IE48406B1 - Pharmaceutical and dietary composition comprising ypsilon-linolenic acids - Google Patents

Description

This invention relates to compositions for use in the treatment of certain diseases and disorders primarily, but not exclusively, in the field of human medicine.

Considerable interest has been shovzn in recent years in the 5 use of prostaglandin (PG) precursors in medicine.

For various reasons it is not practical to administer naturally-occurring prostaglandins suoh as PGE 1 and PGE 2 to patients. Consequently, considerable attention has focussed on the use of prostaglandin precursors including linoleic acid, γ-linolenic acid (GIA) and dihomo-y-linolenic acid (DGIA).

Conversion of these materials in the body is believed to be as shown In the following diagram: Linoleic Acid (9,12-octadecadienoic acid) GLA (6.9,12-octadecatrienoic acid) Penicillin D5LA e^c' DGLA —»~1 series ester (5.8.11-eicosatrienoic acid) pg’s reserves « / (small) 1 / ©Feedback control Possible© ,, , I feedback l-ar9e I ΔΔ ι control (Arachidonic acid.ie. \ reserves 5,8.11,1 t-eicosatetraenoicacid) Feedbackx n ... con trot Possible ©feedback control TXA2 [Thromboxane Δ2) I \ PGI2 PGE2etc. sprips ndoperoxides Series PG s The broad outline of this pathway is well known, but the details of control,inhibition and enhancement are shown as the present inventor believes them to operate. The pathway is now discussed with particular reference to treatment of cancers and inflammatory disorders. This discussion is given in the belief that it elucidates the invention, but it is not intended that the invention should be limited by what is believed to be the reason for its effectiveness.

A major function of essential fatty acids (EFAs) is to act as precursors for prostaglandins (PGs), 1 series PGs being formed from dihomo-y-linolenic acid (DGLA) and 2 series PGs from arachidonic acid (AA). DGLA and AA are present in food in only small quantities, and the major EFA in food is linoleic acid which is first converted to γ-linolenic acid (GLA) and then to DGLA and AA. The conversion of linoleic acid to GLA is blocked by a high fat and high carbohydrate diet, by ageing and by diabetes. Stores of AA in the body in the form of lipid esters are very large indeed. In contrast only small amounts of DGLA ester are present.

There is evidence to show that in cancers, there is an over20 production of prostaglandins, abnormal calcium levels and a switch from an oxidative mode of metabolism to a glycolytic mode, which the present inventor believes may be due in part to a defect in the synthesis of TXA2. Such a defect may be caused for example by radiation. Moreover it is also believed that in an organism TXA2 binds to chromosomes and thereby increases their resistance to mutagens.

Thus, it is suggested, cancer involves two mechanisms, firstly a reduction ln TXA2 levels and secondly an exposure to a mutagenic agent which leads to uncontrolled growth.

Therefore, many of the problems of cancer may be caused by the primary failure of TXA 2 synthesis and the secondary excess of prostaglandins of the 2 series and depletion of prostaglandins of the 1 series and essential fatty acids.

It has further recently been found that a critical factor in sone inflammatory disorders, e.g. in the damage of myelin Which occurs in multiple sclerosis, may be the entry of calcium into cells. This may damage mitochondria and activate destructive lysosomal enzymes. Thus, there is now evidence which indicates that the regulation of the immune response and also the control of intracellular calcium may be significant factors in the treatment of various inflammatory disorders, e.g. multiple sclerosis, Crohn's disease and other disorders listed below.

The present inventor has now found that colchicine is a substance which appears to be able to potentiate the removal of calcium by cells and thus may be able to control intracellular calcium. Colchicine may also inhibit formation of 2 series PG's and enhance formation of 1 series PG's. In a further aspect of the invention, therefore, in conjunction with correction in EPA balance, colchicine is administered to effect such control. The relationship of this to EPA metabolism is discussed later.

In cancers as discussed above, and in inflammatory disorders, production of 2 series PGs from arachidonic acid is greatly exaggerated. In inflammatory disorders these PGS are thought to contribute to the causation of the disease because steroids and aspirin-like drugs are both partially effective therapies, steroids blocking the conversion of AA esters to free AA and aspirin-like drugs blocking the conversion of free AA to endoperoxides which are intermediates in PG synthesis. As yet there is less evidence that the increased formation of 2 series PGs plays an important part in cancer but some human tumours are known to respond to steroids and growth of some animal tumours is inhibited by aspirin-like drugs.

The overproduction of 2 series PGs implies that normal control of the PG synthetic pathway has been lost. Although control of this pathway is imperfectly understood two factors have been identified. 1. PGE1 is able to inhibit the formation of free AA from AA esters.

