HRP20211237T1 - Varijante egzoglukanaza s poboljšanom aktivnošću i njihova upotreba - Google Patents
Varijante egzoglukanaza s poboljšanom aktivnošću i njihova upotreba Download PDFInfo
- Publication number
- HRP20211237T1 HRP20211237T1 HRP20211237TT HRP20211237T HRP20211237T1 HR P20211237 T1 HRP20211237 T1 HR P20211237T1 HR P20211237T T HRP20211237T T HR P20211237TT HR P20211237 T HRP20211237 T HR P20211237T HR P20211237 T1 HRP20211237 T1 HR P20211237T1
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- Prior art keywords
- seq
- polypeptide
- accordance
- aspergillus
- clostridium
- Prior art date
Links
- 101710112457 Exoglucanase Proteins 0.000 title claims 3
- 230000000694 effects Effects 0.000 title claims 3
- 229920001184 polypeptide Polymers 0.000 claims 7
- 102000004196 processed proteins & peptides Human genes 0.000 claims 7
- 108090000765 processed proteins & peptides Proteins 0.000 claims 7
- 239000002551 biofuel Substances 0.000 claims 5
- 238000000855 fermentation Methods 0.000 claims 5
- 230000004151 fermentation Effects 0.000 claims 5
- 102000004190 Enzymes Human genes 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 4
- 210000004027 cell Anatomy 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 238000002360 preparation method Methods 0.000 claims 4
- 239000002028 Biomass Substances 0.000 claims 3
- 241000193403 Clostridium Species 0.000 claims 3
- 229920002678 cellulose Polymers 0.000 claims 3
- 239000001913 cellulose Substances 0.000 claims 3
- 239000002029 lignocellulosic biomass Substances 0.000 claims 3
- 238000004519 manufacturing process Methods 0.000 claims 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 2
- 241000223198 Humicola Species 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 239000008346 aqueous phase Substances 0.000 claims 2
- 239000008103 glucose Substances 0.000 claims 2
- 238000000926 separation method Methods 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims 1
- 241000228212 Aspergillus Species 0.000 claims 1
- 241000351920 Aspergillus nidulans Species 0.000 claims 1
- 241000228245 Aspergillus niger Species 0.000 claims 1
- 240000006439 Aspergillus oryzae Species 0.000 claims 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims 1
- 241000228251 Aspergillus phoenicis Species 0.000 claims 1
- 241000228260 Aspergillus wentii Species 0.000 claims 1
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 229920002299 Cellodextrin Polymers 0.000 claims 1
- 241000186321 Cellulomonas Species 0.000 claims 1
- 241000123346 Chrysosporium Species 0.000 claims 1
- 241001674013 Chrysosporium lucknowense Species 0.000 claims 1
- 241000193401 Clostridium acetobutylicum Species 0.000 claims 1
- 101100373011 Drosophila melanogaster wapl gene Proteins 0.000 claims 1
- 241000588722 Escherichia Species 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 241000223218 Fusarium Species 0.000 claims 1
- 241000235058 Komagataella pastoris Species 0.000 claims 1
- 241000226677 Myceliophthora Species 0.000 claims 1
- 241000221960 Neurospora Species 0.000 claims 1
- 241000221961 Neurospora crassa Species 0.000 claims 1
- 241000228143 Penicillium Species 0.000 claims 1
- 241000985513 Penicillium oxalicum Species 0.000 claims 1
- 241000235648 Pichia Species 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 claims 1
- 241000235070 Saccharomyces Species 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 1
- 241000187747 Streptomyces Species 0.000 claims 1
- 241000606507 Talaromyces pinophilus Species 0.000 claims 1
- 241000203640 Thermomonospora Species 0.000 claims 1
- 241001313536 Thermothelomyces thermophila Species 0.000 claims 1
- 241000223259 Trichoderma Species 0.000 claims 1
- 241000378866 Trichoderma koningii Species 0.000 claims 1
- 241000499912 Trichoderma reesei Species 0.000 claims 1
- 241000223261 Trichoderma viride Species 0.000 claims 1
- 241000235013 Yarrowia Species 0.000 claims 1
- 241000235015 Yarrowia lipolytica Species 0.000 claims 1
- 241001656794 [Clostridium] saccharolyticum Species 0.000 claims 1
- 101150052795 cbh-1 gene Proteins 0.000 claims 1
- FYGDTMLNYKFZSV-ZWSAEMDYSA-N cellotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-ZWSAEMDYSA-N 0.000 claims 1
- 239000003593 chromogenic compound Substances 0.000 claims 1
- 230000007062 hydrolysis Effects 0.000 claims 1
- 238000006460 hydrolysis reaction Methods 0.000 claims 1
- 230000003301 hydrolyzing effect Effects 0.000 claims 1
- 208000012396 long COVID-19 Diseases 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 210000004483 pasc Anatomy 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Detergent Compositions (AREA)
Claims (13)
1. Izolirani ili pročišćeni polipeptid, naznačen time što ima aktivnost egzoglukanaze poboljšanu za najmanje 10 % na temperaturi od otprilike 35 °C, te izborno za najmanje 10 % na temperaturi od otprilike 50 °C, u odnosu na aktivnost egzoglukanaze referentnog proteina CBH1 sa slijedom SEQ ID NO: 2 na supstratu kojeg se bira između Avicel celuloze, PASC celuloze, reduciranog celodekstrinskog supstrata ili kromogenskog supstrata, poput pNP laktozida, gdje se navedeni polipeptid bira iz skupine koju čine:
i. aminokiselinski slijed kojeg se bira između SEQ ID NO: 8, SEQ ID NO: 4, SEQ ID NO: 16, SEQ ID NO: 6, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 18 i SEQ ID NO: 20, i
ii. aminokiselinski slijed koji ima, u odnosu na slijed SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 12, SEQ ID NO: 18 ili SEQ ID NO: 20, postotak istovjetnosti od najmanje 99 %.
