IL125225A - Fsh and lh separation and purification process - Google Patents

Fsh and lh separation and purification process

Info

Publication number: IL125225A
Authority: IL; Israel
Prior art keywords: fsh; process according; chromatography column; water solution; buffer
Prior art date: 1996-11-07

Application number

IL12522597A

Other languages

English (en)

Other versions

IL125225A0 (en

Original Assignee

Ibsa Inst Biochimique Sa

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-11-07

Filing date

1997-11-03

Publication date

2002-04-21

1997-11-03 Application filed by Ibsa Inst Biochimique Sa filed Critical Ibsa Inst Biochimique Sa

1999-03-12 Publication of IL125225A0 publication Critical patent/IL125225A0/xx

2002-04-21 Publication of IL125225A publication Critical patent/IL125225A/en

Links

238000000746 purification Methods 0.000 title claims abstract description 23
238000000926 separation method Methods 0.000 title claims abstract description 9
238000000034 method Methods 0.000 claims abstract description 46
102000004169 proteins and genes Human genes 0.000 claims abstract description 36
108090000623 proteins and genes Proteins 0.000 claims abstract description 36
239000011347 resin Substances 0.000 claims abstract description 27
229920005989 resin Polymers 0.000 claims abstract description 27
238000011068 loading method Methods 0.000 claims abstract description 16
IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims abstract description 15
238000004255 ion exchange chromatography Methods 0.000 claims abstract description 13
125000000129 anionic group Chemical group 0.000 claims abstract description 11
238000001042 affinity chromatography Methods 0.000 claims abstract description 10
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 8
239000008363 phosphate buffer Substances 0.000 claims abstract description 8
230000003612 virological effect Effects 0.000 claims abstract description 7
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 claims abstract description 4
239000003446 ligand Substances 0.000 claims abstract description 4
239000011780 sodium chloride Substances 0.000 claims abstract description 4
239000000243 solution Substances 0.000 claims description 49
239000000872 buffer Substances 0.000 claims description 17
229920000936 Agarose Polymers 0.000 claims description 11
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 11
239000007986 glycine-NaOH buffer Substances 0.000 claims description 10
239000001488 sodium phosphate Substances 0.000 claims description 8
229910000162 sodium phosphate Inorganic materials 0.000 claims description 8
230000001143 conditioned effect Effects 0.000 claims description 6
210000002700 urine Anatomy 0.000 claims description 6
125000001453 quaternary ammonium group Chemical group 0.000 claims description 4
239000011159 matrix material Substances 0.000 claims description 3
239000008351 acetate buffer Substances 0.000 claims description 2
YKCWQPZFAFZLBI-UHFFFAOYSA-N cibacron blue Chemical group C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC=C1S(O)(=O)=O YKCWQPZFAFZLBI-UHFFFAOYSA-N 0.000 claims description 2
GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 claims 1
RZVHIXYEVGDQDX-UHFFFAOYSA-N 9,10-anthraquinone Chemical compound C1=CC=C2C(=O)C3=CC=CC=C3C(=O)C2=C1 RZVHIXYEVGDQDX-UHFFFAOYSA-N 0.000 abstract 1
108010079345 Follicle Stimulating Hormone Proteins 0.000 description 79
102000012673 Follicle Stimulating Hormone Human genes 0.000 description 79
229940028334 follicle stimulating hormone Drugs 0.000 description 79
108010073521 Luteinizing Hormone Proteins 0.000 description 63
102000009151 Luteinizing Hormone Human genes 0.000 description 63
229940040129 luteinizing hormone Drugs 0.000 description 61
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 26
230000000694 effects Effects 0.000 description 21
229940088597 hormone Drugs 0.000 description 17
239000005556 hormone Substances 0.000 description 17
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
108010057021 Menotropins Proteins 0.000 description 13
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 12
238000010828 elution Methods 0.000 description 11
238000004587 chromatography analysis Methods 0.000 description 9
239000000047 product Substances 0.000 description 8
239000006228 supernatant Substances 0.000 description 8
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 7
230000003287 optical effect Effects 0.000 description 7
239000002244 precipitate Substances 0.000 description 7
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 6
239000007974 sodium acetate buffer Substances 0.000 description 6
238000005119 centrifugation Methods 0.000 description 5
230000000717 retained effect Effects 0.000 description 5
238000003756 stirring Methods 0.000 description 5
230000002485 urinary effect Effects 0.000 description 5
238000011109 contamination Methods 0.000 description 4
239000000203 mixture Substances 0.000 description 4