This leads to the paradoxical fact that a partial EFA deficiency actually leads to increased formation of 2 series PGs, because DGLA stores are so much smaller than those of AA and a partial deficiency of EPAs will therefore lead to DGLA depletion first. This depletion will reduce formation of PGE1, remove the PGE1 control of AA and allow overproduction of 2 series PGs from the large AA stores. 2. An unstable product of AA metabolism, thromboxane A2 (TXA2), also feeds back to inhibit conversion of AA ester to free AA and possibly also of free AA to PG2 endoperoxides. Thus loss of TXA2 will also lead to overproduction of 2 series PGs.

TXA2 and PGE1 thus cooperate in the regulation of formation of 2 series PGs and a fault in the formation of either will lead to abnormalities.

Thus for example the disorders of PG synthesis in inflammatory disorders and cancer can be accounted for by inadequate formation of PGE1 and/or TXA2.

The evidence for direct involvement of PGs in inflammatory disorders and cancer has been briefly mentioned. There is also indirect evidence that PGs may act by regulating - or failing to regulate - the calcium movements into and out of cells already mentioned above. The calcium concentration in cytoplasm is normally very low and there is now excellent evidence from many sources that a brief rise in cytoplasmic calcium concentration triggers a variety of cell events, including cell division and activation of lysosomes which contain destructive enzymes. Normally this calcium is very rapidly removed after this brief activation so terminating the event. PGs and related substances have specific actions on calcium and the present inventor has obtained evidence to suggest that TXA2 and PGF2a may be of critical importance. In particular, specific inhibition of TXA2 synthesis greatly prolongs the time taken for calcium to be removed from the cytoplasm after activation.

Furthermore inhibition of TXA2 synthesis leads to increased formation of PGF2a · and PGE2 which can promote calcium entry into cells.

There is thus good evidence that in this respect also PGE1 and TXA2 enhance one another's effects. In particular, in muscle the degree of contraction is related to the calcium concentration in the cytoplasm and muscle contraction is a measure of this calcium concentration. After inhibition of TXA2 synthesis the recovery from a contraction is greatly prolonged indicating slow removal 8 4 0 8 of calcium. Further, inhibition of TXA2 synthesis can lead to a chronic state of partial contraction indicating the entry of calcium into the cytoplasm. PGF2a and PGE2 whose output is increased by inhibition of TXA2 synthesis also cause contraction indicating calcium entry into the cytoplasm.

Thus loss of TXA2 and PGE1 synthesis will lead to increased formation of 2 series PGs and entry of calcium into the cytoplasm. This calcium may activate cell division and also activate lysosomes whose destructive enzymes may play a large part in inflammation.

There is a good deal of evidence that cancers do indeed not produce TXA2 normally. The most striking is as follows: (a) Specific inhibitors of TXA2 synthesis, such as imidazole, can produce in normal cells biochemical abnormalities similar to those in naturally occurring cancers. (b) Radiation and phorbol esters, which powerfully promote the development of cancers, are both able to inhibit the enzyme which forms TXA2.

The evidence of defective 1 series PG synthesis in czuicer is less substantial at present. However, rapidly growing cancers frequently produce skin lesions in their hosts which are identical to those caused by 1 series PG deficiency. Further, in rat breast cancer there is evidence that synthesis of α-lactalbumin is regulated by PGE1, and o-lactalbumin synthesis fails as the breast tissue is transformed from the normal to the cancerous state.

There is suggestive evidence that TXA2 may be able to protect DNA from mutations. For example the phorbol esters do not cause mutations themselves but they do make cells much more susceptible to other mutagenic agents, or more particularly, the expression of their effect. It is possible that even when a mutation has taken place, it may not be expressed if adequate amounts of TXA2 are present. For example rats can be exposed to mutagenic radiation at birth but develop cancers only on administration of phorbol esters up to a year later.

On general grounds there are therefore reasons to suppose that suppression of excess production of 2 series PGs will have desirable effects in both inflammatory disorders and cancer. 8 4 0 6 Currently available conventional methods of suppression axe administration of steroids and aspirin-like drugs. However, while these may suppress overproduction of 2 series PGs they will exaggerate further any deficiencies in PGs of the 1 series and in TXA2, which may explain why they control symptoms but do not usually alter the long term course of the disease.

The present invention proposes a radically new approach which will control excess PG2 series production by restoring towards normal, or enhancing, the formation of either or both of 1 series PGs and TXA2.

The methods proposed for doing this are as follows: 1-Series PGs To increase the available supply of precursors of 1-series PGs by providing adequate amounts of GIA or DGIA which will by-pass any metabolic block between IA and GIA. The GIA or DGIA may be either synthetic or found in natural products. The formation of 1 series PGs may be enhanced further by the administration of pharmacological agents with the GIA or DGIA. Agents which have this effect are listed later in the specification. They include penicillamine and levamisole which have both been used as antiinflammatory agents in rheumatoid arthritis with a completely unknown mechanism of action.

