IE32962L - Penicillin acylase composition - Google Patents
Penicillin acylase compositionInfo
- Publication number
- IE32962L IE32962L IE690199A IE19969A IE32962L IE 32962 L IE32962 L IE 32962L IE 690199 A IE690199 A IE 690199A IE 19969 A IE19969 A IE 19969A IE 32962 L IE32962 L IE 32962L
- Authority
- IE
- Ireland
- Prior art keywords
- enzyme
- cell
- cell material
- esp
- chains
- Prior art date
Links
- 108010073038 Penicillin Amidase Proteins 0.000 title abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 6
- 102000004190 Enzymes Human genes 0.000 abstract 6
- 239000000463 material Substances 0.000 abstract 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 abstract 2
- NGHVIOIJCVXTGV-ALEPSDHESA-N 6-aminopenicillanic acid Chemical compound [O-]C(=O)[C@H]1C(C)(C)S[C@@H]2[C@H]([NH3+])C(=O)N21 NGHVIOIJCVXTGV-ALEPSDHESA-N 0.000 abstract 1
- NGHVIOIJCVXTGV-UHFFFAOYSA-N 6beta-amino-penicillanic acid Natural products OC(=O)C1C(C)(C)SC2C(N)C(=O)N21 NGHVIOIJCVXTGV-UHFFFAOYSA-N 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 229930182555 Penicillin Natural products 0.000 abstract 1
- 238000007385 chemical modification Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000003834 intracellular effect Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 235000019371 penicillin G benzathine Nutrition 0.000 abstract 1
- 229940056360 penicillin g Drugs 0.000 abstract 1
- 150000002960 penicillins Chemical class 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D499/00—Heterocyclic compounds containing 4-thia-1-azabicyclo [3.2.0] heptane ring systems, i.e. compounds containing a ring system of the formula:, e.g. penicillins, penems; Such ring systems being further condensed, e.g. 2,3-condensed with an oxygen-, nitrogen- or sulfur-containing hetero ring
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/84—Penicillin amidase (3.5.1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
- C12P37/04—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by acylation of the substituent in the 6 position
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
- C12P37/06—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by desacylation of the substituent in the 6 position
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Handcart (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Cleaning Implements For Floors, Carpets, Furniture, Walls, And The Like (AREA)
Abstract
Prepn. of cell-free penicillin-acylase by fermenting a strain of E. coli which produces this enzyme. The intracellular enzyme is separated from the culture liquid at controlled temps. between 0-50 deg.C by fast removal of the cell material through a slot or opening at a pressure of at least 500 psi but below the pressure which would badly tear the cells. The removed cell material is then stirred in water for 0.1-5 hrs. at 10-50 deg.C to dissolve the enzyme; this is extracted from the inactive cell material in aqs. soln. The cell-free enzymes are used for the prodn. of 6-APA and are esp. suitable for direct removal of side-chains of natural penicillins, esp. the side-chains of benzyl penicillin. The enzymes may be used as starting materials for chemical modification of the enzyme, giving products with higher activity and/or wider technical application.
