HUT73739A - Integrative dna segment compraising gene encoding insecticidal protein - Google Patents
Integrative dna segment compraising gene encoding insecticidal protein Download PDFInfo
- Publication number
- HUT73739A HUT73739A HU9503017A HU9503017A HUT73739A HU T73739 A HUT73739 A HU T73739A HU 9503017 A HU9503017 A HU 9503017A HU 9503017 A HU9503017 A HU 9503017A HU T73739 A HUT73739 A HU T73739A
- Authority
- HU
- Hungary
- Prior art keywords
- dna
- plasmid
- gene
- cells
- sequence
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims description 259
- 230000000749 insecticidal effect Effects 0.000 title claims description 99
- 102000004169 proteins and genes Human genes 0.000 title claims description 89
- 108020004414 DNA Proteins 0.000 claims description 164
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 88
- 238000000034 method Methods 0.000 claims description 65
- 239000013598 vector Substances 0.000 claims description 50
- 239000000203 mixture Substances 0.000 claims description 32
- 239000003550 marker Substances 0.000 claims description 29
- 239000013611 chromosomal DNA Substances 0.000 claims description 28
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 27
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 25
- 230000010076 replication Effects 0.000 claims description 23
- 241000894006 Bacteria Species 0.000 claims description 19
- 230000001580 bacterial effect Effects 0.000 claims description 18
- 108091026890 Coding region Proteins 0.000 claims description 17
- 238000010367 cloning Methods 0.000 claims description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 7
- 238000003776 cleavage reaction Methods 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 7
- 230000007017 scission Effects 0.000 claims description 7
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 230000002463 transducing effect Effects 0.000 claims description 3
- 241001147758 Bacillus thuringiensis serovar kurstaki Species 0.000 claims description 2
- 108020005091 Replication Origin Proteins 0.000 claims description 2
- 108020004638 Circular DNA Proteins 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 230000008775 paternal effect Effects 0.000 claims 1
- 239000013612 plasmid Substances 0.000 description 236
- 210000004027 cell Anatomy 0.000 description 169
- 239000012634 fragment Substances 0.000 description 94
- 235000018102 proteins Nutrition 0.000 description 76
- 210000000349 chromosome Anatomy 0.000 description 36
- 108020004707 nucleic acids Proteins 0.000 description 35
- 102000039446 nucleic acids Human genes 0.000 description 35
- 150000007523 nucleic acids Chemical class 0.000 description 35
- 239000002609 medium Substances 0.000 description 34
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 32
- 230000010354 integration Effects 0.000 description 31
- 239000004098 Tetracycline Substances 0.000 description 27
- 229960002180 tetracycline Drugs 0.000 description 27
- 229930101283 tetracycline Natural products 0.000 description 27
- 235000019364 tetracycline Nutrition 0.000 description 27
- 150000003522 tetracyclines Chemical class 0.000 description 27
- 108091034117 Oligonucleotide Proteins 0.000 description 24
- 241000588724 Escherichia coli Species 0.000 description 22
- 239000000523 sample Substances 0.000 description 21
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 20
- 239000013078 crystal Substances 0.000 description 19
- 238000004520 electroporation Methods 0.000 description 17
- 229960003276 erythromycin Drugs 0.000 description 16
- 108010017826 DNA Polymerase I Proteins 0.000 description 15
- 102000004594 DNA Polymerase I Human genes 0.000 description 15
- 101150016744 ermC gene Proteins 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 15
- 238000010361 transduction Methods 0.000 description 15
- 230000017105 transposition Effects 0.000 description 15
- 230000026683 transduction Effects 0.000 description 14
- 230000009466 transformation Effects 0.000 description 14
- 241000196324 Embryophyta Species 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 11
- 238000010276 construction Methods 0.000 description 11
- 238000010790 dilution Methods 0.000 description 11
- 239000012895 dilution Substances 0.000 description 11
