IL92079A - Datastoxyclosphorophorin C synthase and its preparation - Google Patents

Datastoxyclosphorophorin C synthase and its preparation

Info

Publication number: IL92079A
Authority: IL; Israel
Prior art keywords: buffer; enzyme; activity; synthase; dna
Prior art date: 1988-10-24

Application number

IL9207989A

Other languages

English (en)

Hebrew (he)

Original Assignee

Lilly Co Eli

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-10-24

Filing date

1989-10-22

Publication date

1994-08-26

1989-10-22 Application filed by Lilly Co Eli filed Critical Lilly Co Eli

1994-08-26 Publication of IL92079A publication Critical patent/IL92079A/en

Links

108030001962 Deacetoxycephalosporin-C synthases Proteins 0.000 title claims description 3
238000002360 preparation method Methods 0.000 title description 5
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108090000790 Enzymes Proteins 0.000 claims description 84
230000000694 effects Effects 0.000 claims description 84
239000000872 buffer Substances 0.000 claims description 79
238000000034 method Methods 0.000 claims description 56
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 44
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 26
239000011780 sodium chloride Substances 0.000 claims description 22
MIFYHUACUWQUKT-UHFFFAOYSA-N Isopenicillin N Natural products OC(=O)C1C(C)(C)SC2C(NC(=O)CCCC(N)C(O)=O)C(=O)N21 MIFYHUACUWQUKT-UHFFFAOYSA-N 0.000 claims description 21
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MIFYHUACUWQUKT-GPUHXXMPSA-N penicillin N Chemical compound OC(=O)[C@H]1C(C)(C)S[C@@H]2[C@H](NC(=O)CCC[C@@H](N)C(O)=O)C(=O)N21 MIFYHUACUWQUKT-GPUHXXMPSA-N 0.000 claims description 20
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

IL9207989A 1988-10-24 1989-10-22 Datastoxyclosphorophorin C synthase and its preparation IL92079A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US07/261,437 US5082772A (en)	1988-10-24	1988-10-24	Process for preparing deacetylcephalosporin c

Publications (1)

Publication Number	Publication Date
IL92079A true IL92079A (en)	1994-08-26

Family

ID=22993294

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL9207989A IL92079A (en)	1988-10-24	1989-10-22	Datastoxyclosphorophorin C synthase and its preparation

Country Status (8)

Country	Link
US (1)	US5082772A (fr)
EP (1)	EP0366354B1 (fr)
JP (1)	JPH02242675A (fr)
DE (1)	DE68925338T2 (fr)
DK (1)	DK521489A (fr)
ES (1)	ES2082787T3 (fr)
GR (1)	GR3018596T3 (fr)
IL (1)	IL92079A (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6709859B1 (en)	1986-01-31	2004-03-23	Beecham Group P.L.C.	Chromosomal DNA Fragments Encoding Enzymes for Encoding B-Lactam Biosynthesis Enzymes, and Vector and Transformants for Their Expression
DE3789880T2 (de) *	1986-01-31	1994-09-08	Beecham Group Plc	Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen.
KR100227711B1 (ko) *	1991-10-15	1999-12-01	한스 발터 라벤	7-아미노세팔로스포란산 및 7-아미노데아세틸세팔로스포란산 제조를 위한 신규한 생물학적 공정
BR9407108A (pt) *	1993-07-30	1996-08-27	Gist Brocades Nv	Processo para a preparação e recuperação de ácido 7-amino desacetoxi cefalosporânico (7-adca) vetor de ADN recombinante e célula hospedeira transformada com um vetor definido
RU2139349C1 (ru) *	1993-07-30	1999-10-10	Гист-Брокейдс Б.В.	Способ эффективного производства 7-адцк через 2-(карбоксиэтилтио) ацетил-7-адцк и 3-(карбоксиметилтио)пропионил-7-адцк
ES2194873T3 (es) *	1993-07-30	2003-12-01	Dsm Ip Assets Bv	Procedimiento para la produccion eficaz de 7-adca a traves de 3-(carboxietil)propionil-7-adca.
US5571910A (en) *	1994-12-09	1996-11-05	Schering Corporation	Process for the preparation of intermediates useful in the synthesis of cephalosporins
US5731165A (en) *	1995-06-02	1998-03-24	Gist-Brocades, B.V.	Process for the production of 7-ADCA via expandase activity on penicillin G
GB2323361A (en) *	1995-11-27	1998-09-23	Gist Brocades Bv	Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin G
AU4114697A (en) *	1996-07-16	1998-02-09	Gist-Brocades B.V.	Improved process for the production of adipoyl cephalosporins
WO1999033994A1 (fr) *	1997-12-24	1999-07-08	Isis Innovation Limited	Desacetoxycephalosporine c synthase (daocs) modifies et structure aux rayons x
US6383773B2 (en) *	1998-04-23	2002-05-07	Massachusetts Institute Of Technology	Penicillin conversion
CN100390285C (zh)	1998-05-19	2008-05-28	Dsm公司	体内生产头孢菌素的改良
DE10051628B4 (de) *	2000-10-18	2007-06-06	Fresenius Hemocare Beteiligungs Gmbh	Einzelsträngiges Oligonukleotid und dessen Verwendung
US6699699B2 (en)	2002-03-26	2004-03-02	Synmax Biochemical Co., Ltd.	Mutated penicillin expandases
CN113234093A (zh) *	2021-04-13	2021-08-10	伊犁川宁生物技术股份有限公司	一种3-去乙酰氧基头孢菌素c钠盐及其制备方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3847742A (en) *	1972-04-26	1974-11-12	C Higgens	Process for deacetoxycephalosporin c
JPS5539735A (en) *	1978-09-11	1980-03-19	Takeda Chem Ind Ltd	Preparation of deacetyl cephalosporin c
US4414328A (en) *	1980-07-21	1983-11-08	Fujisawa Pharmaceutical Co., Ltd.	Process for the preparation of deacetylcephalosporin C
US4510246A (en) *	1982-08-23	1985-04-09	Queen's University At Kingston	Isolation of cyclase, epimerase and a ring expansion enzyme for producing unnatural cephalosporins
US4693977A (en) *	1982-08-23	1987-09-15	Queen's University At Kingston	Enzyme immobilization for producing cephalosporin antibiotics
US4536476A (en) *	1982-08-23	1985-08-20	Queen's University At Kingston	Stable epimerase reagent, cyclase reagent and ring expansion reagent for cell-free production of cephalosporins
DE3789880T2 (de) *	1986-01-31	1994-09-08	Beecham Group Plc	Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen.

