IL294765B2 - Deglycosylation methods for electrophoresis of glycosylated proteins - Google Patents

Deglycosylation methods for electrophoresis of glycosylated proteins

Info

Publication number: IL294765B2
Authority: IL; Israel
Prior art keywords: sample; protein; interest; stressed; labeled
Prior art date: 2020-01-21

Application number

IL294765A

Other languages

English (en)

Hebrew (he)

Other versions

IL294765A (en

IL294765B1 (en

Original Assignee

Regeneron Pharma

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2020-01-21

Filing date

2021-01-20

Publication date

2023-12-01

2021-01-20 Application filed by Regeneron Pharma filed Critical Regeneron Pharma

2022-09-01 Publication of IL294765A publication Critical patent/IL294765A/en

2023-08-01 Publication of IL294765B1 publication Critical patent/IL294765B1/en

2023-12-01 Publication of IL294765B2 publication Critical patent/IL294765B2/en

Links

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
- G01N27/041—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a solid body
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
- G01N2440/38—Post-translational modifications [PTMs] in chemical analysis of biological material addition of carbohydrates, e.g. glycosylation, glycation
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Immunology (AREA)
Biomedical Technology (AREA)
Urology & Nephrology (AREA)
Hematology (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Medicinal Chemistry (AREA)
Cell Biology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Food Science & Technology (AREA)
Electrochemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biophysics (AREA)
Bioinformatics & Cheminformatics (AREA)
Bioinformatics & Computational Biology (AREA)
Organic Chemistry (AREA)
Dispersion Chemistry (AREA)
General Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Peptides Or Proteins (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

IL294765A 2020-01-21 2021-01-20 Deglycosylation methods for electrophoresis of glycosylated proteins IL294765B2 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US202062963646P	2020-01-21	2020-01-21
PCT/US2021/014111 WO2021150558A1 (fr)	2020-01-21	2021-01-20	Procédés de déglycosylation pour l'électrophorèse de protéines glycosylées

Publications (3)

Publication Number	Publication Date
IL294765A IL294765A (en)	2022-09-01
IL294765B1 IL294765B1 (en)	2023-08-01
IL294765B2 true IL294765B2 (en)	2023-12-01

Family

ID=74798013

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
IL294765A IL294765B2 (en)	2020-01-21	2021-01-20	Deglycosylation methods for electrophoresis of glycosylated proteins
IL303596A IL303596B2 (en)	2020-01-21	2021-01-20	Deglycosylation methods for electrophoresis of glycosylated proteins

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
IL303596A IL303596B2 (en)	2020-01-21	2021-01-20	Deglycosylation methods for electrophoresis of glycosylated proteins

Country Status (12)

Country	Link
US (2)	US11835527B2 (fr)
EP (2)	EP4085253B1 (fr)
JP (2)	JP7342279B2 (fr)
KR (2)	KR102776242B1 (fr)
CN (2)	CN115398236B (fr)
AU (2)	AU2021209866B2 (fr)
BR (1)	BR112022014212B1 (fr)
CA (1)	CA3165060C (fr)
IL (2)	IL294765B2 (fr)
MX (2)	MX2022008973A (fr)
MY (1)	MY200781A (fr)
WO (1)	WO2021150558A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN115398236B (zh) *	2020-01-21	2024-06-11	瑞泽恩制药公司	用于糖基化的蛋白质的电泳的去糖基化方法
CN114397445A (zh) *	2021-12-23	2022-04-26	上海药明生物医药有限公司	Ce-sds方法优化及其在抗体纯度检测中的应用

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2021
- 2021-01-20 CN CN202180010427.8A patent/CN115398236B/zh active Active
- 2021-01-20 EP EP21708786.5A patent/EP4085253B1/fr active Active
- 2021-01-20 MX MX2022008973A patent/MX2022008973A/es unknown
- 2021-01-20 KR KR1020237019687A patent/KR102776242B1/ko active Active
- 2021-01-20 AU AU2021209866A patent/AU2021209866B2/en active Active
- 2021-01-20 WO PCT/US2021/014111 patent/WO2021150558A1/fr not_active Ceased
- 2021-01-20 JP JP2022544080A patent/JP7342279B2/ja active Active
- 2021-01-20 KR KR1020227027978A patent/KR102544013B1/ko active Active
- 2021-01-20 MY MYPI2022003877A patent/MY200781A/en unknown
- 2021-01-20 IL IL294765A patent/IL294765B2/en unknown
- 2021-01-20 BR BR112022014212-9A patent/BR112022014212B1/pt active IP Right Grant
- 2021-01-20 IL IL303596A patent/IL303596B2/en unknown
- 2021-01-20 EP EP24156921.9A patent/EP4354145B1/fr active Active
- 2021-01-20 US US17/153,150 patent/US11835527B2/en active Active
- 2021-01-20 CN CN202410651573.4A patent/CN118624907A/zh active Pending
- 2021-01-20 CA CA3165060A patent/CA3165060C/fr active Active
2022
- 2022-07-20 MX MX2024008738A patent/MX2024008738A/es unknown
2023
- 2023-08-30 JP JP2023139726A patent/JP7661428B2/ja active Active
- 2023-09-06 AU AU2023226678A patent/AU2023226678B2/en active Active
- 2023-10-26 US US18/495,290 patent/US12480955B2/en active Active

Also Published As

Publication number	Publication date
CA3165060C (fr)	2023-06-20
US11835527B2 (en)	2023-12-05
IL303596B2 (en)	2025-04-01
AU2021209866B2 (en)	2023-07-06
JP2023166478A (ja)	2023-11-21
EP4085253B1 (fr)	2024-03-13
US20210223256A1 (en)	2021-07-22
MX2024008738A (es)	2024-07-23
EP4085253A1 (fr)	2022-11-09
KR102544013B1 (ko)	2023-06-19
WO2021150558A1 (fr)	2021-07-29
BR112022014212B1 (pt)	2024-01-23
CN118624907A (zh)	2024-09-10
CN115398236A (zh)	2022-11-25
IL303596B1 (en)	2024-12-01
AU2021209866A1 (en)	2022-08-18
EP4354145A3 (fr)	2024-07-03
AU2023226678B2 (en)	2025-11-20
US20240053353A1 (en)	2024-02-15
EP4354145A2 (fr)	2024-04-17
US12480955B2 (en)	2025-11-25
IL294765A (en)	2022-09-01
IL303596A (en)	2023-08-01
BR112022014212A2 (pt)	2022-09-20
CN115398236B (zh)	2024-06-11
KR20230088853A (ko)	2023-06-20
KR102776242B1 (ko)	2025-03-07
JP2023506090A (ja)	2023-02-14
MX2022008973A (es)	2022-08-11
EP4354145B1 (fr)	2025-09-17
JP7661428B2 (ja)	2025-04-14
BR122023021668A2 (pt)	2024-01-09
MY200781A (en)	2024-01-15
AU2023226678A1 (en)	2023-09-28
IL294765B1 (en)	2023-08-01
KR20220121892A (ko)	2022-09-01
JP7342279B2 (ja)	2023-09-11
CA3165060A1 (fr)	2021-07-29

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TWI854343B (zh)	2024-09-01	微晶片毛細管電泳分析及試劑
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