TXA2 To enhance the formation of TXA2 by means of agents which specifically activate the enzyme which forms TXA2 from PG2 series endoperoxides. These agents also are listed later in the specification, and include colchicine and related compounds such as the Vinca alkaloids.

These latter should be used in much lower doses than those at present used in cancer therapy, since high doses may have the reverse effect of inhibiting TXA2 formation. One has the apparently paradoxical situation that colchicine and the Vinca alkaloids may attack cancer in one of two ways, tow doses, according to the invention, activate TXA2 synthesis, inhibit formation of other 2 series PGs and restore calcium regulation.

They will therefore tend to normalise cancer cells. High doses on the other hand, aa given in known treatments, seem to be toxic to the enzyme. They therefore eliminate any remaining TXA2 synthesis, further enhance formation of PGF2a and other 2 series PGs and kill the cells by increasing calcium entry to the toxic level the effect nevertheless being sufficiently selective for cancer cells, to be of value.

It may be remarked that radiation also has apparently paradoxical effects which are explained on the concept on which the invention is based. Sub-lethal irradiation of normal cells inactivates TXA2 synthesis, opening the way to the abnormalities seen in cancer. Irradition of cells in which TXA2 synthesis is already defective kills the cells by overloading them with calcium, the effect therefore being selective to cancer cells.

Direct evidence of effectiveness in cancer and inflammatory disorders treatments is given at the end of the specification.

There now follows some references to prior proposals for the use of γ-linolenic acid and like materials in medicine, and then a detailed statement of the present invention.

Prior art within this general area includes the following patents and papers. (i) U.S. Patents Nos. 3 993 775 (issued November 23rd, 1976) and 4 058 594 (issued November 15th, 1977) of John Williams, which describe a method of providing an imrauno-suppressive effect in a patient undergoing organ or tissue transplant or suffering from multiple sclerosis comprising administration of a daily dosage of from 5 mg to 3 g of γ-linolenic acid or dihomo-y-linolenic acid or a functional derivative thereof. (ii) British patent Specification No, 1 082 624 published September 6th, 1967 (Calmic Limited), which discloses effectiveness of γ-linolenic acid in the treatment of vascular diseases. (iii) McCormack, Neil and Sim (The Lancet, page 308, September 3rd, 1977) who describe preliminary work on the use of an oil containing a mixture of linoleic acid and γ-linolenic acid (as triglycerides) in the treatment of rheumatoid arthritis. (iv) Sim and McCraw (Thrombosis Research Volume 10, pages 385-397, 8 4 0 8 1977), who describe activity of the methyl esters of γ-linolenic acid and dihamo-y-linolenic acid in vitno and in vivo on blood platelet function in ncn-human primates and in man. (v) Zurier and Quagliata (Nature 234: 304, 1971), who describe the inhibitory effect of PGE1 an adjuvant arthritis in rats. (vi) Zurier, Sayadoff, Torrey and Rothfield (Arthritis Rheum 20: 723, 1977) who describe the inhibitory effect of PGE1 on a naturally occurring inflammatory disease in mice which resembles human systemic lupus erythematosus. (vii) Tolnai (Can. J. Physiol. Pharmacol. 44 No. 2 339—343, 1966) who tested more than 100 fatty acids and derivatives including linoleic acid and an unspecified isoner of linolenic acid as potential antitumour agents.

In the book The Pharmaceutical Basis of Therapeutics, Ed. Goodman and Gilman (Macmillan, 3rd Ed 1965) pages 1374—1376, the uses and effects of the Vinca alkaloids are discussed.

In the light of the general discission above) and the present inventor's earlier Patent Specification No?47777 , the present invention in its various aspects, using γ-linolenic acid and/or dihcmo-y-linolenic acid, optionally in association with linoleic acid and if desired other fat acids, said adds being used, if desired, as physiologically functional derivatives thereof, may be stated as follcws: A. A pharmaceutical composition comprising γ-linolenic acid or other material as above in conjunction with (a) a material enhancing the synthesis or action of TXA2, or (b) both such a material and a material affecting the l-series/2-series PG balance in the body in favour of 1-series PGs, in an acceptable pharmaceutical vehicle.

Compositions of, for example, γ-linolenic add in con30 junction with zinc and/or β-lactam antibiotics, which are believed to give such 1-series PG enhancement, are the subject, inter alia, of the above earlier patent application, B. A pharmaceutical composition comprising γ-linolenic add or other material as above, in conjunction with (a) colchicine, griseofulvin, vinblastine, vincristine and other Vinca alkaloids, amantadine, melatonin (pineal hormone) or interferon, and optionally (b) zinc, a β-lactam antibiotic, penidllamtine, phenforman or levamisole in an acceptable pharmaceutical vehicle. 40 6 The γ-linolenic acid or dLhciro-Y-linolenic acid may of course be present in the form of physiological functional derivatives thereof, that is to say physiologically acceptable salt, ester or other derivative convertible in the body to the acids and having the same effect hy entering the biochemical pathway set out earlier.

