IL101142A - Shuttle vector plasmid replicable in the cells of escherichia coli and cyanobacteria - Google Patents

Shuttle vector plasmid replicable in the cells of escherichia coli and cyanobacteria

Info

Publication number: IL101142A
Authority: IL; Israel
Prior art keywords: plasmid; region; cells; escherichia coli; gene
Prior art date: 1991-03-08

Application number

IL101142A

Other languages

English (en)

Other versions

IL101142A0 (en

Original Assignee

Yoshihide Hagiware

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-03-08

Filing date

1992-03-04

Publication date

1998-06-15

1992-03-04 Application filed by Yoshihide Hagiware filed Critical Yoshihide Hagiware

1992-11-15 Publication of IL101142A0 publication Critical patent/IL101142A0/xx

1998-06-15 Publication of IL101142A publication Critical patent/IL101142A/en

Links

239000013612 plasmid Substances 0.000 title claims abstract description 112
241000588724 Escherichia coli Species 0.000 title claims abstract description 28
239000013605 shuttle vector Substances 0.000 title claims abstract description 20
241000192700 Cyanobacteria Species 0.000 title claims abstract description 15
108090000623 proteins and genes Proteins 0.000 claims abstract description 45
241000192589 Synechococcus elongatus PCC 7942 Species 0.000 claims abstract description 21
108010073254 Colicins Proteins 0.000 claims abstract description 3
239000012634 fragment Substances 0.000 claims description 36
108091008146 restriction endonucleases Proteins 0.000 claims description 19
241001464430 Cyanobacterium Species 0.000 claims description 5
239000013598 vector Substances 0.000 abstract description 10
108020004414 DNA Proteins 0.000 description 57
210000004027 cell Anatomy 0.000 description 26
239000000203 mixture Substances 0.000 description 22
238000011534 incubation Methods 0.000 description 16
238000000034 method Methods 0.000 description 13
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
238000006243 chemical reaction Methods 0.000 description 12
239000002609 medium Substances 0.000 description 12
229960000723 ampicillin Drugs 0.000 description 10
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 10
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 8
238000012869 ethanol precipitation Methods 0.000 description 8
230000010076 replication Effects 0.000 description 8
230000009466 transformation Effects 0.000 description 8
YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 7
238000010276 construction Methods 0.000 description 7
239000007788 liquid Substances 0.000 description 7
229920001817 Agar Polymers 0.000 description 6
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 6
239000008272 agar Substances 0.000 description 6
238000010367 cloning Methods 0.000 description 6
239000011780 sodium chloride Substances 0.000 description 6
102000002260 Alkaline Phosphatase Human genes 0.000 description 5
108020004774 Alkaline Phosphatase Proteins 0.000 description 5
230000000977 initiatory effect Effects 0.000 description 5
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
239000003513 alkali Substances 0.000 description 4
241000196324 Embryophyta Species 0.000 description 3
108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 3
239000006285 cell suspension Substances 0.000 description 3
230000002708 enhancing effect Effects 0.000 description 3
239000013613 expression plasmid Substances 0.000 description 3
229910001629 magnesium chloride Inorganic materials 0.000 description 3
239000003550 marker Substances 0.000 description 3
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
102000012410 DNA Ligases Human genes 0.000 description 2
108010061982 DNA Ligases Proteins 0.000 description 2
102000004218 Insulin-Like Growth Factor I Human genes 0.000 description 2
239000006142 Luria-Bertani Agar Substances 0.000 description 2
108010034634 Repressor Proteins Proteins 0.000 description 2
102000009661 Repressor Proteins Human genes 0.000 description 2
102000019197 Superoxide Dismutase Human genes 0.000 description 2
108010012715 Superoxide dismutase Proteins 0.000 description 2
241000192707 Synechococcus Species 0.000 description 2
239000011543 agarose gel Substances 0.000 description 2
238000000246 agarose gel electrophoresis Methods 0.000 description 2
XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
229910052782 aluminium Inorganic materials 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
239000003242 anti bacterial agent Substances 0.000 description 2
230000003115 biocidal effect Effects 0.000 description 2
229940041514 candida albicans extract Drugs 0.000 description 2
239000013599 cloning vector Substances 0.000 description 2
239000011888 foil Substances 0.000 description 2
230000002068 genetic effect Effects 0.000 description 2
238000002955 isolation Methods 0.000 description 2
239000004325 lysozyme Substances 0.000 description 2
229960000274 lysozyme Drugs 0.000 description 2
230000000813 microbial effect Effects 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
108090000765 processed proteins & peptides Proteins 0.000 description 2
230000001681 protective effect Effects 0.000 description 2
102000004169 proteins and genes Human genes 0.000 description 2
238000003756 stirring Methods 0.000 description 2
239000012138 yeast extract Substances 0.000 description 2
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
229920000936 Agarose Polymers 0.000 description 1
239000004382 Amylase Substances 0.000 description 1
241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
102000003846 Carbonic anhydrases Human genes 0.000 description 1
108090000209 Carbonic anhydrases Proteins 0.000 description 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
206010059866 Drug resistance Diseases 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
241001131785 Escherichia coli HB101 Species 0.000 description 1
101100383220 Escherichia coli col gene Proteins 0.000 description 1
241000701959 Escherichia virus Lambda Species 0.000 description 1
108700039887 Essential Genes Proteins 0.000 description 1
VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
239000005977 Ethylene Substances 0.000 description 1
241000193386 Lysinibacillus sphaericus Species 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
108700001094 Plant Genes Proteins 0.000 description 1
241000206572 Rhodophyta Species 0.000 description 1
102000013275 Somatomedins Human genes 0.000 description 1
239000002585 base Substances 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
239000001569 carbon dioxide Substances 0.000 description 1
229910002092 carbon dioxide Inorganic materials 0.000 description 1
210000005056 cell body Anatomy 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
229960005091 chloramphenicol Drugs 0.000 description 1
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
230000029087 digestion Effects 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
239000000499 gel Substances 0.000 description 1
-1 i.e. Substances 0.000 description 1
239000005457 ice water Substances 0.000 description 1
230000000749 insecticidal effect Effects 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
235000021317 phosphate Nutrition 0.000 description 1
150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
230000000243 photosynthetic effect Effects 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
230000002062 proliferating effect Effects 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000000926 separation method Methods 0.000 description 1
AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
235000019345 sodium thiosulphate Nutrition 0.000 description 1
239000000126 substance Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000000844 transformation Methods 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

