IE930976A1 - A method for the expression of genes under the control of¹the lac operator - Google Patents

A method for the expression of genes under the control of¹the lac operator

Info

Publication number: IE930976A1
Authority: IE; Ireland
Prior art keywords: expression; galactose; gal; coli; growth
Prior art date: 1992-12-18

Application number

IE930976A

Other languages

English (en)

Inventor

Karl Bayer

Hermann Katinger

Walter Kramer

Christine Luttich

Diethard Mattanovich

Original Assignee

Polymun Scient Immunbio Forsch

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-12-18

Filing date

1993-12-17

Publication date

1994-06-29

1993-12-17 Application filed by Polymun Scient Immunbio Forsch filed Critical Polymun Scient Immunbio Forsch

1993-12-17 Priority to IE950847A priority Critical patent/IE71198B1/en

1994-06-29 Publication of IE930976A1 publication Critical patent/IE930976A1/en

Links

230000014509 gene expression Effects 0.000 title claims abstract description 38
238000000034 method Methods 0.000 title claims abstract description 34
108090000623 proteins and genes Proteins 0.000 title claims abstract description 33
229930182830 galactose Natural products 0.000 claims abstract description 46
241000588724 Escherichia coli Species 0.000 claims abstract description 26
102000007056 Recombinant Fusion Proteins Human genes 0.000 claims abstract description 20
108010008281 Recombinant Fusion Proteins Proteins 0.000 claims abstract description 20
230000012010 growth Effects 0.000 claims description 49
230000006698 induction Effects 0.000 claims description 32
239000002028 Biomass Substances 0.000 claims description 19
238000004519 manufacturing process Methods 0.000 claims description 11
239000000758 substrate Substances 0.000 claims description 11
230000015572 biosynthetic process Effects 0.000 claims description 10
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101150096316 5 gene Proteins 0.000 claims 1
230000001939 inductive effect Effects 0.000 abstract description 15
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238000001243 protein synthesis Methods 0.000 description 9
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239000013612 plasmid Substances 0.000 description 8
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 7
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DLRVVLDZNNYCBX-CAPXFGMSSA-N allolactose Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](O)O1 DLRVVLDZNNYCBX-CAPXFGMSSA-N 0.000 description 6
230000000694 effects Effects 0.000 description 6
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101710137500 T7 RNA polymerase Proteins 0.000 description 5
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238000011534 incubation Methods 0.000 description 3
239000006166 lysate Substances 0.000 description 3
238000010186 staining Methods 0.000 description 3
238000002965 ELISA Methods 0.000 description 2
241000701959 Escherichia virus Lambda Species 0.000 description 2
108010054278 Lac Repressors Proteins 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
229910019142 PO4 Inorganic materials 0.000 description 2
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
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108010034634 Repressor Proteins Proteins 0.000 description 2
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FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
102000019197 Superoxide Dismutase Human genes 0.000 description 2
108010012715 Superoxide dismutase Proteins 0.000 description 2
108010082433 UDP-glucose-hexose-1-phosphate uridylyltransferase Proteins 0.000 description 2
244000052616 bacterial pathogen Species 0.000 description 2
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235000013619 trace mineral Nutrition 0.000 description 2
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KDSPLKNONIUZSZ-HSUXUTPPSA-N (2r,4r,5r)-2,4,5,6-tetrahydroxyhexanal Chemical compound OC[C@@H](O)[C@H](O)C[C@@H](O)C=O KDSPLKNONIUZSZ-HSUXUTPPSA-N 0.000 description 1
VRYALKFFQXWPIH-HSUXUTPPSA-N 2-deoxy-D-galactose Chemical compound OC[C@@H](O)[C@H](O)[C@H](O)CC=O VRYALKFFQXWPIH-HSUXUTPPSA-N 0.000 description 1
WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 1
QRXMUCSWCMTJGU-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-N 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
208000027472 Galactosemias Diseases 0.000 description 1
101100454318 Lactobacillus casei lacT gene Proteins 0.000 description 1
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
239000005864 Sulphur Substances 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
JLQUFIHWVLZVTJ-UHFFFAOYSA-N carbosulfan Chemical compound CCCCN(CCCC)SN(C)C(=O)OC1=CC=CC2=C1OC(C)(C)C2 JLQUFIHWVLZVTJ-UHFFFAOYSA-N 0.000 description 1
230000030833 cell death Effects 0.000 description 1
230000032823 cell division Effects 0.000 description 1
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NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
238000012937 correction Methods 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
238000011161 development Methods 0.000 description 1
238000010586 diagram Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
238000010790 dilution Methods 0.000 description 1
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
235000019797 dipotassium phosphate Nutrition 0.000 description 1
229940079593 drug Drugs 0.000 description 1
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101150045500 galK gene Proteins 0.000 description 1
XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
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238000001727 in vivo Methods 0.000 description 1
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230000000977 initiatory effect Effects 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
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230000000670 limiting effect Effects 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
239000002207 metabolite Substances 0.000 description 1
239000000203 mixture Substances 0.000 description 1
239000000178 monomer Substances 0.000 description 1
229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
231100000219 mutagenic Toxicity 0.000 description 1
230000003505 mutagenic effect Effects 0.000 description 1
238000001426 native polyacrylamide gel electrophoresis Methods 0.000 description 1
230000003204 osmotic effect Effects 0.000 description 1
230000037361 pathway Effects 0.000 description 1
210000001322 periplasm Anatomy 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
230000001766 physiological effect Effects 0.000 description 1
239000002243 precursor Substances 0.000 description 1
238000005086 pumping Methods 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
230000002829 reductive effect Effects 0.000 description 1
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239000011780 sodium chloride Substances 0.000 description 1
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
150000008163 sugars Chemical class 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
230000029305 taxis Effects 0.000 description 1
238000012360 testing method Methods 0.000 description 1
238000006257 total synthesis reaction Methods 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
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238000012546 transfer Methods 0.000 description 1
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238000001262 western blot Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon

Landscapes

Genetics & Genomics (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

IE930976A 1992-12-18 1993-12-17 A method for the expression of genes under the control of¹the lac operator IE930976A1 (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
IE950847A IE71198B1 (en)	1992-12-18	1993-12-17	A method for the expression of genes under the control of the lac operator

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
AT251692A AT397812B (de)	1992-12-18	1992-12-18	Verfahren zur expression von genen unter kontrolle des lac-operators

Publications (1)

Publication Number	Publication Date
IE930976A1 true IE930976A1 (en)	1994-06-29

Family

ID=3535685

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IE930976A IE930976A1 (en)	1992-12-18	1993-12-17	A method for the expression of genes under the control of¹the lac operator

Country Status (2)

Country	Link
AT (1)	AT397812B (de)
IE (1)	IE930976A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7390645B2 (en)	1997-11-20	2008-06-24	William Marsh Rice University	Lactose repressor proteins with increased operator DNA binding affinity
AU1467399A (en) *	1997-11-20	1999-06-15	William Marsh Rice University	Lactose repressor proteins with altered ligand responsivity

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3925550A1 (de) *	1989-08-02	1991-02-07	Behringwerke Ag	Optimierte fermentationsverfahren zur herstellung von fremdproteinen in e.coli

1992
- 1992-12-18 AT AT251692A patent/AT397812B/de not_active IP Right Cessation
1993
- 1993-12-17 IE IE930976A patent/IE930976A1/en not_active IP Right Cessation

Also Published As

Publication number	Publication date
AT397812B (de)	1994-07-25
ATA251692A (de)	1993-11-15

Legal Events

Date	Code	Title	Description
1997-05-21	MM9A	Patent lapsed through non-payment of renewal fee

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