IL124322A - Detection of an entity in a sample - Google Patents

Detection of an entity in a sample

Info

Publication number: IL124322A
Authority: IL; Israel
Prior art keywords: electrodes; assay; target; bridge; sample
Prior art date: 1998-05-04

Application number

IL12432298A

Other languages

English (en)

Other versions

IL124322A0 (en

Inventor

Yoav Eichen

Uri Sivan

Erez Braun

Original Assignee

Technion Res & Dev Foundation

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-05-04

Filing date

1998-05-04

Publication date

2002-05-23

1998-05-04 Application filed by Technion Res & Dev Foundation filed Critical Technion Res & Dev Foundation

1998-05-04 Priority to IL12432298A priority Critical patent/IL124322A/en

1998-12-06 Publication of IL124322A0 publication Critical patent/IL124322A0/xx

1999-05-04 Priority to JP2000547465A priority patent/JP4618886B2/ja

1999-05-04 Priority to CA2820735A priority patent/CA2820735A1/fr

1999-05-04 Priority to AU36259/99A priority patent/AU3625999A/en

1999-05-04 Priority to CA2641613A priority patent/CA2641613C/fr

1999-05-04 Priority to CA002331688A priority patent/CA2331688C/fr

1999-05-04 Priority to PCT/IL1999/000232 priority patent/WO1999057550A1/fr

1999-05-04 Priority to AT99918252T priority patent/ATE515693T1/de

1999-05-04 Priority to EP99918252A priority patent/EP1075656B1/fr

2002-05-23 Publication of IL124322A publication Critical patent/IL124322A/en

2003-06-03 Priority to US10/452,139 priority patent/US20030203394A1/en

2011-08-03 Priority to US13/197,294 priority patent/US20120309106A1/en

2016-08-08 Priority to US15/231,264 priority patent/US20170121761A1/en

Links

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239000002336 ribonucleotide Substances 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
150000003384 small molecules Chemical class 0.000 description 1
239000007787 solid Substances 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
241000894007 species Species 0.000 description 1
230000002269 spontaneous effect Effects 0.000 description 1
230000006641 stabilisation Effects 0.000 description 1
238000011105 stabilization Methods 0.000 description 1
235000011149 sulphuric acid Nutrition 0.000 description 1
239000000725 suspension Substances 0.000 description 1
229920001059 synthetic polymer Polymers 0.000 description 1
RAOIDOHSFRTOEL-UHFFFAOYSA-N tetrahydrothiophene Chemical group C1CCSC1 RAOIDOHSFRTOEL-UHFFFAOYSA-N 0.000 description 1
238000007669 thermal treatment Methods 0.000 description 1
DTMHTVJOHYTUHE-UHFFFAOYSA-N thiocyanogen Chemical compound N#CSSC#N DTMHTVJOHYTUHE-UHFFFAOYSA-N 0.000 description 1
150000003573 thiols Chemical class 0.000 description 1
RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1
RCHUVCPBWWSUMC-UHFFFAOYSA-N trichloro(octyl)silane Chemical compound CCCCCCCC[Si](Cl)(Cl)Cl RCHUVCPBWWSUMC-UHFFFAOYSA-N 0.000 description 1
ZDHXKXAHOVTTAH-UHFFFAOYSA-N trichlorosilane Chemical compound Cl[SiH](Cl)Cl ZDHXKXAHOVTTAH-UHFFFAOYSA-N 0.000 description 1
239000005052 trichlorosilane Substances 0.000 description 1
229920003169 water-soluble polymer Polymers 0.000 description 1
238000009736 wetting Methods 0.000 description 1
239000001018 xanthene dye Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Immunology (AREA)
Zoology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Wood Science & Technology (AREA)
Molecular Biology (AREA)
Analytical Chemistry (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Biophysics (AREA)
Genetics & Genomics (AREA)
General Engineering & Computer Science (AREA)
Urology & Nephrology (AREA)
Biomedical Technology (AREA)
Hematology (AREA)
Medicinal Chemistry (AREA)
Food Science & Technology (AREA)
Cell Biology (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Analysing Materials By The Use Of Radiation (AREA)
Investigating Or Analysing Biological Materials (AREA)
Detergent Compositions (AREA)
Ultra Sonic Daignosis Equipment (AREA)

IL12432298A 1998-05-04 1998-05-04 Detection of an entity in a sample IL124322A (en)

Priority Applications (12)

