HRP20171955T1 - Suncokreti otporni na herbicide s više alela ahasl1 otpornih na herbicide i postupci njihove upotrebe - Google Patents

Suncokreti otporni na herbicide s više alela ahasl1 otpornih na herbicide i postupci njihove upotrebe Download PDF

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HRP20171955T1
HRP20171955T1 HRP20171955TT HRP20171955T HRP20171955T1 HR P20171955 T1 HRP20171955 T1 HR P20171955T1 HR P20171955T T HRP20171955T T HR P20171955TT HR P20171955 T HRP20171955 T HR P20171955T HR P20171955 T1 HRP20171955 T1 HR P20171955T1
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herbicide
ahasl1
methyl
sunflower
seq
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HRP20171955TT
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Carlos Sala
Mariano Bulos
Mariel Echarte
Bijay K. Singh
Brigitte J. Weston
Sherry R. Whitt
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Basf Agrochemical Products, B.V.
Nidera Semillas S.A.
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Publication of HRP20171955T1 publication Critical patent/HRP20171955T1/hr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/02—Flowers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A01H1/123—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14—Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278—Sulfonylurea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
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  • Natural Medicines & Medicinal Plants (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Catching Or Destruction (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (22)

1. Suncokret otporan na herbicide, naznačen time što sadrži dva različita alela otporna na herbicide gena za veliku podjedinicu 1 suncokretske sintaze acetohidroksikiselina (AHASL1), gdje prvi alel kodira prvi protein AHASL1 otporan na herbicide, koji sadrži zamjenu alanina treoninom na aminokiselinskom položaju koji odgovara položaju 7 u SEQ ID NO:20, a drugi alel kodira drugi protein AHASL1 otporan na herbicide, koji sadrži zamjenu alanina valinom na aminokiselinskom položaju koji odgovara položaju 90 u SEQ ID NO:20 ili zamjenu prolina leucinom na aminokiselinskom položaju koji odgovara položaju 82 u SEQ ID NO:20; gdje je navedeni suncokret otporan na herbicide otporan na najmanje jedan herbicid koji inhibira AHAS.
2. Suncokret u skladu s patentnim zahtjevom 1, naznačen time što je navedeni herbicid koji inhibira AHAS imidazolinonski herbicid, sulfonilurejski herbicid, triazolopirimidinski herbicid, sulfonilamino-karboniltriazolinonski herbicid, ili njihove smjese.
3. Suncokret u skladu s patentnim zahtjevom 1, naznačen time što je navedeni suncokret u obliku sjemena.
4. Suncokret u skladu s patentnim zahtjevom 1, naznačen time što navedeni suncokret proizvodi sjemenke koje sadrže ulje koje se može ekstrahirati iz sjemenki koje sadrži najmanje 85% oleinske kiseline.
5. Postupak uzgoja suncokreta prilikom kontrole korova u blizini navedenog suncokreta, gdje se navedeni postupak sastoji u primjeni djelotvorne količina herbicide koji inhibira AHAS na korovima i na suncokretu, naznačen time što se navedeni suncokret bira iz skupine koju čine: (a) suncokret koji sadrži prvi alel gena AHASL1 i drugi alel navedenog gena AHASL1, gdje navedeni prvi alel kodira prvi protein AHASL1 otporan na herbicide, koji sadrži zamjenu alanina treoninom na aminokiselinskom položaju koji odgovara položaju 7 u SEQ ID NO:20, a navedeni drugi alel kodira drugi protein AHASL1 otporan na herbicide, koji sadrži zamjenu alanina valinom na aminokiselinskom položaju koji odgovara položaju 90 u SEQ ID NO:20; i (b) suncokret koji sadrži prvi alel gena AHASL1 i drugi alel navedenog gena AHASL1, gdje navedeni prvi alel kodira prvi protein AHASL1 otporan na herbicide, koji sadrži zamjenu alanina treoninom na aminokiselinskom položaju koji odgovara položaju 7 u SEQ ID NO:20, a navedeni drugi alel kodira drugi protein AHASL1 otporan na herbicide, koji sadrži zamjena prolina leucinom na aminokiselinskom položaju koji odgovara položaju 82 u SEQ ID NO:20.
