IL257261A - Method of preparing plant-derived proteins - Google Patents

Method of preparing plant-derived proteins

Info

Publication number
IL257261A
IL257261A IL257261A IL25726118A IL257261A IL 257261 A IL257261 A IL 257261A IL 257261 A IL257261 A IL 257261A IL 25726118 A IL25726118 A IL 25726118A IL 257261 A IL257261 A IL 257261A
Authority
IL
Israel
Prior art keywords
plant
protein
proteins
chimeric
complex
Prior art date
Application number
IL257261A
Other languages
Hebrew (he)
Other versions
IL257261B (en
Original Assignee
Medicago Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medicago Inc filed Critical Medicago Inc
Publication of IL257261A publication Critical patent/IL257261A/en
Publication of IL257261B publication Critical patent/IL257261B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/517—Plant cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Pulmonology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Water Supply & Treatment (AREA)
  • Analytical Chemistry (AREA)

Claims (31)

:
1. A method of preparing plant-derived proteins, or protein suprastructure, comprising: a. obtaining a plant or plant matter comprising apoplast-localized proteins, or suprastructure proteins, wherein the plant matter consist of plant leaves, leaf pieces, shredded leaves, or a combination thereof; b. producing a protoplast/spheroplast fraction and an apoplast fraction by treating the plant or plant matter with a cell wall degrading enzyme mixture comprising one or more than one cellulase and a pectinase, 20 mM to 750 mM of a salt, and a buffer or buffer system which maintains the pH in the range of 5 to 6.6, at room temperature; and, c. recovering the apoplast fraction, the apoplast fraction comprising the plant-derived proteins, or suprastructure proteins, wherein the protein suprastructures have a molecular weight from 75 to 1500 kDa.
2. The method of claim 1, wherein the cell wall degrading enzyme mixture further comprises an osmoticum.
3. The method of claim 1, wherein the cell wall degrading enzyme mixture does not include one or more of a lipase, or a protease.
4. The method of claim 1 wherein in the step of obtaining (step a), the plant is transformed with a nucleic acid comprising a nucleotide sequence encoding the proteins, or suprastructure proteins, is selected from the group of a peptide, a protein, a protein rosette, a protein complex, a proteasome, a metabolon, a transcription complex, a recombination complex, a photosynthetic complex, a membrane transport complex, a nuclear pore complex, a protein nanoparticle, a glycoprotein, an antibody, a polyclonal antibody, a monoclonal antibody, a single chain monoclonal antibody, a viral envelope protein, a viral structural protein, a viral capsid protein, and a viral coat protein, a chimeric protein, a chimeric protein complex, a chimeric protein nanoparticle, a chimeric glycoprotein, a chimeric antibody, a chimeric monoclonal antibody, a chimeric single chain monoclonal antibody, a chimeric hemagglutinin, and the plant or plant matter is harvested. 61 257261/3
5. The method of claim 4, wherein the nucleic acid is introduced into the plant in a transient manner.
6. The method of claim 4, wherein, the nucleic acid is stably integrated within a genome of the plant.
7. The method of claim 1 wherein in the step of obtaining (step a), the plant is grown and the plant or plant matter is harvested.
8. The method of claim 4, wherein the nucleic acid encodes a monoclonal antibody or an influenza hemagglutinin.
9. The method of claim 1 further comprising a step of d) purifying the plant-derived proteins, or suprastructure proteins, from the apoplast fraction.
10. The method of claim 9, wherein the step of purifying comprises filtering the apoplast fraction using depth filtration to produce a clarified extract, followed by chromatography of the clarified extract using size exclusion chromatography, cation exchange resin or affinity chromatography, or a combination thereof.