In particular a ccopositian for treating cancer may use (a) γ-linolenic acid and/or dUxano-Y-linolenic acid, if desired in association with linoleic acid, said acids being used, if desired, as physiologically functional derivatives thereof; (b) an effective amount of a substance selected from zinc, penicillin and penicillamine; and (c) an effective amount of a substance selected from colchicine, vinblastine, vincristine, griseofulvin, interferen and amantadine. Penicillin is a representative of β-lactam antibiotics, and other effective materials are phenformin and levamisole.

INEEAM^TDRY DISORDERS TREATED lhe disorders that can be treated include multiple sclerosis; systemic lrpus erythematosus; Crohn’s disease; ulcerative colitis; inflanmatory diseases of the kidney, for exanple, glcmerulo-nephritis and nephrotic syndrome; inflammatory and degenerative diseases of the nervous and muscular systems, for exanple, muscular dystcphies, Friedreich's ataxia and related conditions of peripheral nerve degeneration; disorders of an auto-immune nature; and other collagen related diseases; rheumatoid arthritis and other inflanmatory joint disorders; inflanmatory skin disorders; disorders characterised by recurrent inflammation such as Familial Mediterranean Fever or Behcet's Syndrome.

PACKS If it is not desired to have compositions comprising active materials listed above, packs may be prepared comprising the materials presented for separate or pmart joint and pmart separate administration in the appropriate relative amounts, and such packs are within the purview of the invention.

DIETARI OOMPOSITICMS The invention is chiefly described in terms of pharmaceutical compositions, but it will be understood that the γ-linolenic and other acids being in the nature of dietary sipplerments, could be incorporated in a dietary margarine or other foodstuffs; such foodstuffs, possibly containing other active materials and generally referred to in this description as dietary or pharmaceutical 8 4 0 6 compositions, are within the purview of the invention and thus of the term pharmaceutical compositions ear packs used iii the claims.

VETERINARY APPLICATIONS It will be understood that where a disorder of a kind calling for treatment in animals arises, the invention while described primarily in terms of human medicine and treatment is equally applicable in the veterinary field.

AMOUNTS OP ACTIVE MATERIALS Amounts of zinc and β-lactam antibiotics are given later in general discussion of those materials.

Amounts of the alternative materials penicillamine, phenformin and levamisole are Penicillamine 50 mg to 10„g/day Phenformin 10 mg to 5 g/day Levamisole 10 mg to 2 g/day For colchicine, based on present evidence, a suitable dose regimen for inflammatory disorders involves the administration of from 0.3 to 15, for example 0.6 to 2.4 mg, of colchicine per day. Other materials as listed with colchicine below, if used for inflammatory disorders, may be in the amounts there given.

For cancer the administration of colchicine, vinblastine, vincristine, griseofulvin, interferon or amantadine may conveniently be in the following amounts. colchicine amantadine griseofulvin vinblastine vincristine interferon (by injection) 0.3 to 15 mg/day 100 to 1000 mg/day 0.5 to 5 g/day 0.5 to 5 mg/kg/week (average weight 70 kg) o.l to 1.0 mg/kg/week (average weight 70kg) c Q x 10 to 1 x 10 units/day melatonin 10 mg to 5 g/day For convenient administration the above amounts in a half a third or a quarter thereof rtey be presented in dosage form, giving for example doses of 0.125 to 15 mg colchicine and similarly for the other materials.

AMOUNTS CF γ-LMXENIC AND OTHER ACIDS SEECTFICSELY A preferred daily dosage for both cancer and inflammatory disorders for an adult (weight ca 75 kg) is from 0.05 or 0.1 up 4-8Λ0 6 to 1, 2, 5 or even 10 g as required of γ-linolenic acid or equivalent weight (calculated as γ-linolenic acid) of physiologically functional derivative thereof. Amounts may in particular be 0.1 to 1.0 g daily. Such doses correspond to about 2 to 20 g daily of the' Oenothera oil discussed below. In place of, or in addition to, γ-linolenic acid, one may use dihomo-y-linolenic acid or a physiologically functional derivative thereof, in amounts equivalent in molar terms to γ-linolenic acid and calculated as such. This dosage can for example be taken as a single dose or divided into 2, 3 or 4 subdivisions thereof as convenient.

Based on present evidence, a particularly suitable daily dosage in cancer treatment for an adult (weight ca 75 kg) would be from 0.15 to 1.5 g of γ-linolenic acid or equivalent weight of functional derivative thereof.

Again based on present evidence, a particularly suitable daily dosage in inflammatory disorders for an adult (weight ca 75 kg) would be from 0.1 to 1.0 g of γ-linolenic acid or equivalent weight of functional derivative thereof.