Application Number	Priority Date	Filing Date	Title
IL12432298A IL124322A (en)	1998-05-04	1998-05-04	Detection of an entity in a sample
EP99918252A EP1075656B1 (fr)	1998-05-04	1999-05-04	Detection d'une cible dans un echantillon
CA2641613A CA2641613C (fr)	1998-05-04	1999-05-04	Detection d'une cible dans un echantillon
CA2820735A CA2820735A1 (fr)	1998-05-04	1999-05-04	Detection d'une cible dans un echantillon
AU36259/99A AU3625999A (en)	1998-05-04	1999-05-04	Detection of a target in a sample
JP2000547465A JP4618886B2 (ja)	1998-05-04	1999-05-04	サンプル中の標的の検出
CA002331688A CA2331688C (fr)	1998-05-04	1999-05-04	Detection d'une cible dans un echantillon
PCT/IL1999/000232 WO1999057550A1 (fr)	1998-05-04	1999-05-04	Detection d'une cible dans un echantillon
AT99918252T ATE515693T1 (de)	1998-05-04	1999-05-04	Nachweis einer zielsubstanz in einer probe
US10/452,139 US20030203394A1 (en)	1998-05-04	2003-06-03	Detection of a target in a sample
US13/197,294 US20120309106A1 (en)	1998-05-04	2011-08-03	Detection of a target in a sample
US15/231,264 US20170121761A1 (en)	1998-05-04	2016-08-08	Electrical detection of a dna or rna target in a sample

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
IL12432298A IL124322A (en)	1998-05-04	1998-05-04	Detection of an entity in a sample

Publications (2)

Publication Number	Publication Date
IL124322A0 IL124322A0 (en)	1998-12-06
IL124322A true IL124322A (en)	2002-05-23

Family

ID=11071451

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL12432298A IL124322A (en)	1998-05-04	1998-05-04	Detection of an entity in a sample

Country Status (8)