11. A method of preparing plant-derived proteins, or protein suprastructure, comprising: a. obtaining a plant or plant matter comprising plant-derived proteins or suprastructure proteins, wherein the plant matter consist of plant leaves, leaf pieces, shredded leaves, or a combination thereof; b. digesting the plant or plant matter using a cell wall degrading enzyme mixture consisting of cellulase, or a cell wall degrading enzyme mixture comprising one or more than one cellulase and a pectinase, 20 mM to 750 mM of a salt, and a buffer or buffer system which maintains the pH in the range of 5 to 6.6, at room temperature to produce a digested fraction, c. filtering the digested fraction to produce a filtered fraction and recovering the plant- derived proteins, or suprastructure proteins, from the filtered fraction, wherein the protein suprastructures have a molecular weight from 75 to 1500 kDa. 62 257261/3
12. The method of claim 11 wherein in the step of obtaining (step a), the plant is grown and the plant or plant matter is harvested.
13. The method of claim 11, wherein the cell wall degrading enzyme mixture further comprises an osmoticum.
14. The method of claim 11, wherein the cell wall degrading enzyme mixture does not include one or more of a lipase, or a protease.
15. The method of claim 11, wherein in the step of obtaining (step a), the plant is transformed with a nucleic acid comprising a nucleotide sequence encoding the proteins, or suprastructure proteins, is selected from the group of a peptide, a protein, a protein rosette, a protein complex, a proteasome, a metabolon, a transcription complex, a recombination complex, a photosynthetic complex, a membrane transport complex, a nuclear pore complex, a protein nanoparticle, a glycoprotein, an antibody, a polyclonal antibody, a monoclonal antibody, a single chain monoclonal antibody, a viral envelope protein, a viral structural protein, a viral capsid protein, and a viral coat protein, a chimeric protein, a chimeric protein complex, a chimeric protein nanoparticle, a chimeric glycoprotein, a chimeric antibody, a chimeric monoclonal antibody, a chimeric single chain monoclonal antibody, a chimeric hemagglutinin, and the plant or plant matter is harvested.
16. The method of claim 15, wherein the nucleic acid is introduced into the plant in a transient manner.
17. The method of claim 15, wherein, the nucleic acid is stably integrated within a genome of the plant.
18. The method of claim 15, wherein the plant-derived proteins, or suprastructure proteins, comprise a monoclonal antibody or an influenza hemagglutinin.
19. The method of claim 11, further comprising a step d) of separating the proteins or protein suprastructure, in the filtered fraction from the cellular debris and insoluble materials.
20. The method of claim 19, wherein the step of separating is performed by centrifugation.
21. The method of claim 19, wherein the step of separating is performed by depth filtration. 63 257261/3
22. The method of claim 11, further comprising the step of d) purifying the plant-derived proteins or protein suprastructure, from the filtered fraction.
23. The method of claim 22, wherein the step of purifying comprises depth filtration of the filtered fraction to produce a clarified extract, followed by chromatography of the clarified extract using a cation exchange resin, a size exclusion resin, an affinity resin, or a combination thereof. 2+ 2+ + +
24. The method of claim 1, wherein the salt comprises Ca , Mg , Na , K , NaCl, CaCl , 2 CuSO or KNO . 4 3 2+ 2+ + +
25. The method of claim 11, wherein the salt comprises Ca , Mg , Na , K , NaCl, CaCl , 2 CuSO or KNO . 4 3
26. The method of claim 1, wherein the cell wall degrading enzyme mixture further comprises 5 mM to 200 mM EDTA or EGTA.
27. The method of claim 1, wherein the cell wall degrading enzyme mixture further comprises 125 mM citrate or less.
28. The method of claim 11, wherein the cell wall degrading enzyme mixture further comprises 5 mM to 200 mM EDTA or EGTA.
29. The method of claim 11, wherein the cell wall degrading enzyme mixture further comprises 125 mM citrate or less.
30. The method of claim 1, wherein the step of producing (step b) is conducted at a o o temperature of 18 C to 24 C. 64 257261/3
31. The method of claim 11, wherein the step of digesting (step b) is conducted at a o o temperature of 18 C to 24 C. For the Applicants, REINHOLD COHN AND PARTNERS By: 65
IL257261A 2009-09-22 2018-01-31 Method of preparing plant-derived proteins IL257261B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24478609P 2009-09-22 2009-09-22
PCT/CA2010/001489 WO2011035423A1 (en) 2009-09-22 2010-09-21 Method of preparing plant-derived proteins

Publications (2)

Publication Number Publication Date
IL257261A true IL257261A (en) 2018-03-29
IL257261B IL257261B (en) 2020-03-31