2. Pročišćena ili izolirana nukleinska kiselina, naznačena time što kodira najmanje jedan polipeptid u skladu s patentnim zahtjevom 1.
3. Pročišćena ili izolirana nukleinska kiselina u skladu s patentnim zahtjevom 2, koju se bira između sljedećih sljedova: SEQ ID NO: 7, SEQ ID NO: 3, SEQ ID NO: 15, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 17 i SEQ ID NO: 19.
4. Vektor, naznačen time što sadrži najmanje jednu nukleinsku kiselinu u skladu s bilo kojim od patentnih zahtjeva 2 ili 3.
5. Izolirana stanica domaćin, naznačena time što sadrži najmanje jedan polipeptid u skladu s patentnim zahtjevom 1, ili najmanje jednu nukleinsku kiselinu u skladu s bilo kojim od patentnih zahtjeva 2 ili 3, ili najmanje jedan vektor u skladu s patentnim zahtjevom 4.
6. Izolirana stanica domaćin u skladu s patentnim zahtjevom 5, naznačena time što je se bira između rodova Trichoderma, Aspergillus, Neurospora, Humicola, Penicillium, Fusarium, Thermomonospora, Myceliophthora, Chrysosporium, Bacillus, Pseudomonas, Escherichia, Clostridium, Cellulomonas, Streptomyces, Yarrowia, Pichia i Saccharomyces.
7. Izolirana stanica domaćin u skladu s bilo kojim od patentnih zahtjeva 5 ili 6, naznačena time što je se bira između vrsta Trichoderma reesei, Trichoderma viridae, Trichoderma koningii, Aspergillus niger, Aspergillus nidulans, Aspergillus wentii, Aspergillus oryzae, Aspergillus phoenicis, Neurospora crassa, Humicola grisae, Myceliophthora thermophila, Chrysosporium lucknowense, Penicillium pinophilum, Penicillium oxalicum, Escherichia coli, Clostridium acetobutylicum, Clostridium saccharolyticum, Clostridium benjerinckii, Clostridium butylicum, Pichia pastoris, Yarrowia lipolityca i Saccharomyces cerevisiae.
8. Upotreba navedenog polipeptida u skladu s patentnim zahtjevom 1 u hidrolizi celuloze.
9. Upotreba navedenog polipeptida u skladu s patentnim zahtjevom 1 u proizvodnji biogoriva.
10. Enzimski pripravak koji može djelovati na lignoceluloznu biomasu, gdje navedeni enzimski pripravak proizvode filamentozne gljivice i sadrži najmanje jedan polipeptid u skladu s patentnim zahtjevom 1.
11. Postupak proizvodnje biogoriva iz lignocelulozne biomase, naznačen time što se sastoji u sljedećim uzastopnim koracima:
– suspendiranju biomase koju treba hidrolizirati u vodenoj fazi;
– hidroliziranju lignocelulozne biomase u prisutnosti enzimskog pripravka u skladu s patentnim zahtjevom 10 kako bi se proizveo hidrolizat koji sadrži glukozu;
– fermentiranju glukoze iz hidrolizata kako bi se proizvela fermentacijska masa;
– odvajanju biogoriva iz fermentacijske mase.