1988
- 1988-10-24 US US07/261,437 patent/US5082772A/en not_active Expired - Fee Related
1989
- 1989-10-19 DE DE68925338T patent/DE68925338T2/de not_active Expired - Fee Related
- 1989-10-19 ES ES89310744T patent/ES2082787T3/es not_active Expired - Lifetime
- 1989-10-19 EP EP89310744A patent/EP0366354B1/fr not_active Expired - Lifetime
- 1989-10-20 DK DK521489A patent/DK521489A/da not_active Application Discontinuation
- 1989-10-22 IL IL9207989A patent/IL92079A/en not_active IP Right Cessation
- 1989-10-23 JP JP1275750A patent/JPH02242675A/ja active Pending
1996
- 1996-01-04 GR GR950403475T patent/GR3018596T3/el unknown

Also Published As

Publication number	Publication date
DE68925338T2 (de)	1996-06-05
GR3018596T3 (en)	1996-04-30
JPH02242675A (ja)	1990-09-27
EP0366354B1 (fr)	1996-01-03
DE68925338D1 (de)	1996-02-15
EP0366354A2 (fr)	1990-05-02
DK521489A (da)	1990-04-25
DK521489D0 (da)	1989-10-20
ES2082787T3 (es)	1996-04-01
EP0366354A3 (fr)	1991-03-20
US5082772A (en)	1992-01-21

Legal Events

Date	Code	Title	Description
2000-12-06	RH	Patent void

Publication	Publication Date	Title
US5082772A (en)	1992-01-21	Process for preparing deacetylcephalosporin c
US5070020A (en)	1991-12-03	Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase
Hummel et al.	1985	D-2-Hydroxyisocaproate dehydrogenase from Lactobacillus casei: a new enzyme suitable for stereospecific reduction of 2-ketocarboxylic acids
CA1338642C (fr)	1996-10-15	Methionine-peptidase bacterienne avec n terminal
Poe et al.	1972	Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coli
US5424196A (en)	1995-06-13	Enzymatic process for preparing 7-aminocephalosporanic acid and derivatives
US5013662A (en)	1991-05-07	Bacterial methionine n-terminal peptidase
Riege et al.	1981	Cytochrome P-450 from Lodderomyces elongisporus: its purification and some properties of the highly purified protein
EP0436355B1 (fr)	1996-05-08	Procédé pour produire des composés 7-aminocephem ou leurs sels
US4950603A (en)	1990-08-21	Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from Streptomyces lipmanii
Lucas et al.	1983	Uricase from soybean root nodules: purification, properties, and comparison with the enzyme from cowpea
US4828988A (en)	1989-05-09	Hybrid polypeptides comprising somatocrinine and alpha1 -antitrypsin, method for their production from bacterial clones and use thereof for the production of somatocrinine
US5652132A (en)	1997-07-29	Oxido reductase enzyme system obtainable from P. chrysogenum, the set of genes encoding the same and the use of oxido reductase enzyme systems or genes encoding the same for increasing antibiotic production
US5019509A (en)	1991-05-28	Method and compositions for the production of l-alanine and derivatives thereof
EP0307171A1 (fr)	1989-03-15	Vecteurs d'expression d'ADN recombinant et compositions d'ADN codant pour l'isopénicilline N synthétase d'Aspergillus nidulans
EP0420562B1 (fr)	1995-04-19	Déacétoxycéphalosporine C hydrolase
CA2045838A1 (fr)	1992-01-31	Clonage et surexpression de glucose-6-phosphate dehydrogenase a partir de leuconostoc dextranicus
US4753881A (en)	1988-06-28	Purified enzyme and process therefor
EP0403172A2 (fr)	1990-12-19	Enzyme purifié et procédé à cet effet
Keesey Jr et al.	1981	Subunit structure of anthranilate synthase from Neurospora crassa: preparation and characterization of a protease-free form
US5753435A (en)	1998-05-19	Oxido reductase enzyme system obtainable from P. chrysogenum, the set of genes encoding the same and the use of oxido reductase enzyme systems or genes encoding the same for increasing antibiotic production
JPH07163341A (ja)	1995-06-27	安定化された改変タンパク質