Family

ID=43795254

Family Applications (4)

Application Number Title Priority Date Filing Date
IL218393A IL218393A0 (en) 2009-09-22 2012-02-29 Method of preparign plant-derived vlps
IL218422A IL218422B (en) 2009-09-22 2012-03-01 Method of preparing plant-derived proteins
IL257261A IL257261B (en) 2009-09-22 2018-01-31 Method of preparing plant-derived proteins
IL270661A IL270661B (en) 2009-09-22 2019-11-14 A method for preparing vlps that are plant histories

Family Applications Before (2)

Application Number Title Priority Date Filing Date
IL218393A IL218393A0 (en) 2009-09-22 2012-02-29 Method of preparign plant-derived vlps
IL218422A IL218422B (en) 2009-09-22 2012-03-01 Method of preparing plant-derived proteins

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL270661A IL270661B (en) 2009-09-22 2019-11-14 A method for preparing vlps that are plant histories

Country Status (28)

Country Link
US (2) US11833200B2 (en)
EP (3) EP2480560B1 (en)
JP (2) JP5551780B2 (en)
KR (4) KR101773431B1 (en)
CN (4) CN107090021A (en)
AR (2) AR078432A1 (en)
AU (2) AU2010300033B2 (en)
BR (2) BR112012006414B1 (en)
CA (2) CA2772962C (en)
DK (3) DK2480658T3 (en)
ES (3) ES2911037T3 (en)
HR (3) HRP20220478T1 (en)
HU (3) HUE058165T2 (en)
IL (4) IL218393A0 (en)
IN (2) IN2012DN02637A (en)
MX (2) MX2012003372A (en)
MY (2) MY158475A (en)
NO (2) NO2480658T3 (en)
NZ (2) NZ598481A (en)
PH (2) PH12012500566A1 (en)
PL (3) PL2480658T3 (en)
PT (3) PT3354657T (en)
RU (2) RU2579903C2 (en)
SG (2) SG178918A1 (en)
SI (3) SI2480658T1 (en)
TR (1) TR201803560T4 (en)
WO (2) WO2011035422A1 (en)
ZA (2) ZA201202836B (en)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
WO2009076778A1 (en) 2007-11-27 2009-06-25 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
HUE039100T2 (en) 2009-06-24 2018-12-28 Medicago Inc Chimeric influenza virus-like particles comprising hemagglutinin
KR101773431B1 (en) * 2009-09-22 2017-09-12 메디카고 인코포레이티드 Method of preparing plant-derived vlps
TWI526539B (en) 2010-12-22 2016-03-21 苜蓿股份有限公司 Method for producing viroid-like particles (VLP) in plants and VLP produced by the method
TWI620816B (en) 2011-03-23 2018-04-11 苜蓿股份有限公司 Plant derived protein recovery method
KR101963792B1 (en) 2011-06-13 2019-07-31 메디카고 인코포레이티드 Rabies virus like particle production in plants
EP2758038B1 (en) * 2011-09-23 2018-05-30 The United States of America, as represented by The Secretary, Department of Health & Human Services Novel influenza hemagglutinin protein-based vaccines
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
NZ622731A (en) 2011-09-30 2016-04-29 Medicago Inc Increasing virus-like particle yield in plants
EP2798059B1 (en) * 2011-12-21 2021-09-29 Apse, Llc Process for purifying vlps with capsids resistant to hydrolases
JP6406655B2 (en) * 2012-02-22 2018-10-17 三菱商事フードテック株式会社 Method for softening vegetable food material, softened preparation, softened vegetable food material and food using the same