6. Postupak u skladu s patentnim zahtjevom 5, naznačen time što navedeni herbicid koji inhibira AHAS sadrži imidazolinonski herbicid, sulfonilurejski herbicid, triazolopirimidinski herbicid, sulfonilamino-karboniltriazolinonski herbicid, ili njihova smjesa.
7. Postupak u skladu s patentnim zahtjevom 6, naznačen time što navedeni imidazolinonski herbicid sadrži: 2-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)nikotinsku kiselinu, 2-(4-izopropil)-4-metil-5-okso-2-imidazolin-2-il)kinolin-3-karboksilnu kiselinu, 5-etil-2-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)nikotinsku kiselinu, 2-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)-5-(metoksimetil)nikotinsku kiselinu, 2-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)-5-metilnikotinsku kiselinu, smjese metil-6-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)-m-toluata i metil-2-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)-p-toluata, ili njihove smjese.
8. Postupak u skladu s patentnim zahtjevom 6, naznačen time što navedeni sulfonilurejski herbicid sadrži: klorsulfuron, metsulfuron-metil, sulfometuron-metil, klorimuron-etil, tifensulfuron-metil, tribenuron-metil, bensulfuron-metil, nicosulfuron, etametsulfuron-metil, rimsulfuron, triflusulfuron-metil, triasulfuron, primisulfuron-metil, cinosulfuron, amidosulfuron, flazasulfuron, imazosulfuron, pirazosulfuron-etil, halosulfuron, ili njihove smjese.
9. Sjeme suncokreta u skladu s patentnim zahtjevom 3, naznačeno time što se navedeno sjeme tretira herbicidom koji inhibira AHAS.
10. Sjeme u skladu s patentnim zahtjevom 9, naznačeno time što navedeni herbicid koji inhibira AHAS sadrži imidazolinonski herbicid, sulfonilurejski herbicid, triazolopirimidinski herbicid, sulfonilamino-karboniltriazolinonski herbicid, ili njihovu smjesu.
11. Postupak uzgoja suncokreta otpornog na herbicide u skladu s patentnim zahtjevom 1 prilikom borbe protiv nepoželjnog raslinja, naznačen time što se sastoji u stavljanju u kontakt sjemena suncokreta prije sjetve i/ili nakon predklijanja djelotvornom količinom herbicida koji inhibira AHAS, gdje navedeno sjeme suncokreta sadrži dva različita alela otporna na herbicide gena za veliku podjedinicu 1 suncokretske sintaze acetohidroksikiselina (AHASL1), gdje prvi alel kodira prvi protein AHASL1 otporan na herbicide, koji sadrži zamjenu alanina treoninom na aminokiselinskom položaju koji odgovara položaju 7 u SEQ ID NO:20, a drugi alel kodira drugi protein AHASL1 otporan na herbicide, koji sadrži zamjenu alanina valinom na aminokiselinskom položaju koji odgovara položaju 90 u SEQ ID NO:20 ili zamjenu prolina leucinom na aminokiselinskom položaju koji odgovara položaju 82 u SEQ ID NO:20.
12. Postupak u skladu s patentnim zahtjevom 11, naznačen time što navedeni herbicid koji inhibira AHAS sadrži imidazolinonski herbicid, sulfonilurejski herbicid, triazolopirimidinski herbicid, sulfonilamino-karboniltriazolinonski herbicid, ili njihovu smjesu.
13. Postupak u skladu s patentnim zahtjevom 5, naznačen time što navedeni korovi uključuju volovod.
14. Postupak u skladu s patentnim zahtjevom 13, naznačen time što se navedeni volovod bira iz skupine koju čine: Orobanche cumana i Orobanche cernua.
15. Postupak u skladu s patentnim zahtjevom 13, naznačen time što navedeni imidazolinonski herbicid sadrži: 2-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)nikotinsku kiselinu, 2-(4-izopropil)-4-metil-5-okso-2-imidazolin-2-il)-3-kinolinkarboksilnu kiselinu, 5-etil-2-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)nikotinsku kiselinu, 2-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)-5-(metoksimetil)nikotinsku kiselinu, 2-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)-5-metilnikotinsku kiselinu, smjese metil-6-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)-m-toluata i metil-2-(4-izopropil-4-metil-5-okso-2-imidazolin-2-il)-p-toluata, ili njihove smjese.
