alpert
Mary Jo Schmerr
Original Assignee
Andrew J Alpert
Us Secretary Of Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Andrew J Alpert, Us Secretary Of Agriculture filed Critical Andrew J Alpert
Publication of GEP20033036B publication Critical patent/GEP20033036B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1. Technical Result Quick extraction of prion protein from a biological material. 2. Essence In a specific example, abnormal prion protein is extracted from homogenized sheep brain with hexafluoro-2-propanol. The hexafluoro-2-propanol is separated from the aqueous brain preparation by increasing the ionic strength of the aqueous solution. Prion protein in the organic extract can be further purified, or the extract can be tested, e.g., by immunoassay, for the presence of prion protein, and more particularly abnormal prion protein. The extraction process permits testing for the presence of abnormal prior protein, e.g., for diagnosis of transmissible spongiform encephalopathies (TSE). 3. Field of Application Medicine, veterinary.
GEAP20006037A 1999-01-08 2000-01-07 Method and Kit for Extracting Prion Protein GEP20033036B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11527299P 1999-01-08 1999-01-08
US09/420,850 US6150172A (en) 1999-01-08 1999-10-19 Method and kit for extracting prion protein

Publications (1)

Publication Number Publication Date
GEP20033036B true GEP20033036B (en) 2003-07-25

Family

ID=26813023

Family Applications (1)

Application Number Title Priority Date Filing Date
GEAP20006037A GEP20033036B (en) 1999-01-08 2000-01-07 Method and Kit for Extracting Prion Protein

Country Status (23)

Country Link
US (1) US6150172A (en)
EP (1) EP1147415A4 (en)
JP (1) JP2002534681A (en)
KR (1) KR20010089776A (en)
CN (1) CN1135235C (en)
AU (1) AU761319B2 (en)
BR (1) BR0007274A (en)
CA (1) CA2358285A1 (en)
CZ (1) CZ20012469A3 (en)
EA (1) EA004953B1 (en)
EE (1) EE200100358A (en)
GE (1) GEP20033036B (en)
HK (1) HK1044588B (en)
HU (1) HUP0104890A3 (en)
ID (1) ID29442A (en)
IL (1) IL143974A0 (en)
NO (1) NO20013193L (en)
NZ (1) NZ513215A (en)
OA (1) OA11745A (en)
PL (1) PL349132A1 (en)
SK (1) SK9492001A3 (en)
TR (1) TR200101935T2 (en)
WO (1) WO2000040966A1 (en)

Families Citing this family (46)