ES2657875T3 (en) * 2012-03-22 2018-03-07 Fraunhofer Usa Inc. Virus type particles comprising a matrix protein of an encapsulated plant virus and uses thereof
JP2015528285A (en) 2012-09-05 2015-09-28 メディカゴ インコーポレイテッド Production of picornavirus-like particles in plants
US9617297B2 (en) * 2012-10-11 2017-04-11 The Regents Of The University Of California Apoplast wash fluid recovery for improved recombinant endoglucanase extraction in tabacco leaves
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
ES2803508T3 (en) * 2013-03-28 2021-01-27 Medicago Inc Production of flu virus-like particles in plants
JP6358257B2 (en) * 2013-09-06 2018-07-18 三菱ケミカル株式会社 Protein production method using plants
WO2015054639A1 (en) 2013-10-11 2015-04-16 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Epstein-barr virus vaccines
RU2699982C2 (en) 2014-01-10 2019-09-11 Медикаго Инк. Enhancer elements cpmv
RU2731295C2 (en) 2014-07-11 2020-09-01 Медикаго Инк. Modification of protein production in plants
US11191727B2 (en) 2014-12-31 2021-12-07 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Multivalent nanoparticle-based vaccines
JP2019129705A (en) * 2016-03-31 2019-08-08 日本たばこ産業株式会社 Method for introducing substance into plant
SG10201912944QA (en) 2016-09-02 2020-02-27 The Usa As Represented By The Secretary Stabilized group 2 influenza hemagglutinin stem region trimers and uses thereof
WO2019104439A1 (en) * 2017-11-30 2019-06-06 Medicago Inc. Modified norovirus vp1 proteins and vlps comprising modified norovirus vp1 proteins
EP3814509A4 (en) 2018-06-27 2022-07-27 Medicago Inc. INFLUENZA VIRUS HEMAGGLUTININ MUTANTS
CA3106471A1 (en) * 2018-07-13 2020-01-16 Medicago Inc. Modified norovirus vp1 proteins and vlps comprising modified norovirus vp1 proteins
CN109112093B (en) * 2018-09-05 2021-11-09 上海交通大学 Method for efficient separation and instantaneous transformation of artemisia apiacea protoplast
CA3137447A1 (en) * 2019-04-25 2020-10-29 Flagship Pioneering Innovations Vi, Llc Compositions and methods relating to plant messenger packs
CN110483616B (en) * 2019-07-31 2021-06-04 中国农业科学院农产品加工研究所 Method for separating apoplast effector protein secreted by pathogenic bacteria from plant tissue infected by pathogenic bacteria
CN111060696B (en) * 2019-12-27 2023-09-08 湖南农业大学 Method for reducing false positive rate of plant small molecule signal peptide
CN111718394A (en) * 2020-06-28 2020-09-29 广西壮族自治区农业科学院 A method for extracting denatured protein from sugarcane tissue based on BPP method
CN111826394A (en) * 2020-08-07 2020-10-27 浙江华缔药业集团医药开发有限公司 Construction and application of plant transient expression vector
WO2022260849A1 (en) 2021-06-09 2022-12-15 Nant Holdings Ip, Llc Methods and systems for producing a protein of interest in a plant
CN114934007B (en) * 2022-07-01 2023-11-24 广东省农业科学院作物研究所 Preparation method of sweet potato protoplast
JP7745040B1 (en) 2024-06-21 2025-09-26 デンカ株式会社 Method for recovering and producing target protein
CN120082594B (en) * 2025-04-07 2025-10-28 贵州大学 Application of NbClpT2 in regulating plant resistance to PVY infection