12. Postupak proizvodnje biogoriva iz biomase, naznačen time što se sastoji u sljedećim uzastopnim koracima:
– suspendiranju biomase koju treba hidrolizirati u vodenoj fazi;
– istodobnom dodavanju enzimskog pripravka u skladu s patentnim zahtjevom 10 i fermentirajućeg organizma u suspenziju i fermentiranju smjese kako bi se proizvela fermentacijska masa;
– odvajanju biogoriva iz fermentacijske mase.
13. Postupak u skladu s bilo kojim od patentnih zahtjeva 11 ili 12, gdje je fermentirajući organizam stanica domaćin u skladu s bilo kojim od patentnih zahtjeva 5 do 7.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1455700A FR3022557B1 (fr) | 2014-06-20 | 2014-06-20 | Variants d'exoglucanases a activite amelioree et leurs utilisations |
| EP15732868.3A EP3158071B1 (fr) | 2014-06-20 | 2015-06-12 | Variants d'exoglucanases a activite amelioree et leurs utilisations |
| PCT/FR2015/051556 WO2015193587A1 (fr) | 2014-06-20 | 2015-06-12 | Variants d'exoglucanases a activite amelioree et leurs utilisations |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20211237T1 true HRP20211237T1 (hr) | 2022-01-07 |
Family
ID=51570606
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20211237TT HRP20211237T1 (hr) | 2014-06-20 | 2015-06-12 | Varijante egzoglukanaza s poboljšanom aktivnošću i njihova upotreba |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US10745679B2 (hr) |
| EP (1) | EP3158071B1 (hr) |
| CN (1) | CN106459942B (hr) |
| BR (1) | BR112016029572B1 (hr) |
| CA (1) | CA2950077C (hr) |
| DK (1) | DK3158071T3 (hr) |
| ES (1) | ES2876268T3 (hr) |
| FR (2) | FR3022557B1 (hr) |
| HR (1) | HRP20211237T1 (hr) |
| HU (1) | HUE055330T2 (hr) |
| PL (1) | PL3158071T3 (hr) |
| PT (1) | PT3158071T (hr) |
| WO (1) | WO2015193587A1 (hr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109072209A (zh) * | 2016-03-24 | 2018-12-21 | 诺维信公司 | 纤维二糖水解酶变体和编码其的多核苷酸 |
| CN107988127B (zh) * | 2017-11-02 | 2021-09-03 | 南京农业大学 | 里氏木霉木质纤维素酶基因工程乳酸菌组合在调制优质苜蓿青贮饲料中的应用 |
| EP4055177B1 (en) * | 2019-11-08 | 2024-07-31 | Danisco US Inc. | Fungal strains comprising enhanced protein productivity phenotypes and methods thereof |
| FR3113291A1 (fr) | 2020-08-06 | 2022-02-11 | IFP Energies Nouvelles | Procédé de production d’alcool par hydrolyse enzymatique et fermentation de biomasse lignocellulosique |
| FR3134103B1 (fr) | 2022-03-30 | 2024-12-20 | Ifp Energies Now | Procédé de production d’enzymes cellulolytiques et/ou hémicellulytiques |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2782323B1 (fr) | 1998-08-12 | 2002-01-11 | Proteus | Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues |
| US6951719B1 (en) | 1999-08-11 | 2005-10-04 | Proteus S.A. | Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained |
| US8637293B2 (en) * | 1999-07-13 | 2014-01-28 | Alliance For Sustainable Energy, Llc | Cellobiohydrolase I enzymes |
| US7972832B2 (en) * | 2002-08-16 | 2011-07-05 | Danisco Us Inc. | Variant Hypocrea jecorina CBH1 cellulases |
| ES2538824T3 (es) * | 2003-08-25 | 2015-06-24 | Novozymes, Inc. | Variantes de glucósido hidrolasas |
| FR2871170B1 (fr) | 2004-06-07 | 2006-08-11 | Proteus Sa | Procede de determination de la charge mutationnelle d'une banque de genes obtenue par mutagenese aleatoire d'un gene d'interet et les moyens pour sa mise en oeuvre |
| EP1999257B1 (en) | 2006-03-20 | 2011-06-22 | Novozymes, Inc. | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| AU2009287325A1 (en) | 2008-08-29 | 2010-03-04 | Iogen Energy Corporation | Modified beta-glucosidases with improved stability |
| FR2935986B1 (fr) | 2008-09-12 | 2012-11-23 | Inst Francais Du Petrole | Variants de beta-glucosidase a activite amelioree et leurs utilisations. |