- 239000002917 insecticide Substances 0.000 description 11
- 101710151559 Crystal protein Proteins 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 241000238631 Hexapoda Species 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 230000029087 digestion Effects 0.000 description 10
- 238000009396 hybridization Methods 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 238000001228 spectrum Methods 0.000 description 10
- 239000000872 buffer Substances 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 230000002759 chromosomal effect Effects 0.000 description 9
- 230000006801 homologous recombination Effects 0.000 description 9
- 238000002744 homologous recombination Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 229920001817 Agar Polymers 0.000 description 8
- 241000193755 Bacillus cereus Species 0.000 description 8
- 239000008272 agar Substances 0.000 description 8
- 239000000499 gel Substances 0.000 description 8
- 239000006166 lysate Substances 0.000 description 8
- 244000005700 microbiome Species 0.000 description 8
- 230000001105 regulatory effect Effects 0.000 description 8
- 230000002588 toxic effect Effects 0.000 description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 7
- 231100000331 toxic Toxicity 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 229960000723 ampicillin Drugs 0.000 description 6
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 6
- 238000004166 bioassay Methods 0.000 description 6
- 101150044717 cyt1Aa gene Proteins 0.000 description 6
- 101150040669 petJ gene Proteins 0.000 description 6
- 108091008146 restriction endonucleases Proteins 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 230000028070 sporulation Effects 0.000 description 6
- 239000005720 sucrose Substances 0.000 description 6
- 238000013519 translation Methods 0.000 description 6
- 241000192125 Firmicutes Species 0.000 description 5
- 101000896726 Homo sapiens Lanosterol 14-alpha demethylase Proteins 0.000 description 5
- 102100021695 Lanosterol 14-alpha demethylase Human genes 0.000 description 5
- 238000010222 PCR analysis Methods 0.000 description 5
- 229930006000 Sucrose Natural products 0.000 description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
- 108010079194 Type C Phospholipases Proteins 0.000 description 5
- 230000003115 biocidal effect Effects 0.000 description 5
- 239000000969 carrier Substances 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 230000001332 colony forming effect Effects 0.000 description 5
- 230000004927 fusion Effects 0.000 description 5
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000013605 shuttle vector Substances 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 108020004705 Codon Proteins 0.000 description 4
- 108091029865 Exogenous DNA Proteins 0.000 description 4
- 235000019733 Fish meal Nutrition 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 241000256247 Spodoptera exigua Species 0.000 description 4
- 101150078565 TEF gene Proteins 0.000 description 4
- 102000014384 Type C Phospholipases Human genes 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 150000007513 acids Chemical class 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 229960005091 chloramphenicol Drugs 0.000 description 4
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 4
- 239000006792 cys medium Substances 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 4
- 239000004467 fishmeal Substances 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 101150066555 lacZ gene Proteins 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 238000002944 PCR assay Methods 0.000 description 3
- 108010006785 Taq Polymerase Proteins 0.000 description 3
- 239000007984 Tris EDTA buffer Substances 0.000 description 3
- 230000002411 adverse Effects 0.000 description 3
- 239000011543 agarose gel Substances 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000001976 enzyme digestion Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- -1 for example Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 238000010348 incorporation Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000001418 larval effect Effects 0.000 description 3
- 239000013600 plasmid vector Substances 0.000 description 3
- 210000001938 protoplast Anatomy 0.000 description 3
