IL182143A - Cultivation of cultured primate embryonic stem cells expressing oct4, ssea4 or tra1-60 - Google Patents

Cultivation of cultured primate embryonic stem cells expressing oct4, ssea4 or tra1-60

Info

Publication number: IL182143A
Authority: IL; Israel
Prior art keywords: stem cells; medium; cells; culture; growth factor
Prior art date: 2004-09-28

Application number

IL182143A

Other languages

English (en)

Other versions

IL182143A0 (en

Original Assignee

Wisconsin Alumni Res Found

Wicell Res Inst Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2004-09-28

Filing date

2007-03-22

Publication date

2011-11-30

2004-09-28 Priority claimed from US10/952,096 external-priority patent/US20050148070A1/en

2005-03-11 Priority claimed from US11/078,737 external-priority patent/US7439064B2/en

2007-03-22 Application filed by Wisconsin Alumni Res Found, Wicell Res Inst Inc filed Critical Wisconsin Alumni Res Found

2007-07-24 Publication of IL182143A0 publication Critical patent/IL182143A0/en

2011-11-30 Publication of IL182143A publication Critical patent/IL182143A/en

Links

210000001671 embryonic stem cell Anatomy 0.000 title claims description 26
241000288906 Primates Species 0.000 title claims description 21
210000004027 cell Anatomy 0.000 claims description 121
239000002609 medium Substances 0.000 claims description 44
210000002966 serum Anatomy 0.000 claims description 43
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 42
210000000130 stem cell Anatomy 0.000 claims description 33
102000018233 Fibroblast Growth Factor Human genes 0.000 claims description 24
108050007372 Fibroblast Growth Factor Proteins 0.000 claims description 24
108090000379 Fibroblast growth factor 2 Proteins 0.000 claims description 23
229940126864 fibroblast growth factor Drugs 0.000 claims description 23
102000004877 Insulin Human genes 0.000 claims description 22
108090001061 Insulin Proteins 0.000 claims description 22
238000000034 method Methods 0.000 claims description 22
229940125396 insulin Drugs 0.000 claims description 21
102000004338 Transferrin Human genes 0.000 claims description 20
108090000901 Transferrin Proteins 0.000 claims description 20
239000001963 growth medium Substances 0.000 claims description 20
239000012581 transferrin Substances 0.000 claims description 20
238000012258 culturing Methods 0.000 claims description 16
230000001605 fetal effect Effects 0.000 claims description 15
238000012423 maintenance Methods 0.000 claims description 14
241001465754 Metazoa Species 0.000 claims description 11
108010088751 Albumins Proteins 0.000 claims description 10
102000009027 Albumins Human genes 0.000 claims description 10
150000001413 amino acids Chemical class 0.000 claims description 10
230000035755 proliferation Effects 0.000 claims description 9
102100037665 Fibroblast growth factor 9 Human genes 0.000 claims description 8
101001027380 Homo sapiens Fibroblast growth factor 9 Proteins 0.000 claims description 8
102100035323 Fibroblast growth factor 18 Human genes 0.000 claims description 7
101000878128 Homo sapiens Fibroblast growth factor 18 Proteins 0.000 claims description 7
239000006143 cell culture medium Substances 0.000 claims description 6
239000003636 conditioned culture medium Substances 0.000 claims description 6
230000002062 proliferating effect Effects 0.000 claims description 6
230000003213 activating effect Effects 0.000 claims description 5
239000003102 growth factor Substances 0.000 claims description 5
210000003981 ectoderm Anatomy 0.000 claims description 2
210000003716 mesoderm Anatomy 0.000 claims description 2
102100035290 Fibroblast growth factor 13 Human genes 0.000 claims 8
229910052500 inorganic mineral Inorganic materials 0.000 claims 8
239000011707 mineral Substances 0.000 claims 8
229940088594 vitamin Drugs 0.000 claims 8
229930003231 vitamin Natural products 0.000 claims 8
235000013343 vitamin Nutrition 0.000 claims 8
239000011782 vitamin Substances 0.000 claims 8
102100035308 Fibroblast growth factor 17 Human genes 0.000 claims 6
102100028072 Fibroblast growth factor 4 Human genes 0.000 claims 6
101000878124 Homo sapiens Fibroblast growth factor 17 Proteins 0.000 claims 6
101001060274 Homo sapiens Fibroblast growth factor 4 Proteins 0.000 claims 6
210000001778 pluripotent stem cell Anatomy 0.000 claims 5
102000035025 signaling receptors Human genes 0.000 claims 4
108091005475 signaling receptors Proteins 0.000 claims 4
150000003839 salts Chemical class 0.000 claims 2
210000001900 endoderm Anatomy 0.000 claims 1
101150021185 FGF gene Proteins 0.000 description 19
210000002950 fibroblast Anatomy 0.000 description 14
102100024785 Fibroblast growth factor 2 Human genes 0.000 description 12
238000004113 cell culture Methods 0.000 description 10
238000001943 fluorescence-activated cell sorting Methods 0.000 description 8
238000010367 cloning Methods 0.000 description 7
230000001143 conditioned effect Effects 0.000 description 5
230000002035 prolonged effect Effects 0.000 description 5
238000012360 testing method Methods 0.000 description 5
238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 4
241000699666 Mus <mouse, genus> Species 0.000 description 4
230000004069 differentiation Effects 0.000 description 4
238000002474 experimental method Methods 0.000 description 4