FORMS AND SOURCE OF -y-I.INOr.ENIC AND OTHER ACIDS Convenient physiologically functional derivatives of γ-linolenic acid and dihoao-y-linolenic acid for use according to the invention for all the purposes described include the C^-C^ alkyl (e.g. methyl and ethyl) esters and the glycerides of the acids.

If desired, pharmaceutical compositions may be produced for use in the invention by associating natural or synthetic γ-linolenic acid (or a physiologically functional derivative thereof) and/or dihomoy-linolenic acid (or a physiologically functional derivative thereof) as such, with an acceptable pharmaceutical vehicle. It is at present convenient to incorporate the γ-linolenic acid into compositions in the form of an available oil having a high γ-linolenic acid content.

At the present time known natural sources of oils having a high γ-linolenic acid content are few (there are no known natural sources of significant amounts of dihomo-y-linolenic acid).

One source of oils currently available is the seed of Evening Primrose species such as Oenothera biennis D. and Oenothera lamarcklana, the oil extract therefrom containing γ-linolenic acid (about 8%j and linoleic acid (about 72%) in the form of their glycerides together 8 4 0 6 with other glycerides (percentages based on total fatty acids). Another source of γ-linolenic acid is the seed of Borage species such as Borago officinalis which, though its current yield per acre is low, provides a richer source of γ-linolenic acid than Oenothera oil. Recent studies on fungi which can be cultivated by fermentation promise a fungal oil source.

The seed oil extracts referred to above can be used as such or can for example if desired be fractionated to yield an oily composition containing the triglycerides of γ-linolenic acid and linoleic acid as the main fatty acid components, the γ-linolenic acid content being if desired a major proportion. Seed oil extracts appear to have a stabilising effect upon any dihomo-y-linolenic acid or physiologically functional derivative thereof incorporated therein. USE OF ZINC Without restriction to the theory, it is believed that zinc tends to stimulate the biosynthesis of 1 series PG's and specifically that it potentiates mobilisation of esterified reserves of dihonio-γlinolenic acid. This enables one to use zinc conjointly with γ-linolenic acid and/or dihomo-Y-linolenic acid. The presence of arachidonic acid or any other material tending to oppose the PGI enhancing effect is, naturally, to he avoided.

Based on present evidence, a suitable daily dosage for an adult (weight ca 75 kg) is 2.5-800 mg preferably 10-200 mg and advantageously 10-80 mg zinc daily, with γ-linolenic acid or other acid or equivalent in the amounts previously discussed. The 10-80 mg zinc is approximately 0.125-1.0 mg/kg adult body weight. In view of the conjoint effect of the zinc preferred amounts of γ-linolenic or other acid or equivalent are less than when zinc is not present, advantageously 0.1 to 1.0 g daily. As before the dosage can be taken as a single dose or divided into 2, 3 or 4 subdivisions thereof.

Conveniently the zinc and γ-linolenic or other acid or derivatives are given together in a single preparation but they can of course be taken separately.

The zinc should be administered in a form in which it is readily taken up in vivo. Ordinarily this will indicate the use of a zinc salt of a mineral or organic acid, said salt being physiologically acceptable at the given dosage. Some zinc salts which would be contraindicated at higher dosages may be satisfactory for present purposes at the dosages indicated above. Useful salts include zinc sulphate and zinc gluconate and in particular zinc oleate, γ-linolenate and dihomo-Y-linolenate, and zinc oxide may also be employed. It is also possible to administer the zinc in chelated form. In any event, the preferred amounts of zinc are as stated above (the quantities given being calculated as zinc metal). Zinc oleate may be made by the method disclosed in Monatschrift 42 287 (1921, and similar methods may be applied to make for example zinc γ-linolenate if desired.

EXPERIMENTAL WORK ON USE OF ZINC In one group of experiments the test preparation was the isolated superior mesenteric vascular bed, taken from male rats as for example described in the Canadian J. Physiol Pharmacol 54: 357, 1976. The perfusion flow rate was at a constant value between 3 to 4 ml/min., pressure 25 to 30 mm Hg, using Krebs bicarbonate buffer containing in nM 150 Na, 4.3 K, 1.0 Mg, 2.5 Ca, 1.7 phosphate, 25 bicarbonate and 11.1 glucose.

Prior to testing the basic vasoconstrictive effect of norepinephrine as the bitartrate, in successive 10 ng amounts was established, as the amplitude of a transient rise of about 1 min in the perfusion pressure.

Zinc, as the chloride, was then added to the perfusion buffer at successive concentrations and the norepinephrine response measured after 15 minutes at each.

The following results were obtained: Zinc concentration (ug/ml) Response as 4 of basic level 0.1 112 0.2 118 0.4 130 0.8 138 In the presence of 50 pg/ml of indomethacin, a known blocking agent for PG synthesis, used with 10 ng/ml PGE2 to give apparently normal vascular reactivity, the zinc had no effect on the norepinephrine response. 8 4 0 6 Similar tests with dihomo-y-linolenic acid and PGE1 gave respective rises up to a maximum of 130% of the basic response at 50 ng/ml of the acid and a maximum of 150% of the basic response at 2.8 x 10 M PG.