| WO2010066411A2 (en) | 2008-12-10 | 2010-06-17 | Direvo Industrial Biotechnology Gmbh | Polypeptides having cellobiohydrolase ii activity |
| FI122029B (fi) | 2008-12-30 | 2011-07-29 | Ab Enzymes Oy | Sieniperäiset endoglukanaasit, niiden tuotto ja käyttö |
| US20100304464A1 (en) | 2009-04-06 | 2010-12-02 | California Institute Of Technology | Stable, functional chimeric cellobiohydrolases |
| CA2763836A1 (en) | 2009-05-29 | 2010-12-02 | Iogen Energy Corporation | Novel beta-glucosidase enzymes |
| WO2011050037A1 (en) * | 2009-10-23 | 2011-04-28 | Novozymes, Inc. | Cellobiohydrolase variants and polynucleotides encoding same |
| WO2012068509A1 (en) * | 2010-11-18 | 2012-05-24 | Novozymes, Inc. | Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| WO2012135659A2 (en) | 2011-03-31 | 2012-10-04 | Novozymes A/S | Methods for enhancing the degradation or conversion of cellulosic material |
| DK2702153T3 (en) | 2011-04-28 | 2019-03-18 | Novozymes Inc | Polypeptides with endoglucanase activity and polynucleotides encoding them |
| DK2702162T3 (da) | 2011-04-29 | 2020-05-18 | Novozymes Inc | Fremgangsmåder til forbedring af nedbrydningen eller omdannelsen af celluloseholdigt materiale |
| US9279163B2 (en) | 2011-08-31 | 2016-03-08 | Iogen Energy Corporation | Cellobiohydrolase enzymes |
| FR2989385B1 (fr) | 2012-04-13 | 2014-05-23 | IFP Energies Nouvelles | Procede de selection de clones de souches microbiennes ou de cellules animales, notamment pour la production de proteines |
| CN104870642A (zh) * | 2012-12-12 | 2015-08-26 | 丹尼斯科美国公司 | 纤维二糖水解酶的变体 |
| BR112015013646A2 (pt) * | 2012-12-12 | 2017-11-14 | Danisco Us Inc | variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo cbh variante e sobrenadante de cultura celular |
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2014
- 2014-06-20 FR FR1455700A patent/FR3022557B1/fr active Active
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2015
- 2015-06-12 BR BR112016029572-2A patent/BR112016029572B1/pt active IP Right Grant
- 2015-06-12 EP EP15732868.3A patent/EP3158071B1/fr active Active
- 2015-06-12 CA CA2950077A patent/CA2950077C/fr active Active
- 2015-06-12 PL PL15732868T patent/PL3158071T3/pl unknown
- 2015-06-12 ES ES15732868T patent/ES2876268T3/es active Active
- 2015-06-12 WO PCT/FR2015/051556 patent/WO2015193587A1/fr not_active Ceased
- 2015-06-12 US US15/320,425 patent/US10745679B2/en active Active
- 2015-06-12 HR HRP20211237TT patent/HRP20211237T1/hr unknown
- 2015-06-12 HU HUE15732868A patent/HUE055330T2/hu unknown
- 2015-06-12 CN CN201580032312.3A patent/CN106459942B/zh active Active
- 2015-06-12 PT PT157328683T patent/PT3158071T/pt unknown
- 2015-06-12 DK DK15732868.3T patent/DK3158071T3/da active
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2018
- 2018-11-09 FR FR1860390A patent/FR3073230A1/fr active Pending
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2020
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Also Published As
| Publication number | Publication date |
|---|---|
| DK3158071T3 (da) | 2021-08-16 |
| PL3158071T3 (pl) | 2022-01-03 |
| US11286472B2 (en) | 2022-03-29 |
| FR3022557A1 (fr) | 2015-12-25 |
| CA2950077C (fr) | 2023-07-18 |
| CN106459942B (zh) | 2020-04-03 |
| PT3158071T (pt) | 2021-06-28 |
| BR112016029572A2 (pt) | 2017-10-24 |
| US20170152497A1 (en) | 2017-06-01 |
| BR112016029572B1 (pt) | 2023-11-07 |
| FR3022557B1 (fr) | 2019-01-25 |
| CA2950077A1 (fr) | 2015-12-23 |
| ES2876268T3 (es) | 2021-11-12 |
| HUE055330T2 (hu) | 2021-11-29 |
| US20200347371A1 (en) | 2020-11-05 |
| US10745679B2 (en) | 2020-08-18 |
| FR3073230A1 (fr) | 2019-05-10 |
| CN106459942A (zh) | 2017-02-22 |
| EP3158071B1 (fr) | 2021-05-19 |
| EP3158071A1 (fr) | 2017-04-26 |
| WO2015193587A1 (fr) | 2015-12-23 |
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