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
EP0203177A4 (en) 1984-11-29 1987-04-28 Scripps Clinic Res Polypeptides and antibodies related to deglycosylated viral glycoproteins.
JPS61265086A (en) * 1985-05-21 1986-11-22 Mitsui Toatsu Chem Inc Method of cultivating protoplast
US5232833A (en) 1988-09-14 1993-08-03 Stressgen Biotechnologies Corporation Accumulation of heat shock proteins for evaluating biological damage due to chronic exposure of an organism to sublethal levels of pollutants
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
ATE142883T1 (en) 1991-03-28 1996-10-15 Rooperol Na Nv COMPOSITIONS OF PHYTOSTEROLS WITH PHYTOSTEROLINS AS IMMUNE MODULATORS
UA48104C2 (en) 1991-10-04 2002-08-15 Новартіс Аг Dna fragment including sequence that codes an insecticide protein with optimization for corn, dna fragment providing directed preferable for the stem core expression of the structural gene of the plant related to it, dna fragment providing specific for the pollen expression of related to it structural gene in the plant, recombinant dna molecule, method for obtaining a coding sequence of the insecticide protein optimized for corn, method of corn plants protection at least against one pest insect
US6326470B1 (en) 1997-04-15 2001-12-04 The Penn State Research Foundation Enhancement of accessibility of cellulose by expansins
US5762939A (en) 1993-09-13 1998-06-09 Mg-Pmc, Llc Method for producing influenza hemagglutinin multivalent vaccines using baculovirus
GB9414118D0 (en) 1994-07-13 1994-08-31 Axis Genetics Ltd Modified plant viruses as vectors of heterologous peptides
AU6504796A (en) 1995-07-20 1997-02-18 Washington State University Research Foundation Production of secreted foreign polypeptides in plant cell culture
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US6042832A (en) 1996-08-28 2000-03-28 Thomas Jefferson University Polypeptides fused with alfalfa mosaic virus or ilarvirus capsid proteins
US20010006950A1 (en) 1998-02-11 2001-07-05 Juha Punnonen Genetic vaccine vector engineering
US6489537B1 (en) 1998-08-07 2002-12-03 The Trustees Of The University Of Pennsylvania Phytochelatin synthases and uses therefor
IL141312A0 (en) 1998-08-11 2002-03-10 Biosource Tech Inc Method for recovering proteins from the interstitial fluid of plant tissues
US6287570B1 (en) 1998-11-23 2001-09-11 Patricia L. Foley Vaccine against swine influenza virus
JP2002533090A (en) 1998-12-23 2002-10-08 ザ、サミュアル、ラバツ、ノゥブル、ファウンデイシャン、インク Plant transformation method
FR2791358B1 (en) 1999-03-22 2003-05-16 Meristem Therapeutics CHEMICAL EXPRESSION PROMOTERS, EXPRESSION CASSETTES, PLASMIDS, VECTORS, PLANTS AND TRANSGENIC SEEDS CONTAINING THEM AND METHODS OF OBTAINING THEM
WO2000063400A2 (en) 1999-04-21 2000-10-26 The Samuel Roberts Noble Foundation Plant transformation process
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
WO2002012454A1 (en) * 2000-08-08 2002-02-14 The Kitasato Institute Virus-like micrograins and process for producing the same
CA2435000C (en) 2001-01-18 2014-03-25 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Oligomeric complexes of chimeric proteins with enhanced immunogenic potential
US20050037420A1 (en) 2001-09-14 2005-02-17 Mei-Yun Zhang Immunoglobulin having particular framework scaffold and methods of making and using
KR101113432B1 (en) 2002-02-13 2012-02-29 위스콘신 얼럼나이 리서어치 화운데이션 Signal for Packaging of Influenza Virus Vectors
US20040121465A1 (en) 2002-02-14 2004-06-24 Novavax, Inc. Optimization of gene sequences of virus-like particles for expression in insect cells
CN1665934B (en) 2002-03-19 2010-05-26 国际植物研究所 Expression of GNTIII (UDP-N-acetylglucosamine:β-D-mannoside β(1,4)-N-acetylglucosaminyl-transferase III) in plants
BRPI0410342A (en) 2003-05-05 2006-06-20 Dow Agrosciences Llc stable immunoprophylactic and therapeutic compositions derived from transgenic plant cells and processes for producing
KR20060035596A (en) * 2003-05-05 2006-04-26 보이스 톰슨 인스터튜트 포 플랜트 리서치 Vectors and Cells for the Preparation of Immunoprotective Compositions Derived from Foreign Gene Transducing Plants
US7566458B2 (en) 2003-06-16 2009-07-28 Medimmune, Llc Influenza hemagglutinin and neuraminidase variants
US8592197B2 (en) 2003-07-11 2013-11-26 Novavax, Inc. Functional influenza virus-like particles (VLPs)
CN1560227A (en) * 2004-02-25 2005-01-05 福建师范大学 Preparation and regeneration technology of protoplasts from Haematococcus pluvialis
WO2006016380A2 (en) 2004-08-13 2006-02-16 Council Of Scientific And Industrial Research A chimeric g protein based rabies vaccine
US9155483B2 (en) 2004-12-03 2015-10-13 The Invention Science Fund I, Llc Vision modification with reflected image
