IE44476L - Measuring ethanol in a body fluid - Google Patents
Measuring ethanol in a body fluidInfo
- Publication number
- IE44476L IE44476L IE770048A IE4877A IE44476L IE 44476 L IE44476 L IE 44476L IE 770048 A IE770048 A IE 770048A IE 4877 A IE4877 A IE 4877A IE 44476 L IE44476 L IE 44476L
- Authority
- IE
- Ireland
- Prior art keywords
- body fluid
- ethanol
- oxygen
- jan
- buffer
- Prior art date
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title abstract 8
- 210000001124 body fluid Anatomy 0.000 title abstract 5
- 239000010839 body fluid Substances 0.000 title abstract 5
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 abstract 2
- 108010025188 Alcohol oxidase Proteins 0.000 abstract 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 229910052760 oxygen Inorganic materials 0.000 abstract 2
- 239000001301 oxygen Substances 0.000 abstract 2
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 abstract 1
- NUIURNJTPRWVAP-UHFFFAOYSA-N 3,3'-Dimethylbenzidine Chemical compound C1=C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 NUIURNJTPRWVAP-UHFFFAOYSA-N 0.000 abstract 1
- 241000221198 Basidiomycota Species 0.000 abstract 1
- 229940123748 Catalase inhibitor Drugs 0.000 abstract 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 abstract 1
- 241001149671 Hanseniaspora uvarum Species 0.000 abstract 1
- 102000003992 Peroxidases Human genes 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000000872 buffer Substances 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 230000000536 complexating effect Effects 0.000 abstract 1
- 229910001882 dioxygen Inorganic materials 0.000 abstract 1
- 229910021645 metal ion Inorganic materials 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 230000001590 oxidative effect Effects 0.000 abstract 1
- 230000036284 oxygen consumption Effects 0.000 abstract 1
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 1
- 150000002978 peroxides Chemical class 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
1507810 Determination of ethanol in body fluid CHEMBRO HOLDINGS (PTY) Ltd 12 Jan 1977 [15 Jan 1976] 01070/77 Heading G1B Ethanol in a body fluid is determined by a method comprising providing a predetermined volume of body fluid, oxidizing ethanol in the body fluid using an alcohol oxidase in buffer solution in the presence of excess molecular oxygen and measuring the rate of oxygen consumption using for example an oxygen electrode, the oxidation taking place in the presence of an agent operative to suppress the formation of free oxygen by decomosition of peroxide. The agent may be a peroxidase together with o-tolidine, o-dianisidine, aminoantipyrine, or alternatively a catalase inhibitor such as sodium azide. The alcohol oxidase may be obtained from Kloeckera yeast or from a Basidiomycete. The buffer may be a 0À01 to 2 m potassum phosphate buffer pH 7 to 9, and the reaction mixture may contain 10 microns to 10 mm of a metal ion complexing substance such as EDTA-Na.
[GB1507810A]
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ZA760233A ZA76233B (en) | 1976-01-15 | 1976-01-15 | Measurement of alcohol levels in body fluids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE44476L true IE44476L (en) | 1977-07-15 |
| IE44476B1 IE44476B1 (en) | 1981-12-16 |
Family
ID=25569865
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE48/77A IE44476B1 (en) | 1976-01-15 | 1977-01-11 | Measurement of alcohol levels in body fluids |
Country Status (11)
| Country | Link |
|---|---|
| JP (1) | JPS5288394A (en) |
| AU (1) | AU505647B2 (en) |
| BE (1) | BE850383A (en) |
| CA (1) | CA1085279A (en) |
| DE (1) | DE2701168A1 (en) |
| FR (1) | FR2338495A1 (en) |
| GB (1) | GB1507810A (en) |
| IE (1) | IE44476B1 (en) |
| NL (1) | NL7700340A (en) |
| SE (1) | SE7700261L (en) |
| ZA (1) | ZA76233B (en) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1157399A (en) * | 1979-06-05 | 1983-11-22 | Thomas R. Hopkins | Alcohol oxidase from pichia-type yeasts |
| US4540668A (en) * | 1979-06-05 | 1985-09-10 | Phillips Petroleum Company | Alcohol oxidase from Pichia-type yeasts |
| US4401122A (en) * | 1979-08-02 | 1983-08-30 | Children's Hospital Medical Center | Cutaneous methods of measuring body substances |
| DE3477812D1 (en) * | 1983-01-12 | 1989-05-24 | Alcoholism & Drug Addiction | Rapid analysis of ethanol in body fluids |
| JPS59232097A (en) * | 1983-05-16 | 1984-12-26 | Shokuhin Sangyo Center | Method and apparatus for determination of freshness |
| US4734360A (en) * | 1983-07-12 | 1988-03-29 | Lifescan, Inc. | Colorimetric ethanol analysis method and test device |
| US4556635A (en) * | 1983-08-29 | 1985-12-03 | Phillips Petroleum Company | Determination of alcohol content in water imiscible organic systems |
| EP0214336A1 (en) * | 1985-09-13 | 1987-03-18 | Phillips Petroleum Company | Determination of alcohol content in water immiscible organic systems |
| AT390803B (en) * | 1988-08-24 | 1990-07-10 | Avl Verbrennungskraft Messtech | METHOD FOR DETERMINING THE CONCENTRATION OF AN ENZYME SUBSTRATE AND SENSOR FOR IMPLEMENTING THE METHOD |
| US5264106A (en) * | 1988-10-07 | 1993-11-23 | Medisense, Inc. | Enhanced amperometric sensor |
| ATE131528T1 (en) * | 1988-10-07 | 1995-12-15 | Medisense Inc | AMPEROMETRIC SENSOR |
| DE3934299C1 (en) * | 1989-10-13 | 1990-10-25 | Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig, De |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1351547A (en) * | 1971-03-22 | 1974-05-01 | Wothington Biochemical Corp | Determination of the ethanol content of a material |
-
1976
- 1976-01-15 ZA ZA760233A patent/ZA76233B/en unknown
-
1977
- 1977-01-07 CA CA269,354A patent/CA1085279A/en not_active Expired
- 1977-01-11 IE IE48/77A patent/IE44476B1/en unknown
- 1977-01-12 GB GB1070/77A patent/GB1507810A/en not_active Expired
- 1977-01-12 AU AU21261/77A patent/AU505647B2/en not_active Expired
- 1977-01-12 SE SE7700261A patent/SE7700261L/en unknown
- 1977-01-13 DE DE19772701168 patent/DE2701168A1/en not_active Withdrawn
- 1977-01-13 NL NL7700340A patent/NL7700340A/en not_active Application Discontinuation
- 1977-01-14 JP JP349177A patent/JPS5288394A/en active Pending
- 1977-01-14 BE BE174080A patent/BE850383A/en unknown
- 1977-01-14 FR FR7701081A patent/FR2338495A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| BE850383A (en) | 1977-07-14 |
| AU2126177A (en) | 1978-07-20 |
| DE2701168A1 (en) | 1977-07-21 |
| IE44476B1 (en) | 1981-12-16 |
| FR2338495A1 (en) | 1977-08-12 |
| CA1085279A (en) | 1980-09-09 |
| NL7700340A (en) | 1977-07-19 |
| SE7700261L (en) | 1977-07-16 |
| GB1507810A (en) | 1978-04-19 |
| JPS5288394A (en) | 1977-07-23 |
| AU505647B2 (en) | 1979-11-29 |
| ZA76233B (en) | 1977-08-31 |
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