- 230000003362 replicative effect Effects 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 230000001131 transforming effect Effects 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 108700003918 Bacillus Thuringiensis insecticidal crystal Proteins 0.000 description 2
- 241001071944 Cyta Species 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 108010042653 IgA receptor Proteins 0.000 description 2
- 206010061217 Infestation Diseases 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 2
- 241000255777 Lepidoptera Species 0.000 description 2
- 239000006142 Luria-Bertani Agar Substances 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 102100034014 Prolyl 3-hydroxylase 3 Human genes 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 239000012160 loading buffer Substances 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 238000013492 plasmid preparation Methods 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000006152 selective media Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000009261 transgenic effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
- 108020005065 3' Flanking Region Proteins 0.000 description 1
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
- 108020005029 5' Flanking Region Proteins 0.000 description 1
- 241000238876 Acari Species 0.000 description 1
- 241000023308 Acca Species 0.000 description 1
- 241000589220 Acetobacter Species 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 241000256118 Aedes aegypti Species 0.000 description 1
- 241000607534 Aeromonas Species 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 101710171801 Alpha-amylase inhibitor Proteins 0.000 description 1
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 241000589151 Azotobacter Species 0.000 description 1
- 241000304886 Bacilli Species 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 101100219198 Bacillus thuringiensis subsp. israelensis cry11Aa gene Proteins 0.000 description 1
- 231100000699 Bacterial toxin Toxicity 0.000 description 1
- 241000588807 Bordetella Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000589562 Brucella Species 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 241000256113 Culicidae Species 0.000 description 1
- 238000007900 DNA-DNA hybridization Methods 0.000 description 1
- 241000605716 Desulfovibrio Species 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000507581 Ergene Species 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 1
- 102100030771 Ferrochelatase, mitochondrial Human genes 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 241000256244 Heliothis virescens Species 0.000 description 1
- 101000843611 Homo sapiens Ferrochelatase, mitochondrial Proteins 0.000 description 1
- 241000721703 Lymantria dispar Species 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 description 1
- 241000588653 Neisseria Species 0.000 description 1
- 241000605159 Nitrobacter Species 0.000 description 1
- 241000605122 Nitrosomonas Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 206010029897 Obsessive thoughts Diseases 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 241000193418 Paenibacillus larvae Species 0.000 description 1
- 241000606860 Pasteurella Species 0.000 description 1
- 208000037581 Persistent Infection Diseases 0.000 description 1
- 108010044302 Phosphoinositide phospholipase C Proteins 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 241000607568 Photobacterium Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241000605008 Spirillum Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000008051 TBE buffer Substances 0.000 description 1
- 239000007994 TES buffer Substances 0.000 description 1
- 241000605118 Thiobacillus Species 0.000 description 1
- 108010001244 Tli polymerase Proteins 0.000 description 1
- 241000255993 Trichoplusia ni Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241001148134 Veillonella Species 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 241000588901 Zymomonas Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 239000003392 amylase inhibitor Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229940065181 bacillus anthracis Drugs 0.000 description 1
- 210000003578 bacterial chromosome Anatomy 0.000 description 1