CA2611859C (en) * 2005-03-15 2015-03-31 Verenium Corporation Cellulases, nucleic acids encoding them and methods for making and using them
ES2662118T3 (en) 2005-04-29 2018-04-05 University Of Cape Town Expression of viral proteins in plants
CN100410378C (en) 2005-05-09 2008-08-13 中国农业科学院生物技术研究所 Gene encoding avian influenza hemagglutinin and its plant expression vector and application
CA2613160A1 (en) * 2005-06-24 2006-12-28 Bayer Bioscience N.V. Methods for altering the reactivity of plant cell walls
CA2615658A1 (en) 2005-07-19 2007-01-25 Dow Global Technolgies Inc. Recombinant flu vaccines
US7871626B2 (en) 2005-08-04 2011-01-18 St. Jude Children's Research Hospital Modified influenza virus for monitoring and improving vaccine efficiency
KR101359723B1 (en) 2005-10-18 2014-02-05 노바백스, 인코포레이티드 Functional influenza virus like particles(VLPS)
ES2514316T3 (en) * 2005-11-22 2014-10-28 Novartis Vaccines And Diagnostics, Inc. Norovirus and Sapovirus virus-like particles (VLPs)
BRPI0707733B1 (en) 2006-02-13 2019-12-31 Ibio, Inc. isolated antigen, vaccine composition, use of said composition and method for producing an antigen protein
AR060565A1 (en) 2006-04-21 2008-06-25 Dow Agrosciences Llc VACCINE FOR THE AVIARY FLU AND METHODS OF USE
WO2007130327A2 (en) 2006-05-01 2007-11-15 Technovax, Inc. Influenza virus-like particle (vlp) compositions
WO2008054540A2 (en) 2006-05-18 2008-05-08 Pharmexa Inc. Inducing immune responses to influenza virus using polypeptide and nucleic acid compositions
CA2651907C (en) 2006-05-22 2016-12-20 Plant Bioscience Limited Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants
US20070286873A1 (en) 2006-05-23 2007-12-13 Williams John V Recombinant Influenza H5 Hemagluttinin Protein And Nucleic Acid Coding Therefor
EP2120762A1 (en) 2007-01-17 2009-11-25 Lerner Medical Devices, Inc. Fiber optic phototherapy device
US7730950B2 (en) 2007-01-19 2010-06-08 Halliburton Energy Services, Inc. Methods for treating intervals of a subterranean formation having variable permeability
DK2155880T3 (en) 2007-06-15 2016-12-05 Medicago Inc Modification of glycoproteinproduktion i plants
KR100964462B1 (en) 2007-07-10 2010-06-16 성균관대학교산학협력단 Avian Influenza Virus Vaccine Derived from Transgenic Plant and Its Manufacturing Method
WO2009076778A1 (en) 2007-11-27 2009-06-25 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
RU2358981C2 (en) * 2007-08-07 2009-06-20 Центр "Биоинженерия" Российской Академии Наук Universal avian influenza virus vaccine
WO2009026397A2 (en) 2007-08-20 2009-02-26 Fraunhofer Usa, Inc. Prophylactic and therapeutic influenza vaccines, antigens, compositions, and methods
CN101422128B (en) * 2007-10-29 2011-06-08 中国水产科学研究院黄海水产研究所 Separation and regeneration method of gulfweed protoplast
WO2009083420A1 (en) * 2007-12-28 2009-07-09 Unilever Plc Process for recovering aroma from tea
US8799801B2 (en) 2008-01-16 2014-08-05 Qualcomm Incorporated Interactive ticker
SG187500A1 (en) 2008-01-21 2013-02-28 Medicago Inc Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
AU2009267769B2 (en) 2008-07-08 2015-07-16 Medicago Inc. Soluble recombinant influenza antigens
CN101626276A (en) 2008-07-10 2010-01-13 华为技术有限公司 Method, device and system for replacing advertisement
NZ590351A (en) 2008-07-18 2012-11-30 Medicago Inc HA1 domain totally or partially free of N-linked glycosylation operatively linked to a regulatory region
CA2736796A1 (en) 2008-08-27 2010-03-04 Arizona Board Of Regents For And On Behalf Of Arizona State University A dna replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants
WO2010042551A2 (en) * 2008-10-06 2010-04-15 Genvault Corporation Methods for providing cellular lysates from cell wall-containing samples
WO2010077712A1 (en) 2008-12-09 2010-07-08 Novavax, Inc. Bovine respiratory syncytial virus virus-like particle (vlps)
CN102365295A (en) 2009-01-30 2012-02-29 阿德利夫股份有限公司 Conformationally dynamic peptides
CA3018494C (en) 2009-06-10 2021-12-07 Nono Inc. Co-administration of an agent linked to an internalization peptide with an anti-inflammatory
KR101766408B1 (en) 2009-06-10 2017-08-10 알닐람 파마슈티칼스 인코포레이티드 Improved lipid formulation
HUE039100T2 (en) 2009-06-24 2018-12-28 Medicago Inc Chimeric influenza virus-like particles comprising hemagglutinin
KR101773431B1 (en) 2009-09-22 2017-09-12 메디카고 인코포레이티드 Method of preparing plant-derived vlps
WO2012058762A1 (en) 2010-11-04 2012-05-10 Medicago Inc. Plant expression system
KR101847908B1 (en) 2010-11-05 2018-04-11 노바백스, 인코포레이티드 Rabies Glycoprotein Virus-Like Particles(VLPs)
TWI526539B (en) 2010-12-22 2016-03-21 苜蓿股份有限公司 Method for producing viroid-like particles (VLP) in plants and VLP produced by the method
TWI620816B (en) * 2011-03-23 2018-04-11 苜蓿股份有限公司 Plant derived protein recovery method