The results show that zinc gives responses like those of dihomo-y-linolenic acid and of PGE1, responses moreover which are not given when PG synthesis is blocked and PGE2 supplied, and thus the conditions treated with γ-linolenic acid (and thus effectively with dihomo-y-linolenic acid) may be enhanced in the direction of 1 series PG synthesis by the addition of zinc.

Analogous experiments with the same preparation show that phenformin, levamisole, penicillin and penicillamine have actions consistent with stimulation of PGE1 synthesis.

USE OF ZINC WITH OTHER MATERIALS As shown above, in the perfused mesenteric vascular bed of the rat, zinc appears to increase the formation of PGE1 from DGLA.

The presence of either colchicine (100 ng/ml) or melatonin (10 ng/ml) in the perfusion fluid increases the effect of zinc on PGE 1 by 10 to 100 times, the size of the effect depending on the time of the year and being greater in the summer months than in the winter. This is probably because the production of melatonin from the pineal gland is lower in the summer than in the winter and the effect of extra melatonin can therefore be more easily seen in the summer.

Colchicine and melatonin appear to act at the same sites in cells, and their overall effect therefore is to increase the formation of PGE1. The effect is believed to be mediated at least in part by the effect of colchicine and melatonin on thromboxane A2.

The alternative materials to colchicine previously mentioned may be expected to have similar effects.

USE OF β-LACTAM ANTIBIOTICS β-lactam antibiotics which may be used according to the present invention, are conveniently any of known penicillin and cephalosporin antibiotics (including semi-synthetic antibiotics) such as, for example, penicillin G, penicillin N, penicillin V, cephalexin, cephalothin, ampicillin, amoxycillin, cloxacillin and cephaloglycin. Any of these may be used in the form of their physiologically functional non-toxic derivatives, for example alkali metal salts e.g. sodium and potassium salts, and salts with organic bases, and reference to an antibiotic herein (including the claims) includes reference to such derivatives.

Suitable daily dosages may for example be in the range 0.5 to 10.0 g per day in patients of average weight. Such daily dosages may conveniently'be divided as for zinc.

The use of penicillins in long term treatments is especially desirable in view of the known relative absence of side effects of these drugs. Thus, penicillin has been administered for many years to patients having rheumatic heart disease in order to prevent streptococcal Infections, and there is virtually no evidence of long term toxicity.

Care should of course be taken to ensure that the patient is not allergic to the drug of choice.

It is believed that the reason for the effectiveness of the. antibiotics In certain disorders is that they enhance utilisation of ester reserves of dihomo-Y-linolenic acid. Whether or not this is so, and no restriction to the theory is intended, zinc and antibiotics do appear to have parallel effects in treating all the conditions discussed herein when used with the γ-linolenic or other acids and derivatives.

In particular in tests carried out on the rat mesenteric bed system as above, both penicillin V and penicillin G have given responses similar in kind and degree to those given for zinc, supporting further inventor's belief that β-lactam antibiotics are of value in all other conditions treated according to the invention in similar way to the action of zinc. It may be expected that colchicine will enhance the effect of antibiotics just as It enhances the zinc effect.

PHARMACEUTICAL PRESENTATION The compositions according to the invention are conveniently in a form suitable for oral, rectal, parenteral or topical administration in a suitable pharmaceutical vehicle, as discussed in detail for exanple in Patent No. . 29488 and in any case 484G6 very well known generally for any particular kind of preparation. Thus for example tablets, capsules, ingestible liquid or powder preparations, creams and lotions for topical application, or suppositories, can be prepared as required. Injectable solutions of hydrolysed Oenothera oil may be prepared using albumin to solubilise the free acid.

Advantageously a preservative is incorporated into the preparations. α-Tocopherol in a concentration of about 0.1% by weight has been found suitable for the purpose.

It will be understood that the absolute quantity of active ingredients present in any dosage unit should not exceed that appropriate to the rate and manner of administration to be employed but on the other hand should also desirably be adequate to allow the desired rate of administration to be achieved by a small number of doses. The rate of administration will moreover depend on the precise pharmacological action desired.

The following Examples serve to illustrate pharmaceutical compositions useful in treatment according to the invention:EXAMPLES Pharmaceutical compositions containing a unit dose of an oil extract from the seeds of Oenothera biennis I·, optionally with methyl dihomo-y-linolenate and/or zinc sulphate and/or penicillin V and/or any of the other active materials referred to herein are prepared by encapsulation of the natural oil in soft gelatin capsules manufactured by known methods.

The oil is extracted from the seeds by one of the conventional methods of extraction such as cold pressure, screw pressure after partially cooking the seed, or solvent extraction.