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Publication number Priority date Publication date Assignee Title
NZ332132A (en) 1996-04-03 2000-02-28 Stichting Inst Voor Dierhouder Pre-clinical detection of prion diseases such as PrPsc
US6720355B2 (en) 1997-02-21 2004-04-13 The Regents Of The University Of California Sodium dodecyl sulfate compositions for inactivating prions
US6719988B2 (en) 1997-02-21 2004-04-13 The Regents Of The University Of California Antiseptic compositions for inactivating prions
US6322802B1 (en) 1999-06-01 2001-11-27 The Regents Of The University Of California Method of sterilizing
US6331296B1 (en) 1999-06-01 2001-12-18 Stanley B. Prusiner Food additives which affect conformationally altered proteins
US20060008494A1 (en) * 1997-02-21 2006-01-12 The Regents Of The University Of California Complete inactivation of infectious proteins
FR2774988B1 (en) * 1998-02-16 2000-05-05 Commissariat Energie Atomique PROCESS FOR THE PURIFICATION OF PRPRES FROM A BIOLOGICAL SAMPLE AND ITS APPLICATIONS
GB9902659D0 (en) * 1999-02-05 1999-03-31 Microbiological Res Authority Assay with reduced background
WO2000048003A1 (en) 1999-02-11 2000-08-17 Id-Lelystad, Instituut Voor Dierhouderij En Diergezondheid B.V. Prion test
US6605445B1 (en) * 1999-02-22 2003-08-12 Bayer Corporation Rapid method of determining clearance of prion protein
US7041807B1 (en) 1999-06-23 2006-05-09 Caprion Pharmaceuticals, Inc. Antibodies to a YYX epitope of a mammalian prion protein
US6437102B1 (en) * 1999-11-24 2002-08-20 Bayer Corporation Method of separating prions from biological materials
US20030050276A1 (en) * 2001-08-15 2003-03-13 Cunanan Crystal M. Treatment of tissue, instruments and work surfaces to remove infectious agents
IES20010042A2 (en) * 2001-01-19 2002-12-11 Enfer Technology Ltd Test for transmissible spongiform encephalopathies
US20020192731A1 (en) * 2001-04-12 2002-12-19 H. Shih Jason C. Method and composition for sterilizing surgical instruments
WO2009059286A1 (en) * 2007-11-02 2009-05-07 Biotrove, Inc. Devices and methods for coupling mass spectrometry devices with chromatography systems
US8414774B2 (en) * 2001-04-25 2013-04-09 Agilent Technologies, Inc. Systems and methods for high-throughput screening of fluidic samples
US20050123970A1 (en) * 2001-04-25 2005-06-09 Can Ozbal High throughput autosampler
WO2002093168A1 (en) * 2001-05-15 2002-11-21 Gradipore Limited Prion diagnostic test
FR2827047B1 (en) * 2001-07-03 2003-09-26 Apoh Technollgies Sa PROCESS FOR SEPARATION AND / OR DETECTION AND / OR IDENTIFICATION AND / OR QUANTIFICATION OF PRION PROTEINS
US6591991B2 (en) * 2001-08-06 2003-07-15 Luce Belle Collapsible tire stand
US7045297B2 (en) * 2001-11-14 2006-05-16 Prion Developmental Laboratories, Inc. Rapid prion-detection assay
US20030092090A1 (en) * 2001-11-14 2003-05-15 Kiamars Hajizadeh Rapid prion-detection device, system, and test kit
US7659076B2 (en) † 2002-02-28 2010-02-09 Microsens Biophage Limited Binding of pathological forms of prion proteins
AU2003280457A1 (en) * 2002-06-27 2004-01-19 University Of Marlyland, Baltimore Methods for detecting and inactivating a prion
DE10230141B4 (en) * 2002-07-04 2004-07-15 Priontype Gmbh Method and kit for the enrichment and detection of modified prion proteins (PrPSc)
US20040018120A1 (en) * 2002-07-29 2004-01-29 Craig Rappin Sample preparation device and method
US20040018575A1 (en) * 2002-07-29 2004-01-29 Craig Rappin Sample preparation device and method
CH693953A5 (en) * 2002-10-15 2004-05-14 Tecan Trading Ag Enriching protein-containing liquids, comprises diluting liquids before enrichment in five to ten times excess of solution
US20040175775A1 (en) * 2002-11-08 2004-09-09 Neil Cashman Method of detecting PrPsc in eye fluid
AU2003296953A1 (en) * 2002-12-12 2004-07-09 Strategic Diagnostics Inc. Compositions and methods for detecting animal by product in feed
FR2849205B1 (en) * 2002-12-20 2005-02-11 Afssa METHOD FOR AMPLIFYING PRPSC DETECTION AND USE OF A MACROCYCLIC ADJUVANT LIGAND FOR SUCH AMPLIFICATION
US20090068640A1 (en) * 2003-06-27 2009-03-12 Robert Rohwer Methods for detecting and inactivating a prion
US20050054003A1 (en) * 2003-09-10 2005-03-10 Stenland Christopher J. Prion clearance using particulate metal oxides
DE10354207B8 (en) * 2003-11-20 2006-06-14 Priontype Gmbh & Co.Kg Method for the detection of pathologically altered prion proteins (PrPSc) and kit for carrying out the method
JP4724816B2 (en) * 2005-09-06 2011-07-13 シャープ株式会社 Protein measurement method
DE102006044795A1 (en) * 2006-09-13 2008-03-27 Schweigert, Florian, Prof. Dr.med.vet. Determination of ingredients from biological materials
CA2689145A1 (en) * 2007-06-04 2008-12-11 Pressure Biosciences Inc. Pressure-enhanced extraction and partitioning of molecules
US20120156794A1 (en) * 2008-03-19 2012-06-21 Florian Schweigert Method for the extraction and detection of fat-soluble components from biological materials
EP2288696A4 (en) * 2008-05-07 2012-02-15 Pressure Biosciences Inc Extraction of biomolecular complexes assisted by alternating hydrostatic pressure
HRP20192259T1 (en) 2009-04-15 2020-03-06 Abraxis Bioscience, Llc PREPARATIONS OF NON-PARTICLES WITHOUT PRION AND METHODS OF THEIR PRODUCTION
CN103255142B (en) * 2012-02-21 2017-07-25 上海转基因研究中心 A kind of method and its application of utilization RNAi regulation and control endogenous PrPC expression
CN102757493A (en) * 2012-07-11 2012-10-31 北京健翔和牧生物科技有限公司 Method for preparing chicken pox virus antibody
US20200276518A1 (en) * 2017-11-06 2020-09-03 Shimadzu Corporation Component separation method using supercritical fluid chromatograph
US11808675B2 (en) * 2019-06-13 2023-11-07 Agilent Technologies, Inc. Room temperature methods for preparing biological analytes
EP4056175A4 (en) * 2019-11-08 2023-12-06 Central Glass Company, Limited Method for selectively killing protein aggregate-containing cells, kit therefor, therapeutic drug for protein misfolding diseases and drug product for removing protein aggregates from blood product

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US6020537A (en) * 1994-05-13 2000-02-01 The Regents Of The University Of California Prion protein standard and method of making same
US5998149A (en) * 1996-04-05 1999-12-07 The United States Of America As Represented By The Department Of Health And Human Services Method of detecting transmissible spongiform encephalopathies
US5808011A (en) * 1996-07-01 1998-09-15 Biopure Corporation Method for chromatographic removal of prions

Also Published As

Publication number Publication date
OA11745A (en) 2005-05-13
JP2002534681A (en) 2002-10-15
HUP0104890A3 (en) 2003-09-29
CA2358285A1 (en) 2000-07-13
EA004953B1 (en) 2004-10-28
NZ513215A (en) 2002-12-20
EP1147415A4 (en) 2004-06-30
IL143974A0 (en) 2002-04-21
HK1044588A1 (en) 2002-10-25
TR200101935T2 (en) 2002-01-21
CN1135235C (en) 2004-01-21
AU761319B2 (en) 2003-06-05
HK1044588B (en) 2004-12-03
KR20010089776A (en) 2001-10-08
CZ20012469A3 (en) 2002-02-13
NO20013193L (en) 2001-08-15
EE200100358A (en) 2002-12-16
BR0007274A (en) 2001-10-30
WO2000040966A1 (en) 2000-07-13
CN1342266A (en) 2002-03-27
ID29442A (en) 2001-08-30
HUP0104890A2 (en) 2002-04-29
AU2604300A (en) 2000-07-24
EA200100756A1 (en) 2001-12-24
SK9492001A3 (en) 2002-06-04
PL349132A1 (en) 2002-07-01
NO20013193D0 (en) 2001-06-25
US6150172A (en) 2000-11-21
EP1147415A1 (en) 2001-10-24

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