Also Published As

Publication number Publication date
ES2662778T3 (en) 2018-04-09
WO2011035423A1 (en) 2011-03-31
BR112012006414A2 (en) 2016-11-16
NZ598481A (en) 2014-02-28
US20120178149A1 (en) 2012-07-12
MX2012003372A (en) 2012-05-29
KR20120072371A (en) 2012-07-03
EP2480560A1 (en) 2012-08-01
CN107129973A (en) 2017-09-05
PH12012500565A1 (en) 2012-10-22
CA2772962A1 (en) 2011-03-31
PH12012500566A1 (en) 2012-10-22
RU2567012C2 (en) 2015-10-27
IL218422B (en) 2018-03-29
SG178918A1 (en) 2012-04-27
HUE058165T2 (en) 2022-07-28
CN102549008A (en) 2012-07-04
SI2480560T1 (en) 2018-04-30
JP5554414B2 (en) 2014-07-23
IL218422A0 (en) 2012-04-30
BR112012006414B1 (en) 2020-09-15
KR20180026562A (en) 2018-03-12
PL3354657T3 (en) 2022-05-09
PT3354657T (en) 2022-05-06
AU2010300033B2 (en) 2015-01-22
RU2012115996A (en) 2013-10-27
CA2772962C (en) 2013-04-02
HRP20180509T1 (en) 2018-05-04
DK3354657T3 (en) 2022-04-19
US11833200B2 (en) 2023-12-05
HUE038557T2 (en) 2018-10-29
PH12012500565B1 (en) 2018-01-12
CA2772964A1 (en) 2011-03-31
DK2480658T3 (en) 2017-11-06
ES2911037T3 (en) 2022-05-17
IL218393A0 (en) 2012-04-30
EP3354657B1 (en) 2022-01-26
HRP20220478T1 (en) 2022-05-27
MY164704A (en) 2018-01-30
EP2480658A1 (en) 2012-08-01
AR078433A1 (en) 2011-11-09
KR20190050862A (en) 2019-05-13
KR20120093223A (en) 2012-08-22
IL270661B (en) 2021-12-01
EP3354657A1 (en) 2018-08-01
CN107090021A (en) 2017-08-25
NZ598508A (en) 2014-02-28
MX2012003373A (en) 2012-05-29
MY158475A (en) 2016-10-14
RU2579903C2 (en) 2016-04-10
AU2010300034A1 (en) 2012-03-22
HUE036776T2 (en) 2018-07-30
DK2480560T3 (en) 2018-04-16
KR101773431B1 (en) 2017-09-12
AU2010300033A1 (en) 2012-03-22
ZA201202835B (en) 2015-08-26
IL257261B (en) 2020-03-31
EP2480560B1 (en) 2018-02-21
BR112012006415A2 (en) 2016-11-16
SI3354657T1 (en) 2022-05-31
TR201803560T4 (en) 2019-01-21
HRP20171564T1 (en) 2017-11-17
EP2480658B1 (en) 2017-09-06
RU2012115661A (en) 2013-10-27
NO2480658T3 (en) 2018-02-03
US11826419B2 (en) 2023-11-28
US20130067807A1 (en) 2013-03-21
PT2480560T (en) 2018-04-03
JP2013505272A (en) 2013-02-14
EP2480560A4 (en) 2013-01-23
SG178947A1 (en) 2012-04-27
ZA201202836B (en) 2012-12-27
EP2480658A4 (en) 2013-02-27
PL2480658T3 (en) 2017-12-29
JP2013505025A (en) 2013-02-14
JP5551780B2 (en) 2014-07-16
NO2480560T3 (en) 2018-07-21
SI2480658T1 (en) 2017-11-30
IN2012DN02591A (en) 2015-08-28
BR112012006414A8 (en) 2017-10-10
CN102549148A (en) 2012-07-04
ES2642631T3 (en) 2017-11-17
PL2480560T3 (en) 2018-06-29
AU2010300034B2 (en) 2015-07-30
PT2480658T (en) 2017-11-13
WO2011035422A1 (en) 2011-03-31
CA2772964C (en) 2020-10-27
IN2012DN02637A (en) 2015-09-04
BR112012006415B1 (en) 2020-04-28
AR078432A1 (en) 2011-11-09
KR102115226B1 (en) 2020-05-27