Fractionation of a typical sample of this oil shows a yield of 97.0% oil in the form of methyl esters, with the relative proportions: Palmitate 6.15 Stearate 1.6 Oleate 10.15 Linoleate 72.6 γ-Linolenate 8.9 As preservative, α-toccpherol is added to the oil in a ocncentratiox of 0.1%.

Gelatin capsules containing oil extracts prepared as described above, each having the following contents of active ingredients (0.5 g oil extract - ca 0.045 g γ-linolenic add), are prepared in conventional fashion.

EXAMPLE 1 Oil extract 0.5 g Colchicine 0.15 mg One capsule may be administered four times daily in the treatment of cancer or multiple sclerosis and other inflammatory disorders as described above.

Oil extract EXAMPLE 2 0.5 g Methyl dihomo-y-linolenate 10 mg Colchicine 0.3 mg One capsule may be administered four times daily in the treatment of cancer or multiple sclerosis and other inflammatory disorders as described above. EXAMPLE 3 Oil extract 0.5 g Colchicine 0.25 mg Penicillin V 0.25 g One or two capsules may be administered four times daily in the treatment of cancer or multiple sclerosis and other inflammatory disorders as described above.

EXAMPLE 4 Oil extract 0.5 g Colchicine 0.25 mg Zinc oleate 20 mg One or two capsules may be administered four times daily in the treatment of cancer or multiple sclerosis and other inflammatory disorders as described above.

EXAMPLE 5 Oil extract Phenformin Amantadine 0.5 g 25 mg 100 mg One or two capsules may be administered four times daily in the treatment of cancer or multiple sclerosis and other inflammatory disorders as described above.

EXAMPLE 6 Oil extract 0.5 g Colchicine 0.25 mg Levamisole 25 mg One or two capsules may be administered four times daily in the treatment of cancer or multiple sclerosis and other inflammatory disorders as described above.

EXAMPLE 7 Oil extract 0.5 g Colchicine O.25 mg Penicillamine 100 mg One or two capsules may be administered four times daily in the treatment of cancer or multiple sclerosis and other inflammatory disorders as described above.

EXAMPLE 8 Oil extract 0.5 g Griseofulvin 0.5 mg One capsule may be administered four times daily in the treatment of cancer or multiple sclerosis and other inflammatory disorders as described above.

EXAMPLE 9 Oil extract (0.5 g) capsules or oil extract (0.5 g) plus zinc 3o sulphate (10 rag) capsules may be administered in doses in two, three tines daily in conjunction with 70 mg/Veek vinblastine.

EXAMPLE 10 Oil extract capsules or oil extract plus zinc capsules may be administered as in Example 9 in conjuction with 70 mg/week vincristine.

EXAMPLE 11 Oil extract capsules or oil extract plus zinc capsules as in Exairple 9 but without vinblastine tcetg be administered in conjunction with 0.5 g/day melatonin.

EXAMPLE 12 Oil extract capsules or oil extract plus zinc capsules as in Example 9 but without vinblastine may be administered in conjunction with 1 χ 10θ units/day interferon.

FURTHER EVIDENCE - CANCER Cancer trials in humans inevitably take several years but the present inventor has shown the potential value of the approach in the R323OAC rat mammary cancer. Administration of Evening Primrose oil can reduce the rate of growth of established cancers to less than half. Colchicine and melatonin have similar effects and the combination of oil and colchicine, melatonin or other materials listed with them in the specification will be even more desirable. These cancers are started by transplantation of minute pieces of tumour tissue, and normally over 904 of these tumours take” and grow, but if Evening Primrose oil is administered before.and immediately after transplantation, less than 40% of the transplants develop into full tumours.

Specifically, 1 mm diameter pieces of the transplantable R323OAC breast tumour were transplanted subcutaneously in rats of the Fisher strain (50 animals). After five weeks the animals were killed and the tumours removed. In 10 control animals which received daily saline injections the mean tumour weight was 1.6 gram. In 10 animals which received 25 microlitres of Evening Primrose oil subcutaneously each day the mean tumour weight was 0.64 g. InlO animals which received 100 microlitres of Evening Primrose oil daily mean tumour weight was 0.46 g. In 10 animals which received 25 microlitres oil plus 10 microg colchicine per day mean tumour weight was 0.32 g. In 10 animals which received 25 microlitres oil plus 1 mg penicillin g per day the mean tumour weight was 0.36 g. The treatments thus substantially slowed tumour growth.

Further, in one male human patient with an ultra-violet radiation induced basal cell carcinoma (rodent ulcer) of the face, administration of Evening Primrose oil (3 ml/day) caused complete disappearance of a 5 mm tumour within 6 weeks. The present inventor knows of no instance of spontaneous regression of this type of tumour.

