HRP20200062T1 - Postupak proučavanja nukleinskih kiselina - Google Patents
Postupak proučavanja nukleinskih kiselina Download PDFInfo
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- HRP20200062T1 HRP20200062T1 HRP20200062TT HRP20200062T HRP20200062T1 HR P20200062 T1 HRP20200062 T1 HR P20200062T1 HR P20200062T T HRP20200062T T HR P20200062TT HR P20200062 T HRP20200062 T HR P20200062T HR P20200062 T1 HRP20200062 T1 HR P20200062T1
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- Prior art keywords
- cells
- nucleic acid
- chromatin
- agent
- transposase
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/04—Methods of creating libraries, e.g. combinatorial synthesis using dynamic combinatorial chemistry techniques
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/327—RNAse, e.g. RNAseH
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
- C12Q2522/10—Nucleic acid binding proteins
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Structural Engineering (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Claims (15)
1. Postupak priprave biblioteke sekvenciranja, naznačen time što se postupak sastoji u:
(a) dodavanju sredstva koje se veže na kromatin u uzorak koji sadrži nukleinsku kiselinu;
(b) izoliranju kromatina vezanog na navedeno sredstvo;
(c) dodavanju transpozaze izoliranom kromatinu iz koraka (b);
(d) izoliranju nukleinske kiseline iz kromatina; i
(e) dobivanju biblioteke sekvenciranja.
2. Postupak kartiranja molekulskih međudjelovanja koja uključuju nukleinsku kiselinu, naznačen time što se postupak sastoji u:
(a) dodavanju sredstva koje se veže na kromatin u uzorak koji sadrži nukleinsku kiselinu;
(b) izoliranju kromatina vezanog na navedeno sredstvo;
(c) dodavanju transpozaze izoliranom kromatinu iz koraka (b);
(d) izoliranju nukleinske kiseline iz kromatina;
(e) amplificiranju nukleinske kiseline;
(f) sekvenciranju amplificirane nukleinske kiseline; i
(g) identificiranju molekulskih međudjelovanja.
3. Postupak u skladu s patentnim zahtjevima 1 ili 2, naznačen time što je sredstvo protutijelo.
4. Postupak u skladu s patentnim zahtjevima 1 do 3, naznačen time što se uzorak koji sadrži nukleinsku kiselinu pripravlja
(i) uzgajanjem i prikupljanjem stanica;
(ii) fiksiranjem stanica;
(iii) liziranjem stanica, a time i dobivanjem prvog uzorka koji sadrži nukleinsku kiselinu; i
(iv) sonificiranjem prvog uzorka, a time i dobivanje drugog uzorka koji sadrži nukleinsku kiselinu, gdje
se navedeni drugi uzorak upotrebljava u postupku u skladu s patentnim zahtjevima 1 ili 2.
5. Postupak u skladu s patentnim zahtjevima 1 do 4, naznačen time što postupak dodatno uključuje korak prekidanja veza za umrežavanje unesenih tijekom fiksiranja stanica.
6. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 5, naznačen time što je nukleinska kiselina DNA.
7. Postupak u skladu s bilo kojim od patentnih zahtjeva 4 do 6, naznačen time što stanice sadrže komplekse nukleinska kiselina-protein.
8. Postupak u skladu s patentnim zahtjevom 7, naznačen time što su stanice ljudske stanice, životinjske stanice, bakterijske stanice, stanice kvasca, arhejske stanice, biljne stanice ili virusi.
9. Postupak u skladu s patentnim zahtjevom 8, naznačen time što ljudske ili životinjske stanice su bolesne stanice ili nebolesne stanice ili stanice dobivene iz bolesnog ili nebolesnog tkiva i/ili što ljudske ili životinjske stanice su stanice raka, imunosne stanice, krvne stanice ili matične stanice.
10. Postupak u skladu s patentnim zahtjevom 9, naznačen time što je rak čvrsti rak ili krvni rak, po mogućnosti što je krvni rak leukemija ili što je čvrsti rak tumor.
11. Postupak u skladu s patentnim zahtjevom 4, naznačen time što korak (ii) se sastoji u dodavanju kemijske tvari i/ili fizičkog sredstva i/ili što korak (iv) se sastoji u sonificiranju dok većina fragmenata nukleinske kiseline ne bude duga 20-5000, po mogućnosti 200-300, parova baza.
12. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što se protutijelo specifično veže na histone, transkripcijski čimbenici ili proteini koji se vežu na histone i/ili transkripcijske čimbenike.
13. Postupak u skladu s patentnim zahtjevom 12, naznačen time što su proteini koji se vežu na histone i/ili transkripcijske čimbenike proteini za remodeliranje nukleinskih kiselina ili enzimi za modificiranje kromatina, po mogućnosti što je histon H3.3, H2A.Z, CENP-A, H3.2, H3.3A, H3.3B, H4 ili H3.1, po mogućnosti što je histon modificirani histon, gdje je modificiranje metiliranje, acetiliranje, propioniliranje, butiriliranje, krotoniliranje, 2-hidroksiizobutiriliranje, maloniliranje, sukciniliranje i/ili riboziliranje, po mogućnosti što je modificirani histon H3K4me1/2/3, H2BK5me1, H3K27me1/2/3, H3K9me1/2/3, H4K20me1, H3K79mel, H3K36me3, H2AK5ac, H2AK9ac, H2BK5ac, H2BK12ac, H2BK20ac, H2BK120ac, H3K4ac, H3K9ac, H3K14ac, H3K18ac, H3K23ac, H3K27ac, H3K36ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac, H4K91ac, H2Aub ili H2Bub.
14. Postupak u skladu s patentnim zahtjevima 1 ili 2, naznačen time što je sredstvo kemijska tvar, po mogućnosti što je kemijska tvar lijek, po mogućnosti što je kemijska tvar biotinilirana.
15. Postupak u skladu s patentnim zahtjevima 1 ili 2, naznačen time što transpozaza sadrži nasumične sljedove DNA kao biljege ili definirane sljedove DNA kao biljege, po mogućnosti što je transpozaza transpozaza Tn5.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15180705 | 2015-08-12 | ||
| EP15189788 | 2015-10-14 | ||
| PCT/EP2016/069121 WO2017025594A1 (en) | 2015-08-12 | 2016-08-11 | Methods for studying nucleic acids |
| EP16760659.9A EP3334841B1 (en) | 2015-08-12 | 2016-08-11 | Methods for studying nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20200062T1 true HRP20200062T1 (hr) | 2020-04-03 |
Family
ID=56876995
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20200062TT HRP20200062T1 (hr) | 2015-08-12 | 2016-08-11 | Postupak proučavanja nukleinskih kiselina |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US10934636B2 (hr) |
| EP (2) | EP3334841B1 (hr) |
| JP (1) | JP6697070B2 (hr) |
| CN (1) | CN108368540B (hr) |
| CA (1) | CA2995305A1 (hr) |
| CY (1) | CY1122632T1 (hr) |
| DK (1) | DK3334841T3 (hr) |
| ES (1) | ES2779798T3 (hr) |
| HR (1) | HRP20200062T1 (hr) |
| HU (1) | HUE047175T2 (hr) |
| LT (1) | LT3334841T (hr) |
| PL (1) | PL3334841T3 (hr) |
| PT (1) | PT3334841T (hr) |
| RS (1) | RS59838B1 (hr) |
| SI (1) | SI3334841T1 (hr) |
| SM (1) | SMT202000037T1 (hr) |
| WO (1) | WO2017025594A1 (hr) |
Families Citing this family (86)
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| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
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| US6159736A (en) | 1998-09-23 | 2000-12-12 | Wisconsin Alumni Research Foundation | Method for making insertional mutations using a Tn5 synaptic complex |
| US6406896B1 (en) | 1999-08-02 | 2002-06-18 | Wisconsin Alumni Research Foundation | Transposase enzyme and method for use |
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| JP2018532373A (ja) | 2018-11-08 |
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| CY1122632T1 (el) | 2021-03-12 |
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| CA2995305A1 (en) | 2017-02-16 |
| CN108368540A (zh) | 2018-08-03 |
| US20180237951A1 (en) | 2018-08-23 |
| HUE047175T2 (hu) | 2020-04-28 |
| EP3674414A1 (en) | 2020-07-01 |
| EP3334841B1 (en) | 2019-10-30 |
| JP6697070B2 (ja) | 2020-05-20 |
| EP3334841A1 (en) | 2018-06-20 |
| PL3334841T3 (pl) | 2020-05-18 |
| PT3334841T (pt) | 2020-01-30 |
| RS59838B1 (sr) | 2020-02-28 |
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