Similar Documents

Publication Publication Date Title
IL257261A (en) Method of preparing plant-derived proteins
JP2013505025A5 (en)
Makhzoum et al. Hairy roots: an ideal platform for transgenic plant production and other promising applications
WO2011062926A3 (en) Methods for enhanced protein production
JP2014510087A5 (en)
CN113754739B (en) A preparation method and application of coronavirus S protein RBD glycoprotein
JP2014505012A5 (en)
Zhang et al. Engineering ‘designer’glycomodules for boosting recombinant protein secretion in tobacco hairy root culture and studying hydroxyproline‐O‐glycosylation process in plants
CN103732758A (en) A low-cost method for expressing and purifying recombinant proteins in plants
CN104010655A (en) Influenza virus-like particles (VLPs) comprising hemagglutinin produced nicotiana tabacum
CN109182232A (en) Recombination bacillus coli zjut-ho1 and its preparing the application in biliverdin
CN104611358A (en) Method for preparing Norovirus virus-like particles in escherichia coli
CN102775466A (en) Preparation method of selenium-containing protein in selenium-enriched yeast
Liang et al. Detection and localization of Rice stripe virus gene products in vivo
CN102925459B (en) PgPDR3 gene and application of encoding protein of PgPDR3 gene in regulating transport and accumulation of ginsenosides
APTROOT Pyrenocarpous lichens and related non-lichenized ascomycetes from Taiwan
CN103834685A (en) Carrier capable of simultaneously improving rosmarinic acid and salvianolic acid B content of red sage root and use thereof
CN109081865A (en) Moso bamboo PeVQ28 albumen and its encoding gene and application
CN108265064B (en) Recombinant artemisia annua 3-class allergen protein and application thereof
CN105802977A (en) Cloning and application of tomato ATG8f gene
CN102250938B (en) A kind of preparation method of sea anemone cardiotonic peptide
US10233264B2 (en) Process for preparing (1→6)-α-D-glucan
CN107227323B (en) Construction and application of high-yield selenomethionine saccharomyces cerevisiae
CN105950644A (en) Chalcone isomerase gene of asparaguses, protein encoded by chalcone isomerase gene and application of chalcone isomerase gene
LU601882B1 (en) A recombinant fowl adenovirus type 11 fiber protein and its preparation method and application

Legal Events

Date Code Title Description
FF Patent granted
KB Patent renewed