- 239000000688 bacterial toxin Substances 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 239000007478 blood agar base Substances 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 210000004900 c-terminal fragment Anatomy 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- 108010079058 casein hydrolysate Proteins 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 239000013256 coordination polymer Substances 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000009585 enzyme analysis Methods 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 150000002484 inorganic compounds Chemical class 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- 229960002216 methylparaben Drugs 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 238000007500 overflow downdraw method Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 101150093672 pcl gene Proteins 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000003016 pheromone Substances 0.000 description 1
- 229940095591 phoslo Drugs 0.000 description 1
- 108010002205 phospholipase C1 Proteins 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920001083 polybutene Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 239000011833 salt mixture Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 125000000446 sulfanediyl group Chemical group *S* 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
- 235000009529 zinc sulphate Nutrition 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5279093A | 1993-04-23 | 1993-04-23 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| HU9503017D0 HU9503017D0 (en) | 1995-12-28 |
| HUT73739A true HUT73739A (en) | 1996-09-30 |
Family
ID=21979908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU9503017A HUT73739A (en) | 1993-04-23 | 1994-04-21 | Integrative dna segment compraising gene encoding insecticidal protein |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP0696324A1 (pt) |
| JP (1) | JPH08509608A (pt) |
| KR (1) | KR960702001A (pt) |
| CN (1) | CN1121733A (pt) |
| AU (1) | AU685516B2 (pt) |
| BR (1) | BR9406536A (pt) |
| CA (1) | CA2159323A1 (pt) |
| CZ (1) | CZ275195A3 (pt) |
| HU (1) | HUT73739A (pt) |
| IL (1) | IL109367A0 (pt) |
| PL (1) | PL311205A1 (pt) |
| WO (1) | WO1994025611A1 (pt) |
| ZA (1) | ZA942824B (pt) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6280721B1 (en) | 1989-12-18 | 2001-08-28 | Valent Biosciences, Inc. | Production of Bacillus thuringiensis integrants |
| US6270760B1 (en) | 1989-12-18 | 2001-08-07 | Valent Biosciences, Inc. | Production of Bacillus thuringiensis integrants |
| IL110299A0 (en) | 1993-07-15 | 1994-10-21 | Novo Nordisk Entotech Inc | Formation of and methods for the production of large bacillus thuringiensis crystals with increased pesticidal activity |
| EP0876493B1 (en) * | 1996-01-26 | 2004-04-14 | Valent Biosciences, Corp. | Production of bacillus thuringiensis integrants |
| US5804180A (en) * | 1996-07-17 | 1998-09-08 | Ecogen, Inc. | Bacillus thuringiensis strains showing improved production of certain lepidopteran-toxic crystal proteins |
| CN103205392A (zh) * | 2013-04-17 | 2013-07-17 | 江苏里下河地区农业科学研究所 | 一种染色体重组增效蛋白基因苏云金杆菌工程菌及其构建方法 |
| NL2022581B1 (en) * | 2019-02-14 | 2020-08-27 | Koppert Bv | Composition comprising a mixture of dna molecules, uses thereof as biological inhibitor and method for production |
| CN111793637B (zh) * | 2020-07-24 | 2023-04-14 | 海口海森元生物科技有限公司 | 一种细菌磷脂酰肌醇特异性磷脂酶c基因及其应用 |
| AR134711A1 (es) * | 2023-12-18 | 2026-03-04 | Syngenta Crop Protection Ag | Formulación |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1341092C (en) * | 1985-12-12 | 2000-09-05 | David L. Edwards | Process for altering the host range of bacillus thuringiensis toxins, and novel toxins produced thereby |
| EP0342633B1 (de) * | 1988-05-20 | 1997-01-08 | Ciba-Geigy Ag | Bacillus thuringiensis Transformation |
| WO1993003619A1 (en) * | 1991-08-19 | 1993-03-04 | Research Corporation Technologies, Inc. | Multi-targeted bacillus thuringiensis bioinsecticide |
-
1994
- 1994-04-21 WO PCT/EP1994/001249 patent/WO1994025611A1/en not_active Ceased
- 1994-04-21 EP EP94915528A patent/EP0696324A1/en not_active Ceased
- 1994-04-21 AU AU78796/94A patent/AU685516B2/en not_active Ceased
- 1994-04-21 CZ CZ952751A patent/CZ275195A3/cs unknown
- 1994-04-21 KR KR1019950704599A patent/KR960702001A/ko not_active Ceased