Landscapes

Genetics & Genomics (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Saccharide Compounds (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

IL101142A 1991-03-08 1992-03-04 Shuttle vector plasmid replicable in the cells of escherichia coli and cyanobacteria IL101142A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP3067774A JP2574549B2 (ja)	1991-03-08	1991-03-08	新規プラスミド

Publications (2)

Publication Number	Publication Date
IL101142A0 IL101142A0 (en)	1992-11-15
IL101142A true IL101142A (en)	1998-06-15

Family

ID=13354631

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL101142A IL101142A (en)	1991-03-08	1992-03-04	Shuttle vector plasmid replicable in the cells of escherichia coli and cyanobacteria

Country Status (15)

Country	Link
EP (1)	EP0576674B1 (de)
JP (1)	JP2574549B2 (de)
KR (1)	KR100213526B1 (de)
AT (1)	ATE181109T1 (de)
AU (1)	AU638892B2 (de)
CA (1)	CA2082407A1 (de)
DE (1)	DE69229392T2 (de)
DK (1)	DK0576674T3 (de)
ES (1)	ES2134209T3 (de)
FI (1)	FI107736B (de)
HU (1)	HU216075B (de)
IL (1)	IL101142A (de)
RU (1)	RU2122027C1 (de)
TW (1)	TW213951B (de)
WO (1)	WO1992015692A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH06253820A (ja) *	1993-02-26	1994-09-13	Hideo Fukuda	細菌のエチレン生成酵素をコードする遺伝子を含むｄｎａ断片を含有するベクターで形質転換させたらん藻の形質転換体と、その形質転換体を利用したエチレン製造方法
JPH10150984A (ja) *	1996-11-19	1998-06-09	Hagiwara Yoshihide	抗体分子の製造方法
RU2286383C2 (ru) *	2001-07-30	2006-10-27	Хабаровский научно-исследовательский институт эпидемиологии и микробиологии	Штамм бактерий shigella sonnei, используемый в качестве индикатора для типирования колицинов группы е3 (е3, е4, е5, е6) у энтеробактерий
RU2360961C1 (ru) *	2007-10-17	2009-07-10	Федеральное государственное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФГУН ГНЦ ВБ "Вектор" Роспотребнадзора)	Способ получения тонкодисперсной культуры клеток микобактерий, чувствительной к инфицированию микобактериофагами
RU2485384C2 (ru) *	2011-09-01	2013-06-20	Вадим Андреевич Иванов	Фронтально-козловой трубоукладчик

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ES2029785T3 (es) *	1985-10-21	1992-10-01	Fujisawa Pharmaceutical Co., Ltd.	Procedimiento para producir factor de crecimiento tipo insulina i y plasmido para su produccion.
JPS63294788A (ja) *	1987-05-27	1988-12-01	Nikka Uisukii Kk	ベクタ−、遺伝子の検出方法及び蛋白質の発現方法
JPH0284187A (ja) *	1988-09-20	1990-03-26	Agency Of Ind Science & Technol	シャトルベクターＹＥＵｐ３及び菌体内にＹＥＵｐ３を保持する微生物菌株
JPH0767393B2 (ja) *	1991-03-13	1995-07-26	萩原義秀	ポリペプチドの発現方法

