IE810071L - Tandem synthetic genes - Google Patents

Tandem synthetic genes

Info

Publication number
IE810071L
IE810071L IE810071A IE7181A IE810071L IE 810071 L IE810071 L IE 810071L IE 810071 A IE810071 A IE 810071A IE 7181 A IE7181 A IE 7181A IE 810071 L IE810071 L IE 810071L
Authority
IE
Ireland
Prior art keywords
attachment
synthetic gene
asparagyl
phenylalanine
self
Prior art date
Application number
IE810071A
Other versions
IE50833B1 (en
Original Assignee
Searle & Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Searle & Co filed Critical Searle & Co
Publication of IE810071L publication Critical patent/IE810071L/en
Publication of IE50833B1 publication Critical patent/IE50833B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06104—Dipeptides with the first amino acid being acidic
    • C07K5/06113—Asp- or Asn-amino acid
    • C07K5/06121—Asp- or Asn-amino acid the second amino acid being aromatic or cycloaliphatic
    • C07K5/0613—Aspartame
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/222—Phenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)

Abstract

The invention relates to a synthetic gene which contains in the coding strand codons for the amino acids of a required peptide in tandem repeats. The invention further comprises a process of a synthetic gene of this type, which is characterised by self-attachment and ligation and, where appropriate, repair of a plurality of suitable oligonucleotides, where the attachment is achieved with the formation of the repetitive character by self-complementary, overlapping ends at the termini of the group of oligonucleotides which, after the attachment, form the repetitive unit of the gene. A synthetic gene of this type can be used to prepare recombinant plasmid vectors, to transform cells and thus to express peptides. The invention is applicable, inter alia, to the preparation of (asparagyl-phenylalanine)n and, from this, the sweetener aspartame, that is to say the methyl ester of asparagyl-phenylalanine. [FR2479259A1]
IE71/81A 1980-01-30 1981-01-15 Recombinant dna techniques IE50833B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8003191 1980-01-30

Publications (2)

Publication Number Publication Date
IE810071L true IE810071L (en) 1981-07-30
IE50833B1 IE50833B1 (en) 1986-07-23

Family

ID=10511011

Family Applications (1)

Application Number Title Priority Date Filing Date
IE71/81A IE50833B1 (en) 1980-01-30 1981-01-15 Recombinant dna techniques

Country Status (14)

Country Link
JP (1) JPS579795A (en)
AU (1) AU545908B2 (en)
BE (1) BE887290A (en)
CA (1) CA1189466A (en)
CH (1) CH662821A5 (en)
DE (1) DE3102721A1 (en)
DK (1) DK17881A (en)
ES (1) ES498948A0 (en)
FR (1) FR2479259B1 (en)
IE (1) IE50833B1 (en)
IL (1) IL61952A (en)
IT (1) IT1170653B (en)
NL (1) NL8100437A (en)
SE (1) SE451595B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU542264B2 (en) * 1979-06-01 1985-02-14 G.D. Searle & Co. Plasmid vectors
CA1213537A (en) * 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Polypeptide expression method
AU5762699A (en) * 1998-09-19 2000-04-10 Sang Jun Lee Dna cassette encoding a multimer of a biologically active peptide and a cleavable linker attached thereto and process for preparing the biologically active peptide

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0036258A3 (en) * 1980-03-14 1982-02-10 Cetus Corporation Process for producing aspartame

Also Published As

Publication number Publication date
FR2479259B1 (en) 1985-07-12
IT8147639A0 (en) 1981-01-27
SE451595B (en) 1987-10-19
NL8100437A (en) 1981-09-01
SE8100238L (en) 1981-07-31
IL61952A0 (en) 1981-02-27
JPS579795A (en) 1982-01-19
IL61952A (en) 1984-09-30
DK17881A (en) 1981-07-31
ES8203965A1 (en) 1982-04-16
IE50833B1 (en) 1986-07-23
BE887290A (en) 1981-05-14
AU6650781A (en) 1981-08-06
CH662821A5 (en) 1987-10-30
DE3102721C2 (en) 1988-11-10
IT1170653B (en) 1987-06-03
CA1189466A (en) 1985-06-25
FR2479259A1 (en) 1981-10-02
AU545908B2 (en) 1985-08-08
DE3102721A1 (en) 1982-01-07
ES498948A0 (en) 1982-04-16

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