Country	Link
US (2)	US20120309106A1 (fr)
EP (1)	EP1075656B1 (fr)
JP (1)	JP4618886B2 (fr)
AT (1)	ATE515693T1 (fr)
AU (1)	AU3625999A (fr)
CA (3)	CA2641613C (fr)
IL (1)	IL124322A (fr)
WO (1)	WO1999057550A1 (fr)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL121312A (en)	1997-07-14	2001-09-13	Technion Res & Dev Foundation	Microelectronic components, their manufacture and electronic networks containing them
IL126776A (en)	1998-10-27	2001-04-30	Technion Res & Dev Foundation	A method of investing gold
US6399303B1 (en)	1999-04-07	2002-06-04	Integrated Nano-Technologies, Llc	High resolution DNA detection methods and devices
US7364920B2 (en)	1999-10-27	2008-04-29	Technion Research And Development Foundation Ltd.	Method for gold deposition
DE10113550A1 (de) *	2001-03-20	2002-10-02	Infineon Technologies Ag	Verfahren zum Erfassen von makromolekularen Biopolymeren mittels einer Elektrodenanordnung
US6824974B2 (en)	2001-06-11	2004-11-30	Genorx, Inc.	Electronic detection of biological molecules using thin layers
EP1417352A4 (fr) *	2001-06-11	2006-11-08	Genorx Inc	Detection electronique de molecules biologiques au moyen de fines couches
US20040023253A1 (en) *	2001-06-11	2004-02-05	Sandeep Kunwar	Device structure for closely spaced electrodes
CN100344960C (zh) *	2001-12-17	2007-10-24	清华大学	刺激动物细胞并记录其生理信号的装置及其生产使用方法
US20030186245A1 (en) *	2002-03-28	2003-10-02	Roitman Daniel B.	Biomolecular sensors and detection methods utilizing photoinduced charge separation
DE10228260A1 (de) *	2002-06-25	2004-01-22	Bayer Ag	Methode und Vorrichtung zum impedimetrischen Nachweis eines oder mehrerer Analyten in einer Probe
US20040166520A1 (en) *	2003-01-03	2004-08-26	Connolly D. Michael	Identifying items with nucleic acid taggants
DE10322912A1 (de) *	2003-05-21	2004-12-16	Bayer Technology Services Gmbh	Verfahren zum Nachweis von Nukleinsäuren
DE10327683A1 (de) *	2003-06-20	2005-01-20	Bayer Technology Services Gmbh	Verfahren und Vorrichtung zum quantitativen elektrischen Nachweis von Analyten
DE10330717A1 (de) *	2003-07-03	2005-02-10	Friedrich-Schiller-Universität Jena	Vorrichtung und Verfahren zum Nachweis von Analyt
US20050079598A1 (en) *	2003-10-08	2005-04-14	Davis Randall W.	Apparatus and method for identification of biomolecules, in particular nucleic acid sequences, proteins, and antigens and antibodies
JP4389088B2 (ja) *	2003-11-07	2009-12-24	公立大学法人大阪府立大学	電気抵抗型検出センサおよび検出方法
WO2005111619A1 (fr) *	2004-04-29	2005-11-24	Marc Ramael	Procédé et kit pour détecter des composants dans un échantillon
WO2005118870A2 (fr) *	2004-05-28	2005-12-15	Nanogen, Inc.	Systemes de detection electronique nanometriques et leurs procede de production
JP4873463B2 (ja) *	2006-06-30	2012-02-08	独立行政法人産業技術総合研究所	ナノギャップ電極を有する検出素子及びこれを用いた検出方法
WO2008124706A2 (fr)	2007-04-06	2008-10-16	Arizona Board Of Regents Acting For And On Behalf Of Arizona State University	Dispositifs et procédés pour une caractérisation de molécule cible
WO2010042514A1 (fr)	2008-10-06	2010-04-15	Arizona Board Of Regents	Séquenceur d'adn basé sur des nanopores et des nanotubes de carbone et éléments de reconnaissance sériels
EP2517028B1 (fr)	2010-02-02	2017-12-13	Arizona Board Of Regents	Dispositif à écartement tunnel régulé pour le séquençage de polymères
EP2906720A4 (fr)	2012-10-10	2016-06-01	Univ Arizona	Systèmes et dispositifs pour détecter des molécules et leur procédé de fabrication
CA3050062A1 (fr)	2016-01-14	2017-07-20	Roswell Biotechnologies, Inc.	Capteurs moleculaires et procedes associes
JP7280590B2 (ja) *	2016-01-28	2023-05-24	ロズウェルバイオテクノロジーズ，インコーポレイテッド	大スケールの分子電子工学センサアレイを使用する被分析物を測定するための方法および装置
EP3408219B1 (fr)	2016-01-28	2022-08-17	Roswell Biotechnologies, Inc	Appareil de séquençage d'adn massivement parallèle
US10737263B2 (en)	2016-02-09	2020-08-11	Roswell Biotechnologies, Inc.	Electronic label-free DNA and genome sequencing
EP3458613B1 (fr) *	2016-05-18	2022-09-14	Integrated Nano-Technologies, Inc.	Procédé de détection d'un produit de pcr
CN110520517A (zh)	2017-01-19	2019-11-29	罗斯威尔生命技术公司	包括二维层材料的固态测序装置
KR102879682B1 (ko)	2017-04-25	2025-10-30	로스웰 엠이 아이엔씨.	분자 센서들을 위한 효소 회로들
US10508296B2 (en)	2017-04-25	2019-12-17	Roswell Biotechnologies, Inc.	Enzymatic circuits for molecular sensors
CA3057155A1 (fr)	2017-05-09	2018-11-15	Roswell Biotechnologies, Inc.	Circuits de sonde de liaison pour capteurs moleculaires
EP3676389A4 (fr)	2017-08-30	2021-06-02	Roswell Biotechnologies, Inc	Capteurs électroniques moléculaires à enzyme processive pour le stockage de données d'adn
EP3694990A4 (fr)	2017-10-10	2022-06-15	Roswell Biotechnologies, Inc.	Procédés, appareil et systèmes pour le stockage de données d'adn sans amplification
US20210373014A1 (en) *	2017-10-23	2021-12-02	The General Hospital Corporation	An integrated microfluidic electrode array system for enzyme-linked immuno-sorbent assay (easy-elisa) for point-of-care detection of molecular and cellular biomarkers
AU2019269615B2 (en)	2018-05-17	2023-04-06	Recognition AnalytiX, Inc.	Device, system and method for direct electrical measurement of enzyme activity
JP2020041909A (ja) *	2018-09-11	2020-03-19	国立大学法人九州大学	内分泌攪乱物質等の定量方法及び定量装置
US12351855B2 (en)	2019-01-30	2025-07-08	Arizona Board Of Regents On Behalf Of Arizona State University	Bioelectronic circuits, systems and methods for preparing and using them
CN113994013A (zh)	2019-04-12	2022-01-28	罗斯威尔生命技术公司	用于分子电子学传感器的多环芳族桥
JP2022544663A (ja) *	2019-08-22	2022-10-20	ユニバーサルシーケンシングテクノロジーコーポレイション	生体分子の検知および検出のための方法
WO2021045900A1 (fr)	2019-09-06	2021-03-11	Roswell Biotechnologies, Inc.	Procédés de fabrication de structures à l'échelle nanométrique utilisables dans des capteurs moléculaires et d'autres dispositifs
IL297732A (en) *	2020-04-30	2022-12-01	Univ Arizona State	Methods for sequencing biopolymers
US12385873B2 (en)	2020-12-25	2025-08-12	Aurangzeb Nafees Nagy	Detection of target nucleic acid molecules
JP7555166B1 (ja) *	2024-07-04	2024-09-24	株式会社イムノセンス	電気化学式免疫センサー