210000001519 tissue Anatomy 0.000 description 4
102000003974 Fibroblast growth factor 2 Human genes 0.000 description 3
150000001875 compounds Chemical class 0.000 description 3
CBVWMGCJNPPAAR-HJWRWDBZSA-N (nz)-n-(5-methylheptan-3-ylidene)hydroxylamine Chemical compound CCC(C)C\C(CC)=N/O CBVWMGCJNPPAAR-HJWRWDBZSA-N 0.000 description 2
241000283690 Bos taurus Species 0.000 description 2
101000812663 Homo sapiens Endoplasmin Proteins 0.000 description 2
101000689394 Homo sapiens Phospholipid scramblase 4 Proteins 0.000 description 2
101000796673 Homo sapiens Transformation/transcription domain-associated protein Proteins 0.000 description 2
101001067395 Mus musculus Phospholipid scramblase 1 Proteins 0.000 description 2
102100024494 Phospholipid scramblase 4 Human genes 0.000 description 2
206010043276 Teratoma Diseases 0.000 description 2
102000004142 Trypsin Human genes 0.000 description 2
108090000631 Trypsin Proteins 0.000 description 2
210000004748 cultured cell Anatomy 0.000 description 2
229940079593 drug Drugs 0.000 description 2
239000003814 drug Substances 0.000 description 2
210000001654 germ layer Anatomy 0.000 description 2
230000002401 inhibitory effect Effects 0.000 description 2
210000001161 mammalian embryo Anatomy 0.000 description 2
230000007246 mechanism Effects 0.000 description 2
239000000203 mixture Substances 0.000 description 2
235000015097 nutrients Nutrition 0.000 description 2
239000008188 pellet Substances 0.000 description 2
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
238000011160 research Methods 0.000 description 2
238000002054 transplantation Methods 0.000 description 2
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 2
239000012588 trypsin Substances 0.000 description 2
241000282693 Cercopithecidae Species 0.000 description 1
102000008186 Collagen Human genes 0.000 description 1
108010035532 Collagen Proteins 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
101001052035 Homo sapiens Fibroblast growth factor 2 Proteins 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
241000699670 Mus sp. Species 0.000 description 1
239000004677 Nylon Substances 0.000 description 1
229930040373 Paraformaldehyde Natural products 0.000 description 1
BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
229910000831 Steel Inorganic materials 0.000 description 1
206010043275 Teratogenicity Diseases 0.000 description 1
102000002070 Transferrins Human genes 0.000 description 1
108010015865 Transferrins Proteins 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
230000002411 adverse Effects 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
210000004102 animal cell Anatomy 0.000 description 1
210000004271 bone marrow stromal cell Anatomy 0.000 description 1
239000012888 bovine serum Substances 0.000 description 1
230000000747 cardiac effect Effects 0.000 description 1
210000000845 cartilage Anatomy 0.000 description 1
238000010370 cell cloning Methods 0.000 description 1
230000004663 cell proliferation Effects 0.000 description 1
GPUADMRJQVPIAS-QCVDVZFFSA-M cerivastatin sodium Chemical compound [Na+].COCC1=C(C(C)C)N=C(C(C)C)C(\C=C\[C@@H](O)C[C@@H](O)CC([O-])=O)=C1C1=CC=C(F)C=C1 GPUADMRJQVPIAS-QCVDVZFFSA-M 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
208000037516 chromosome inversion disease Diseases 0.000 description 1
230000001886 ciliary effect Effects 0.000 description 1
229920001436 collagen Polymers 0.000 description 1
238000011109 contamination Methods 0.000 description 1
238000009795 derivation Methods 0.000 description 1
238000011161 development Methods 0.000 description 1
206010012601 diabetes mellitus Diseases 0.000 description 1
108010007093 dispase Proteins 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
210000000981 epithelium Anatomy 0.000 description 1
239000000945 filler Substances 0.000 description 1
238000009472 formulation Methods 0.000 description 1
210000003958 hematopoietic stem cell Anatomy 0.000 description 1
239000004026 insulin derivative Substances 0.000 description 1
208000032839 leukemia Diseases 0.000 description 1
239000003446 ligand Substances 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
230000007774 longterm Effects 0.000 description 1
230000001537 neural effect Effects 0.000 description 1
230000000508 neurotrophic effect Effects 0.000 description 1
229920001778 nylon Polymers 0.000 description 1
229920002866 paraformaldehyde Polymers 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
239000000047 product Substances 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
229910052711 selenium Inorganic materials 0.000 description 1
239000011669 selenium Substances 0.000 description 1
230000011664 signaling Effects 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
241000894007 species Species 0.000 description 1
230000003068 static effect Effects 0.000 description 1
239000010959 steel Substances 0.000 description 1
239000013589 supplement Substances 0.000 description 1
230000003319 supportive effect Effects 0.000 description 1
230000002195 synergetic effect Effects 0.000 description 1
231100000211 teratogenicity Toxicity 0.000 description 1
231100000419 toxicity Toxicity 0.000 description 1
230000001988 toxicity Effects 0.000 description 1
235000019195 vitamin supplement Nutrition 0.000 description 1