1991
- 1991-03-08 JP JP3067774A patent/JP2574549B2/ja not_active Expired - Fee Related
1992
- 1992-03-04 IL IL101142A patent/IL101142A/en not_active IP Right Cessation
- 1992-03-06 EP EP92905782A patent/EP0576674B1/de not_active Expired - Lifetime
- 1992-03-06 AT AT92905782T patent/ATE181109T1/de not_active IP Right Cessation
- 1992-03-06 AU AU13611/92A patent/AU638892B2/en not_active Ceased
- 1992-03-06 RU RU92016430A patent/RU2122027C1/ru not_active IP Right Cessation
- 1992-03-06 CA CA002082407A patent/CA2082407A1/en not_active Abandoned
- 1992-03-06 DE DE69229392T patent/DE69229392T2/de not_active Expired - Fee Related
- 1992-03-06 WO PCT/JP1992/000267 patent/WO1992015692A1/ja not_active Ceased
- 1992-03-06 TW TW081101714A patent/TW213951B/zh active
- 1992-03-06 KR KR1019920702777A patent/KR100213526B1/ko not_active Expired - Fee Related
- 1992-03-06 ES ES92905782T patent/ES2134209T3/es not_active Expired - Lifetime
- 1992-03-06 DK DK92905782T patent/DK0576674T3/da active
- 1992-03-06 HU HUP9203485A patent/HU216075B/hu not_active IP Right Cessation
- 1992-11-05 FI FI925004A patent/FI107736B/fi not_active IP Right Cessation

Also Published As

Publication number	Publication date
FI107736B (fi)	2001-09-28
HUT65745A (en)	1994-07-28
HU9203485D0 (en)	1993-01-28
AU1361192A (en)	1992-10-06
WO1992015692A1 (en)	1992-09-17
RU2122027C1 (ru)	1998-11-20
IL101142A0 (en)	1992-11-15
EP0576674A1 (de)	1994-01-05
HU216075B (hu)	1999-04-28
JPH04281793A (ja)	1992-10-07
EP0576674B1 (de)	1999-06-09
ES2134209T3 (es)	1999-10-01
KR100213526B1 (ko)	1999-08-02
AU638892B2 (en)	1993-07-08
TW213951B (de)	1993-10-01
ATE181109T1 (de)	1999-06-15
FI925004L (fi)	1992-11-05
FI925004A0 (fi)	1992-11-05
EP0576674A4 (de)	1994-04-27
DE69229392D1 (de)	1999-07-15
JP2574549B2 (ja)	1997-01-22
DK0576674T3 (da)	1999-11-15
CA2082407A1 (en)	1992-09-09
DE69229392T2 (de)	1999-10-07

Legal Events

Date	Code	Title
1998-11-26	FF	Patent granted
1999-04-11	KB	Patent renewed
2006-12-07	MM9K	Patent not in force due to non-payment of renewal fees

Publication	Publication Date	Title
Kahn et al.	1979	[17] Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2
Helmer et al.	1984	A new chimeric gene as a marker for plant transformation: the expression of Escherichia coli β-galactosidase in sunflower and tobacco cells
Genilloud et al.	1984	The transposon Tn5 carries a bleomycin-resistance determinant
EP0131623B2 (de)	1999-07-28	Chimärische gene geeignet zur expression in pflanzenzellen
Sherman et al.	1982	Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans
US10000761B2 (en)	2018-06-19	DNA molecule used for recombinant Pichia plasmid and recombinant Pichia strain expressing PprI protein of Deinococcus radiodurans
AU6867598A (en)	1998-10-20	(chlorella) virus promoters
JPH10503090A (ja)	1998-03-24	低温での組換え蛋白質産生のためのベクターおよび形質転換宿主細胞
IL101142A (en)	1998-06-15	Shuttle vector plasmid replicable in the cells of escherichia coli and cyanobacteria
Toh-e et al.	1980	A stable plasmid carrying the yeast Leu2 gene and containing only yeast deoxyribonucleic acid
CN105671061A (zh)	2016-06-15	植物乳杆菌素plnE和plnF的异源表达方法
Blackhart et al.	1986	Analysis of the products of the Myxococcus xanthus frz genes
US5821119A (en)	1998-10-13	Shuttle vectors for Escherichia coli and cyanobateria
Laudenbach et al.	1983	The large endogenous plasmid of Anacystis nidulans: Mapping, cloning and localization of the origin of replication
CN115819626B (zh)	2026-04-28	重组铁蛋白纳米颗粒及其制备方法
NL8503316A (nl)	1987-06-16	Werkwijze voor het bereiden van proteinen met behulp van gastheer-organismen, in het bijzonder melkzuurbacterien, welke recombinant dna-plasmiden met een hoge replicon-aktiviteit en een hoge promotor-aktiviteit voor het betreffende proteine-coderende gen bevatten, de van dergelijke recombinant dna-plasmiden voorziene gastheerorganismen, de recombinant dna-plasmiden zelve, de replicon-aktiviteit en promotor-aktiviteit coderende dna-fragmenten, alsmede de verkregen proteinen.
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Olins et al.	1983	Overproduction of Escherichia coli NusA protein
Takeshima et al.	1994	A novel expression vector for the cyanobacterium, Synechococcus PCC 6301
Lin et al.	1985	Mutations that affect the translation efficiency of Tn9-derived cat gene in Bacillus subtilis.
Lowman et al.	1988	High-level expression of the simian virus 40 genes LP1, VP1 and VP2 as fusion proteins in Escherichia coli
CN114908111A (zh)	2022-08-16	连续克隆长dna片段的方法和系统
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