150000003839 salts Chemical class 0.000 description 4
USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 3
239000005695 Ammonium acetate Substances 0.000 description 3
206010062767 Hypophysitis Diseases 0.000 description 3
229940043376 ammonium acetate Drugs 0.000 description 3
235000019257 ammonium acetate Nutrition 0.000 description 3
239000007853 buffer solution Substances 0.000 description 3
VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 3
239000001639 calcium acetate Substances 0.000 description 3
235000011092 calcium acetate Nutrition 0.000 description 3
229960005147 calcium acetate Drugs 0.000 description 3
210000004027 cell Anatomy 0.000 description 3
239000000284 extract Substances 0.000 description 3
239000012528 membrane Substances 0.000 description 3
210000003635 pituitary gland Anatomy 0.000 description 3
235000017550 sodium carbonate Nutrition 0.000 description 3
229910000029 sodium carbonate Inorganic materials 0.000 description 3
230000001225 therapeutic effect Effects 0.000 description 3
229940001496 tribasic sodium phosphate Drugs 0.000 description 3
238000000108 ultra-filtration Methods 0.000 description 3
238000005406 washing Methods 0.000 description 3
238000000035 BCA protein assay Methods 0.000 description 2
238000010521 absorption reaction Methods 0.000 description 2
239000007864 aqueous solution Substances 0.000 description 2
239000012888 bovine serum Substances 0.000 description 2
239000001913 cellulose Substances 0.000 description 2
229920002678 cellulose Polymers 0.000 description 2
238000011082 depyrogenation Methods 0.000 description 2
230000008030 elimination Effects 0.000 description 2
238000003379 elimination reaction Methods 0.000 description 2
239000004615 ingredient Substances 0.000 description 2
238000005342 ion exchange Methods 0.000 description 2
238000003760 magnetic stirring Methods 0.000 description 2
210000002381 plasma Anatomy 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
238000011084 recovery Methods 0.000 description 2
CIJQGPVMMRXSQW-UHFFFAOYSA-M sodium;2-aminoacetic acid;hydroxide Chemical compound O.[Na+].NCC([O-])=O CIJQGPVMMRXSQW-UHFFFAOYSA-M 0.000 description 2
238000011282 treatment Methods 0.000 description 2
JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 description 1
108020004414 DNA Proteins 0.000 description 1
229920001425 Diethylaminoethyl cellulose Polymers 0.000 description 1
206010020751 Hypersensitivity Diseases 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
241000700605 Viruses Species 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
239000001166 ammonium sulphate Substances 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
230000031018 biological processes and functions Effects 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
230000003750 conditioning effect Effects 0.000 description 1
239000000356 contaminant Substances 0.000 description 1
239000000287 crude extract Substances 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
230000035558 fertility Effects 0.000 description 1
238000001914 filtration Methods 0.000 description 1
238000004108 freeze drying Methods 0.000 description 1
238000007710 freezing Methods 0.000 description 1
230000008014 freezing Effects 0.000 description 1
230000006543 gametophyte development Effects 0.000 description 1
230000001456 gonadotroph Effects 0.000 description 1
230000003054 hormonal effect Effects 0.000 description 1
238000010255 intramuscular injection Methods 0.000 description 1
239000007927 intramuscular injection Substances 0.000 description 1
230000007774 longterm Effects 0.000 description 1
239000008176 lyophilized powder Substances 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000003960 organic solvent Substances 0.000 description 1
230000001766 physiological effect Effects 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
239000010453 quartz Substances 0.000 description 1
VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000001632 sodium acetate Substances 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
239000012064 sodium phosphate buffer Substances 0.000 description 1
239000007787 solid Substances 0.000 description 1
150000003431 steroids Chemical class 0.000 description 1
238000003860 storage Methods 0.000 description 1
239000000725 suspension Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Endocrinology (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Medicinal Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Zoology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Toxicology (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Reproductive Health (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Compounds Of Unknown Constitution (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Amplifiers (AREA)
Control Of Motors That Do Not Use Commutators (AREA)
External Artificial Organs (AREA)
Executing Machine-Instructions (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Treatment Of Liquids With Adsorbents In General (AREA)