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5137827A (en) *	1986-03-25	1992-08-11	Midwest Research Technologies, Inc.	Diagnostic element for electrical detection of a binding reaction
US4794089A (en) *	1986-03-25	1988-12-27	Midwest Research Microscopy, Inc.	Method for electronic detection of a binding reaction
US5108889A (en) *	1988-10-12	1992-04-28	Thorne, Smith, Astill Technologies, Inc.	Assay for determining analyte using mercury release followed by detection via interaction with aluminum
AU4647589A (en) *	1988-11-10	1990-05-28	Midwest Research Technologies, Inc.	Method for electrical detection of a binding reaction
CA2034266A1 (fr) *	1990-02-27	1991-08-28	Paul A. D'orazio	Couche d'epandage et d'immobilisation des reactifs en pellicule epaisse
US5846708A (en) *	1991-11-19	1998-12-08	Massachusetts Institiute Of Technology	Optical and electrical methods and apparatus for molecule detection
IL103674A0 (en) *	1991-11-19	1993-04-04	Houston Advanced Res Center	Method and apparatus for molecule detection
WO1997021094A1 (fr) *	1995-12-01	1997-06-12	Innogenetics N.V.	Systeme de detection par mesure de l'impedance et procede pour le fabriquer
US6506895B2 (en) *	1997-08-15	2003-01-14	Surmodics, Inc.	Photoactivatable nucleic acids
IL126776A (en) *	1998-10-27	2001-04-30	Technion Res & Dev Foundation	A method of investing gold

1998
- 1998-05-04 IL IL12432298A patent/IL124322A/en not_active IP Right Cessation
1999
- 1999-05-04 AT AT99918252T patent/ATE515693T1/de not_active IP Right Cessation
- 1999-05-04 JP JP2000547465A patent/JP4618886B2/ja not_active Expired - Fee Related
- 1999-05-04 CA CA2641613A patent/CA2641613C/fr not_active Expired - Fee Related
- 1999-05-04 WO PCT/IL1999/000232 patent/WO1999057550A1/fr not_active Ceased
- 1999-05-04 CA CA2820735A patent/CA2820735A1/fr not_active Abandoned
- 1999-05-04 AU AU36259/99A patent/AU3625999A/en not_active Abandoned
- 1999-05-04 CA CA002331688A patent/CA2331688C/fr not_active Expired - Fee Related
- 1999-05-04 EP EP99918252A patent/EP1075656B1/fr not_active Expired - Lifetime
2011
- 2011-08-03 US US13/197,294 patent/US20120309106A1/en not_active Abandoned
2016
- 2016-08-08 US US15/231,264 patent/US20170121761A1/en not_active Abandoned

Also Published As

Publication number	Publication date
US20120309106A1 (en)	2012-12-06
CA2331688C (fr)	2008-12-09
US20170121761A1 (en)	2017-05-04
ATE515693T1 (de)	2011-07-15
EP1075656A1 (fr)	2001-02-14
CA2641613A1 (fr)	1999-11-11
CA2331688A1 (fr)	1999-11-11
WO1999057550A1 (fr)	1999-11-11
AU3625999A (en)	1999-11-23
JP4618886B2 (ja)	2011-01-26
JP2002522748A (ja)	2002-07-23
CA2641613C (fr)	2013-06-25
CA2820735A1 (fr)	1999-11-11
IL124322A0 (en)	1998-12-06
EP1075656B1 (fr)	2011-07-06

Legal Events

Date	Code	Title
2002-11-10	FF	Patent granted
2002-12-01	KB	Patent renewed
2004-06-20	KB	Patent renewed
2008-11-03	KB	Patent renewed
2012-05-31	KB	Patent renewed
2016-11-30	MM9K	Patent not in force due to non-payment of renewal fees

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US7851149B2 (en)	2010-12-14	Method for gold deposition
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