16. Postupak u skladu s patentnim zahtjevom 13, naznačen time što je navedeni imidazolinonski herbicid imazapir.
17. Postupak u skladu s patentnim zahtjevom 13, naznačen time što se navedeni imidazolinonski herbicid primjenjuje u stadiju rasta R1.
18. Postupak genotipiziranja suncokreta u skladu s bilo kojim od patentnih zahtjeva 1-4, ili njegovog potomstva, ili sjemena u skladu s bilo kojim od patentnih zahtjeva 9-10, što sadrži suncokretski AHASL1, naznačen time što se navedeni postupak sastoji u koracima: (a) dobivanja genomske DNA iz navedenog suncokreta ili sjemena; (b) upotrebe navedene DNA kao uzorka za prvu amplifikaciju lančanom reakcijom polimeraze (PCR), koja uključuje navedenu DNA, polimerazu, deosiribonukleotid-trifosfate, početnicu prema naprijed za AHASL1 i početnicu prema natrag za divlji tip AHASL1, gdje se navedena početnica prema natrag za divlji tip AHASL1 sparuje s nukleotidnim slijedom koji sadrži nukleotidni slijed iznijet u SEQ ID NO:13, gdje je nukleotid koji je na 3′ kraju nukleotid navedene početnice prema natrag za divlji tip AHASL1 koji je komplement nukleotida koji je na položaju 1 u nukleotidnom slijedu iznijetom u SEQ ID NO:13; (c) upotreba navedene DNA kao uzorak za drugu PCR amplifikaciju koja uključuje navedenu DNA, polimerazu, deosiribonukleotid-trifosfate, navedenu početnicu prema naprijed za AHASL1 i početnicu prema natrag za mutantni AHASL1, gdje se navedena početnica prema natrag za mutantni AHASL1 sparuje s nukleotidnim slijedom koji sadrži nukleotidni slijed iznijet u SEQ ID NO:14, gdje je nukleotid koji je na 3′ kraju nukleotid navedene početnice prema natrag za mutantni AHASL1 koji je komplement nukleotida koji je na položaju 1 u nukleotidnom slijedu iznijetom u SEQ ID NO:14; i (d) detektiranja produkata navedene prve i navedene druge PCR amplifikacije; gdje navedena početnica prema naprijed za AHASL1 sadrži nukleotidni slijed koji odgovara području suncokretskog gena AHASL1 koje je 5′ u odnosu na područje (ACC)n.
19. Postupak u skladu s patentnim zahtjevom 18, naznačen time što navedena početnica prema natrag za divlji tip AHASL1 sadrži nukleotidni slijed iznijet u SEQ ID NO:4.
20. Postupak u skladu s patentnim zahtjevom 18, naznačen time što navedena početnica prema natrag za mutantni AHASL1 sadrži nukleotidni slijed iznijet u SEQ ID NO:5.
21. Postupak u skladu s patentnim zahtjevom 18, naznačen time što se navedena početnica prema naprijed za AHASL1 sparuje s nukleotidnim slijedom koji sadrži komplement nukleotidnog slijeda iznijetog u SEQ ID NO:12.
22. Postupak u skladu s patentnim zahtjevom 18, naznačen time što navedena početnica prema naprijed za AHASL1 sadrži nukleotidni slijed iznijet u SEQ ID NO:3.
HRP20171955TT 2007-04-04 2008-04-02 Suncokreti otporni na herbicide s više alela ahasl1 otpornih na herbicide i postupci njihove upotrebe HRP20171955T1 (hr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US91004107P 2007-04-04 2007-04-04
US2973708P 2008-02-19 2008-02-19
PCT/US2008/059125 WO2008124431A1 (en) 2007-04-04 2008-04-02 Herbicide-resistant sunflower plants with multiple herbicide resistant alleles of ahasl1 and methods of use
EP08744931.0A EP2134839B8 (en) 2007-04-04 2008-04-02 Herbicide-resistant sunflower plants with multiple herbicide resistant alleles of ahasl1 and methods of use

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US (3) US20110277051A1 (hr)
EP (1) EP2134839B8 (hr)
JP (2) JP5715414B2 (hr)
CN (2) CN101878300B (hr)
AR (1) AR065953A1 (hr)
AU (1) AU2008237436B2 (hr)
CA (1) CA2682554C (hr)
CL (2) CL2008000975A1 (hr)
EA (2) EA023348B1 (hr)
ES (1) ES2659991T3 (hr)
HR (1) HRP20171955T1 (hr)
PT (1) PT2134839T (hr)
SI (1) SI2134839T1 (hr)
UA (1) UA102223C2 (hr)
WO (1) WO2008124431A1 (hr)
ZA (1) ZA200907701B (hr)

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