IL114564A - Method for the sensitive detection of nucleic acids - Google Patents

Method for the sensitive detection of nucleic acids

Info

Publication number
IL114564A
IL114564A IL11456495A IL11456495A IL114564A IL 114564 A IL114564 A IL 114564A IL 11456495 A IL11456495 A IL 11456495A IL 11456495 A IL11456495 A IL 11456495A IL 114564 A IL114564 A IL 114564A
Authority
IL
Israel
Prior art keywords
nucleic acid
sensitive detection
nucleic acids
hybridize
target nucleic
Prior art date
Application number
IL11456495A
Other languages
English (en)
Other versions
IL114564A0 (en
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4431269A external-priority patent/DE4431269A1/de
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of IL114564A0 publication Critical patent/IL114564A0/xx
Publication of IL114564A publication Critical patent/IL114564A/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
IL11456495A 1994-07-16 1995-07-12 Method for the sensitive detection of nucleic acids IL114564A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4425264 1994-07-16
DE4431269A DE4431269A1 (de) 1994-07-16 1994-09-02 Verfahren zum sensitiven Nachweis von Nukleinsäuren

Publications (2)

Publication Number Publication Date
IL114564A0 IL114564A0 (en) 1995-11-27
IL114564A true IL114564A (en) 1999-11-30

Family

ID=25938437

Family Applications (1)

Application Number Title Priority Date Filing Date
IL11456495A IL114564A (en) 1994-07-16 1995-07-12 Method for the sensitive detection of nucleic acids

Country Status (10)

Country Link
US (1) US5830664A (fr)
EP (1) EP0705905B1 (fr)
JP (1) JP3360977B2 (fr)
KR (1) KR0161327B1 (fr)
CN (1) CN1073160C (fr)
AT (1) ATE206764T1 (fr)
CA (1) CA2153944C (fr)
ES (1) ES2165402T3 (fr)
FI (1) FI114716B (fr)
IL (1) IL114564A (fr)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US6872816B1 (en) * 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US5994069A (en) * 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6153425A (en) * 1995-07-13 2000-11-28 Xtrana, Inc. Self-contained device integrating nucleic acid extraction, amplification and detection
US7122364B1 (en) * 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US6913881B1 (en) 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US7432048B2 (en) * 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US5985557A (en) * 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US20080160524A1 (en) * 1996-01-24 2008-07-03 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
US7195871B2 (en) * 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US7527928B2 (en) * 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
CA2273204C (fr) 1996-11-29 2008-10-14 Third Wave Technologies, Inc. Agents de clivage ameliores
JP4502502B2 (ja) * 1997-10-28 2010-07-14 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア フローサイトメトリーを用いるｄｎａ多型の同定方法
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
WO2002070755A2 (fr) * 2000-11-15 2002-09-12 Third Wave Technologies, Inc. Endonucleases fen
EP1367136A4 (fr) * 2001-02-15 2005-01-12 Takara Bio Inc Procede de detection de polymorphisme de nucleotide
US6596489B2 (en) * 2001-03-30 2003-07-22 Applied Gene Technologies Methods and compositions for analyzing nucleotide sequence mismatches using RNase H
US20030165935A1 (en) * 2001-11-21 2003-09-04 Vann Charles S. Digital assay
AU2003261970A1 (en) * 2002-09-06 2004-03-29 Wakunaga Pharmaceutical Co., Ltd. Method of identifying nucleic acid having polymorphism sequence site
DE602004029694D1 (de) * 2003-08-11 2010-12-02 Univ Georgia State Res Found Rna-nachweis und quantifizierung
KR100652903B1 (ko) * 2005-12-21 2006-12-04 한국과학기술연구원 초흡수성 고분자를 함유한 제습제의 제조 방법 및 그 제조장치
WO2009117327A2 (fr) * 2008-03-15 2009-09-24 Hologic, Inc. Compositions et procédés pour analyse de molécules d’acide nucléique pendant des réactions d’amplification
EP2644708B1 (fr) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. Dosages à base de rnase-h utilisant des monomères d'arn modifiés
US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
CN107739750A (zh) * 2009-09-25 2018-02-27 艾利尔圣迭戈公司 粗基质中的核酸的检测
US20110117559A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
WO2012135053A2 (fr) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Essais à base d'arnase h faisant appel à des monomères d'arn modifiés
CA2906365A1 (fr) * 2013-03-15 2014-09-18 Integrated Dna Technologies, Inc. Essais a base d'arnase-h utilisant de monomeres d'arn modifies
EP3643790A4 (fr) * 2017-06-23 2021-03-24 Eiken Kagaku Kabushiki Kaisha Méthode de détection d'acide nucléique, amorce pour la détection d'acide nucléique, et kit pour la détection d'acide nucléique
JP7333171B2 (ja) * 2018-12-18 2023-08-24 栄研化学株式会社 Ｒｎａ検出方法、ｒｎａ検出用核酸及びｒｎａ検出用キット
KR102261979B1 (ko) * 2019-10-28 2021-06-07 재단법인 대구경북첨단의료산업진흥재단 핵산분해효소 연쇄 반응

