HK65497A - Cdnas encoding somatotropin, expression vectors and hosts - Google Patents

Cdnas encoding somatotropin, expression vectors and hosts Download PDF

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Publication number
HK65497A
HK65497A HK65497A HK65497A HK65497A HK 65497 A HK65497 A HK 65497A HK 65497 A HK65497 A HK 65497A HK 65497 A HK65497 A HK 65497A HK 65497 A HK65497 A HK 65497A
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HK
Hong Kong
Prior art keywords
plasmid
gene
bst
expression
fragment
Prior art date
Application number
HK65497A
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English (en)
French (fr)
Inventor
Chiu Tomich Che-Shen
Eric R. Olson
John E. Mott
Original Assignee
Pharmacia & Upjohn Company
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Publication date
Application filed by Pharmacia & Upjohn Company filed Critical Pharmacia & Upjohn Company
Publication of HK65497A publication Critical patent/HK65497A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • C07K14/592—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH] at least 1 amino acid in D-form
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Reproductive Health (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Radio Relay Systems (AREA)
  • Compression, Expansion, Code Conversion, And Decoders (AREA)
  • Cosmetics (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Two-Way Televisions, Distribution Of Moving Picture Or The Like (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Compression Or Coding Systems Of Tv Signals (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)

Claims (11)

  1. Expressionsplasmid, welches eine E.coli-Wirtzelle zu transformieren vermag und die Wirtzelle ein heterologes Protein produzieren läßt, umfassend ein Mutanten-R1-Replikon aus pBEU-17 oder pBEU-50, einen Replikationsursprung aus pBR322 und eine cDNA mit Codierung für das heterologe Protein.
  2. Plasmid nach Anspruch 1, wobei das heterologe Protein aus Schweinesomatotropin besteht.
  3. Plasmid nach Anspruch 1, wobei das heterologe Protein aus Rindersomatotropin besteht.
  4. Plasmid nach Anspruch 2 oder 3, wobei die ersten vier Kodons der cDNA mit Kodierung für das Somatotropin aus GCC TTC CCA GCT, GCT TTC CCA GCT, ACC TTC CCA GCC, GCC TTC CCA GTC, AAA TTC CCA GCC, TTC TTC CCA GCC und TTC CCA GCC ATG ausgewählt sind.
  5. Plasmid nach Anspruch 3 und 4, wobei die ersten vier Kodons der cDNA aus GCC TTC CCA GCT bestehen.
  6. E.coli-Wirtzelle, umfassend eine Mutation des rpoH-Gens und ein Plasmid nach einem der Ansprüche 1 bis 5.
  7. Wirtzelle nach Anspruch 6, wobei das mutierte Gen aus dem rpoH112 Allel besteht.
  8. E.coli-Wirtzelle, umfassend eine Mutation des hf1B-Gens und ein Plasmid nach einem der Ansprüche 1 bis 5.
  9. Wirtzelle nach Anspruch 6, wobei das mutierte Gen aus dem rf1B29 Allel besteht.
  10. Verwendung einer Wirtzelle nach einem der Ansprüche 6 bis 9 zur Expression des Gens für das heterologe Protein.
  11. E.coli-Wirtzelle BST-1C (NRRL B-18303) oder D112 (NRRL B-18168).
HK65497A 1987-02-19 1997-05-15 Cdnas encoding somatotropin, expression vectors and hosts HK65497A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1629487A 1987-02-19 1987-02-19
PCT/US1988/000328 WO1988006186A2 (en) 1987-02-19 1988-01-27 cDNAs ENCODING SOMATOTROPIN, EXPRESSION VECTORS AND HOSTS

Publications (1)

Publication Number Publication Date
HK65497A true HK65497A (en) 1997-05-23

Family

ID=21776383

Family Applications (1)

Application Number Title Priority Date Filing Date
HK65497A HK65497A (en) 1987-02-19 1997-05-15 Cdnas encoding somatotropin, expression vectors and hosts

Country Status (9)

Country Link
EP (1) EP0418219B1 (de)
JP (1) JP2580303B2 (de)
KR (1) KR960016704B1 (de)
AT (1) ATE107696T1 (de)
AU (1) AU615432B2 (de)
DE (1) DE3850410T2 (de)
DK (1) DK580188A (de)
HK (1) HK65497A (de)
WO (1) WO1988006186A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104806A (en) * 1987-03-12 1992-04-14 Amgen, Inc. Porcine growth hormone analogs encoding dna
AU3183289A (en) * 1988-01-27 1989-08-25 Upjohn Company, The Expression vectors
US5268284A (en) * 1988-03-11 1993-12-07 The Upjohn Company Ribosome binding site
WO1990005186A1 (en) * 1988-11-04 1990-05-17 The Upjohn Company Somatotropin expression using a serratia promoter
AU643752B2 (en) * 1990-01-31 1993-11-25 Upjohn Company, The Strain RAL-4, useful for production of small proteins and peptides

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ201918A (en) * 1981-09-18 1987-04-30 Genentech Inc N-terminal methionyl analogues of bovine growth hormone
EP0111814A3 (de) * 1982-12-16 1985-08-14 Mgi Pharma, Inc. Klonierung und Expression von nucleotiden Sequenzen die für Rinderwachstumshormon kodieren
GB8308236D0 (en) * 1983-03-25 1983-05-05 Celltech Ltd Dna vectors
JPS6011557A (ja) * 1983-04-19 1985-01-21 ジエネツクス・コ−ポレイシヨン クロ−ンヒツジ成長ホルモン遺伝子
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US4710473A (en) * 1983-08-10 1987-12-01 Amgen, Inc. DNA plasmids
AU557486B2 (en) * 1984-01-27 1986-12-24 Monsanto Company Composition for improved bovine feeding and milk production
US4758512A (en) * 1984-03-06 1988-07-19 President And Fellows Of Harvard College Hosts and methods for producing recombinant products in high yields
EP0159123B1 (de) * 1984-03-06 1992-01-15 Eli Lilly And Company Expressionsvektoren für Rinderwachstumshormonderivate
EP0355460B1 (de) * 1988-08-24 2000-12-27 American Cyanamid Company Stabilisierung von Somatotropinen durch Modifikation von Cystein-Resten durch orts-spezifische Mutagenese oder chemische Derivatisierung

Also Published As

Publication number Publication date
WO1988006186A2 (en) 1988-08-25
JP2580303B2 (ja) 1997-02-12
JPH02502245A (ja) 1990-07-26
WO1988006186A3 (en) 1988-11-03
DE3850410T2 (de) 1994-10-27
KR960016704B1 (ko) 1996-12-20
KR890700663A (ko) 1989-04-26
DK580188D0 (da) 1988-10-18
EP0418219B1 (de) 1994-06-22
AU1363988A (en) 1988-09-14
EP0418219A1 (de) 1991-03-27
DK580188A (da) 1988-10-18
DE3850410D1 (de) 1994-07-28
ATE107696T1 (de) 1994-07-15
AU615432B2 (en) 1991-10-03

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PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20070209