FURTHER EVIDENCE - INFLAMMATORY DISORDERS 5 In inflammatory disorders in animals PGE1 is able to improve the conditions successfully (prior art (v) and (vi)). It may therefore be expected that agents which enhance endogenous formation of PGE1 will have a similar effect. Indeed this may be how penicillamine and levamisole work in rheumatoid arthritis although this has not been suggested other than by the present inventor.

Dosages in the claims hereafter are daily unless otherwise stated.

Claims (22)

1. OAMSs1. A pharmaceutical carpcsiticn ccnprising (a) γ-linolenic acid and/or dihcno-y-linolenic acid and/or a physiologically acceptable salt, ester or other derivative thereof convertible in the bod/ 5 thereto and (b) a material enhancing the synthesis or action of throifcraxane A2, alone or in an acceptable pharmaceutical vehicle.

2. A pharmaceutical ccnpositian as claimed in claim 1 and ccnprising (a) γ-linolenic acid and/or dihcno-y-linolenic acid and/or a physiologically acceptable salt, ester or other derivative thereof 10 convertible in the body thereto and (b) a material selected fran colchicine, griseofulvin, vinblastine, vincristine and other Vinca alkaloids, amantadine, melatonin, and interferon, alcne or in an acceptable pharmaceutical vehicle.

3. The ccnpositian of claim 1 or 2, presented for administration 15 in doses ccnprising 0.0125 to 10 g of (a) calculated as γ-linolenic acid.

4. The oanpositicn of claim 1 or 2, presented for administration in dases ccnprising 0.025 to 1 g of (a) calculated as γ-linolenic acid. 20 5. The caiposition of any preceding claim, presented for daily or •where noted weekly administration in doses ccnprising: 0.075 to 15 mg colchicine or 25 to 1000 mg amantadine or 0.125 to 5 g griseofulvin or 8.75 to 350 mg vinblastine (weekly) or 1.75 to 70 ng vincristine (weekly) or

5. 8 0.25 x 10 to 1 x 10 units interferon or 2.5 ng to 5 g melatcnin.

6. A pharmaceutical pack comprising (a) and (b) as set out in any preceding claim, presented separately but for conjoint administration.

7. The composition of ary one of claims 1 to 5, comprising 5 further (c) a material affecting the l-series/2-series PG balance in the body in favour of 1-series prostaglandins and selected from physiologically assimilable zinc, penicillamine, phenformin, levamisole or a β-lactam antibiotic.

8. The composition of claim 7, wherein the antibiotic is 10 penicillin G, penicillin N, penicillin V, cephalothin, anpicillin, amoxycillin, cloxacillin, cephalexin, cephaloglycin, or other natural or semi-synthetic penicillin or cephalosporin antibiotic.

9. The composition of claim 7 or 8, presented for administration in doses ecnprising 0.125 to

10. G of tha antibiotic. 15 10. The composition of claim 7, presented for administration in doses cortprising: 12.5 mmg to 10 g penicillamnine or 2.5 mg to 5 g phenformin or 2.5 mg to 2 g levamisole . 20

11. The composition of claim 7, presented for administration in doses comprising 0.625 to 800 mg assimilable zinc calculated as the netal.

12. The composition of claim 7, presented for administration in doses comprising 2.5 to 200 mg assimilable zinc calculated 25 as the metal.

13. The composition of claim 7, presented for administration in doses comprising 2.5 to 80 mg assimilable zinc calculated as the metal.

14. A pharmaceutical or dietary pack canprising (a), (b) and (c), as referred to in any one of claims 7 to 13, presented separately or two together and cne separately, but for conjoint administration.

15. A conposition according to ary preceding claim, wherein the derivative of γ-linolenic acid or dihamo-^-linolenic acid is a methyl or ethyl ester or glyceride thereof.

16. A ccnpositicn according to ary preceding claim, wherein the γ-linolenic acid is present in the form of the oil of the seed of Oenothera biennis L., Oenothera lamarckiana, car other Evening Primrose species, or a fraction thereof.

17. A ccnpositicn according to any preceding claim, wherein the γ-linolenic acid is present in the form of the oil of the seed of Borago officinalis or other Borage species, or a fraction thereof.

18. A ccnpositicn according to any preceding claim specifying the presence of zinc, wherein the zinc is present as a salt of an inorganic acid or of oleic, γ-linolenic, dihomo-y-linolenic or other organic acid, or zinc oxide, or chelated zinc.

19. A conposition according to any preceding claim, comprising further an effective and pharmaceutically acceptable amount of α-toccpherol or other antioxidant.

20. A pharmaceutical ccnpositicn according to claim 1, substantially as hereinbefore described with particular reference to the accompanying Exanples.

21. A pharmaceutical pack as claimed in claim 6, substantially as hereinbefore described.

22. A pharmaceutical or dietary pack as claimed in claim 14, sihstantially as hereinbefore described.