IL12522597A 1996-11-07 1997-11-03 Fsh and lh separation and purification process IL125225A (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
IT96MI002313A IT1287138B1 (it)	1996-11-07	1996-11-07	Procedimento per la separazione e purificazione di fsh e lh
PCT/EP1997/006058 WO1998020039A1 (en)	1996-11-07	1997-11-03	Fsh and lh separation and purification process

Publications (2)

Publication Number	Publication Date
IL125225A0 IL125225A0 (en)	1999-03-12
IL125225A true IL125225A (en)	2002-04-21

Family

ID=11375168

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL12522597A IL125225A (en)	1996-11-07	1997-11-03	Fsh and lh separation and purification process

Country Status (10)

Country	Link
EP (1)	EP0882064B1 (de)
AT (1)	ATE236931T1 (de)
AU (1)	AU719560B2 (de)
CA (1)	CA2242325C (de)
DE (1)	DE69720693T2 (de)
ES (1)	ES2196380T3 (de)
IL (1)	IL125225A (de)
IT (1)	IT1287138B1 (de)
PT (1)	PT882064E (de)
WO (1)	WO1998020039A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6414123B1 (en)	1997-10-21	2002-07-02	Vitro Diagnostics, Inc.	Method for purifying FSH
BRPI0010677B8 (pt) *	1999-04-16	2021-05-25	Inst Massone S A	processo de purificação de gonadotropinas urinárias humanas a partir de gonadotropinas brutas.
US7022822B1 (en) *	1999-04-16	2006-04-04	Instituto Massone S.A.	Highly purified gonadotropin compositions and process for preparing them
EA006605B1 (ru) *	2000-02-22	2006-02-24	Апплайд Резеч Системз Арс Холдинг Н.В.	СПОСОБ ОЧИСТКИ РЕКОМБИНАНТНОГО чХГ
CZ303274B6 (cs) *	2000-02-22	2012-07-11	Merck Serono Sa	Zpusob vycištení luteinizacního hormonu
DE602005009856D1 (de)	2004-11-09	2008-10-30	Ares Trading Sa	Verfahren zur reinigung von fsh
WO2007022799A1 (en)	2005-08-26	2007-03-01	Ares Trading S.A.	Process for the preparation of glycosylated interferon beta
EP1960419B1 (de)	2005-12-09	2016-03-16	Ares Trading S.A.	Verfahren zur aufreinigung von fsh oder einer fsh-mutante
CN101397339B (zh) *	2008-09-24	2012-07-25	上海天伟生物制药有限公司	糖蛋白中去除/灭活病毒的方法
EP2325194A1 (de)	2009-11-24	2011-05-25	Glycotope GmbH	Verfahren zur Reinigung von Glykoproteinen
CN101792482B (zh) *	2010-03-12	2012-11-21	丽珠医药集团股份有限公司	一种促性腺激素的纯化方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS58157720A (ja) *	1982-03-15	1983-09-19	Nippon Chem Res Kk	高純度の胎盤性性腺刺戟ホルモンの製造法

1996
- 1996-11-07 IT IT96MI002313A patent/IT1287138B1/it active IP Right Grant
1997
- 1997-11-03 AT AT97950077T patent/ATE236931T1/de active
- 1997-11-03 CA CA002242325A patent/CA2242325C/en not_active Expired - Fee Related
- 1997-11-03 WO PCT/EP1997/006058 patent/WO1998020039A1/en not_active Ceased
- 1997-11-03 ES ES97950077T patent/ES2196380T3/es not_active Expired - Lifetime
- 1997-11-03 DE DE69720693T patent/DE69720693T2/de not_active Expired - Lifetime
- 1997-11-03 IL IL12522597A patent/IL125225A/en not_active IP Right Cessation
- 1997-11-03 PT PT97950077T patent/PT882064E/pt unknown
- 1997-11-03 AU AU53162/98A patent/AU719560B2/en not_active Ceased
- 1997-11-03 EP EP97950077A patent/EP0882064B1/de not_active Expired - Lifetime