[FR2001977A1]
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB754468 | 1968-02-15 | ||
| GB57373/68A GB1261711A (en) | 1968-02-15 | 1968-12-03 | Process for producing a penicillin acylose composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE32962L true IE32962L (en) | 1969-08-15 |
| IE32962B1 IE32962B1 (en) | 1974-02-06 |
Family
ID=26241501
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE199/69A IE32962B1 (en) | 1968-02-15 | 1969-02-14 | Process for producing a penicillin acylose composition |
Country Status (17)
| Country | Link |
|---|---|
| JP (2) | JPS4833386B1 (en) |
| AT (2) | AT300198B (en) |
| BE (1) | BE727506A (en) |
| CA (1) | CA941769A (en) |
| CH (2) | CH523320A (en) |
| CS (1) | CS155970B2 (en) |
| DE (4) | DE1966427C3 (en) |
| DK (1) | DK128421B (en) |
| ES (4) | ES363592A1 (en) |
| FI (1) | FI47109C (en) |
| FR (2) | FR2001977A1 (en) |
| IE (1) | IE32962B1 (en) |
| IT (1) | IT1061759B (en) |
| NL (1) | NL158547B (en) |
| NO (3) | NO131388C (en) |
| SE (3) | SE382990B (en) |
| SU (1) | SU404195A3 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1357317A (en) * | 1970-08-27 | 1974-06-19 | Beecham Group Ltd | Enzymes |
| IL39158A (en) * | 1971-04-28 | 1977-08-31 | Snam Progetti | Enzymatic scission and synthesis of penicillins and cephalosporins |
| BE791748A (en) * | 1971-11-23 | 1973-05-22 | Bayer Ag | PROCESS FOR THE PRODUCTION OF 6-AMINOPENICILLANIC ACID BY ENZYMATIC RUPTURE OF PENICILLINS |
| JPS5420193A (en) * | 1977-07-12 | 1979-02-15 | Tanabe Seiyaku Co Ltd | Immobilized aminoacylase and its preparation |
| GB9718740D0 (en) * | 1997-09-05 | 1997-11-12 | Advanced Phytonics Ltd | Improvements in or relating to the preparation of a compound |
| DE10123048A1 (en) * | 2001-05-11 | 2002-11-21 | Bermatingen Maschf | Cutter for rotary mower and mulcher comprises central mounting attached to drive shaft, cutter blades being attached to sloping mounting arms which are bolted to underside of central mounting |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB877898A (en) * | 1956-12-06 | 1961-09-20 | Apv Co Ltd | A new or improved method of liberating enzymes |
| GB1118566A (en) * | 1964-11-25 | 1968-07-03 | Beecham Group Ltd | Production of 6-aminopenicillanic acid |
-
1969
- 1969-01-24 BE BE727506D patent/BE727506A/xx unknown
- 1969-02-01 IT IT34651/69A patent/IT1061759B/en active
- 1969-02-04 CA CA041,889A patent/CA941769A/en not_active Expired
- 1969-02-06 AT AT100971A patent/AT300198B/en not_active IP Right Cessation
- 1969-02-06 AT AT118469A patent/AT297218B/en not_active IP Right Cessation
- 1969-02-10 NL NL6902045.A patent/NL158547B/en not_active IP Right Cessation
- 1969-02-11 SE SE7111628A patent/SE382990B/en unknown
- 1969-02-11 SE SE01820/69A patent/SE352643B/xx unknown
- 1969-02-13 ES ES363592A patent/ES363592A1/en not_active Expired
- 1969-02-13 SU SU1309423A patent/SU404195A3/ru active
- 1969-02-14 DK DK80469AA patent/DK128421B/en unknown
- 1969-02-14 DE DE1966427A patent/DE1966427C3/en not_active Expired
- 1969-02-14 CS CS176071*1A patent/CS155970B2/cs unknown
- 1969-02-14 DE DE19691907365 patent/DE1907365A1/en active Pending
- 1969-02-14 DE DE1966428A patent/DE1966428C3/en not_active Expired
- 1969-02-14 FR FR6903732A patent/FR2001977A1/en active Granted
- 1969-02-14 IE IE199/69A patent/IE32962B1/en unknown
- 1969-02-14 FI FI690482A patent/FI47109C/en active
- 1969-02-14 CH CH226769A patent/CH523320A/en not_active IP Right Cessation
- 1969-02-14 NO NO610/69A patent/NO131388C/no unknown
- 1969-02-14 DE DE19691933301 patent/DE1933301A1/en active Pending
- 1969-02-15 JP JP44010799A patent/JPS4833386B1/ja active Pending
- 1969-05-23 NO NO692139A patent/NO131387C/no unknown
-
1970
- 1970-09-26 ES ES384026A patent/ES384026A1/en not_active Expired
- 1970-09-26 ES ES384025A patent/ES384025A1/en not_active Expired
- 1970-12-16 FR FR7045429A patent/FR2081316B1/fr not_active Expired
- 1970-12-26 ES ES70384027A patent/ES384027A1/en not_active Expired
-
1971
- 1971-09-14 SE SE7111629A patent/SE400291B/en unknown
-
1972
- 1972-02-14 CH CH79172A patent/CH538540A/en unknown
-
1973
- 1973-01-29 NO NO73361A patent/NO131386C/no unknown
-
1979
- 1979-03-13 JP JP2909779A patent/JPS5622278B1/ja active Pending
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