HRP20121048T1 - Reverzna genetika korištenjem ne-endogenih pol i promotora - Google Patents

Reverzna genetika korištenjem ne-endogenih pol i promotora Download PDF

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HRP20121048T1
HRP20121048T1 HRP20121048AT HRP20121048T HRP20121048T1 HR P20121048 T1 HRP20121048 T1 HR P20121048T1 HR P20121048A T HRP20121048A T HR P20121048AT HR P20121048 T HRP20121048 T HR P20121048T HR P20121048 T1 HRP20121048 T1 HR P20121048T1
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virus
promoter
pol
expression
cell
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HRP20121048AT
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Philip Dormitzer
Michael Franti
Pirada Suphaphiphat
Peter Mason
Bjoern Keiner
Stephania Crotta
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Novartis Ag
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Publication of HRP20121048T1 publication Critical patent/HRP20121048T1/hr

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Claims (15)

1. Postupak za dobivanje rekombinantnog virusa, koji da sadrži korak uzgoja pseće stanice domaćina koja sadrži barem jedan ekspresijski konstrukt koji kodira virusnu RNK molekulu, naznačen time da je ekspresija virusne RNK molekule iz konstrukta kontrolirana pomoću pol I promotora od primata pod uvjetima da je eksprimirana virusna RNK molekula kako bi se proizveo virus.
2. Postupak za dobivanje virusa, naznačen time da sadrži korake: (i) proizvodnje rekombinantnog virusa postupkom prema zahtjevu 1; (ii) infekcije kultiviranog domaćina sa virusom dobivenim u koraku (i); (iii) uzgoja domaćina iz koraka (ii) da se proizvede dodatni virus; i (iv) pročišćavanja virusa dobivenog u koraku (iii).
3. Postupak za dobivanje cjepiva, naznačen time da sadrži korake (a) pripreme virusa postupkom prema zahtjevu 2 i (b) pripreme cjepiva od virusa.
4. Postupak prema bilo kojem prethodnom zahtjevu naznačen time da pol I promotor je ljudski pol I promotor.
5. Postupak prema bilo kojem prethodnom zahtjevu naznačen time da stanica je MDCK stanica.
6. Postupak prema zahtjevu 5 naznačen time da MDCK stanica je stanična linija MDCK 33016 (DSM ACC2219).
7. Postupak prema bilo kojem prethodnom zahtjevu naznačen time da stanica sadrži barem jedan dvosmjerni ekspresijski konstrukt.
8. Postupak prema bilo kojem prethodnom zahtjevu naznačen time da ekspresijski konstrukt je ekspresijski vektor ili linearni ekspresijski konstrukt.
9. Postupak prema bilo kojem od zahtjeva 4 do 8, naznačen time da ljudski pol I promotor sadrži sekvencu SEQ ID NO: 1 ili SEQ ID NO: 2.
10. Postupak prema bilo kojem prethodnom zahtjevu naznačen time da virus je segmentirani virus.
11. Postupak prema bilo kojem prethodnom zahtjevu naznačen time da virus je ne- segmentirani virus.
12. Postupak prema bilo kojem prethodnom zahtjevu naznačen time da virus je negativni RNK virus.
13. Postupak prema zahtjevu 12 naznačen time da virus je virus influence.
14. Pseća stanica domaćin koja proizvodi virus, koja sadrži barem jedan ekspresijski konstrukt koji kodira virusnu RNK molekulu, naznačena time da je ekspresija virusne RNK molekule iz konstrukta kontrolirana pomoću pol I promotora od primata.
15. Pseća stanica koja proizvodi virus, naznačena time da ima barem jedan endogeni pol I promotor koji kontrolira(ju) ekspresiju endogene rRNK i barem jedan ne-endogeni pol I promotor primata koji kontrolira(ju) ekspresiju virusne RNK ili njezin komplement.
HRP20121048AT 2009-05-21 2010-05-21 Reverzna genetika korištenjem ne-endogenih pol i promotora HRP20121048T1 (hr)

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US21691909P 2009-05-21 2009-05-21
PCT/IB2010/001332 WO2010133964A1 (en) 2009-05-21 2010-05-21 Reverse genetics using non-endogenous pol i promoters

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US (3) US9072702B2 (hr)
EP (2) EP2401384B1 (hr)
JP (2) JP5836266B2 (hr)
KR (1) KR101800245B1 (hr)
CN (1) CN102597246B (hr)
AU (1) AU2010250832B2 (hr)
CA (1) CA2762802A1 (hr)
DK (1) DK2401384T3 (hr)
EA (1) EA021009B1 (hr)
ES (1) ES2394797T3 (hr)
HR (1) HRP20121048T1 (hr)
NZ (1) NZ596432A (hr)
PL (1) PL2401384T3 (hr)
PT (1) PT2401384E (hr)
SI (1) SI2401384T1 (hr)
SM (1) SMT201300034B (hr)
WO (1) WO2010133964A1 (hr)

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ES2394797T3 (es) 2013-02-05
DK2401384T3 (da) 2013-01-14
US20120189656A1 (en) 2012-07-26
EA021009B1 (ru) 2015-03-31
AU2010250832A1 (en) 2011-12-08
EP2401384A1 (en) 2012-01-04
KR101800245B1 (ko) 2017-11-22
NZ596432A (en) 2013-05-31
KR20120028328A (ko) 2012-03-22
US9072702B2 (en) 2015-07-07
AU2010250832B2 (en) 2013-10-03
CN102597246A (zh) 2012-07-18
JP2015204843A (ja) 2015-11-19
PT2401384E (pt) 2012-12-19
PL2401384T3 (pl) 2013-03-29
US20160024525A1 (en) 2016-01-28
BRPI1011171A2 (pt) 2015-08-25
EA201171449A1 (ru) 2012-05-30
EP2401384B1 (en) 2012-10-03
US20190284575A1 (en) 2019-09-19
WO2010133964A1 (en) 2010-11-25
EP2573184A1 (en) 2013-03-27
SI2401384T1 (sl) 2013-01-31
HK1165827A1 (en) 2012-10-12
CN102597246B (zh) 2014-02-26
JP2012527228A (ja) 2012-11-08
JP5836266B2 (ja) 2015-12-24
CA2762802A1 (en) 2010-11-25

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