IE821707L - Expression vectors - Google Patents

Expression vectors

Info

Publication number
IE821707L
IE821707L IE821707A IE170782A IE821707L IE 821707 L IE821707 L IE 821707L IE 821707 A IE821707 A IE 821707A IE 170782 A IE170782 A IE 170782A IE 821707 L IE821707 L IE 821707L
Authority
IE
Ireland
Prior art keywords
translation initiation
initiator codon
sequence
procaryotic
promoter
Prior art date
Application number
IE821707A
Other versions
IE53607B1 (en
Original Assignee
Genex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genex Corp filed Critical Genex Corp
Publication of IE821707L publication Critical patent/IE821707L/en
Publication of IE53607B1 publication Critical patent/IE53607B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

An expression vector for introducing a gene into a procaryotic organism, comprising a closed loop of DNA having the capability of replication in a procaryotic cell and including: (a) promoter, operator and translation initiation sequences, which are recognizable by the procaryotic host cell; (b) a Shine-Dalgarno sequence and an initiator codon within the translation initiation sequence the initiator codon being proximate to the end of the translation initiation sequence distal from the promoter and operator sequences; and (c) a recognition sequence for a restriction endonuclease, which is proximate to the initiator codon, and which, upon cleavage, leaves said initiator codon, or its complement, within the translation initiation sequence. r [GB2104901A]
IE1707/82A 1981-08-10 1982-07-15 Expression vectors IE53607B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29133481A 1981-08-10 1981-08-10

Publications (2)

Publication Number Publication Date
IE821707L true IE821707L (en) 1983-02-10
IE53607B1 IE53607B1 (en) 1988-12-21

Family

ID=23119888

Family Applications (1)

Application Number Title Priority Date Filing Date
IE1707/82A IE53607B1 (en) 1981-08-10 1982-07-15 Expression vectors

Country Status (10)

Country Link
JP (1) JPS5838300A (en)
AU (1) AU8592582A (en)
BE (1) BE893837A (en)
DE (1) DE3226515A1 (en)
FR (1) FR2511033B1 (en)
GB (1) GB2104901B (en)
IE (1) IE53607B1 (en)
IL (1) IL66263A (en)
IT (1) IT1156378B (en)
NL (1) NL8202851A (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4503142A (en) * 1982-06-25 1985-03-05 Litton Bionetics, Inc. Open reading frame vectors
EP0121569B1 (en) * 1982-10-08 1990-08-22 Kyowa Hakko Kogyo Co., Ltd. Novel vector
WO1984001583A1 (en) * 1982-10-08 1984-04-26 Kyowa Hakko Kogyo Kk Novel vector
JPS6054685A (en) * 1983-09-02 1985-03-29 Suntory Ltd Improved manifestation vector and its use
WO1985003522A1 (en) * 1984-02-08 1985-08-15 Cetus Corporation Monitoring and control systems for recombinant manipulations
JPH084507B2 (en) * 1985-10-03 1996-01-24 武田薬品工業株式会社 Novel DNA and polypeptide
FR2598430B1 (en) * 1986-05-06 1990-02-02 Roussel Uclaf NOVEL EXPRESSION VECTORS, PROCESS FOR OBTAINING SAME, AND APPLICATION THEREOF FOR EXPRESSING ANY PROTEIN IN ESCHERICHIA COLI
JPH04504950A (en) * 1988-11-23 1992-09-03 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Position-specific insertion vector and method using the same
NL9401678A (en) * 1994-10-12 1996-05-01 Evert Hovius Self-stabilizing loop method (Z.Z.S.L.).

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2450262A1 (en) * 1979-02-28 1980-09-26 Pasteur Institut Plasmid(s) contg. DNA fragment attached to operon - for transformation into host bacterium on yeast to produce nutrient proteins, esp. ovalbumin from E. coli (NL 11.12.79) albumin
SE8101665L (en) * 1980-03-17 1981-09-18 Harvard College POLYPEPTIDFRAMSTELLNING
EP0042246B1 (en) * 1980-06-12 1987-03-18 The Cancer Institute Of Japanese Foundation For Cancer Research Plasmid

Also Published As

Publication number Publication date
NL8202851A (en) 1983-03-01
IT8267895A0 (en) 1982-07-14
FR2511033A1 (en) 1983-02-11
FR2511033B1 (en) 1986-08-08
GB2104901A (en) 1983-03-16
GB2104901B (en) 1985-02-06
JPS5838300A (en) 1983-03-05
BE893837A (en) 1982-11-03
IT1156378B (en) 1987-02-04
IL66263A (en) 1985-12-31
AU8592582A (en) 1983-02-17
IE53607B1 (en) 1988-12-21
IL66263A0 (en) 1982-11-30
DE3226515A1 (en) 1983-02-17

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