- 1994-04-21 JP JP6523840A patent/JPH08509608A/ja not_active Ceased
- 1994-04-21 PL PL94311205A patent/PL311205A1/xx unknown
- 1994-04-21 HU HU9503017A patent/HUT73739A/hu unknown
- 1994-04-21 CN CN94191880A patent/CN1121733A/zh active Pending
- 1994-04-21 IL IL10936794A patent/IL109367A0/xx unknown
- 1994-04-21 BR BR9406536A patent/BR9406536A/pt not_active Application Discontinuation
- 1994-04-21 CA CA002159323A patent/CA2159323A1/en not_active Abandoned
- 1994-04-22 ZA ZA942824A patent/ZA942824B/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CZ275195A3 (en) | 1996-01-17 |
| KR960702001A (ko) | 1996-03-28 |
| PL311205A1 (en) | 1996-02-05 |
| ZA942824B (en) | 1995-10-23 |
| HU9503017D0 (en) | 1995-12-28 |
| AU7879694A (en) | 1994-11-21 |
| IL109367A0 (en) | 1994-07-31 |
| CA2159323A1 (en) | 1994-11-10 |
| JPH08509608A (ja) | 1996-10-15 |
| BR9406536A (pt) | 1996-01-02 |
| CN1121733A (zh) | 1996-05-01 |
| WO1994025611A1 (en) | 1994-11-10 |
| EP0696324A1 (en) | 1996-02-14 |
| AU685516B2 (en) | 1998-01-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5441884A (en) | Bacillus thuringiensis transposon TN5401 | |
| RU2106409C1 (ru) | Выделенный и очищенный фрагмент днк cry iiic, токсичный для жесткокрылых белок cry iiic, инсектицидная композиция для борьбы с жесткокрылыми насекомыми (варианты), штамм бактерий bacillus thuringiensis - продуцент токсичного для жесткокрылых белка cry iiic (варианты), токсичный для жесткокрылых белок, способ борьбы с жесткокрылыми насекомыми | |
| JP4647099B2 (ja) | 約15kDaおよび約45kDaの殺虫性タンパク質をコードする、植物において最も効果的に発現するポリヌクレオチド | |
| JP4382277B2 (ja) | 殺虫性毒素をコードする植物最適化遺伝子 | |
| Barloy et al. | Cloning and expression of the first anaerobic toxin gene from Clostridium bifermentans subsp. malaysia, encoding a new mosquitocidal protein with homologies to Bacillus thuringiensis delta-endotoxins | |
| US5776449A (en) | Recombinant bacillus thuringiensis strains, insecticidal compositions and method of use | |
| Poncet et al. | Improvement of Bacillus sphaericus toxicity against dipteran larvae by integration, via homologous recombination, of the Cry11A toxin gene from Bacillus thuringiensis subsp. israelensis | |
| Sanchis et al. | Construction of new insecticidal Bacillus thuringiensis recombinant strains by using the sporulation non-dependent expression system of cryIIIA and a site specific recombination vector | |
| Sanchis et al. | A recombinase-mediated system for elimination of antibiotic resistance gene markers from genetically engineered Bacillus thuringiensis strains | |
| AU647121B2 (en) | Bacillus thuringiensis cryIF and cryIX genes and proteins toxic to lepidopteran insects | |
| HUT73739A (en) | Integrative dna segment compraising gene encoding insecticidal protein | |
| US6096306A (en) | Strains of Bacillus thuringiensis and pesticide composition containing them | |
| JP2531913B2 (ja) | バシルス チュリンギエンシス(Basillus thuringiensis) cryIIIC(b)毒素遺伝子及び甲虫類昆虫に毒性のタンパク質 | |
| AU643800B2 (en) | Shuttle vector for recombinant bacillus thuringiensis strain development | |
| KR100358269B1 (ko) | 증가된살충활성을지닌거대한바실러스투린지엔시스결정의형성및이의제조방법 | |
| Chak et al. | Cloning and expression in Escherichia coli of an insecticidal crystal protein gene from Bacillus thuringiensis var. aizawai HD-133 | |
| Park et al. | Genetic engineering of bacteria to improve efficacy using the insecticidal proteins of Bacillus species. | |
| US11375720B2 (en) | Construction of a quadruple enterotoxin-deficient mutant of Bacillus thuringiensis | |
| Yue et al. | Broadening the insecticidal spectrum of Lepidoptera‐specific Bacillus thuringiensis strains by chromosomal integration of cry3A | |
| Roh et al. | Expression and characterization of a recombinant Cry1Ac crystal protein with enhanced green fluorescent protein in acrystalliferous Bacillus thuringiensis | |
| US6482636B1 (en) | Constructed Bacillus thuringiensis strains producing mosquitocidal crystal proteins | |
| Kelly | Optimized electroporation conditions for Bacillus sphaericus 2362 | |
| PARK et al. | 12 Genetic Engineering of |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DGB9 | Succession in title of applicant |
Owner name: NOVARTIS AG., CH |
|
| DFD9 | Temporary protection cancelled due to non-payment of fee |