IN2004CH02621A - Low-cost production of peptides - Google Patents

Low-cost production of peptides

Info

Publication number
IN2004CH02621A
IN2004CH02621A IN2621CH2004A IN2004CH02621A IN 2004CH02621 A IN2004CH02621 A IN 2004CH02621A IN 2621CH2004 A IN2621CH2004 A IN 2621CH2004A IN 2004CH02621 A IN2004CH02621 A IN 2004CH02621A
Authority
IN
India
Prior art keywords
peptides
multimers
subject invention
amps
subject
Prior art date
Application number
Inventor
Joseph Krebs
Paul Zorner
Ian Tomlinson
Original Assignee
Dow Global Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Global Technologies Inc filed Critical Dow Global Technologies Inc
Publication of IN2004CH02621A publication Critical patent/IN2004CH02621A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The subject invention relates to a low cost method of producing peptides, including antimicrobial peptides (AMPs), by using microbes. The subject methods enable greatly improved yields of the peptide/AMP as compared to those heretofore known in the art. The subject methods also surprisingly enable the use of Pseudomonas fluorescens to produce AMPs and other peptides. There are several components of the subject invention, which can be used alone or in combination. The subject invention provides for the production of peptides/AMPs in concatemeric precursors. The subject invention also provides novel methods of assembling monomers into multimers, and of cleaving the multimers to yield active monomers. The subject invention also relates to the use of these multimers fused to carrier peptides to produce fusion proteins. Preferably, both the multimers and the fusion proteins (multimers with the carrier polypeptides) lack charge balancing. It has been surprisingly determined that it is not necessary to offset the positive charges of multiple copies of AMPs in multimeric constructs. Thus, the subject invention enables the use of a wider range of multimers and carrier peptides.
IN2621CH2004 2002-04-22 2004-11-22 Low-cost production of peptides IN2004CH02621A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37464402P 2002-04-22 2002-04-22

Publications (1)

Publication Number Publication Date
IN2004CH02621A true IN2004CH02621A (en) 2007-03-02

Family

ID=29251215

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2621CH2004 IN2004CH02621A (en) 2002-04-22 2004-11-22 Low-cost production of peptides

Country Status (11)

Country Link
US (1) US7595173B2 (en)
EP (1) EP1501935B1 (en)
JP (1) JP4472998B2 (en)
KR (1) KR20050016362A (en)
CN (1) CN100489114C (en)
AT (1) ATE443767T1 (en)
AU (1) AU2003228637B2 (en)
CA (1) CA2482995C (en)
DE (1) DE60329382D1 (en)
IN (1) IN2004CH02621A (en)
WO (1) WO2003089455A2 (en)

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JP2019002696A (en) * 2017-06-12 2019-01-10 日本電子株式会社 Mass spectrometry data processing device, mass spectrometry system, and mass spectrometry data processing method
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CN110305890A (en) * 2019-07-02 2019-10-08 河南城建学院 A new method for constructing recombinant engineering bacteria with tandem expression of five antimicrobial peptides and its application
WO2021216621A2 (en) 2020-04-20 2021-10-28 Vestaron Corporation Proteolytically stable u1-agatoxin-ta1b variant polypeptides for pest control
EP4444739A2 (en) 2021-12-08 2024-10-16 Oakgrove Bio LLC Biological production of histidine-rich peptides
CN115083176B (en) * 2022-06-28 2024-05-10 浙江大学 Internet-connected automatic train group serial arrangement realization method based on multitasking parallel control
CN120958034A (en) * 2023-02-23 2025-11-14 微肽技术有限公司 Methods and compositions for producing and purifying peptides
CN116333051A (en) * 2023-03-09 2023-06-27 天津科技大学 Prokaryotic expression preparation method and application of a kind of antimicrobial peptide modified body OM19R
US20260226101A1 (en) * 2023-10-30 2026-08-06 Ramakrishna Reddy ISANAKA Peptide with anti-inflammatory and anti-microbial activity

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Also Published As

Publication number Publication date
AU2003228637A1 (en) 2003-11-03
CA2482995C (en) 2013-01-29
CN1662657A (en) 2005-08-31
JP4472998B2 (en) 2010-06-02
JP2006504400A (en) 2006-02-09
EP1501935B1 (en) 2009-09-23
KR20050016362A (en) 2005-02-21
WO2003089455A3 (en) 2004-05-27
EP1501935A2 (en) 2005-02-02
EP1501935A4 (en) 2007-05-23
DE60329382D1 (en) 2009-11-05
WO2003089455A2 (en) 2003-10-30
CA2482995A1 (en) 2003-10-30
AU2003228637B2 (en) 2010-08-05
US20050227321A1 (en) 2005-10-13
US7595173B2 (en) 2009-09-29
ATE443767T1 (en) 2009-10-15
CN100489114C (en) 2009-05-20

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