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
JP2856804B2 (ja) * 1988-03-24 1999-02-10 ユニバーシティ オブ アイオワ リサーチ ファウンデイション 標識された相補的核酸プローブを開裂する触媒反応の補因子としての活性に基づく核酸配列検出のための触媒ハイブリッド形成系
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5102784A (en) * 1990-05-04 1992-04-07 Oncor, Inc. Restriction amplification assay
WO1993005184A1 (fr) * 1991-09-10 1993-03-18 Love Jack D Amplification de cibles et de signaux d'adn/arn
JP3403405B2 (ja) * 1991-09-13 2003-05-06 サイトセル・リミテッド 核酸伸長検定
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US5518901A (en) * 1993-04-19 1996-05-21 Murtagh; James J. Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease
DE4441626A1 (de) * 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren

Also Published As

Publication number Publication date
CA2153944A1 (fr) 1996-01-17
FI953445A0 (fi) 1995-07-14
CN1073160C (zh) 2001-10-17
IL114564A0 (en) 1995-11-27
EP0705905A3 (fr) 1999-03-03
EP0705905B1 (fr) 2001-10-10
JPH0856700A (ja) 1996-03-05
EP0705905A2 (fr) 1996-04-10
US5830664A (en) 1998-11-03
KR0161327B1 (ko) 1998-11-16
ES2165402T3 (es) 2002-03-16
CN1124294A (zh) 1996-06-12
FI114716B (fi) 2004-12-15
JP3360977B2 (ja) 2003-01-07
FI953445L (fi) 1996-01-17
ATE206764T1 (de) 2001-10-15
CA2153944C (fr) 2002-12-24

Similar Documents

Publication Publication Date Title
IL114564A0 (en) Method for the sensitive detection of nucleic acids
CA2230967A1 (fr) Dosages ameliores des acides nucleiques
AU548854B2 (en) A method and reagent combination for the diagnosis of microorganisms
EP0281927A3 (fr) Procédé pour déterminer une sequence d'acide nucléique dans un échantillon
PL307341A1 (en) Method of as well as reagent and kit for detecting and amplifying a sequence of nucleic acids
AU4902590A (en) Assay method
ES2006377A6 (es) Un metodo de analisis de acidos nucleicos por hibridacion.
EP0714987A3 (fr) Procédé pour quantifier d'ADN génomiques
EP0709466A3 (fr)
CA2145576A1 (fr) Detection de l'amplification de l'acide nucleique
WO1996020287A3 (fr) Detection d'acides nucleiques par formation d'un produit catalyse par une cible
WO1996006190A3 (fr) Procede de ligature et d'amplification associees
CA2163388A1 (fr) Methodes pour l'extinction en solution d'oligonucleotides marques par fluorescence
MXPA03001879A (es) Metodo y sistema para detectar acidos nucleicos.
AU7517496A (en) Methods for preparing solid supports for hybridization and reducing non-specific background
AU1272000A (en) Method for the quantitative detection of nucleic acids
WO1997007235A3 (fr) Methode integrale d'amplification d'acide nucleique
CA2295942A1 (fr) Amelioration de la specificite de l'amplification d'acides nucleiques par un acide nucleique porteur
EP0128018A3 (en) Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
CA2219458A1 (fr) Detection d'une sequence nucleotidique avec amplification de signal
WO2001029262A3 (fr) Reactifs de genotypage, kits et procedes d'utilisation desdits reactifs
EP0235727A3 (fr) DNA-diagnostique rapide pour les microbes
WO1997043446A3 (fr) Methode et appareil permettant de diagnostiquer une predisposition a certaines affections, notamment l'osteoporose, sur la base du polymorphisme d'un gene de l'il-6
CA2140331A1 (fr) Detection et amplification d'acides nucleiques cibles par l'activite exonucleolytique
CA2213731A1 (fr) Procede et reactifs de typage de genes hla de classe i

Legal Events

Date Code Title Description
FF Patent granted
KB Patent renewed
KB Patent renewed
KB Patent renewed
KB Patent renewed
MM9K Patent not in force due to non-payment of renewal fees