Landscapes

Micro-Organisms Or Cultivation Processes Thereof (AREA)

IL182143A 2004-09-28 2007-03-22 Cultivation of cultured primate embryonic stem cells expressing oct4, ssea4 or tra1-60 IL182143A (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US10/952,096 US20050148070A1 (en)	2000-03-09	2004-09-28	Cultivation of primate embryonic stem cells
US11/078,737 US7439064B2 (en)	2000-03-09	2005-03-11	Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
PCT/US2005/034510 WO2006036925A1 (en)	2004-09-28	2005-09-27	Cultivation of primate embryonic stem cells

Publications (2)

Publication Number	Publication Date
IL182143A0 IL182143A0 (en)	2007-07-24
IL182143A true IL182143A (en)	2011-11-30

Family

ID=42333917

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL182143A IL182143A (en)	2004-09-28	2007-03-22	Cultivation of cultured primate embryonic stem cells expressing oct4, ssea4 or tra1-60

Country Status (3)

Country	Link
JP (3)	JP6216997B2 (es)
ES (1)	ES2800973T3 (es)
IL (1)	IL182143A (es)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA2324591A1 (en) *	1998-04-09	1999-10-21	Bresagen Limited	Cell differentiation/proliferation and maintenance factor and uses thereof
US7410798B2 (en) *	2001-01-10	2008-08-12	Geron Corporation	Culture system for rapid expansion of human embryonic stem cells
US7005252B1 (en) *	2000-03-09	2006-02-28	Wisconsin Alumni Research Foundation	Serum free cultivation of primate embryonic stem cells

2005
- 2005-09-27 ES ES05801117T patent/ES2800973T3/es active Active
2007
- 2007-03-22 IL IL182143A patent/IL182143A/en active IP Right Grant
2012
- 2012-03-21 JP JP2012064507A patent/JP6216997B2/ja not_active Expired - Lifetime
2016
- 2016-09-23 JP JP2016186252A patent/JP6314193B2/ja not_active Expired - Lifetime
2017
- 2017-04-17 JP JP2017081516A patent/JP6446496B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
JP2012110354A (ja)	2012-06-14
JP6446496B2 (ja)	2018-12-26
IL182143A0 (en)	2007-07-24
JP6314193B2 (ja)	2018-04-18
JP2016208999A (ja)	2016-12-15
JP2017121262A (ja)	2017-07-13
JP6216997B2 (ja)	2017-10-25
ES2800973T3 (es)	2021-01-07

Legal Events

Date	Code	Title
2012-03-29	FF	Patent granted
2012-06-28	KB	Patent renewed
2015-08-31	KB	Patent renewed
2019-09-26	KB	Patent renewed

Publication	Publication Date	Title
CA2582566C (en)	2014-09-09	Cultivation of primate embryonic stem cells
CN100372928C (zh)	2008-03-05	灵长类动物胚胎干细胞的无血清培养物
JP5227318B2 (ja)	2013-07-03	細胞増殖培地
AU2001241973A1 (en)	2001-11-29	Serum free cultivation of primate embryonic stem cells
US20120178160A1 (en)	2012-07-12	Cultivation Of Primate Embryonic Stem Cells
IL182143A (en)	2011-11-30	Cultivation of cultured primate embryonic stem cells expressing oct4, ssea4 or tra1-60
AU2007200575B2 (en)	2010-01-21	Serum free cultivation of primate embryonic stem cells
HK1053616B (en)	2008-08-22	Serum free cultivation of primate embryonic stem cells