Also Published As

Publication number	Publication date
WO1998020039A1 (en)	1998-05-14
AU5316298A (en)	1998-05-29
ITMI962313A1 (it)	1998-05-07
IL125225A0 (en)	1999-03-12
CA2242325A1 (en)	1998-05-14
ATE236931T1 (de)	2003-04-15
PT882064E (pt)	2003-08-29
AU719560B2 (en)	2000-05-11
EP0882064B1 (de)	2003-04-09
DE69720693D1 (de)	2003-05-15
DE69720693T2 (de)	2004-03-04
ITMI962313A0 (it)	1996-11-07
IT1287138B1 (it)	1998-08-04
ES2196380T3 (es)	2003-12-16
EP0882064A1 (de)	1998-12-09
CA2242325C (en)	2008-04-29

Legal Events

Date	Code	Title
2002-09-12	FF	Patent granted
2002-11-10	KB	Patent renewed
2004-01-04	KB	Patent renewed
2008-01-06	KB	Patent renewed
2011-11-30	KB	Patent renewed
2016-05-31	MM9K	Patent not in force due to non-payment of renewal fees

Publication	Publication Date	Title
Rosner et al.	1975	Isolation and characterization of the testosterone-estradiol-binding globulin from human plasma. Use of a novel affinity column
Boyd	1974	A protein-bound form of porcine renal renin
Cuatrecasas	1972	Isolation of the insulin receptor of liver and fat-cell membranes
Cuatrecasas	1972	Affinity chromatography and purification of the insulin receptor of liver cell membranes
Hathaway et al.	1983	Selective purification of the 20,000-Da light chains of smooth muscle myosin
Ward et al.	1959	Chromatography of luteinizing hormone from sheep pituitary glands
EP0882064B1 (de)	2003-04-09	Fsh und lh trennungs- und reinigungsverfahren
Quadros et al.	1986	Purification and molecular characterization of human transcobalamin II.
US6162905A (en)	2000-12-19	FSH and LH separation and purification process
Eylar et al.	1969	Allergic encephalomyelitis: Cleavage of the C-tryptophyl bond in the encephalitogenic basic protein from bovine myelin
O'Riordan et al.	1971	Isolation and chemical properties of porcine parathyroid hormone
Pottinger	1986	Estrogen-binding sites in the liver of sexually mature male and female brown trout, Salmo trutta L
IE47092B1 (en)	1983-12-14	New glycoprotein and process for isolating it
Reichert Jr	1975	[30] Purification of anterior pituitary hormones (ovine, bovine, rat, rabbit)
Free et al.	1966	Separation and properties of multiple components of bovine growth hormone
Cuatrecasas et al.	1974	[85] Insulin receptors
US4065445A (en)	1977-12-27	Pregnancy-specific β1 -glycoprotein and process for isolating it
JP2001323000A (ja)	2001-11-20	Ｆｓｈ（卵胞刺激ホルモン）とｌｈ（黄体化ホルモン）の分離と精製工程
Movat et al.	1969	A small molecular weight permeability factor in guinea pig serum: adsorption to antigen-antibody aggregates
Esch et al.	1983	[5] Microisolation of neuropeptides
US3310471A (en)	1967-03-21	Placental lactogenic factor process
Hargrave et al.	1973	A preparative method for the isolation of carboxyl terminal tryptic peptides from proteins
US4599318A (en)	1986-07-08	Tissue protein PP19, a process for obtaining it, and its use
Sairam et al.	1981	Binding of an inhibin-like protein from bull seminal plasma to ovine pituitary membranes
Abou-Issa et al.	1980	Properties of solubilized and purified follitropin receptor from calf testis